Article
Nutrition & Dietetics
Xin Xu, Hua-Yong Du, Zuliyaer Talifu, Chun-Jia Zhang, Ze-Hui Li, Wu-Bo Liu, Yi-Xiong Liang, Xu-Luan Xu, Jin-Ming Zhang, De-Gang Yang, Feng Gao, Liang-Jie Du, Yan Yu, Ying-Li Jing, Jian-Jun Li
Summary: The combination of BWSTT and GlyNAC has neuroprotective effects, promotes motor function recovery, and delays skeletal muscle atrophy in rats with SCI. GlyNAC alone improves motor function, but the combination with BWSTT is more effective.
Article
Materials Science, Biomaterials
Hongyu Liu, Yanan Zhao, Jun Tong, Xiaowen Shi, Yun Chen, Yumin Du
Summary: The study demonstrates that a new type of chitosan-based nerve-guide hydrogel conduit fabricated using one-step electrofabrication technology has enhanced mechanical flexibility. The CNHC shows cytocompatibility by promoting cell proliferation and adhesion, and is able to guide axon growth to reach a distal stump, indicating great potential in peripheral nerve regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Cell Biology
Elmira Ahmady, Alexandre Blais, Patrick G. Burgon
Summary: Mlip is a recently discovered protein that interacts directly with chromatin and may function as a transcriptional co-factor. In C2C12 myoblasts, blocking Mlip expression down-regulates myogenic regulatory factors and inhibits myogenic differentiation and myotube formation.
Article
Chemistry, Multidisciplinary
Kim Sarah Koeck, Sahar Salehi, Martin Humenik, Thomas Scheibel
Summary: This study focuses on achieving good mechanical stability of collagen fibers through self-assembly during wet-spinning without the need for additional crosslinkers. The produced collagen fibers show fibril alignment and a unique D-band pattern, and are processed into hierarchical assemblies using textile-engineering techniques. These assemblies serve as excellent substrates for muscle microtissue formation, particularly at the muscle-tendon-junction (MTJ) where myoblasts and fibroblasts are co-cultured to develop an MTJ-model.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Food Science & Technology
Peiyuan Li, Ruilin Xu, Yanfei Shi, Xiaoyang Shi, Xinyuan Zhang, Jinjie Li, Guangning Kou
Summary: This study found that luteolin can increase the proportion of slow myofibers and improve skeletal muscle fitness by activating the FLCN-AMPK-PGC-1α pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biotechnology & Applied Microbiology
Muhammad Maulana Malikul Ikram, Sastia Prama Putri, Eiichiro Fukusaki
Summary: The demand for minimally processed fresh fruit is increasing, but their distribution is limited due to their short shelf life. This study found that using chitosan coating with melezitose can prolong the shelf life of fresh-cut pineapple.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
William Roman, Helena Pinheiro, Mafalda R. Pimentel, Jessica Segales, Luis M. Oliveira, Esther Garcia-Dominguez, Mari Carmen Gomez-Cabrera, Antonio L. Serrano, Edgar R. Gomes, Pura Munoz-Canoves
Summary: The study revealed that localized muscle injuries triggered by exercise activate a myofiber self-repair mechanism that is independent of satellite cells. A signaling cascade involving calcium, Cdc42, and phosphokinase C attracts myonuclei to the damaged site, accelerating sarcomere repair and delivering messenger RNA for cellular reconstruction. Myofiber self-repair is a cell-autonomous protective mechanism, offering an alternative model for understanding muscle architecture restoration in health and disease.
Article
Multidisciplinary Sciences
Atsuki Fukutani, Shiho Kunimatsu, Tadao Isaka
Summary: This study examined the influence of caffeine on cross-bridge dynamics and found that the effect of caffeine on cross-bridge dynamics is negligible. The ergogenic effect of caffeine is primarily achieved through facilitating Ca2+ release.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Orlando Laitano, Gerard P. Robinson, Kevin O. Murray, Christian K. Garcia, Alex J. Mattingly, Deborah Morse, Michelle A. King, John D. Iwaniec, Jamal M. Alzahrani, Thomas L. Clanton
Summary: The study revealed that Myd88 signaling in skeletal muscle plays a significant role in the innate immune inflammatory response to peritoneal sepsis, affecting the regulation of inflammatory cytokines and the cellular composition of the peritoneal fluid.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kosmas Kosmas, Zoe Michael, Aimilia Eirini Papathanasiou, Fotios Spyropoulos, Elio Adib, Ravi Jasuja, Helen Christou
Summary: Pulmonary arterial hypertension (PAH) is a severe disease that requires improved therapies. A study on an animal model shows that PAH can cause skeletal muscle dysfunction, possibly due to a shift in skeletal muscle fiber-type specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Surgery
Zi-Xiang Chen, Zhen Yi, Hai-Bin Lu, Lu-Lu Chen, Xiao-Nan Yang, Zuo-Liang Qi
Summary: This study compared the efficacy of conduits fabricated from fresh muscle versus conduits fabricated from predegenerated muscle for repairing peripheral nerve defects. The results showed that the fresh muscle conduit group had larger myelinated nerve fibers with thicker myelin sheaths, higher sciatic function index, and greater Bcl-2/Bax ratio and Schwann cell survival compared to the predegenerated muscle conduit group.
JOURNAL OF CRANIOFACIAL SURGERY
(2022)
Review
Immunology
Xingyu Wang, Lan Zhou
Summary: Skeletal muscle is crucial for physical activity, energy metabolism, and temperature maintenance. Muscle stem cells, satellite cells, play a vital role in maintaining homeostasis and promoting muscle regeneration. Intramuscular macrophages, functionally diverse, regulate the quiescence, activation, and differentiation of satellite cells. Aberrant activation of intramuscular macrophages, often triggered by altered muscle microenvironment, disrupts muscle homeostasis and regenerative repair. Manipulating the functional activation of macrophages has potential therapeutic implications for muscle diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Hongmei Shi, Jing Fu, Yang He, Zijian Li, Jiajia Kang, Changjie Hu, Xiannian Zi, Yong Liu, Jinbo Zhao, Tengfei Dou, Junjing Jia, Yong Duan, Kun Wang, Changrong Ge
Summary: This study compared the differences in skeletal muscle development between Tengchong snow black meat chickens and white meat chickens, and found that excessive melanin deposition has an effect on skeletal muscle development. The study also validated key candidate genes associated with muscle development and melanin synthesis. The results showed that black meat chickens had inhibited breast and leg muscle development, and melanin deposition was mainly concentrated in the leg muscles, producing an inhibitory effect similar to MSTN.
Article
Polymer Science
Nurjemal A. Tagandurdyyeva, Maxim A. Trube, Igor' O. Shemyakin, Denis N. Solomitskiy, German V. Medvedev, Elena N. Dresvyanina, Yulia A. Nashchekina, Elena M. Ivan'kova, Irina P. Dobrovol'skaya, Almaz M. Kamalov, Elena G. Sukhorukova, Olga A. Moskalyuk, Vladimir E. Yudin
Summary: New tubular conduits consisting of poly (L-lactide) nanofibers and chitosan composite fibers with chitin nanofibrils have been developed for peripheral nerve regeneration and repair. In vitro studies showed good biocompatibility with human embryonic bone marrow stromal cells (FetMSCs), with cell adhesion and differentiation observed just one day after cultivation. An in vivo study evaluating motor-coordination disorders using the sciatic nerve functional index (SFI) assessment demonstrated an increased regeneration rate of the sciatic nerve with the presence of chitosan monofibers and chitosan composite fibers with chitin nanofibrils in the conduit design, resulting in an improved SFI value ranging from 76 to 83.
Article
Engineering, Biomedical
Dahao Wang, Haosen Zhao, Chang Xu, Sen Lin, Yue Guo
Summary: This study demonstrates that in situ 5-ASA grafted chitosan electrospun fibers (ASA-EF) can effectively reduce inflammation and promote nerve regeneration after spinal cord injury. ASA-EF administration leads to the downregulation of inflammatory cytokine expression and the upregulation of anti-inflammatory and regenerative gene expression. It also polarizes macrophages towards proregenerative phenotypes, reduces cavity area, increases myelination and regenerating axons, and improves motor function.
MATERIALS TODAY BIO
(2023)
Review
Orthopedics
Stefano Artiaco, Federico Fusini, Alessandra Colozza, Alessandro Aprato, Alessandro Bistolfi, Alessandro Masse, Bruno Battiston
Summary: Recent studies have found that simple elbow dislocation combined with forearm joint injury is a rare traumatic pattern usually caused by high-energy trauma. A thorough understanding of elbow and forearm biomechanics, and classification of anatomical lesion is essential for successful treatment.
EUROPEAN JOURNAL OF ORTHOPAEDIC SURGERY AND TRAUMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Benedetta Elena Fornasari, Federica Zen, Giulia Nato, Marco Fogli, Federico Luzzati, Giulia Ronchi, Stefania Raimondo, Giovanna Gambarotta
Summary: Angiogenesis plays a key role in nerve conduits by supporting cell survival and providing a pathway for the migration of newly formed Schwann cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Luisa Muratori, Federica Fregnan, Monica Maurina, Kirsten Haastert-Talini, Giulia Ronchi
Summary: Peripheral nerve injuries can lead to loss of motor and sensory function, and current methods for nerve regeneration have limited success. A potential approach for enhancing peripheral nerve regeneration is through improving diet and lifestyle, and consuming polyphenols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Giacomo Carta, Benedetta Elena Fornasari, Federica Fregnan, Giulia Ronchi, Stefano De Zanet, Luisa Muratori, Giulia Nato, Marco Fogli, Giovanna Gambarotta, Stefano Geuna, Stefania Raimondo
Summary: This study found that neurodynamic treatment (NDT) promotes nerve regeneration, accelerates sensory and motor recovery, and modulates mechanical pain by affecting the expression of TACAN in the DRG.
Article
Biochemistry & Molecular Biology
Rui D. Alvites, Mariana V. Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Justina Prada, Isabel Pires, Giulia Ronchi, Stefania Raimondo, Ana L. Luis, Stefano Geuna, Artur Severo P. Varejao, Ana Colette Mauricio
Summary: Cell secretome is of great interest in Regenerative Medicine. This study analyzed the secretome collected from Olfactory Mucosa Mesenchymal Stem Cells and Olfactory Ensheathing Cells and applied it to promote peripheral nerve regeneration. The results showed that this therapeutic combination had beneficial effects on nerve and muscle regeneration, but further research is needed to address irregularities in the outcomes.
Review
Surgery
Stefano Artiaco, Francesco Bosco, Alessandro Lusso, Luigi Luca Cioffi, Bruno Battiston, Alessandro Masse
Summary: Closed injuries of the finger flexor pulley system are rare among the general population, but more common in rock climbers. The systematic review showed that both conservative and surgical treatments can achieve satisfactory functional outcomes for finger pulley injuries.
JOURNAL OF HAND AND MICROSURGERY
(2023)
Review
Biochemistry & Molecular Biology
Giulia Ronchi, Federica Fregnan, Luisa Muratori, Giovanna Gambarotta, Stefania Raimondo
Summary: Regeneration of damaged peripheral nerves is a major challenge in neurosurgery and regenerative medicine. Various strategies, such as tissue-engineered nerve conduits and new interventions, are being developed to improve nerve functionality and patients' life quality. However, the diversity of evaluation methods and technologies across different labs makes it difficult to compare results. This review aims to discuss the usefulness and limitations of main morphological approaches used to evaluate nerve fiber regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Marwa El Soury, Oscar Dario Garcia-Garcia, Isabella Tarulli, Jesus Chato-Astrain, Isabelle Perroteau, Stefano Geuna, Stefania Raimondo, Giovanna Gambarotta, Victor Carriel
Summary: Hollow conduits have not been as successful as autografts in regenerating critical nerve gaps. Enriching conduits with extracellular materials and cells could improve their performance. In this study, the addition of fibrin-collagen hydrogels and adipose-derived mesenchymal stem cells showed promising results in early nerve regeneration.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
David Munoz-Rodriguez, Marwane Bourqqia-Ramzi, Maria Teresa Garcia-Esteban, Antonio Murciano-Cespedosa, Alejandro Vian, Juan Lombardo-Hernandez, Pablo Garcia-Perez, Francisco Conejero, Alvaro Mateos Gonzalez, Stefano Geuna, Celia Herrera-Rincon
Summary: Inter-cellular communication involve biochemical, biophysical, and bioelectrical signals, which can occur between cells from the same tissue or animal species, as well as between cells that are evolutionarily distant. This paper presents a method for assessing the bioelectrical state of two bacterial strains and monitoring their bioelectrical profile during growth, revealing functional links between membrane potential and population growth. The study also demonstrates that bacteria respond to neural signals by changing their membrane potential, highlighting the potential for studying the relationship between bacteria and the brain in the context of the gut microbiota-brain axis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Yuki Shirosaki, Federica Fregnan, Luisa Muratori, Saki Yasutomi, Stefano Geuna, Stefania Raimondo
Summary: In this study, porous chitosan-GPTMS hybrids with different amounts of GPTMS were prepared. The structures of the degradation products with Si from the hybrids were analyzed using mass spectrometry, and their potential use in nerve repair devices was evaluated through biological assessments. The results showed that lower-molecular-weight degradation products with Si improved motor cell line differentiation and neurite outgrowth of dorsal root ganglion explants.
Review
Biochemistry & Molecular Biology
Patricia Sousa, Bruna Lopes, Ana Catarina Sousa, Alicia Moreira, Andre Coelho, Rui Alvites, Nuno Alves, Stefano Geuna, Ana Colette Mauricio
Summary: This article presents a systematic review of studies on the use of exosomes for wound healing over the past five years. The review shows that exosome-based treatments can improve wound healing by promoting angiogenesis, reepithelization, collagen deposition, and reducing scar formation. However, further research is needed due to the variability in cell sources, biomaterials, and administration routes.
Meeting Abstract
Cell & Tissue Engineering
Oscar-Dario Garcia-Garcia, David Sanchez-Porras, Marwa El-Soury, Jesus Chato-Astrain, Stefania Raimondo, Fernando Campos, Salvador Oyonarte, M. Carmen Sanchez-Quevedo, Stefano Geuna, Victor Carriel
TISSUE ENGINEERING PART A
(2022)