Article
Agriculture, Multidisciplinary
Long Ke-ren, Li Xiao-kai, Zhang Ruo-wei, Gu Yi-ren, Du Min-jie, Xing Xiang-yang, Du Jia-xiang, Mai Miao-miao, Wang Jing, Jin Long, Tang Qian-zi, Hu Si-lu, Ma Ji-deng, Wang Xun, Pan Deng-ke, Li Ming-zhou
Summary: Follistatin (FST) is an important regulator of skeletal muscle growth and adipose deposition through its ability to bind to members of the TGF-beta superfamily. This study generated transgenic pigs that express human FST specifically in skeletal muscle tissues and found that these pigs exhibited increased muscle growth, decreased adipose deposition, and improved metabolism. Transcriptome analysis revealed the involvement of various signaling pathways and metabolic processes in the phenotypic changes observed. These findings provide new insights into the molecular mechanisms underlying the effects of FST in pigs.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Agriculture, Dairy & Animal Science
Vetriselvi Sampath, Dong Heon Baek, Sureshkumar Shanmugam, In Ho Kim
Summary: The experiment showed that supplementing piglets with blood plasma and yeast significantly improved growth performance, nutrient digestibility, fecal microbial, and reduced harmful gas emissions. This suggests that the dietary supplement with blood plasma and yeast could serve as an excellent alternative to antibiotics growth promoters in the diet of weaned piglets.
Article
Agriculture, Dairy & Animal Science
Santi D. Upadhaya, Sang Seon Lee, Young Hwa Kim, Zhenlong Wu, In Ho Kim
Summary: This study evaluated the impact of supplementing glutamic acid to reduced protein diets on the growth performance of weanling pigs. The results showed that supplementing glutamic acid enhanced the body weight, average daily gain, and gain-to-feed ratio of the weanling pigs. The study also observed a linear increase in these growth parameters with increasing levels of glutamic acid. Additionally, the supplementation of glutamic acid had an effect on ammonia and methyl mercaptans emissions.
Article
Fisheries
Yu Zhang, Zeyuan Tang, Zhifei Liu, Jingyi Shen, Huijie Feng, Liangyi Xue
Summary: The inhibition of MSTN1 in transgenic red carp promoted muscle growth without affecting muscle fat content, which differs from mammals. The study found that the expression of MSTN1 propeptide and growth-related genes were significantly higher in transgenic red carp, indicating muscle hypertrophy.
Article
Ecology
Thomas Kopp, Markus Nabernegg
Summary: The study provides empirical evidence on the environmental impact of inequality and suggests that reducing inequality levels can increase carbon footprints. There is a conflict of objectives in relatively rich and egalitarian countries for surface sealing and forest land use, while relatively poor and initially unequal countries have room for jointly reducing environmental impacts and inequality. The results highlight the importance of accompanying policy measures to reduce inequality with policies that limit harmful environmental impacts in order to achieve joint reductions in inequality and environmental impact, especially in wealthier countries.
ECOLOGICAL ECONOMICS
(2022)
Article
Multidisciplinary Sciences
Beate Rieblinger, Hicham Sid, Denise Duda, Tarik Bozoglu, Romina Klinger, Antonina Schlickenrieder, Kamila Lengyel, Krzysztof Flisikowski, Tatiana Flisikowska, Nina Simm, Alessandro Grodziecki, Carolin Perleberg, Andrea Bahr, Lucie Carrier, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Eckhard Wolf, Lutz Kettler, Harald Luksch, Ibrahim T. Hagag, Daniel Wise, Jim Kaufman, Benedikt B. Kaufer, Christian Kupatt, Angelika Schnieke, Benjamin Schusser
Summary: Research on genetically modified animals has mostly focused on mice, but also includes species like pigs that are more physiologically similar to humans, and cross-species comparisons with phylogenetically distant species like chickens. CRISPR-Cas9 is a versatile genetic editing method applicable across various species. The successful generation of transgenic chickens and pigs that express Cas9 in all organs has confirmed the functionality of Cas9 across different target genes, cell types, and in vivo applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Agriculture, Dairy & Animal Science
Shanchuan Cao, Wenjie Tang, Hui Diao, Shuwei Li, Honglin Yan, Jingbo Liu
Summary: This study examined the effects of feeding frequency on growth performance, nutrient digestibility, carcass quality, and lipid metabolism in growing-finishing pigs. The results showed that reducing feeding frequency improved feed conversion ratio and had an impact on fat deposition and carcass composition. This study provides a new approach to feeding and management of growing-finishing pigs.
Article
Biotechnology & Applied Microbiology
Renato Nunes de Lima Seixas, Jose Maria Ferreira Jardim da Silveira, Vinicius Eduardo Ferrari
Summary: GM seeds have had significant impacts on global agriculture, particularly in relation to pest and weed control systems. The study shows that insect resistant traits reduce insecticide use and environmental impact, while herbicide tolerant traits increase herbicide use and environmental impact.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yubo Qing, Muhammad Ameen Jamal, Dejia Shi, Sumei Zhao, Kaixiang Xu, Deling Jiao, Heng Zhao, Honghui Li, Baoyu Jia, Haizhen Wang, Hong-Ye Zhao, Hong-Jiang Wei
Summary: Leptin overexpressing transgenic pigs exhibited elevated levels of leptin protein and mRNA expression, leading to impaired growth, decreased subcutaneous fat, altered lipid metabolism, and dysregulation of lipid metabolism related genes at different fat depots and adipocytes levels. These findings suggest that leptin overexpression can disrupt the balance between lipolysis and lipogenesis, resulting in decreased subcutaneous fat depots and potential modeling for obesity and related metabolic disorders.
TRANSGENIC RESEARCH
(2022)
Article
Polymer Science
Guillaume Noirbent, Frederic Dumur
Summary: The development of light-assisted polymerization, especially visible light photopolymerization, aims to reduce environmental impact. However, toxicity of current visible light photoinitiators remains a short-term challenge, prompting the search for natural products as promising alternatives.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Engineering, Mechanical
Alban Robin, Peter Davies, Mael Arhant, Sebastian Le Jeune, Nicolas Lacotte, Eliaz Morineau, Fanny Ioos, Pascal Dourlen, Raphael Cairo
Summary: This study evaluates the performance of alternative sandwich materials with reduced environmental impact under loading conditions relevant to boat design. Compared to traditional PVC foam sandwich materials, flax and bamboo composite faced PET foam sandwich materials have lower flexural stiffness and damage initiation levels, but they show promising support for impact energy levels that could be further improved with facing enhancements.
JOURNAL OF SANDWICH STRUCTURES & MATERIALS
(2023)
Article
Anatomy & Morphology
Thankam S. Nair, Bala Naveen Kakaraparthi, Lucy Yang, Lillian Lu, Trey B. Thomas, Anna C. Morris, Pavan Kommareddi, Ariane Kanicki, Thomas E. Carey
Summary: The presence of Slc44a2 is associated with the expression and localization of cell adhesion proteins, and cells with Slc44a2 knockout demonstrate lower adhesion and faster proliferation. Additionally, there are significant alterations in the distribution of CD9 and CD81 in KO cells.
Article
Chemistry, Inorganic & Nuclear
Guoping Liu, Nan Wang, Fangya Qi, Xiaoyi Lu, Yaohua Liang, Zhipeng Sun
Summary: This study introduces Ge into the Ni-P system to synthesize Ni-Ge-P composites as anodes for lithium-ion batteries. The results show that the Ge-substituted Ni-Ge-P composites exhibit improved electrochemical reversibility and reduced redox potential compared to Ni-P anodes. The Li-storage mechanism of a selected NiGe0.5P0.5/Ni2Ge0.5P0.5 composite was investigated and a topological conversion reaction mechanism was proposed.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Multidisciplinary
Liyu Zhu, Yucheng Li, Jingyang Zhao, Jing Liu, Jiandu Lei, Luying Wang, Chaobo Huang
Summary: The use of lignosulfonate acid (LSA) as an additive in Nafion membranes for fuel cell applications enhances thermal stability and other properties, making their use in PEMFCs attractive and cost-effective.
CHEMICAL COMMUNICATIONS
(2021)
Article
Agriculture, Dairy & Animal Science
J. C. Lynegaard, N. J. Kjeldsen, J. K. Bache, N. R. Weber, C. F. Hansen, J. P. Nielsen, C. Amdi
Summary: This study investigated four different dietary protein strategies to prevent diarrhea in pigs when medicinal zinc oxide is phased out. The results suggested that zinc oxide has a positive effect on non-infectious diarrhea, but can affect pig growth performance.
Article
Biochemistry & Molecular Biology
Guoling Li, Haoqiang Wang, Xianwei Zhang, Zhenfang Wu, Huaqiang Yang
Summary: This study presents a strategy to enhance HDR efficiency in animal cells by spatial and temporal colocalization of the donor and Cas9 through coupling the CRISPR system with the transcription factor THAP11. By appending THAP11-specific DNA binding motifs to the double-stranded donor DNA, the HDR efficiency can be significantly improved. The THAP11-fused CRISPR system achieved a more than twofold increase in HDR-mediated knock-in efficiency, and up to 6-fold increase can be achieved through combinational use with the small-molecule HDR enhancer valnemulin.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Chuanzhao Yu, Haiwen Zhong, Xiaohui Yang, Guoling Li, Zhenfang Wu, Huaqiang Yang
Summary: The study established a pig genome-scale CRISPR/Cas9 knockout library targeting 16,888 genes, which was used to screen pig cells for genes critical for toxin toxicity. The identified candidates were confirmed through gene knockout and toxicity assays, suggesting the library as a promising platform for pig functional genomic analysis.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Agriculture, Dairy & Animal Science
Xin Zhao, Weican Wan, Bin Li, Xianyu Zhang, Mao Zhang, Zhenfang Wu, Huaqiang Yang
Summary: Spermatogonial stem cells (SSCs) are adult stem cells capable of passing genetic information to offspring through self-renewal and differentiation into mature spermatozoa. Optimal in vitro culture of SSCs can benefit various studies and applications related to SSC biology.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Cell & Tissue Engineering
Ming Jin, Danni Wang, Guoling Li, Jiajia Lin, Xiang Lin, Ning Wang
Summary: In this study, an induced pluripotent stem cell line was generated from a DMD patient's dermal fibroblasts using non-integrating Sendai virus. This iPSC line exhibited normal iPSC characteristics and could differentiate into the three germ layers.
STEM CELL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Jianyu Gan, Ting Gu, Huaqiang Yang, Zheng Ao, Gengyuan Cai, Linjun Hong, Zhenfang Wu
Summary: This review discusses the function and mechanism of micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in regulating spontaneous abortion (SA). It suggests the existence of a competitive endogenous RNA (ceRNA) regulatory network in the onset and development of SA. Understanding this network is important for the search of potential RNA markers for SA diagnosis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Xiaohui Yang, Chuanzhao Yu, Qiuyan Zhang, Linjun Hong, Ting Gu, Enqin Zheng, Zheng Xu, Zicong Li, Changxu Song, Gengyuan Cai, Zhenfang Wu, Huaqiang Yang
Summary: This study generated mutant mice with resistance to pseudorabies virus (PRV) by introducing a single amino acid substitution. The mutant mice showed alleviated disease manifestations, decreased death rate, and reduced viral loading in serum and tissue compared to wild-type mice. However, the mutation also caused a defect in eye development.
Article
Biotechnology & Applied Microbiology
Huawei Tong, Jia Huang, Qingquan Xiao, Bingbing He, Xue Dong, Yuanhua Liu, Xiali Yang, Dingyi Han, Zikang Wang, Wenqin Ying, Runze Zhang, Yu Wei, Xuchen Wang, Chunlong Xu, Yingsi Zhou, Yanfei Li, Minqing Cai, Qifang Wang, Mingxing Xue, Guoling Li, Kailun Fang, Hainan Zhang, Hui Yang
Summary: Several engineered Cas13 variants have been found to have efficient targeted degradation activity with reduced collateral effects in mammalian cells. These variants show no off-target effects and cell growth arrest, making them suitable for targeted RNA degradation in basic research and therapeutic applications.
NATURE BIOTECHNOLOGY
(2022)
Article
Cell Biology
Fang Zeng, Sha Liao, Zhe Kuang, Qingchun Zhu, Hengxi Wei, Junsong Shi, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Stefan Moisyadi, Johann Urschitz, Zicong Li, Zhenfang Wu
Summary: Farm animals' salivary glands have the potential to efficiently produce human therapeutic proteins. This study successfully used transgenic pigs to synthesize and purify biologically active human nerve growth factor (hNGF) from their salivary glands. The method employed the replacement of the endogenous gene to avoid contamination during purification. This research is significant for human health and biomedicine.
Article
Biochemistry & Molecular Biology
Haiwen Zhong, Jian Zhang, Cheng Tan, Junsong Shi, Jie Yang, Gengyuan Cai, Zhenfang Wu, Huaqiang Yang
Summary: In this study, we identified four missense mutations that could affect MC1R protein function for eumelanin synthesis by comparing MC1R gene sequences. Using CRISPR-mediated homologous recombination, we successfully mutated the e allele in Duroc pigs to the dominant E-D1 allele at the MC1R locus, leading to uniform black coat color. The modified pigs carried a heterozygous E-D1/e allele at the MC1R locus and retained the genetic background of Duroc breed. Our work provides a gene editing method for pig coat color manipulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Yanshe Xie, Zhiqian Xu, Changhua Wu, Chen Zhou, Xianwei Zhang, Ting Gu, Jie Yang, Huaqiang Yang, Enqin Zheng, Zheng Xu, Gengyuan Cai, Zicong Li, Dewu Liu, Zhenfang Wu, Linjun Hong
Summary: The study found that miR-21-5p can be absorbed by sperm through seminal plasma-derived EVs, overexpression of miR-21-5p inhibits sperm capacitation and acrosome reaction, and the expression level of VCL protein is lower in capacitated sperm. These results suggest that miR-21-5p can prevent sperm capacitation through VCL inhibition.
Article
Biochemical Research Methods
Shanxin Yang, Tingting Liu, Jianxin Mo, Huaqiang Yang, Haoqiang Wang, Guangyan Huang, Gengyuan Cai, Zhenfang Wu, Xianwei Zhang
Summary: Transgenic pigs expressing three microbial enzymes in their salivary glands were found to effectively reduce phosphorus and nitrogen emissions and improve growth performances. These enzymes showed good adaptability in the gastrointestinal tract and improved phosphorus digestibility in high-fiber diets.
TRANSGENIC RESEARCH
(2023)
Article
Microbiology
Xupeng Zang, Wenjing Wang, Shengchen Gu, Ting Gu, Huaqiang Yang, Enqin Zheng, Zheng Xu, Sixiu Huang, Zicong Li, Gengyuan Cai, Linjun Hong, Zhenfang Wu
Summary: This study reveals the dynamic changes of sow vaginal microbiotas and their roles in pregnancy development. It also provides insights into the microbe-host interactions in the reproductive tract, which may lead to new research and intervention targets for improving pregnancy development through modulating lower reproductive tract microbiota.
Article
Medicine, Research & Experimental
Guoling Li, Ming Jin, Zhifang Li, Qingquan Xiao, Jiajia Lin, Dong Yang, Yuanhua Liu, Xing Wang, Long Xie, Wenqin Ying, Haoqiang Wang, Erwei Zuo, Linyu Shi, Ning Wang, Wanjin Chen, Chunlong Xu, Hui Yang
Summary: In this study, a gene editing technique was used to treat Duchenne muscular dystrophy (DMD) in a mouse model. The results showed that the technique effectively repaired the mutation in the DMD gene, restored expression of the dystrophin protein to normal levels, and improved muscle function. This study provides a new approach for the treatment of DMD and other monogenic diseases.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Guoling Li, Xiaohui Yang, Xinxin Luo, Zhenfang Wu, Huaqiang Yang
Summary: The use of small molecules to synchronize the cell cycle in S and G2/M phases enhances the efficiency of CRISPR/Cas9-mediated homology-directed repair (HDR) in animal cells and embryos. The study reveals the common molecular mechanisms that connect cell cycle progression and HDR activity.
CELL AND BIOSCIENCE
(2023)
