Article
Microbiology
Rui Li, Meng Wang, Zhen Ren, Yang Ji, Min Yin, Hao Zhou, Shu-Kun Tang
Summary: A novel actinobacterium, Amycolatopsis aidingensis, was isolated from a saline soil sample in Xinjiang, China. Strain YIM 96748(T) demonstrated high DNA-DNA relatedness and average nucleotide identity, confirming its status as a new species within the Amycolatopsis genus. Further research on this strain is needed to explore its biosynthetic potential for producing secondary metabolites.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hyang-Mi Lee, Jun Ren, Myeong-Sang Yu, Hyunjoo Kim, Woo Young Kim, Junhao Shen, Seung Min Yoo, Seong-il Eyun, Dokyun Na
Summary: By constructing a tunable promoter library, optimization of metabolic engineering in Methylomonas sp. DH-1 was achieved, leading to increased production and a balance between growth and precursor supply.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Ingeborg Bussmann, Fabian Horn, Michael Hoppert, Karl-Walter Klings, Anke Saborowski, Julia Warnstedt, Susanne Liebner
Summary: Three strains of methanotrophic bacteria were isolated from the River Elbe in Germany, including two novel species within the genus Methylomonas and one member of the species Methylovulum psychrotolerans. These bacteria exhibit differences in carbon source utilization and growth conditions, highlighting the importance of identifying and classifying new species in this study.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Environmental Sciences
Mengyuan Qi, Xiaodan Ma, Bin Liang, Liying Zhang, Deyong Kong, Zhiling Li, Aijie Wang
Summary: The study focused on two important degraders of sulfonamide antibiotics, investigating their complete genomes and verifying complete degradation and bacterial growth. Results showed that one of the strains, P27, not only degraded sulfonamide antibiotics but also transformed them into nitrogen-containing heterocyclic products, indicating a broad substrate spectrum and significant bioremediation potential.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Wei Guo, Yang Li, Ronglin He, Wuxi Chen, Feng Gao, Demao Li, Xiaoping Liao
Summary: The study on Methylomonas sp. ZR1 revealed the operation of multiple metabolic pathways including the energy saving ppi-dependent EMP enzyme, complete and efficient central carbon metabolic network, and key formaldehyde metabolism pathway. Additionally, ZR1 showed flexible nitrogen and carotene metabolism patterns when grown on methane, but faced more growth stress when grown on methanol.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Hairong He, Jiarui Huang, Zhenzhu Zhao, Pengqiang Du, Jiansong Li, Jile Xin, Huifang Xu, Weisheng Feng, Xiaoke Zheng
Summary: In this study, a Streptomyces strain RerS4 with strong antifungal activities was isolated from the rhizosphere soil of ring rot-infected Rehmannia glutinosa. The lipopeptide isolated from RerS4 showed restrained proliferation but lacked significant antibacterial and antioxidant activity. Genome analysis revealed the presence of numerous biosynthetic gene clusters in Streptomyces sp. RerS4, suggesting its potential for production of valuable bioactive compounds.
Article
Microbiology
Khorshed Alam, Yiming Zhao, Xuefei Lu, Kai Gong, Lin Zhong, Jinfang Hao, Md. Mahmudul Islam, Saiful Islam, Geng Li, Youming Zhang, Ruijuan Li, Aiying Li
Summary: Burkholderia species have emerged as a new source of natural products, and their genomes contain many biosynthetic gene clusters that can be targeted for drug discovery. The isolation of Burkholderia strain S-53 from soil samples in China revealed its complete genome and the presence of 15 natural product biosynthetic gene clusters, some of which may code for unknown products. This discovery will contribute to the identification of new secondary metabolites and the understanding of BGC regulation.
Article
Virology
Konstantina Katsarou, Christos Andronis, Anthony James, Kostas Euthymiou, Nikoleta Kryovrysanaki, Polykseni G. Pappi, Kriton Kalantidis
Summary: Using high-throughput sequencing, a new carlavirus sequence was identified in a 28-year-old 'Kotsifali' grapevine sample collected in Heraklion, Greece. The complete genome sequence of 8299 nt was confirmed using RT-PCR and 5'/3' RACE with Sanger sequencing, revealing five open reading frames (ORFs) but lacking ORF6 which is present in some Carlavirus members. Based on its similarity to carlaviruses infecting caper, it is proposed to name this new virus as Grapevine Carlavirus 1 according to the ICTV nomenclature.
ARCHIVES OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lan-Fang Li, Qing-Xuan Wu, Hao Wu, Yao Li, Qian Peng, Ren-Hao Han, Da-Hu Zhang, Wei-Dong Yu, Rui Xu, Juan Wang, Zhaobin Fan, Shao-Yang Hou
Summary: Streptomyces sp. HP-A2021, isolated from rhizosphere soil of Ginkgobiloba L., was found to harbor a wealth of cryptic secondary metabolite biosynthetic gene clusters. Genome sequencing and bioinformatics analysis revealed the presence of 33 secondary metabolite biosynthetic gene clusters, including the putative thiotetroamide, alkylresorcinol, coelichelin, and geosmin. Antibacterial activity assays confirmed that HP-A2021 showed potent antimicrobial activity against human pathogenic bacteria.
BIOCHEMICAL GENETICS
(2023)
Article
Agriculture, Dairy & Animal Science
Hae-In Jung, Sungkwon Park, Kai-Min Niu, Sang-Won Lee, Damini Kothari, Kwon Jung Yi, Soo-Ki Kim
Summary: SK3146, a novel strain isolated from pig feed, possesses an immense probiotic potential with a diverse range of hydrolases and bacteriocin encoding gene in its genome sequence.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Beng-Soon Teh, Yiik-Siang Hii, Jamie Hinks, Mohd Firdaus Abdul-Wahab, Sanjay Swarup
Summary: We report the complete genome sequence of Methylomonas sp. UP202 isolated from an urban waterway sediment in Singapore. The genome contains genes involved in methane, methanol, formaldehyde, and formate oxidation, as well as genes utilizing various nitrogen sources.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Chemistry, Multidisciplinary
Chang Keun Kang, Sun-Wook Jeong, Jae-Hwan Jo, Jeong-Ho Park, Min Sik Kim, Jung Eun Yang, Yong Jun Choi
Summary: This study demonstrates the successful bioconversion of methane into squalene using metabolically engineered Methylomonas sp. DH-1 strain, achieving high yield and productivity. The developed strain showed remarkable performance in methane conversion to squalene with significant methane consumption rate, yield, and conversion rate values.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Microbiology
Tao Liu, Zhen Ren, Wei-Xun Chunyu, Gui-Ding Li, Xiu Chen, Zhou-Tian-Le Zhang, Hui-Bing Sun, Mei Wang, Tian-Peng Xie, Meng Wang, Jing-Yuan Chen, Hao Zhou, Zhong-Tao Ding, Min Yin
Summary: The talented endophytic bacteria strain YINM00001 isolated from a Chinese medicinal herb exhibited strong antimicrobial activity and multiple antibiotic resistances. Genomic analysis revealed the potential for producing valuable secondary metabolites, making it an ideal candidate for further biosynthetic studies and production improvement. Further exploration is needed to uncover more novel compounds from this unique Streptomyces strain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Dong Liang, Changyixin Xiao, Fuping Song, Haitao Li, Rongmei Liu, Jiguo Gao
Summary: Pseudomonas sp. DY-1, isolated from contaminated agricultural soil, showed efficient prometryne degradation and potential for plant resistance improvement. Its genome revealed adaptive and beneficial genes related to environmental stress response, metal resistance, and organic compound degradation pathways. The study provides insights into DY-1's application in various fields and contributes to understanding prometryne biodegradation mechanism.
Article
Biotechnology & Applied Microbiology
Wei Guo, Ronglin He, Yujie Zhao, Demao Li
Summary: This study investigates the nitrogen metabolism pattern of the fast-growing bacterium Methylomonas sp. ZR1 and the nitrogen/carbon metabolic balance in single-carbon metabolism. The results show that nitrate salts are the best nitrogen source for ZR1, but the accumulation of the metabolic intermediate ammonium inhibits its growth. The study also reveals that ZR1 employs different strategies to relieve ammonium stress. This research contributes to understanding how nitrogen regulates cellular growth in single-carbon metabolic microorganisms.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Haeyoung Jeong, Siseok Lee, Junsang Ko, Minsu Ko, Hwi Won Seo
Summary: This study presents a bioinformatics approach to extract conserved sequences from SARS-CoV-2 genomes and design diagnostic PCR primers. The results showed that these primers successfully detected different variants of SARS-CoV-2.
Article
Chemistry, Multidisciplinary
Su Yeon Bak, Seung Gi Kang, Kyu Hwan Choi, Ye Rim Park, Eun Yeol Lee, Bum Jun Park
Summary: We describe a phase-transfer biocatalytic process for methane-to-methanol conversion using a spontaneous phase-separable membrane micro-CSTR. The developed system offers several critical advantages for operating the conversion reaction.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Forestry
Yerin Lee, My Ha Tran, Eun Yeol Lee
Summary: In this study, technical lignins were hydroxymethylated to increase their reactivity for synthesis of rigid polyurethane foams. Among the resulting hydroxymethylated lignins, H-A3 lignin showed the highest char yield, thermal stability, and molecular weight. The H-A3 lignin was successfully used to synthesize high-performance polyurethane foams.
EUROPEAN JOURNAL OF WOOD AND WOOD PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Hoa Thi Quynh Le, Dung Hoang Anh Mai, Jeong-Geol Na, Eun Yeol Lee
Summary: In this study, a host strain for violacein production from non-sugar feedstocks, Methylorubrum extorquens AM1, was engineered using metabolic engineering. The results showed that by optimizing expression vectors and inducer concentrations, violacein production using methanol as the sole substrate was achieved. Random mutagenesis and site-directed mutagenesis further improved the production, and co-utilization of methanol and acetate significantly enhanced violacein production. This research provides a platform for economically efficient large-scale fermentation system for violacein production.
METABOLIC ENGINEERING
(2022)
Article
Agricultural Engineering
Hoa Thi Quynh Le, Eun Yeol Lee
Summary: In this study, a unique type I methanotrophic bacterium was used to directly convert propane to pure 2-propanol without the need of chemical inhibitors or additional reducing-power sources. By manipulating the genetic background and optimizing cultivation conditions, the researchers achieved a significant increase in the production of 2-propanol.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Tin Hoang Trung Chau, Anh Duc Nguyen, Eun Yeol Lee
Summary: This study demonstrated that M. alcaliphilum 20Z can co-utilize methane and xylose from anthropogenic activities and lignocellulose biomass to produce (R)-3-hydroxybutyrate and poly (3-hydroxybutyrate). The production of (R)-3-hydroxybutyrate was optimized by introducing additional thioesterase and a tunable genetic module. The study also highlighted the importance of pathway reversibility as a design strategy for balancing driving force and intermediate accumulation.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
My Ha Tran, Boreum Lee, Hyunjun Lee, Boris Brigljevic, Eun Yeol Lee, Hankwon Lim
Summary: This study conducted an integrated techno-economic and life cycle assessment for two-step solvothermal liquefaction and one-step solvothermal liquefaction based on experiment results. The results showed that the one-step synthesis had lower unit production costs and slightly lower greenhouse gas emissions compared to the two-step synthesis. The analytic hierarchy process was used to determine the best process for biopolyol production based on different weight points in the economic and environmental aspects.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Agricultural Engineering
Hoa Thi Quynh Le, Eun Yeol Lee
Summary: The study explores the metabolic pathways of methane and methanol to produce 1,2-propanediol (1,2-PDO) using the type I methanotroph Methylotuvimicrobium alcaliphilum 20Z. It finds that the carbon flux from methane to the EMP pathway restricts the production of 1,2-PDO. Co-utilization of C1 substrates and glycerol enhances the production of 1,2-PDO, although lactate is co-produced in higher amounts. The study provides insights into metabolic routes for 1,2-PDO and lactate co-production from C1 and C3 compounds in methanotrophs.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Jiyeong Jeong, Tae Hyun Kim, Nulee Jang, Minji Ko, Seong Keun Kim, Georgii Emelianov, Eugene Rha, Kil Koang Kwon, Haseong Kim, Eun Yeol Lee, Dae-Hee Lee, Hyewon Lee, Seung-Goo Lee
Summary: Methanotrophs have been successfully engineered to produce valuable bioproducts from methane by utilizing a phenol-inducible gene expression system and a CRISPR-based genome editing tool. The engineered strain achieved the highest concentration of synthetic biochemicals produced from methane in methanotrophs.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Engineering, Environmental
Diep Ngoc Pham, Anh Duc Nguyen, Eun Yeol Lee
Summary: Methane and carbon dioxide are the most abundant greenhouse gases, and reducing their emissions is crucial for mitigating climate change. Utilizing methylotrophic bacteria-based technologies to capture and utilize these gases shows promise in lowering their concentrations. This article highlights the advantages of methylotrophs in bioconversion of methane, CO2, methanol, and formate, and discusses innovative strategies to improve conversion efficiency.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Ushasree Mrudulakumari Vasudevan, Dung Hoang Anh Mai, Shyam Krishna, Eun Yeol Lee
Summary: Methanotrophs have the potential to produce various bioactive secondary metabolites including iso-prenoids, polymers, peptides, and vitamins. Genome mining of these microbes revealed the hidden biosynthetic potential of these microbes. Through advanced synthetic biology tools, the central carbon metabolism in methanotrophs can be modified to produce valuable secondary metabolites. This review summarizes the possibilities of developing methanotrophs as microbial chassis for high-value secondary metabolites.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Chemistry, Multidisciplinary
My Ha Tran, Eun Yeol Lee
Summary: This article reviews the methods for producing polyols from vegetable oil, lignin, and carbohydrates, as well as the production of polyurethane from biomass polyols. Conversion of vegetable oil involves epoxidation, hydroformylation, and transesterification, while conversion of lignin involves solvothermal liquefaction, hydroxymethylation, oxypropylation, and phenolation.
ENVIRONMENTAL CHEMISTRY LETTERS
(2023)
Article
Multidisciplinary Sciences
Sanzhar Naizabekov, Seung Woon Hyun, Jeong-Geol Na, Sukhwan Yoon, Ok Kyung Lee, Eun Yeol Lee
Summary: Comparative genomic analysis revealed the potential of newly isolated methanotroph Methylocystis sp. MJC1 as a biodegradable polyhydroxybutyrate (PHB) producer. Under nitrogen-limiting conditions, Methylocystis sp. MJC1 can accumulate up to 44.5% PHB based on dry cell weight. Functional annotation and comparison with other Methylocystis species showed that Methylocystis sp. MJC1 contains all major biochemical pathways of type II methanotrophs and possesses both particulate and soluble methane monooxygenases, which are not commonly found in other Methylocystis species. These findings open up possibilities for future practical applications such as organism-specific genome-scale models and metabolic engineering strategies.
Article
Chemistry, Multidisciplinary
Dieu-Phuong Phan, My Ha Tran, Eun Yeol Lee
Summary: Polymeric materials play a crucial role in modern society, and biomass can be utilized as a renewable carbon source for the production of bio-based plastics. This review summarizes the applications of Metal-Organic Framework (MOF)-based catalysts in the production of renewable bio-plastic-derived materials and highlights the effects of reaction conditions.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Arslan Sarwar, Eun Yeol Lee
Summary: Methanol is gaining attention as a potential carbon substrate for the production of fuels and chemicals due to its high reduction degree, abundance, and low price. Both native and synthetic methylotrophic microorganisms have been investigated for the production of biofuels and chemicals. However, the complex metabolic pathways, limited availability of genetic tools, and toxicity issues still pose challenges for achieving high-level production for commercial purposes. This article reviews the production of biofuels and chemicals from methanol by native and synthetic methylotrophs and discusses ways to improve their efficiency.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
