Article
Biochemistry & Molecular Biology
Andrea Ribeiro, Feiyue Liu, Matthias Srebrzynski, Simone Rother, Karina Adamowicz, Marta Wadowska, Stefanie Steiger, Hans-Joachim Anders, Christoph Schmaderer, Joanna Koziel, Maciej Lech
Summary: In this study, the impact of uremic toxin indoxyl sulfate on macrophages and tubular epithelial cells and its role in modulating the response to lipopolysaccharide (LPS) were investigated. Indoxyl sulfate, which accumulates in the blood of patients with chronic kidney disease (CKD), was found to induce low-grade inflammation and modulate macrophage function. Additionally, it was shown to enhance the inflammatory response associated with LPS and contribute to the senescence of tubular epithelial cells during injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Nicolas Fabresse, Islam Amine Larabi, Emuri Abe, Elodie Lamy, Claire Rigothier, Ziad A. Massy, Jean-Claude Alvarez
Summary: The objective of this study was to investigate the correlation between saliva concentration and free serum concentration of 10 uremic toxins (UTs). Saliva and blood samples from 18 healthy volunteers were collected and analyzed using LC-MS/MS. The results showed a significant correlation between saliva and serum for six UTs, while the correlation for other substances was weak or not evident. This study is the first to report the saliva concentrations of TMAO, KYN, HA, PAGN, pCG, and 3-IAA.
Article
Biochemistry & Molecular Biology
Gianvito Caggiano, Loredana Amodio, Alessandra Stasi, Nicola Antonio Colabufo, Santina Colangiulo, Francesco Pesce, Loreto Gesualdo
Summary: In recent years, indoxyl sulfate has been found to play a significant role in the progression of kidney disease and the development of cardiovascular problems. However, current methods for measuring indoxyl sulfate levels, such as LC-MS/MS, are time-consuming and require specialized equipment. In this pilot study, we developed a fast and simple alternative method using colorimetric assay to accurately measure indoxyl sulfate levels in serum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Urology & Nephrology
Lama Nazzal, Leland Soiefer, Michelle Chang, Farah Tamizuddin, Daria Schatoff, Lucas Cofer, Maria E. Aguero-Rosenfeld, Albert Matalon, Bjorn Meijers, Robert Holzman, Jerome Lowenstein
Summary: The study demonstrated that suppressing the microbiome with vancomycin led to changes in multiple gut-derived uremic solutes, but the recovery of solutes varied significantly among subjects.
KIDNEY INTERNATIONAL REPORTS
(2021)
Article
Food Science & Technology
Suguru Yamamoto, Kenichi Sasahara, Mio Domon, Keiichi Yamaguchi, Toru Ito, Shin Goto, Yuji Goto, Ichiei Narita
Summary: This study found that acidic and alkaline pH conditions changed the protein binding property of protein-bound uremic toxins (PBUTs), affecting the conformation of albumin and its binding to PBUTs.
Article
Microbiology
Ina Nemet, Masanori Funabashi, Xinmin S. Li, Mohammed Dwidar, Naseer Sangwan, Sarah M. Skye, Kymberleigh A. Romano, Tomas Cajka, Brittany D. Needham, Sarkis K. Mazmanian, Adeline M. Hajjar, Federico E. Rey, Oliver Fiehn, W. H. Wilson Tang, Michael A. Fischbach, Stanley L. Hazen
Summary: pCS and IS, gut microbiome-derived metabolites, are associated with cardiovascular disease risks and all-cause mortality. Microbial genes involved in the production of these metabolites can confer pro-thrombotic phenotype in vivo. Therapeutic targeting of gut microbial pathways may be a rational approach for CVD treatment.
Review
Biochemistry & Molecular Biology
Kuo-Chin Hung, Wei-Cheng Yao, Yi-Lien Liu, Hung-Jen Yang, Min-Tser Liao, Keong Chong, Ching-Hsiu Peng, Kuo-Cheng Lu
Summary: Patients with chronic kidney disease (CKD) often have a high accumulation of protein-bound uremic toxins (PBUTs), such as indoxyl sulfate (IS) and p-cresyl sulfate (pCS). The buildup of PBUTs inhibits bone and muscle function, worsens muscle wasting, and contributes to low bone turnover disorders. Understanding the underlying mechanisms of bone and muscle loss in CKD can aid in developing new therapies for musculoskeletal diseases.
Article
Food Science & Technology
Carolla El Chamieh, Islam Amine Larabi, Solene M. Laville, Christian Jacquelinet, Christian Combe, Denis Fouque, Maurice Laville, Luc Frimat, Roberto Pecoits-Filho, Celine Lange, Benedicte Stengel, Natalia Alencar De Pinho, Jean-Claude Alvarez, Ziad A. Massy, Sophie Liabeuf
Summary: Proton-pump inhibitors (PPIs) are commonly used in patients with chronic kidney disease (CKD), and are associated with the retention of uremic toxins (UTs). In this cross-sectional study, we found that patients using PPIs had higher levels of certain UTs compared to other patients. These findings are important for understanding factors that modulate serum UT concentration in CKD patients.
Article
Food Science & Technology
Jia-Huang Chen, Chih-Kang Chiang
Summary: Uremic toxins are harmful metabolites that accumulate in the body of patients with declining renal function, particularly in chronic kidney disease (CKD) patients. Clearing uremic toxins has become a conventional treatment in the clinic, but the clearance of uremic toxins in the early stage of acute kidney injury (AKI) is rarely emphasized, despite increasing evidence showing its correlation with injury severity or mortality.
Article
Food Science & Technology
Kullaya Takkavatakarn, Pongpratch Puapatanakul, Jeerath Phannajit, Warumphon Sukkumme, Pajaree Chariyavilaskul, Patita Sitticharoenchai, Asada Leelahavanichkul, Pisut Katavetin, Kearkiat Praditpornsilpa, Somchai Eiam-Ong, Paweena Susantitaphong
Summary: Sevelamer therapy was more effective than calcium carbonate therapy in reducing the protein-bound uremic toxin p-cresyl sulfate. Sevelamer also helped lower fibroblast growth factor 23 and low-density lipoprotein cholesterol levels, showing benefits in terms of retarding CKD progression. No changes in vascular stiffness were found in this study.
Article
Medicine, Research & Experimental
Keisuke Nakagawa, Mayuko Itoya, Nao Takemoto, Yuika Matsuura, Masashi Tawa, Yasuo Matsumura, Mamoru Ohkita
Summary: This study showed that IS increases O-2(-) production in vascular tissues, attenuating ACh- and SNP-induced vasorelaxation via potential NO inactivation. Additionally, IS-promoted O-2(-) production in the presence of vascular endothelium is likely through binding to AhR and activation of NADPH oxidase.
Article
Cardiac & Cardiovascular Systems
Kazutoshi Yamaguchi, Maimaiti Yisireyili, Sumie Goto, Xian Wu Cheng, Takayuki Nakayama, Tadashi Matsushita, Toshimitsu Niwa, Toyoaki Murohara, Kyosuke Takeshita
Summary: In patients with chronic kidney diseases, high levels of serum indoxyl sulfate (IS) are strongly associated with cardiac fibrosis and hypertrophy, which are critical risk factors for heart failure. This study found that IS can induce cardiac fibrosis and hypertrophy, impair left ventricular function, and activate the NLRP3 inflammasome pathway via the AHR/NF-kappa B pathway.
CARDIOVASCULAR TOXICOLOGY
(2022)
Article
Food Science & Technology
Jasia King, Silvia M. Mihaila, Sabbir Ahmed, Roman Truckenmuller, Stefan Giselbrecht, Rosalinde Masereeuw, Aurelie Carlier
Summary: The research highlights the inadequacy of traditional dialysis for end-stage kidney disease and emphasizes the importance of computational models in developing alternative therapies. Experimental validation of the organic anionic transporter 1 model for indoxyl sulfate transport demonstrates the significant influence of transporter density and dissociation rate on toxin removal.
Article
Urology & Nephrology
Joshua A. Walker, Sean Richards, Stephen A. Whelan, Sung Bok Yoo, Teresa L. Russell, Nkiruka Arinze, Saran Lotfollahzadeh, Marc A. Napoleon, Mostafa Belghasem, Norman Lee, Laura M. Dember, Katya Ravid, Vipul C. Chitalia
Summary: The study found that patients with chronic kidney disease are at increased risk of thrombosis after vascular injury, with indoleamine 2,3-dioxygenase-1 (IDO-1) playing a key role in this process. Inhibiting IDO-1 expression can reduce tissue factor expression in blood vessels, thereby reducing the risk of thrombosis.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Review
Urology & Nephrology
Kullaya Takkavatakarn, Thunyatorn Wuttiputinun, Jeerath Phannajit, Kearkiat Praditpornsilpa, Somchai Eiam-Ong, Paweena Susantitaphong
Summary: Prebiotics, synbiotics, and AST-120 were found to effectively reduce both serum indoxyl sulfate and p-cresyl sulfate levels in CKD patients compared to placebo. Preservation of residual renal function was associated with lower levels of these protein-bound uremic toxins. The effectiveness of biotic supplements was only observed in dialysis patients. Further studies are needed to evaluate the efficacy in non-dialysis CKD patients due to limited data.
JOURNAL OF NEPHROLOGY
(2021)
Correction
Urology & Nephrology
Keiichi Matsuzaki, Ryousuke Aoki, Yoshihito Nihei, Hitoshi Suzuki, Masao Kihara, Takashi Yokoo, Naoki Kashihara, Ichiei Narita, Yusuke Suzuki
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2022)
Article
Urology & Nephrology
Reiko Okubo, Shu-Ling Hoshi, Tomokazu Kimura, Masahide Kondo, Koichi Asahi, Chiho Iseki, Shouichi Fujimoto, Ichiei Narita, Hiroyuki Nishiyama, Kunihiro Yamagata, Kunitoshi Iseki
Summary: This study evaluated the cost-effectiveness of mass screening for dipstick hematuria tests in addition to the Specific Health Checkup (SHC) program in Japan. The results showed that adding the dipstick hematuria test to the SHC program is cost-saving and efficient in utilizing healthcare resources. The findings have implications for mass screening programs not only in Japan but worldwide.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2022)
Article
Urology & Nephrology
Keiichi Matsuzaki, Ryousuke Aoki, Yoshihito Nihei, Hitoshi Suzuki, Masao Kihara, Takashi Yokoo, Naoki Kashihara, Ichiei Narita, Yusuke Suzuki
Summary: A survey conducted by the Japanese Society of Nephrology revealed that most patients with gross hematuria after receiving the COVID-19 vaccine were already diagnosed with IgAN. Some of these patients developed proteinuria and hematuria after vaccination, but there were no cases of severe renal dysfunction observed.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2022)
Letter
Endocrinology & Metabolism
Minako Wakasugi, Akio Yokoseki, Masakazu Wada, Takeshi Momotsu, Kenji Sato, Hiroyuki Kawashima, Kazutoshi Nakamura, Osamu Onodera, Ichiei Narita
JOURNAL OF BONE AND MINERAL METABOLISM
(2022)
Article
Endocrinology & Metabolism
Minako Wakasugi, Akio Yokoseki, Masakazu Wada, Takeshi Momotsu, Kenji Sato, Hiroyuki Kawashima, Kazutoshi Nakamura, Osamu Onodera, Ichiei Narita
Summary: Polypharmacy is associated with an increased risk of fragility fracture, especially in patients with hyperpolypharmacy. The impact of hyperpolypharmacy on incident fragility fractures is particularly strong in dialysis-dependent CKD patients.
JOURNAL OF BONE AND MINERAL METABOLISM
(2022)
Article
Pharmacology & Pharmacy
Mitsuboshi Satoru, Ryohei Kaseda, Ichiei Narita
Summary: This study evaluated the association between the use of SGLT-2 inhibitors and the occurrence of drug-induced kidney injury. The findings suggest that the use of SGLT-2 inhibitors may reduce the risk of drug-induced AKI caused by certain drugs.
JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Transplantation
Minako Wakasugi, Junichiro James Kazama, Kosaku Nitta, Ichiei Narita
Summary: Smoking is significantly associated with the risk of hospitalization for any fracture in hemodialysis patients.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Hematology
Kou Kitabayashi, Suguru Yamamoto, Ichiei Narita
Summary: This study found that intake of enteral formulation by tube feeding is an independent factor associated with serum magnesium levels in patients undergoing hemodialysis, which may help in managing magnesium levels in these patients.
THERAPEUTIC APHERESIS AND DIALYSIS
(2022)
Article
Hematology
Nobuyuki Shirai, Suguru Yamamoto, Yutaka Osawa, Atsuhiro Tsubaki, Shinichiro Morishita, Ichiei Narita
Summary: The balance functions of HD patients are impaired compared with ND-CKD patients.
THERAPEUTIC APHERESIS AND DIALYSIS
(2023)
Article
Genetics & Heredity
Hirofumi Watanabe, Shin Goto, Michihiro Hosojima, Hideyuki Kabasawa, Naofumi Imai, Yumi Ito, Ichiei Narita
Summary: We report a case of Alport syndrome with a family history of impaired glucose tolerance. Whole exome sequencing identified a novel heterozygous variant of the COL4A5 gene and a rare variant of the GCK gene as the causes of Alport syndrome and monogenic diabetes, respectively. Two independent pathogenic variants affected the clinical phenotypes. Clinical next-generation sequencing is helpful for identifying the causes of patients' manifestations.
HUMAN GENOME VARIATION
(2023)
Article
Urology & Nephrology
Yuya Suzuki, Tadashi Otsuka, Yuki Yoshioka, Tomomichi Iida, Shingo Maruyama, Hirofumi Watanabe, Ryohei Kaseda, Suguru Yamamoto, Yoshikatsu Kaneko, Shin Goto, Ryuji Aoyagi, Ichiei Narita
Summary: This study found that the post-dialysis plasma level of hANP can reflect the fluid volume in hemodialysis patients. In the short-term observational study, patients with an hANP level >= 100 pg/mL were more likely to develop hypoxemia due to congestion. In the follow-up study, patients with an hANP level >= 100 pg/mL had higher rates of hospitalization due to AHF, CVD, and all-cause death. Cardiac dysfunctions were significantly associated with the high hANP level.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2023)
Article
Nutrition & Dietetics
Minako Wakasugi, Akio Yokoseki, Masakazu Wada, Takeshi Momotsu, Kenji Sato, Hiroyuki Kawashima, Kazutoshi Nakamura, Osamu Onodera, Ichiei Narita
Summary: This Japanese hospital-based study found that consuming fewer vegetables and fruits is associated with a higher risk of death, regardless of the presence of chronic kidney disease.
JOURNAL OF RENAL NUTRITION
(2023)
Article
Urology & Nephrology
Nobuyuki Shirai, Suguru Yamamoto, Yutaka Osawa, Atsuhiro Tsubaki, Shinichiro Morishita, Tsubasa Sugahara, Ichiei Narita
Summary: The frequency of falls in hemodialysis patients was associated with muscle strength and physical function, but not muscle mass.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2023)
Article
Endocrinology & Metabolism
Hirotaka Komaba, Junhui Zhao, Angelo Karaboyas, Suguru Yamamoto, Indranil Dasgupta, Mohamed Hassan, Li Zuo, Anders Christensson, Christian Combe, Bruce M. Robinson, Masafumi Fukagawa
Summary: This study analyzed the association between the prescription of active vitamin D and fracture incidence in hemodialysis patients. The results suggested that active vitamin D does not have a PTH-independent benefit in fracture prevention, supporting the current guideline of using active vitamin D only in patients with elevated or rising PTH.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Urology & Nephrology
Yusuke Nakagawa, Ryohei Kaseda, Yuya Suzuki, Hirofumi Watanabe, Tadashi Otsuka, Suguru Yamamoto, Yoshikatsu Kaneko, Shin Goto, Yasuhiko Terada, Tomoyuki Haishi, Susumu Sasaki, Ichiei Narita
Summary: This study investigates the changes in sodium concentrations in mice under normal conditions and in a diabetic mouse model using sodium magnetic resonance imaging. The results show that sodium concentrations in the kidney are decreased in both normal and diabetic conditions. This imaging technique can be useful for evaluating functional changes in diabetic kidney disease.