Article
Multidisciplinary Sciences
Tsz-Yin Chan, Christina M. Egbert, Julia E. Maxson, Adam Siddiqui, Logan J. Larsen, Kristina Kohler, Eranga Roshan Balasooriya, Katie L. Pennington, Tsz-Ming Tsang, Madison Frey, Erik J. Soderblom, Huimin Geng, Markus Muschen, Tetyana V. Forostyan, Savannah Free, Gaelle Mercenne, Courtney J. Banks, Jonard Valdoz, Clifford J. Whatcott, Jason M. Foulks, David J. Bearss, Thomas O'Hare, David C. S. Huang, Kenneth A. Christensen, James Moody, Steven L. Warner, Jeffrey W. Tyner, Joshua L. Andersen
Summary: TNK1, a non-receptor tyrosine kinase, has been found to play a key role in cancer by toggling between inactive states involving interaction with 14-3-3 and active states through ubiquitin binding. The discovery of a TNK1 inhibitor, TP-5801, highlights its potential as a therapeutic target for TNK1-transformed cells and tumor growth suppression in vivo.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Subashini Mathivanan, Puneeth Kumar Chunchagatta Lakshman, Manvi Singh, Saranya Giridharan, Keerthana Sathish, A. Manjunath Hurakadli, Kavitha Bharatham, Neelagandan Kamariah
Summary: In this study, the interaction between FOXO3apS253 phosphopeptide and 14-3-36 was comprehensively analyzed using fluorescence polarization, isothermal titration, and molecular dynamics simulations. The high-resolution structure of the FOXO3apS253:14-3-36 complex revealed a distinct mode of interaction compared to other 14-3-3 phosphopeptide complexes. The study also identified structural differences between the binding of FOXO1pS256 and FOXO3apS253 to various 14-3-3 isoforms, providing a framework for the rational design of isoform-specific FOXO/14-3-3 protein-protein interaction inhibitors for therapy.
Review
Pharmacology & Pharmacy
Sathapana Kongsamut, Haifeng Eishingdrelo
Summary: The activation of G-protein-coupled receptors (GPCRs) triggers a cascade of reactions, including changes in receptor structures, phosphorylation, protein recruitment, trafficking and gene expression. Two well-studied signaling pathways of GPCRs are G-protein-mediated and arrestin-mediated pathways. Recently, interactions between GPCRs and 14-3-3 proteins have been discovered, which opens new possibilities in signal transduction. 14-3-3 proteins are crucial for GPCR trafficking and signal transduction, providing potential for studying GPCR function and therapeutics.
DRUG DISCOVERY TODAY
(2023)
Article
Biology
Francois Enchery, Claire Dumont, Mathieu Iampietro, Rodolphe Pelissier, Noemie Aurine, Louis-Marie Bloyet, Caroline Carbonnelle, Cyrille Mathieu, Chloe Journo, Denis Gerlier, Branka Horvat
Summary: The highly pathogenic bat-borne Nipah virus (NiV) inhibits the NF-kappa B pathway via its nonstructural W protein by causing nuclear accumulation of the cellular scaffold protein 14-3-3, shedding light on the pathomechanism of NiV infection.
COMMUNICATIONS BIOLOGY
(2021)
Article
Plant Sciences
Pengyu Song, Zidan Yang, Can Guo, Run Han, Huaichang Wang, Jie Dong, Dingming Kang, Yan Guo, Shuhua Yang, Jigang Li
Summary: In this study, it was found that 14-3-3 lambda and kappa are important positive regulators of photomorphogenesis in plants. They bind to PIF3 and promote the formation of the phyB-PIF3-PPK complex, thereby regulating the light signaling pathway in Arabidopsis.
Article
Biochemistry & Molecular Biology
Junwon Kim, Mark D. Rose
Summary: In Saccharomyces cerevisiae, the p21-activated kinase Cla4p regulates polarized morphogenesis and cytokinesis. Cla4p kinase activity is down-regulated during mating, and this is achieved through phosphorylation of Cla4p at multiple sites, including Cla4p-S186, which reduces its kinase activity. Fus3p counteracts Cla4p kinase activity during mating by phosphorylating Fus2p at different sites to promote nuclear export and block import.
Article
Multidisciplinary Sciences
Lea Reuter, Tanja Schmidt, Prabha Manishankar, Christian Throm, Jutta Keicher, Andrea Bock, Irina Droste-Borel, Claudia Oecking
Summary: NPH3 plays a crucial role in auxin-dependent plant phototropism, with blue light triggering its dissociation from the plasma membrane through phosphorylation and interaction with 14-3-3 proteins. In darkness, NPH3 binds polyacidic phospholipids for membrane association. The dynamic change in NPH3 localization mediated by 14-3-3 is essential for auxin-dependent phototropism.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yuval Yung, Zhong Yao, Tamar Hanoch, Rony Seger
Summary: This study identified a new splice variant of ERK, termed ERK1b, with a molecular weight of 46kDa. ERK1b was found to be the major isoform responding to exogenous stimulation in Ras-transformed cells, possibly due to its differential regulation by MAPK/ERK kinase and phosphatases.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Xiaojing Dong, Feng Feng, Yangjun Li, Lin Li, She Chen, Jian-Min Zhou
Summary: Specific 14-3-3 proteins are crucial for the activation of MAPK cascades initiated by surface immune receptors. The phosphorylation of MAPKKKs and subsequent MAP kinase activation are dependent on the lambda and kappa isoforms of 14-3-3 proteins, which interact with RLCKs and MAPKKKs. The interaction of 14-3-3 proteins with MAPKKK5 promotes the phosphorylation of MAPKK5 and enhances plant disease resistance.
Article
Endocrinology & Metabolism
Sabri A. Rial, Rahaf Shishani, Bethany P. Cummings, Gareth E. Lim
Summary: Since their discovery, 14-3-3 protein family has been recognized as pleiotropic regulators of diverse cellular and physiological functions. They play important roles in metabolism, pancreatic beta-cell biology, and diabetes. This article discusses the regulatory functions of 14-3-3 proteins and their potential as targets for treating metabolic diseases like diabetes.
Review
Biochemistry & Molecular Biology
Veronika Obsilova, Tomas Obsil
Summary: Signal transduction cascades efficiently transmit chemical and/or physical signals from the extracellular environment to intracellular compartments, thereby eliciting an appropriate cellular response. 14-3-3 proteins, as a family of highly conserved scaffolding molecules, play a crucial role in modulating the function of other proteins primarily through phosphorylation-dependent mechanisms. They participate in key cellular processes such as cell-cycle control, apoptosis, signal transduction, energy metabolism, and protein trafficking. However, our understanding of the molecular mechanisms by which 14-3-3 proteins regulate their binding partners remains insufficient, despite intensive research into their protein-protein interactions. This review article aims to provide an overview of recent structural studies of 14-3-3 protein complexes in order to further explore the regulatory mechanisms of these proteins.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Plant Sciences
Hongbo Li, Juan Wei, Yanglan Liao, Xiaoling Cheng, Shuhong Yang, Xiaohong Zhuang, Zhonghui Zhang, Wenjin Shen, Caiji Gao
Summary: This study reveals a previously unidentified function of plant-specific casein kinase I members in attenuating ABA signaling by regulating the phosphorylation and nuclear accumulation of FREE1.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Stuart Sullivan, Thomas Waksman, Louise Henderson, Dimitra Paliogianni, Melanie Luetkemeyer, Noriyuki Suetsugu, John M. Christie
Summary: The phosphorylation of NPH3 by phototropin 1 is essential for phototropic growth in Arabidopsis, triggering binding of 14-3-3 proteins to NPH3 for the phototropic response.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jakub Masaryk, Deepika Kale, Pavel Pohl, Francisco J. Ruiz-Castilla, Olga Zimmermannova, Veronika Obsilova, Jose Ramos, Hana Sychrova
Summary: Potassium is crucial for cellular processes, and its uptake and efflux through the plasma membrane need to be precisely regulated. The intracellular hydrophilic loop (IL2) of the Trk1 protein plays a key role in its targeting and activity regulation, and phosphorylation of specific residues within IL2 is important for these processes.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Jun Hu, Xue-Meng Sun, Jing-Yun Su, Yu-Fen Zhao, Yong-Xiang Chen
Summary: The study explores the effects and molecular mechanisms of protein posttranslational modifications on protein-protein interactions, revealing that phosphorylated residues and farnesylated residues have different enhancing effects on protein interactions. Different PTM patterns are proposed to affect the binding process through distinct mechanisms.
Article
Biochemistry & Molecular Biology
Huihui Liu, Chuanwei Yang, Lin Li
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2016)
Article
Plant Sciences
L. Ma, X. Huang, R. Yu, X. L. Jing, J. Xu, C. A. Wu, C. X. Zhu, H. M. Liu
Article
Plant Sciences
Maolin Peng, Zepeng Li, Nana Zhou, Mengmeng Ma, Yupei Jiang, Aiwu Dong, Wen-Hui Shen, Lin Li
Review
Plant Sciences
Chuanwei Yang, Lin Li
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Cell Biology
Chuanwei Yang, Famin Xie, Yupei Jiang, Zhen Li, Xu Huang, Lin Li
DEVELOPMENTAL CELL
(2018)
Article
Plant Sciences
Xu Huang, Ru Yu, Wenjing Li, Liwei Geng, Xiuli Jing, Changxiang Zhu, Hongmei Liu
Article
Biochemistry & Molecular Biology
Renshan Zhang, Chuanwei Yang, Yupei Jiang, Lin Li
Article
Multidisciplinary Sciences
Chuanwei Yang, Liufan Yin, Famin Xie, Mengmeng Ma, Sha Huang, Yue Zeng, Wen-Hui Shen, Aiwu Dong, Lin Li
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cell Biology
Chuanwei Yang, Sha Huang, Yue Zeng, Chang Liu, Qinyi Ma, Jose Pruneda-Paz, Steve A. Kay, Lin Li
Summary: The bHLH transcription factors bHLH48 and bHLH60 have been identified as essential partners for PIF7 in regulating hypocotyl elongation, showing DNA binding activity and enhancing PIF7's ability to bind DNA. They act as positive partners of PIF7 in promoting hypocotyl elongation through mutual benefit.
Article
Plant Sciences
Yue Zeng, Jiayu Wang, Sha Huang, Yu Xie, Tongdan Zhu, Leyi Liu, Lin Li
Summary: Light and temperature are key environmental signals that regulate plant growth. HSP90s play a role in skoto- and thermomorphogenesis by interacting with ELF3 and regulating the function of PIF4. This study highlights the involvement of HSP90s in the signal pathway of light and temperature.
Article
Biochemistry & Molecular Biology
Chuanwei Yang, Tongdan Zhu, Nana Zhou, Sha Huang, Yue Zeng, Wen Jiang, Yu Xie, Wen-Hui Shen, Lin Li
Summary: For shade-intolerant plants, changes in light quality due to competition from neighbors trigger shade avoidance syndrome (SAS), which negatively affects plant health and crop yield. The transcriptional regulator PIF7 interacts with ASF1 and HIRA to promote H3.3 incorporation into chromatin and enhance gene transcription, enabling plants to effectively respond to environmental shade.
Article
Biochemistry & Molecular Biology
Tongdan Zhu, Chuanwei Yang, Yu Xie, Sha Huang, Lin Li
Summary: Shade avoidance syndrome (SAS) triggers various morphological and physiological changes in plants to adapt to limited light conditions. In this study, we identified 211 shade-regulated lncRNAs in Arabidopsis and characterized a specific lncRNA called PUAR. PUAR is induced by shade and promotes hypocotyl elongation. It functions by interacting with PIF7 and inhibiting the induction of PHYA, thereby regulating SAS.
Article
Plant Sciences
Xu Huang, Hao Tian, Jeongmoo Park, Dong-Ha Oh, Jianhong Hu, Rodolfo Zentella, Hong Qiao, Maheshi Dassanayake, Tai-Ping Sun
Summary: DELLA genes encode conserved master growth regulators that control plant development in response to internal and environmental cues. This study reveals that the PFYRE subdomain of DELLA interacts with histone H2A to form a TF-DELLA-H2A complex, which stabilizes on the target chromatin. These findings provide insights into the modular functions of DELLA in genome-wide transcription regulation in plants.