Article
Microbiology
Xi Guo, Jing Wang, Pan Wu, Shujie Li, Lu Feng, Bin Liu, Lei Wang
Summary: A molecular serotyping scheme for Morganella morganii was developed based on genetic variation, along with a traditional serotyping scheme that was shown to be consistent. Additionally, a microsphere-based suspension array with high specificity was introduced. This work provides an important tool for the detection and epidemiological surveillance of M. morganii, with potential wide applicability.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Pierre Lopez, Sebastien Bridel, Denis Saulnier, Rarahu David, Beatriz Magarinos, Beatriz S. Torres, Jean Francois Bernardet, Eric Duchaud
Summary: This study identified the genomic loci involved in the O-antigen biosynthesis of Tenacibaculum maritimum, a devastating bacterial pathogen affecting marine fish. By combining conventional serotyping and genome-wide association study, a robust multiplex PCR-based serotyping scheme was developed, outperforming traditional serotyping. This method could be useful for population structure analysis, disease surveillance, vaccine formulation, and efficacy follow-up.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Parasitology
Elahe Tajbakhsh, Shima Shantiae, Hassan Momtaz
Summary: This study investigated the mechanisms of resistance in Enterobacter aerogenes strains isolated from the urinary tract of infected patients. The findings indicate that E. aerogenes strains exhibit high levels of antibiotic resistance and biofilm formation, and are associated with various resistance and virulence genes.
Article
Biochemistry & Molecular Biology
Andrei Perepelov, Andrei Filatov, Wenxuan Zhu, Alexander S. Shashkov, Min Wang, Xi Guo
Summary: The study isolated and studied the O-polysaccharide of Enterobacter cloacae G3422 using chemical methods, established the structure of the repeating unit, and sequenced the O-antigen gene cluster, finding good agreement between gene functions and O-antigen structure.
CARBOHYDRATE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Andrei V. Filatov, Andrei V. Perepelov, Alexander S. Shashkov, Gennady L. Burygin, Natalia E. Gogoleva, Yuriy A. Khlopko, Vyacheslav S. Grinev
Summary: This study investigated the chemical structure and gene cluster of Enterobacter cloacae K7 O-antigen, revealing a tetrasaccharide O-unit structure and identifying most genes necessary for O-antigen synthesis within the gene cluster.
CARBOHYDRATE RESEARCH
(2021)
Article
Medicine, Research & Experimental
Yoshihiro Shioi, Mitsumasa Osakabe, Naoki Yanagawa, Hiroyuki Nitta, Akira Sasaki, Tamotsu Sugai
Summary: Comprehensive genomic profiling is important in evaluating the carcinogenesis of biliary tract carcinoma, with gall bladder carcinoma and biliary duct carcinoma showing different genetic backgrounds in terms of somatic copy number alterations (SCNAs). These findings could aid in understanding the molecular carcinogenesis of biliary tract carcinomas.
Article
Immunology
Min Wang, Lu Wang, Pan Wu, Tingting Chen, Yiming Zhu, Yang Zhang, Yi Wei, Chengqian Qian, Yuhui Wang, Bin Liu
JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Immunology
Bin Liu, Junyue Wang, Lu Wang, Peng Ding, Pan Yang, Bin Yang
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Cell Biology
Lingyan Jiang, Peisheng Wang, Xiaomin Li, Runxia Lv, Lin Wang, Bin Yang, Di Huang, Lu Feng, Bin Liu
CELLULAR MICROBIOLOGY
(2020)
Review
Microbiology
Bin Liu, Axel Furevi, Andrei Perepelov, Xi Guo, Hengchun Cao, Quan Wang, Peter R. Reeves, Yuriy A. Knirel, Lei Wang, Goran Widmalm
FEMS MICROBIOLOGY REVIEWS
(2020)
Article
Microbiology
Tianyuan Jia, Bin Liu, Huiqian Mu, Chengqian Qian, Lu Wang, Linxing Li, Gege Lu, Wenxuan Zhu, Xi Guo, Bin Yang, Di Huang, Lu Feng
Summary: The study introduces a novel sRNA, EsrF, in Enterohemorrhagic Escherichia coli O157:H7, which senses high ammonium concentrations in the colon and promotes pathogenicity by enhancing bacterial motility and adhesion to host cells. The findings demonstrate that EsrF interacts with the flagellar biosynthetic gene, flhB, mRNA and upregulates the expression of flagellar genes, leading to increased motility and virulence. Additionally, high ammonium concentrations in the colon induce EsrF expression, releasing the inhibition by NtrC and promoting bacterial colonization in the host colon.
Article
Multidisciplinary Sciences
Yutao Liu, Bin Liu, Tingting Xu, Qian Wang, Wendi Li, Jialin Wu, Xiaoyu Zheng, Bin Liu, Ruiying Liu, Xingmei Liu, Xi Guo, Lu Feng, Lei Wang
Summary: The study identifies a fructose uptake system crucial for the growth of Vibrio cholerae in seawater, where fructose concentrations are low. This system may be important for the survival of pandemic V. cholerae in seawater.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Di Huang, Yuhui Wang, Jing Zhang, Huimin Xu, Jing Bai, Huijing Zhang, Xiaolong Jiang, Jian Yuan, Gege Lu, Lingyan Jiang, Xiaoping Liao, Bin Liu, Huanhuan Liu
Summary: In this study, it was found that nitrogen, particularly in the form of ammonium, plays a critical role in promoting Monascus pigment biosynthesis diversity. The production of red, orange, and yellow pigments is regulated differently, with gene clusters showing low contribution to pigment accumulation. Red pigment biosynthesis is closely associated with intracellular amino acids, while orange and yellow pigments are linked to nucleotides, demonstrating a combined regulation of pigment diversity at metabolomic and transcriptomic levels.
Article
Food Science & Technology
Lu Wang, Wenxuan Zhu, Gege Lu, Pan Wu, Yi Wei, Yingying Su, Tianyuan Jia, Linxing Li, Xi Guo, Min Huang, Qian Yang, Di Huang, Bin Liu
Summary: Traditional serotyping methods for foodborne pathogen Cronobacter are time-consuming and inefficient, but the newly developed CroTrait tool enables rapid and accurate species identification and O serotyping based on whole-genome sequencing data. Additionally, it has the capability to predict new potential O serotypes, making it a convenient and promising tool for Cronobacter isolates.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Microbiology
Bin Liu, Chengqian Qian, Pan Wu, Xiaodan Li, Yutao Liu, Huiqian Mu, Min Huang, Yang Zhang, Tianyuan Jia, Yuanyuan Wang, Lu Wang, Xiao Zhang, Di Huang, Bin Yang, Lu Feng, Lei Wang
Summary: Enterohemorrhagic Escherichia coli regulates its cell wall structure during infection, balancing survival in the intestinal lumen and infection of intestinal epithelial cells. The bacteria express long-chain OAg in the intestinal lumen for increased resistance to antimicrobial peptides, but reduce OAg chain length upon sensing epithelial cell attachment, leading to enhanced injection of T3SS effectors into epithelial cells for host cell infection. This regulatory mechanism may be widespread in pathogenic bacteria and closely related to bacterial pathogenesis.
Article
Microbiology
Xi Guo, Jing Wang, Pan Wu, Shujie Li, Lu Feng, Bin Liu, Lei Wang
Summary: A molecular serotyping scheme for Morganella morganii was developed based on genetic variation, along with a traditional serotyping scheme that was shown to be consistent. Additionally, a microsphere-based suspension array with high specificity was introduced. This work provides an important tool for the detection and epidemiological surveillance of M. morganii, with potential wide applicability.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Bin Liu, Yutao Liu, Bin Yang, Qian Wang, Xingmei Liu, Jingliang Qin, Ke Zhao, Fan Li, Xiaohui Feng, Linxing Li, Pan Wu, Miaomiao Liu, Siwei Zhu, Lu Feng, Lei Wang
Summary: This study reveals that EHEC O157:H7 acquired a previously uncharacterized regulatory system rbfSR, which senses riboflavin produced by gut microbiota and activates the expression of virulence genes. This acquisition represents an essential step in the evolution of the highly pathogenic O157:H7. The introduction of rbfSR into other EHEC serotypes also increased their pathogenicity, indicating a potential public health issue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Tianyuan Jia, Pan Wu, Bin Liu, Miaomiao Liu, Huiqian Mu, Dan Liu, Min Huang, Linxing Li, Yi Wei, Lu Wang, Qian Yang, Yutao Liu, Bin Yang, Di Huang, Liang Yang
Summary: Escherichia coli strains including enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) can cause severe gastroenteritis. An RNA called EsrL promotes the pathogenicity, adhesion, and biofilm formation of these strains. The expression of EsrL is repressed by PhoB under low-phosphate conditions, but relieved in a phosphate-rich environment. EsrL interacts with and stabilizes important transcripts, leading to increased adhesion and colonization in the host intestine.
Article
Gastroenterology & Hepatology
Jialin Wu, Yutao Liu, Wendi Li, Fan Li, Ruiying Liu, Hao Sun, Jingliang Qin, Xiaohui Feng, Di Huang, Bin Liu
Summary: This study systematically investigates the influence of MerR family regulators on intestinal colonization of V. cholerae. The researchers found that MlrA significantly promotes the colonization capacity of V. cholerae in the host intestine by directly enhancing the expression of the important virulence factor tcpA. Furthermore, they revealed that mlrA is activated by anaerobic signals in the small intestine of the host through Fnr during infection. These findings provide insights into a MlrA-mediated virulence regulation pathway in V. cholerae, allowing it to sense environmental signals and activate virulence gene expression.
Article
Chemistry, Multidisciplinary
Jian Yuan, Xiaolong Jiang, Huijing Zhang, Jing Bai, Jing Zhang, Yuhui Wang, Gege Lu, Huimin Xu, Huanhuan Liu, Bin Liu, Di Huang
Summary: This study developed a green artificial biosynthesis pathway for catalyzing the poly-LacNAc glycosylation of protein in the cytoplasm of Escherichia coli, utilizing enzymes from Streptococcus parasanguinis, Neisseria meningitidis, and Helicobacter pylori. By rewiring the metabolic network of the strain, the researchers achieved the goal of poly-LacNAc glycoprotein biosynthesis and further improved the degree of polymerization. This approach opens up new avenues for efficient and green manufacturing of various glycoprotein structures with diverse biomedical applications, reducing or eliminating the use or generation of hazardous substances.
Article
Immunology
Yutao Liu, Bin Liu, Pan Yang, Ting Wang, Zhanhe Chang, Junyue Wang, Qian Wang, Wendi Li, Jialin Wu, Di Huang, Lingyan Jiang, Bin Yang