Article
Chemistry, Multidisciplinary
Fabien Jammes, Julien Schmidt, George Coukos, Sebastian J. Maerkl
Summary: We have developed an integrated microfluidic cellular processing unit that can autonomously isolate and analyze the cell-surface dissociation rates, and recover selected cells. By performing high-throughput single-cell experiments, we have characterized the pMHC-TCR interactions on live CD8+ T cells.
Article
Chemistry, Physical
Jingyu Qi, Haibin Zhu, Yijian Li, Xiangyu Guan, Ying He, Guanhua Ren, Qiang Guo, Longqi Liu, Ying Gu, Xuan Dong, Ya Liu
Summary: Cell-cell interaction is an important way for transmitting information and activating the effects of functional cells. Researchers propose a droplet-based microfluidic platform for cell-cell interaction sequencing and screening, which allows for efficient analysis and interpretation of single-cell transcriptomes in cell interactions. This work fills a gap in the current experimental platform for cell-cell interaction research and has significant practical implications in biomedicine.
Article
Biochemical Research Methods
Youngtaek Kim, Jiyoung Song, Younggyun Lee, Sunghyun Cho, Suryong Kim, Seung-Ryeol Lee, Seonghyuk Park, Yongdae Shin, Noo Li Jeon
Summary: The newly designed injection molded microfluidic device addresses the inherent limitations of PDMS-manufactured devices and provides a more convenient way to study single-cell behaviors while saving time. The spatiotemporal dynamics in the device were demonstrated through experiments and computational simulations. This easy-to-use plastic microfluidic device promises to open a new approach for investigating the mechanisms of cell behavior at the single-cell level.
Review
Chemistry, Analytical
Federico Sala, Carlotta Ficorella, Roberto Osellame, Josef A. Kaes, Rebeca Martinez Vazquez
Summary: Understanding cell migration is crucial for unraveling physiological phenomena and predicting pathologies like cancer metastasis. Microfluidic devices have been used to investigate cellular migration response by engineering mechanical and spatial stimuli. This review discusses the existing technologies, such as soft lithography and femtosecond laser micromachining, used in microfluidic cellular migration assays. It highlights the different geometrical configurations employed and envisions future developments in in vitro migration studies under spatial confinement in microfluidic devices.
Article
Biochemical Research Methods
Kunpeng Cai, Shruti Mankar, Taiga Ajiri, Kentaro Shirai, Tasuku Yotoriyama
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.
Article
Chemistry, Physical
Pengfei Zhang, Aniruddha M. Kaushik, Kuangwen Hsieh, Sixuan Li, Shawna Lewis, Kathleen E. Mach, Joseph C. Liao, Karen C. Carroll, Tza-Huei Wang
Summary: The development of a new cascaded droplet microfluidic platform has addressed the scalability constraint in rapid single-cell AST technologies, demonstrating the potential for generating multiple groups of droplets with custom antibiotic conditions within a single device. This platform has shown promising results in producing clinically useful antibiograms with minimum inhibitory concentrations in approximately 90 minutes for the first antibiotic and 2 minutes for each subsequent antibiotic condition. The platform also demonstrated potential clinical utility in testing clinical isolates and urine specimens against commonly used antibiotics, achieving high categorical agreements with laboratory-based results in a significantly shorter timeframe.
Article
Biochemical Research Methods
Robert Dimatteo, Dino Di Carlo
Summary: This study presents a workflow for rapid screening and sorting of individual T cells based on the accumulation of IL-2 secretion in nanoliter droplets encoded back onto the secreting cell's surface. By partitioning cells using droplets and eliminating diffusive crosstalk, this method enables rapid accumulation of signals onto cell surfaces, allowing for high-throughput sorting.
Article
Chemistry, Analytical
Zhenna Chen, Beibei Chen, Man He, Bin Hu
Summary: A negative magnetophoresis focusing microchip was developed and coupled to ICP-MS for single-cell analysis, achieving high sample throughput. Study on uptake behaviors revealed that MCF-7 cells exhibited more remarkable heterogeneity when treated with ZnO NPs, with lower uptake content compared to Zn2+.
ANALYTICAL CHEMISTRY
(2022)
Review
Cell Biology
Marlene Geyer, Karla Queiroz
Summary: PDAC, the most common type of pancreatic cancer, is characterized by early metastasis and often requires chemotherapy treatment. With only 20% of tumors resectable, there is an urgent need for innovative modeling platforms to better understand and treat this complex disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Electrical & Electronic
Junwen Zhu, Qiqian Zhang, Fei Liang, Yongxiang Feng, Wenhui Wang
Summary: The article introduces a novel microfluidic channel for cell rotation, achieving high-throughput cell rotation using local circular streaming generated by ultrasonic wave excited bubble arrays. The device has multiple merits such as high throughput, low cost, simple fabrication procedure, and high compatibility for lab-on-chip integration, holding promise in cell observation, medicine development, and biological detection.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2021)
Article
Chemistry, Analytical
Qingyu Ruan, Jian Yang, Fenxiang Zou, Xiaofeng Chen, Qianqian Zhang, Kaifeng Zhao, Xiaoye Lin, Xi Zeng, Xiyuan Yu, Lingling Wu, Shuichao Lin, Zhi Zhu, Chaoyong Yang
Summary: This study introduces a single-CTC mass spectrometry analysis method based on digital microfluidics, which can efficiently and accurately perform multiplex mutation profiling of individual CTCs, offering new avenues for cancer therapy guidance.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Lu Huang, Yin Chen, Jianhua Zhou
Summary: Heterogeneous cell-cell communications are crucial for regulating biological processes and diseases. Traditional cell co-culture methods provide bulk-averaged results, while single-cell co-culture based on microfluidic platforms can reveal heterogeneous interactions between different cell types at single-cell resolution.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Chemistry, Analytical
Chao Xu, Kun Wang, Peng Huang, Demeng Liu, Yimin Guan
Summary: The isolation of single cells is crucial for the advancement of single cell analysis methods. Traditional techniques have limitations in terms of complexity and throughput. A microfluidic chip has been developed using thermal bubble micropump technology, allowing for efficient single-cell isolation without the need for injection pumps or peristaltic pumps. The method has shown high capture rates for both polystyrene beads and cells, making it a promising approach for single-cell isolation.
Article
Biochemical Research Methods
Aliakbar Ebrahimi, Reza Didarian, Hamed Ghorbanpoor, Fatma Dogan Guzel, Hossein Hashempour, Huseyin Avci
Summary: This study used a microfluidic chip-based mimic of high-performance liquid chromatography (HPLC) strategy to successfully separate cyclotides. The results demonstrate that this method is a rapid, cost-effective, and simple way to separate cyclotides, with potential applications in drug discovery research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Renata Maia, Violeta Carvalho, Rui Lima, Graca Minas, Raquel O. Rodrigues
Summary: This systematic review summarizes the recent progress in the use of microneedles (MNs) in biomedical applications, including drug delivery, biomarker detection, and their potential integration with microfluidic devices. Although MNs have been extensively utilized in lab-on-a-chip platforms, recent studies have also explored their applicability in organ-on-a-chip models. Overall, the presence of MNs in advanced microfluidic devices offers simplified drug delivery, microinjection, and biomarker detection through integrated biosensors, enabling real-time monitoring of various biomarkers in lab- and organ-on-a-chip platforms.
