期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 133, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/57122
关键词
Neuroscience; Issue 133; Microglia; Cell Culture; Defined Medium; Neuroscience; Macrophage; Serum-Free; Ramified; Immunology
资金
- Christopher and Dana Reeve Foundation International Research Consortium on Spinal Cord Injury
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- JPB Foundation
- Novartis Institute of Basic Research
- Damon Runyon Cancer Research Foundation [DRG-2125-12]
Microglia represent 5 - 10% of all central nervous system (CNS) cells and are increasingly drawing attention due to their contributions during development, homeostasis, and disease. Although macrophages have been studied in detail for decades, specialized features of microglia, the tissue-resident macrophages of the CNS, have remained largely mysterious, in part due to limitations in the ability to recapitulate mature microglial properties in culture. Here, we illustrate a straightforward procedure for the rapid isolation of pure microglia from the mature rodent brain. We also describe serum-free culture conditions that support high levels of microglial viability over time. Microglia cultured under these defined-medium conditions exhibit elaborate ramified processes and dynamic surveillance behavior. We illustrate some effects of serum exposure on cultured microglia and discuss how these serum-free cultures compare to both serum-exposed cultures as well as microglia in vivo.
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