Article
Environmental Sciences
Gangqiang An, Minfeng Xing, Binbin He, Haiqi Kang, Jiali Shang, Chunhua Liao, Xiaodong Huang, Hongguo Zhang
Summary: This study utilized multitemporal hyperspectral UAV data to identify sensitive wavebands for rice false smut (RFS) and extract RFS-infected areas using two methods. The results showed that the second method had better prediction accuracy and that the infected areas exhibited an expanding trend over time, consistent with the natural development law of RFS.
Article
Environmental Sciences
Qian Sun, Hao Liu, Yongkang Zhang, Xun 'e Yi, Ren Kong, Shiyang Cheng, Jianguo Man, Lu Zheng, Junbin Huang, Guanyong J. Su, Robert P. Letcher, John P. Gisy, Chunsheng Liu
Summary: This study for the first time reports the widespread geographical distribution of ustiloxins in rice and emphasizes the occurrence of internal exposure and potential health risks in humans.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Environmental Sciences
Qian Sun, Zhisong Qian, Hao Liu, Yongkang Zhang, Xun'e Yi, Ren Kong, Shiyang Cheng, Jianguo Man, Lu Zheng, Junbin Huang, Guanyong Su, Robert J. Letcher, John P. Giesy, Chunsheng Liu
Summary: Ustiloxin A and ustiloxin B, two major mycotoxins produced by the pathogen of rice false smut, were found to be ubiquitous in paddy water and brown rice, with a significant positive correlation between their levels. These results indicate that the contamination of ustiloxins in rice might occur widely.
ENVIRONMENTAL POLLUTION
(2022)
Article
Agronomy
Enlong Shen, Xingchao Wang, Zhaoxi Lu, Fei Zhou, Weihua Ma, Zhongli Cui, Zhoukun Li, Changyan Li, Yongjun Lin
Summary: Researchers transferred a codon-optimized beta-1,6-glucanase gene (GluM) from myxobacteria into the japonica rice variety Zhonghua11 (ZH11), resulting in transgenic rice plants with GluM overexpression. These transgenic plants exhibited broad-spectrum disease resistance, with significant reductions in the areas of rice blast and sheath blight lesions, enhanced sheath blight resistance in the field, and decreased incidence of diseased panicles due to rice false smut. Furthermore, there was no observable loss of yield, and plants overexpressing GluM showed higher disease resistance compared to plants overexpressing a beta-1,3-glucanase gene derived from tobacco.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yu Zhang, Xinyue Li, Shuya Zhang, Tianling Ma, Chengxin Mao, Chuanqing Zhang
Summary: A quantitative loop-mediated isothermal amplification (q-LAMP) method was developed for Ustilaginoidea virens detection and quantification, which is more sensitive and efficient than quantitative real-time PCR (q-PCR) method. The q-LAMP assay could detect a concentration of 6.4 spores/mL at an optimal reaction temperature of 63.4℃ within 60 minutes. It is a powerful and simple monitoring tool for U. virens, providing valuable technical support for the forecast and management of rice false smut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Niqing He, Fenghuang Huang, Libin Lu, Xun Wang, Qingshun Q. Q. Li, Dewei Yang
Summary: The study found that the variation in panicle shape in rice has a significant impact on resistance to false smut. Through cloning and functional characterization, it was discovered that the SPR9 gene, which encodes a 60 S ribosomal protein, regulates panicle shape and affects resistance to false smut in rice.
Article
Food Science & Technology
Manas Kumar Bag, Nabaneeta Basak, Torit Bagchi, Prahlad Masurkar, Anuprita Ray, Totan Adak, Mayabini Jena, Prakash Chandra Rath
Summary: The false smut disease caused by Ustilaginoidea virens in rice has become a global concern, with significant impact on yield, cooking quality, and nutritional properties of various Indian rice varieties. The disease results in reduced protein content, increased amylose content, and decreased antioxidant activity in affected grains, highlighting the importance of maintaining rice grain quality in combating the disease.
JOURNAL OF CEREAL SCIENCE
(2021)
Article
Entomology
Mathew S. Baite, S. R. Prabhukarthikeyan, S. Raghu
Summary: This study evaluated the effectiveness of various biocontrol agents in controlling false smut disease in rice. It was found that Trichoderma harzianum and Bacillus subtilis showed the highest potential in controlling the disease. In addition to disease suppression, these biocontrol agents also improved rice yield.
Article
Food Science & Technology
Yi Huang, Xiaoqian Tang, Lu Zheng, Junbin Huang, Qi Zhang, Hao Liu
Summary: Ustiloxins are a group of mycotoxins produced by rice false smut pathogen, and there is an urgent need for immunoassay methods for their detection. This study developed a generic antigen and antibody based on ustiloxin G to improve the efficiency of ustiloxin detection, showing promising results for on-site monitoring.
Article
Plant Sciences
Xiaoxiang Fu, Yu Jin, Matthew J. Paul, Minxuan Yuan, Xingwei Liang, Ruqiang Cui, Yingjin Huang, Wenwen Peng, Xiaogui Liang
Summary: Ustiloxins are the main mycotoxin in rice false smut, causing inhibition of seed germination. In this study, the authors found that ustiloxin A (UA) inhibits rice germination in a dose-dependent manner. UA treatment resulted in decreased sugar availability and increased starch residue in the embryo. The expression of sugar transport genes was down-regulated, and carbon metabolism and amino acid utilization were altered in rice plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Correction
Biochemical Research Methods
Ning Yang, Yuanyuan Ji, Aiying Wang, Jian Tang, Shuhua Liu, Xiaodong Zhang, Lijia Xu, Yong He
Summary: Correction is provided for the integrated nucleic acid detection method based on a microfluidic chip for collection and culture of rice false smut spores. The corrected method allows efficient collection and culture of rice false smut spores, enabling rapid and sensitive nucleic acid detection.
Article
Multidisciplinary Sciences
Matthias Kretschmer, Djihane Damoo, Sherry Sun, Christopher W. J. Lee, Daniel Croll, Harry Brumer, James Kronstad
Summary: This study reveals that combinations of organic acids and glucose can trigger late-stage phenotypes associated with the biotrophic lifestyle in the maize pathogen Ustilago maydis, including the expression of effectors and melanin formation. This response to combinations of nutrients may be a conserved feature of fungal biotrophy.
Article
Plant Sciences
Jiandong Bao, Rong Wang, Shilei Gao, Zhe Wang, Yu Fang, Lv Wu, Mo Wang
Summary: In this study, a near-complete genome assembly of U. virens isolate UV-FJ-1 was generated using PacBio Sequel II HIFI-read sequencing technology, revealing a large number of protein-coding genes and secondary metabolite biosynthesis gene clusters, which will be valuable resources for further understanding the biological and pathogenic mechanisms of this fungus.
Review
Plant Sciences
Dewei Yang, Niqing He, Fenghuang Huang, Yidan Jin, Shengping Li
Summary: Rice false smut (RFS), caused by Ustilaginoidea virens, is a devastating disease worldwide, resulting in significant yield loss and health threats due to mycotoxin production. This review updates the understanding of RFS symptoms, resistance genes, genomics, effectors, and immune response mechanisms. Additionally, it explores the identification methods, breeding for resistance, and interactions between effector proteins and resistance genes, which can contribute to the development of disease-resistant materials in rice breeding programs.
Article
Plant Sciences
Tao Yin, Qiang Zhang, Jianhua Wang, Huiquan Liu, Chenfang Wang, Jin-Rong Xu, Cong Jiang
MOLECULAR PLANT PATHOLOGY
(2018)
Article
Multidisciplinary Sciences
Huiquan Liu, Yang Li, Daipeng Chen, Zhaomei Qi, Qinhu Wang, Jianhua Wang, Cong Jiang, Jin-Rong Xu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Plant Sciences
Qinhu Wang, Cong Jiang, Chenfang Wang, Changjun Chen, Jin-Rong Xu, Huiquan Liu
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Manli Sun, Yimei Zhang, Qinhu Wang, Chunlan Wu, Cong Jiang, Jin-Rong Xu
MOLECULAR MICROBIOLOGY
(2018)
Article
Microbiology
Cong Jiang, Xue Zhang, Huiquan Liu, Jin-Rong Xu
Article
Plant Sciences
Qinhu Wang, Daipeng Chen, Mengchun Wu, Jindong Zhu, Cong Jiang, Jin-Rong Xu, Huiquan Liu
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Microbiology
Qinhu Wang, Huiquan Liu, Huaijian Xu, Ruonan Hei, Shijie Zhang, Cong Jiang, Jin-Rong Xu
ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Microbiology
Jingyi Ren, Chengliang Li, Chengyu Gao, Jin-Rong Xu, Cong Jiang, Guanghui Wang
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Cong Jiang, Shulin Cao, Zeyi Wang, Huaijian Xu, Jie Liang, Huiquan Liu, Guanghui Wang, Mingyu Ding, Qinhu Wang, Chen Gong, Chanjing Feng, Chaofeng Hao, Jin-Rong Xu
NATURE MICROBIOLOGY
(2019)
Review
Genetics & Heredity
Meng Ye, Hang Jiang, Xianhui Fu, Jin-Rong Xu, Cong Jiang
Summary: Histone modifications form networks regulating DNA accessibility and transcription, with ING proteins linked to this process; Fng1 in fungi is involved in crosstalk among histone acetylation, deacetylation, and methylation.
Article
Plant Sciences
Ping Lu, Daipeng Chen, Zhaomei Qi, Haoming Wang, Yitong Chen, Qinhu Wang, Cong Jiang, Jin-Rong Xu, Huiquan Liu
Summary: This study investigates the landscape and regulation of alternative splicing (AS) and alternative polyadenylation (APA) in Fusarium graminearum, a filamentous fungus. The results reveal that AS and APA play important roles in gene expression regulation, with the majority of AS events affecting coding sequences. Unexpectedly, AS transcripts with premature-termination codons are not degraded. Distal APA sites have strong signals, while proximal APA isoforms are highly expressed in F. graminearum. The study also uncovers the roles of 3'-end processing factors in promoting proximal APA site usage and intron splicing. Furthermore, the study suggests intron inclusion and 3'-untranslated region lengthening as novel mechanisms in regulating aging and dormancy in fungi.
Article
Plant Sciences
Huaijian Xu, Meng Ye, Aliang Xia, Hang Jiang, Panpan Huang, Huiquan Liu, Rui Hou, Qinhu Wang, Dongao Li, Jin-Rong Xu, Cong Jiang
Summary: Fng3 is a protein that interacts with histone modification complexes, involved in the regulation of histone acetylation, and crucial for fungal development and pathogenicity.
Review
Plant Sciences
Ming Xu, Qinhu Wang, Guanghui Wang, Xue Zhang, Huiquan Liu, Cong Jiang
Summary: This review discusses the devastating impact of Fusarium head blight (FHB) caused by Fusarium graminearum on wheat and barley, as well as recent advances in understanding the pathogenic mechanisms, resistance genes, and breeding of resistant varieties. A comprehensive understanding of the molecular interactions between F. graminearum and wheat will contribute to the development of novel and efficient strategies for controlling FHB.
PHYTOPATHOLOGY RESEARCH
(2022)
Article
Genetics & Heredity
Hang Jiang, Aliang Xia, Meng Ye, Jingyi Ren, Dongao Li, Huiquan Liu, Qinhu Wang, Ping Lu, Chunlan Wu, Jin-Rong Xu, Cong Jiang