Article
Biochemistry & Molecular Biology
Congcong Jiang, Miaomiao Lei, Yu Guo, Guangqi Gao, Lijie Shi, Yanlong Jin, Yu Cai, Axel Himmelbach, Shenghui Zhou, Qiang He, Xuefeng Yao, Jinhong Kan, Georg Haberer, Fengying Duan, Lihui Li, Jun Liu, Jing Zhang, Manuel Spannagl, Chunming Liu, Nils Stein, Zongyun Feng, Martin Mascher, Ping Yang
Summary: In this study, we created an EMS-mutagenized population of barley and established a highly efficient and cost-effective TILLING platform using a three-dimensional DNA pooling strategy and multiplexed amplicon sequencing. We successfully identified abundant allelic mutants for various genes, including the BRI1 gene. Additionally, we demonstrated the value of this resource by rapidly determining the causal gene responsible for a chlorotic mutant.
PLANT COMMUNICATIONS
(2022)
Review
Plant Sciences
Miriam Szurman-Zubrzycka, Marzena Kurowska, Bradley J. Till, Iwona Szarejko
Summary: Since its introduction in 2000, the TILLING strategy has been widely used in plant research to create novel genetic diversity. TILLING mutants may be used for functional analysis of genes and being nontransgenic, they may be directly used in pre-breeding programs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yanli Wang, David E. Salt, Maarten Koornneef, Mark G. M. Aarts
Summary: A mutant library for Noccaea caerulescens was successfully established using a non-transgenic approach, providing a useful resource for forward mutant screens and TILLING. Mutants with altered mineral concentrations were identified through ionomics screening.
Review
Biotechnology & Applied Microbiology
Dharmendra Singh, Priya Chaudhary, Jyoti Taunk, Chandan Kumar Singh, Viswanathan Chinnusamy, Amitha Mithra Sevanthi, Vikram Jeet Singh, Madan Pal
Summary: Conventional crop breeding alone cannot solve global hunger, but the development of high throughput reverse genetics approaches, such as TILLING, has greatly benefited plant breeding programs. TILLING employs mutagenesis to create new genetic sources and identify superior alleles. Modifications to the original TILLING protocol, such as EcoTILLING and De-TILLING, have widened its applicability to different crops. Combining next generation sequencing with TILLING allows for efficient screening of germplasm collections and mutant populations.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Plant Sciences
Mohsen Niazian, Francois Belzile, Davoud Torkamaneh
Summary: Sequence and expression data obtained by next-generation sequencing (NGS)-based forward genetics methods allow the identification of candidate causal genes. Reverse genetics techniques, such as CRISPR/Cas9, are valuable for providing experimental evidence of a gene's function.
Article
Virology
Yingying Lei, Yu Xiong, Dagang Tao, Tao Wang, Tianlun Chen, Xufei Du, Gang Cao, Jiagang Tu, Jinxia Dai
Summary: In this study, a reverse genetic system for the culture of recombinant red-spotted grouper nervous necrosis virus (RGNNV) is developed for the first time. Attenuated RGNNV strains with the loss of B2 protein expression were constructed and compared in terms of growth rate, Mx1 expression, and virulence. This study provides a potential tool for further research on viral protein function, virulence pathogenesis, and vaccine development of RGNNV.
Article
Plant Sciences
Mingjiu Li, Goetz Hensel, Michael Melzer, Astrid Junker, Henning Tschiersch, Hannes Ruwe, Daniel Arend, Jochen Kumlehn, Thomas Boerner, Nils Stein
Summary: Research on barley ohnologous genes ALBOSTRIANS and ALBOSTRIANS-LIKE revealed their involvement in CCT domain proteins, with HvCMF7 related to chloroplast ribosome biogenesis and HvCMF3 mutations leading to delayed seedling development with distinctive leaf color changes.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Environmental Sciences
Lisa T. van der Heijden, Karen E. van den Hondel, Erik J. H. Olyslager, Lutea A. A. de Jong, Udo J. L. Reijnders, Eric J. F. Franssen
Summary: There has been a significant increase in sodium azide intoxications since the 1980s, particularly in the Netherlands where it is promoted for self-euthanasia. The presented case describes a suicide using sodium azide by a young woman with a history of depression. Within the community promoting sodium azide, it is recommended to combine it with medications targeting its negative effects.
Article
Multidisciplinary Sciences
Olga Wachelko, Marcin Zawadzki, Pawel Szpot
Summary: This study investigated the epidemiology of sodium azide exposures and developed a simple, accurate, and selective detection method. Additionally, a technique for stabilizing sodium azide was presented, which has great potential for detecting the substance in clinical and toxicology laboratories even long after exposure.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
D. Pellerin, M. C. Danzi, C. Wilke, M. Renaud, S. Fazal, M. -J Dicaire, C. K. Scriba, C. Ashton, C. Yanick, D. Beijer, A. Rebelo, C. Rocca, Z. Jaunmuktane, J. A. Sonnen, R. Lariviere, D. Genis, L. Molina Porcel, K. Choquet, R. Sakalla, S. Provost, R. Robertson, X. Allard-Chamard, M. Tetreault, S. J. Reiling, S. Nagy, V Nishadham, M. Purushottam, S. Vengalil, M. Bardhan, A. Nalini, Z. Chen, J. Mathieu, R. Massie, C. H. Chalk, A. -L Lafontaine, F. Evoy, M. -F Rioux, J. Ragoussis, K. M. Boycott, M. -P Dube, A. Duquette, H. Houlden, G. Ravenscroft, N. G. Laing, P. J. Lamont, M. A. Saporta, R. Schuele, L. Schoels, R. La Piana, M. Synofzik, S. Zuchner, B. Brais
Summary: A repeat expansion in the FGF14 gene was found to be associated with late-onset cerebellar ataxias (LOCAs) in French Canadian and German populations as well as in Australian and Indian patients. This discovery provides important insights for further research and diagnosis of LOCAs.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Chemistry, Applied
Kursat Karaman, Sibel Kizil, Merve Basak, Bulent Uzun, Engin Yol
Summary: Reducing allergenicity and increasing oleic content are important goals in groundnut breeding studies. A mutant population induced by EMS was generated to alter functional properties of major allergen gene Ara h 1 and oleic acid converting gene FAD2 in tetraploid groundnut through TILLING approach.
JOURNAL OF OLEO SCIENCE
(2021)
Article
Immunology
Fuxiao Liu, Jiahui Lin, Qianqian Wang, Youming Zhang, Hu Shan
Summary: Canine distemper and canine parvoviral enteritis are two common infectious diseases in dogs, caused by canine distemper virus and canine parvovirus type 2, respectively. Researchers successfully generated a recombinant CDV expressing VP2 protein, but a nonsense mutation occurred in VP2 ORF during passage and became predominant. Next-generation sequencing identified multiple single-nucleotide variations, including nonsense and missense mutations.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Katarina Valentovicova, Loriana Demecsova, Lubica Liptakova, Veronika Zelinova, Ladislav Tamas
Summary: The use of NBT and HE is efficient for detecting superoxide in intact barley root tips, while DCDHF-DA and DHR123 are suitable for the detection of peroxynitrite, but only if PRXs and oxidoreductases are inhibited to prevent false-positive reactions.
Article
Agronomy
Anusheela Varadaraju, Bharathi Raja Ramadoss, Selvakumar Gurunathan, Ramya Balaram, Kulandaivelu Ganesamurthy, Sundaram Ganesh Ram
Summary: Mutation is essential for creating new variations in crop plants, as seen in a study involving mutated mungbean plants. Specific gene mutations were found to alter plant architecture and potentially increase yield. Selected mutants with desirable traits are being further studied for potential contribution in developing high-yielding mungbean genotypes.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Plant Sciences
Xiao-Dong Fang, Ji-Hui Qiao, Ying Zang, Qiang Gao, Wen-Ya Xu, Dong-Min Gao, Yi-Zhou Yang, Liang Xie, Ying Wang, Xian-Bing Wang
Summary: In this study, a minireplicon system of Northern cereal mosaic virus (NCMV) was developed and a recombinant virus was successfully recovered in small brown planthoppers and barley plants. Cellular superinfection exclusion was observed in barley leaves between NCMV and barley yellow striate mosaic virus (BYSMV), indicating that the two insect-transmitted cytorhabdoviruses are enemies at the cellular level during coinfections in plants.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Ecology
Anete Borodus, Juris Nikolajeva, Davids Fridmanis, Dita Gudra, Maija Ustinova, Maris Senkovs, Vizma Nikolajeva
Summary: The study demonstrates that PNA clamping can reduce host-plant co-amplification in fungal endophyte community sequencing, but it does not improve sequencing metrics when specific primers are already excluding host DNA amplification.
Review
Plant Sciences
Heribert Hirt, Salim Al-Babili, Marilia Almeida-Trapp, Antoine Martin, Manuel Aranda, Dorothea Bartels, Malcolm Bennett, Ikram Blilou, Damian Boer, Alix Boulouis, Chris Bowler, Sophie Brunel-Muguet, Fabien Chardon, Jean Colcombet, Vincent Colot, Agata Daszkowska-Golec, Jose R. Dinneny, Ben Field, Katja Froehlich, Catherine H. Gardener, Alain Gojon, Eric Gomes, Eva Maria Gomez-Alvarez, Crisanto Gutierrez, Michel Havaux, Scott Hayes, Edith Heard, Michael Hodges, Amal Khalaf Alghamdi, Laurent Laplaze, Kyle J. Lauersen, Nathalie Leonhardt, Xenie Johnson, Jonathan Jones, Hannes Kollist, Stanislav Kopriva, Anne Krapp, Mauricio Lopez-Portillo Masson, Matthew F. McCabe, Livia Merendino, Antonio Molina, Jose L. Moreno Ramirez, Bernd Mueller-Roeber, Michael Nicolas, Ido Nir, Izamar Olivas Orduna, Jose M. Pardo, Jean-Philippe Reichheld, Pedro L. Rodriguez, Hatem Rouached, Maged M. Saad, Peter Schloegelhofer, Kirti A. Singh, Ive De Smet, Clara Stanschewski, Alice Stra, Mark Tester, Catherine Walsh, Andreas P. M. Weber, Detlef Weigel, Philip Wigge, Michael Wrzaczek, Brande B. H. Wulff, Iain M. Young
Summary: Greenhouse gas emissions are causing a global climate crisis, and immediate interventions are needed to mitigate their negative effects. Plant scientists are crucial in finding solutions for sustainable agriculture and land use, as it contributes significantly to total GHG emissions. The PlantACT! (Plants for climate ACTion!) initiative lays out a road map for plant scientists to contribute to finding immediate, mid-term, and long-term solutions, and highlights necessary changes at personal, institutional, and funding levels to implement these solutions.
TRENDS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Agata Daszkowska-Golec, Martin Mascher, Runxuan Zhang
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Jevgenija Necajeva, Anete Boroduske, Vizma Nikolajeva, Maris Senkovs, Ineta Kalnina, Ance Roga, Edmunds Skinderskis, Davids Fridmanis
Summary: This study isolated fungi from the surface and endosphere of two widespread Poaceae weed species, Avena fatua and Echinochloa crus-galli, to compare their taxonomic composition. The seed-degrading potential of Fusarium isolated from the seeds of E. crus-galli was tested. Molecular identification of epiphytic and endophytic fungal genera was performed. Endophytes had significantly lower fungal richness compared to epiphytes. There was a significant taxonomic overlap between the endosphere and seed surface, with Alternaria, Fusarium, Cladosporium, and Sarocladium being the most abundant genera. Microbial communities showed greater dissimilarity between the two plant species than between the years. Fusarium isolates F. sporotrichioides and F. culmorum demonstrated high potential to infect and degrade E. crus-galli seeds under laboratory conditions.
Editorial Material
Plant Sciences
Ewa Sybilska, Agata Daszkowska-Golec
Summary: Mei et al. recently discovered the molecular mechanism behind the synergistic action of auxins and jasmonates in enhancing the role of abscisic acid (ABA) in seed germination. They found that JASMONATE-ZIM DOMAIN (JAZ) proteins interact with AUXIN RESPONSE FACTOR (ARF)-16 to mediate auxin-jasmonic acid (JA) crosstalk. Furthermore, they revealed that ARF16 interacts with ABSCISIC ACID INSENSITIVE (ABI)-5 and positively modulates ABA responses at seed germination.
TRENDS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Damian Gruszka, Andrzej Bajguz, Qian-Feng Li, Shamsul Hayat, Mats Hansson
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Yanka Mihailova, Krasimir Rusanov, Mila Rusanova, Pavlina Vassileva, Ivan Atanassov, Vasil Nikolov, Elena G. G. Todorovska
Summary: This study used microsatellite (SSR) markers to investigate the genetic status of 12 Bulgarian autochthonous sheep breeds. A total of 600 individuals from 50 flocks were analyzed, and 228 alleles were found. The results showed low differentiation between the breeds, with two breeds being the most distinct and the remaining breeds showing poor breed differentiation. Proper management strategies and breeding policies are needed to avoid intermixing of breeds and preserve breed purity.
Article
Plant Sciences
Agata Daszkowska-Golec, Devang Mehta, R. Glen Uhrig, Agnieszka Braszewska, Ondrej Novak, Irene M. Fontana, Michael Melzer, Tomasz Plociniczak, Marek Marzec
Summary: We used a combination of transcriptomics, proteomics, and phytohormonomics analyses to investigate the SL-dependent mechanisms associated with water deficit response in barley. Our research revealed the role of SLs in alleviating the effects of water limitation on plants and identified potential strategies for developing drought-resistant barley.
Article
Biochemistry & Molecular Biology
Sunny Ahmar, Karolina Zolkiewicz, Damian Gruszka
Summary: Plant Glycogen Synthase Kinases (GSKs) play a crucial role in regulating various physiological processes by enabling crosstalk among signaling pathways. However, the mechanisms that regulate the expression of GSK genes during plant development and stress responses are still largely unknown. This study provides a detailed analysis of GSK promoters in rice and Arabidopsis, as well as characterization of expression profiles under different conditions. The results offer valuable insights into the regulatory mechanisms and may serve as a reference for future research in other plant species.
Editorial Material
Biochemistry & Molecular Biology
Sunny Ahmar, Damian Gruszka
Summary: This study reports a new strategy to create high-yielding, semi-dwarf wheat varieties with improved nitrogen-use efficiency by inhibiting brassinosteroid signaling through CRISPR/Cas9-mediated knockout of the ZnF-B gene encoding a zinc-finger RING-type E3 ligase.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Katarzyna Nowak, Barbara Wojcikowska, Monika Gajecka, Anna Elzbieciak, Joanna Moronczyk, Anna M. Wojcik, Przemyslaw Zemla, Sylvie Citerne, Agnieszka Kiwior-Wesolowska, Justyna Zbieszczyk, Malgorzata D. Gaj
Summary: Genotype-limited plant regeneration in barley can be improved by treating explants with chromatin modifiers like TSA.
JOURNAL OF APPLIED GENETICS
(2023)
Review
Biochemistry & Molecular Biology
Marzena Kurowska, Agata Daszkowska-Golec
Summary: NAC family gene - SNAC1 plays a crucial role in plant adaptation to abiotic stress such as drought and salinity. It functions as a regulator by controlling the expression of downstream genes, thus enhancing stress tolerance. However, the detailed mechanism of SNAC1 in molecular and physiological responses still requires further investigation.
Review
Biotechnology & Applied Microbiology
Sunny Ahmar, Goetz Hensel, Damian Gruszka
Summary: Cereal crops play a significant role in human consumption and animal feed, but face challenges from climate change and population expansion. Gene-editing technologies, such as CRISPR/Cas9, offer a potential solution to develop stress-tolerant and high-yielding cereal varieties. However, there are challenges to overcome, including time-consuming processes, limited genotypes for transformation, and restrictive regulations on genetically modified organisms (GMOs).
BIOTECHNOLOGY ADVANCES
(2023)