期刊
MOLECULAR THERAPY-NUCLEIC ACIDS
卷 12, 期 -, 页码 195-206出版社
CELL PRESS
DOI: 10.1016/j.omtn.2018.05.007
关键词
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资金
- National Scientific Foundation of China [81471508, 81771603]
- Traditional Chinese Medicine Project of Jiangsu Province [ZX2016D2]
- Natural Science Foundation of Jiangsu Province [BK20161061, BK20171502]
- Key Disciplines of 13th Fifth-year Strong and Healthy Engineering in Jiangsu Province
- Research Team of Female Reproductive Health and Fertility Preservation [SZSM201612065]
The long noncoding RNA HOXA11-AS displays abnormal expression in numerous human diseases. However, its function and biological mechanisms remain unclear in preeclampsia (PE). In this study, we report that HOXA11-AS is significantly downregulated in preeclamptic placental tissues and could contribute to the occurrence and development of PE. Silencing of HOXA11-AS expression could significantly suppress trophoblast cell growth and migration, whereas HOXA11-AS overexpression facilitated cell growth in the HTR-8/SVneo, JEG3, and JAR cell lines. RNA-seq analysis also indicated that HOXA11-AS silencing preferentially regulated numerous genes associated with cell proliferation and cell migration. Mechanistic analyses showed that HOXA11-AS could recruit Ezh2 and Lsd1 protein and regulate RND3 mRNA expression in the nucleus. In the cytoplasm, HOXA11-AS modulates HOXA7 expression by sponged miR-15b-5p, affecting trophoblast cell proliferation. Together, these data confirm that aberrant expression of HOXA11-AS is involved in the occurrence and development of PE and may act as a prospective diagnosis and therapeutic target in PE.
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