期刊
GENETICS
卷 209, 期 4, 页码 1055-1071出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.118.301116
关键词
transcription; enhancer; sequence-level instructions; immune response
资金
- Centre National de la Recherche Scientifique (CNRS), Plan d'Investissement d'Avenir Institut de Biologie Computationnelle [ANR-11-BINF-0002]
- Genome Canada/Genome BC Large Scale Applied Research Grant [174CDE]
- Child and Family Research Institute
- British Columbia Children's Hospital Foundation, Vancouver
- Norwegian Research Council [187615]
- Helse Sor-Ost
- University of Oslo through the Centre for Molecular Medicine Norway (NCMM)
- Oslo University Hospital Radiumhospitalet
The FANTOM5 consortium recently characterized 65,423 human enhancers from 1829 cell and tissue samples using the Cap Analysis of Gene Expression technology. We showed that the guanine and cytosine content at enhancer regions distinguishes two classes of enhancers harboring distinct DNA structural properties at flanking regions. A functional analysis of their predicted gene targets highlighted one class of enhancers as significantly enriched for associations with immune response genes. Moreover, these enhancers were specifically enriched for regulatory motifs recognized by transcription factors involved in immune response. We observed that enhancers enriched for links to immune response genes were more cell-type specific, preferentially activated upon bacterial infection, and with specific response activity. Looking at chromatin capture data, we found that the two classes of enhancers were lying in distinct topologically associating domains and chromatin loops. Our results suggest that specific nucleotide compositions encode for classes of enhancers that are functionally distinct and specifically organized in the human genome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据