Article
Plant Sciences
Chakradhar Mattupalli, Fernanda Proano Cuenca, Jason B. Shiller, Tara Watkins, Karen Hansen, Carla D. Garzon, Stephen M. Marek, Carolyn A. Young
Summary: This study developed SSR markers and identified mating type genes in P. omnivora. The research showed that the fungus has high genetic diversity in the United States, with different mating types coexisting in the same field and spore mats.
Article
Agronomy
Miriam Diaz-Diaz, Alexander Bernal-Cabrera, Antonio Trapero, Alfredo Jimenez Gonzalez, Ricardo Medina-Marrero, Rene Dionsio Cupull-Santana, Edisleidy Aguila-Jimerez, Carlo Augusti-Brisach
Summary: Soil-borne pathogens associated with root rot complex diseases have devastating effects on global common bean (Phaseolus vulgaris L.) yield. In this study, the potential of Streptomyces sp. strains CBQ-EA2 and CBQ-B-8 as biological control agents (BCAs) against root rot complex disease of P. vulgaris was evaluated under natural field conditions in Cuba. Seed treatments with these strains showed significantly higher efficacy in disease reduction than the chemical control and had similar effects as the BCA T. harzianum A-34. The combination of the two Streptomyces strains exhibited the best results in disease reduction and improved germination, yield, and quality of legumes. The findings suggest that microbial consortia should be further researched for the development of biological products for crop protection.
Article
Biochemistry & Molecular Biology
Lixia Tian, Jiarong Ou, Xiao Sun, Yujing Miao, Jin Pei, Lei Zhao, Linfang Huang
Summary: This study investigated the changes in soil microbial community and physiochemical properties between healthy and rot root American ginseng, revealing significant differences in soil properties and fungal diversity. The study identified key fungal genera associated with healthy and rot root, and highlighted the potential pathogenic role of certain fungi in ginseng rot root. Additionally, the study found that soil cation exchange capacity was a crucial factor affecting the composition of microbial communities in rot root soil.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Multidisciplinary Sciences
Ranjita Sinha, Vadivelmurugan Irulappan, Basavanagouda S. Patil, Puli Chandra Obul Reddy, Venkategowda Ramegowda, Basavaiah Mohan-Raju, Krishnappa Rangappa, Harvinder Kumar Singh, Sharad Bhartiya, Muthappa Senthil-Kumar
Summary: The combination of total rainfall from November to January and average maximum temperature of October and November is crucial in determining dry root rot (DRR) occurrence in chickpea fields. Soil factors such as phosphorus, potassium, organic carbon, and clay content are positively correlated with DRR incidence, while soil silt content is negatively correlated.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Gyeongjun Cho, Da-Ran Kim, Youn-Sig Kwak
Summary: Ginseng is a popular medicinal herb with various therapeutic effects, but slow growth due to soilborne pathogens has been a challenge for its cultivation. This study investigated root rot disease and the associated microbiota in a ginseng monoculture system. The results showed that an early collapse of the microbiota community can inhibit root rot disease, and nitrogen fixation is essential for the initial microbiota community structure. The findings suggest that specific management practices to maintain a healthy microbiome can be implemented to prevent and mitigate the disease.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Kaori Okada, Wenjing Xu, Kohei Mishina, Youko Oono, Tsuneo Kato, Kiyoshi Namai, Takao Komatsuda
Summary: This study revealed the mechanism of resistance against the Japanese soil-borne wheat mosaic virus. Using quantitative PCR assay, it was found that the resistance acts directly against the virus rather than its fungal vector. By comparing different barley cultivars and wild barley, different genes were identified for the suppression of the virus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Terufumi Tada, Chihiro Tanaka, Tomoyuki Katsube-Tanaka, Tatsuhiko Shiraiwa
Summary: The study found that the development of Phytophthora root and stem rot (PRSR) in soybeans is influenced by the degree of wounding and relative humidity. High humidity stimulates disease incidence. The mortality and infection rates of wounded seedlings vary depending on the depth of the wound and humidity levels.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Microbiology
Liangliang Dai, Sunil K. K. Singh, Hao Gong, Yuanyuan Tang, Zhigang Peng, Jun Zhang, Dousheng Wu, Huiming Zhang, Danxia He
Summary: This study analyzed the rhizosphere microbiome and predicted microbial protein function in tiger lily planted with the continuous cropping method in different types of soil. The analysis revealed that bacteria from Pseudomonas and Streptomyces genera, with pathogenic functions, dominated the tiger lily rhizosphere, while bacteria from the Flavobacterium genus, with predicted phosphate transport function, specifically accumulated in maize rhizosphere. These findings suggest that continuous cropping may induce root rot disease in tiger lily through Pseudomonas and Streptomyces bacteria, and Flavobacterium bacteria may protect maize from pathogenic bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Shaohua Ge, Jian Gao, Dong Chang, Tianyi He, Heqing Cai, Maoxian Wang, Caibin Li, Zhenbao Luo, E. Yang, Jun Meng, Mingxuan Gao
Summary: This study analyzed the tobacco root microbiota in a continuous-cropping tobacco field with and without biochar treatment. The results showed that biochar application reduced the abundance of soil fungal pathogens and decreased the incidence of root rot. The study also demonstrated that the regulation of soil polyphenol oxidase (PPO) activity by biochar played a key role in suppressing pathogens. The findings suggest that biochar application can be an effective strategy for improving continuous-cropping soil.
Article
Multidisciplinary Sciences
Xuejiang Zhang, Heyun Wang, Yawei Que, Dazhao Yu, Hua Wang
Summary: Wheat root rot disease caused by soil-borne fungal pathogens leads to significant yield losses worldwide. The relationship between rhizosphere soil fungal diversity and wheat roots is crucial in understanding the occurrence of the disease. Differences in fungal diversity were observed between healthy and diseased wheat roots at different growth stages, with soil pH, nitrogen levels, and soil density playing important roles in the occurrence of wheat root rot disease.
Article
Biochemical Research Methods
Christian Froeschel
Summary: This study introduces a novel root infection system using Verticillium longisporum on Arabidopsis thaliana, while also optimizing two existing systems. The results validate the importance of indole-glucosinolates as secondary metabolites that limit the propagation of Verticillium in its host plant. Detailed assistance on studying host defence strategies and responses against V. longisporum is provided, with the potential for further research on root-microbe interactions with other soil-borne microorganisms or model plants.
Article
Agronomy
Yan Zhang, Jiaping Liang, Zhenya Tang, Qiliang Yang
Summary: Panax notoginseng is an important medicinal crop in China, but root rot during the rainy season has hindered its development. This study investigated the effects of rainfall and fertilizer on root exudates, soil bacterial structure, and root rot in P. notoginseng. The results showed that a rain shelter had a greater impact than fertilizer, and the combination of both further reduced root rot incidence. The DWF treatment decreased soil moisture, phenolic acid concentration, and root rot incidence, while promoting the growth of beneficial bacteria, leading to the lowest incidence of root rot.
Article
Plant Sciences
Alessandro Infantino, Virgilio Balmas, Nicola Schianchi, Stefano Mocali, Carolina Chiellini, Diana Martignoni, Maria Pia Aleandri, Laura Tomassoli, Anita Haegi, Alessandro Grottoli, Gabriele Chilosi
Summary: Monosporascus root rot and vine decline of melon (MRRVD) is a destructive disease complex mainly occurring in semiarid cultivation areas. A two-year survey in Sardinia found that different soil-borne fungal pathogens were associated with MRRVD in different locations and cropping seasons, with Olpidium bornovanus and O. virulentus possibly playing a key role in disease development. Soil fungal diversity may be related to the development of the disease.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Microbiology
Matej Panek, Marie Manasova, Jana Wenzlova, Miloslav Zouhar, Jana Mazakova
Summary: This study investigated the spectrum of pathogens causing crown rot on strawberry plants in the Czech Republic. A total of 24 species of Phytophthora, Phytopythium, Pythium, and Globisporangium were identified from 41 farms. Phytophthora cactorum was the most common species, found in 61% of farms. The differences in the species spectra between farms were attributed to variations in cardinal growth temperatures and farm management practices.
Article
Plant Sciences
Panpan Wang, Lifang Yang, Jialing Sun, Ye Yang, Yuan Qu, Chengxiao Wang, Diqiu Liu, Luqi Huang, Xiuming Cui, Yuan Liu
Summary: Our study revealed that the microbial community in the rhizosphere soil of Panax notoginseng is more diverse than the root endophyte community. Certain microbes such as Rhodopseudomonas, Actinoplanes, Burkholderia, and Variovorax paradoxus help P. notoginseng resist root rot disease. Additionally, upregulation of amino acid transport and metabolism in the soil can improve the resistance of P. notoginseng.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Pedro E. Gundel, Prudence Sun, Nikki D. Charlton, Carolyn A. Young, Tom E. X. Miller, Jennifer A. Rudgers
Article
Biochemistry & Molecular Biology
David J. Winter, Nikki D. Charlton, Nick Krom, Jason Shiller, Clive H. Bock, Murray P. Cox, Carolyn A. Young
MOLECULAR PLANT-MICROBE INTERACTIONS
(2020)
Article
Ecology
K. Colton Flynn, Yuting Zhou, Prasanna H. Gowda, Corey A. Moffet, Pradeep Wagle, Vijaya G. Kakani
RANGELAND ECOLOGY & MANAGEMENT
(2020)
Article
Mycology
Nikki D. Charlton, Mihwa Yi, Clive H. Bock, Minling Zhang, Carolyn A. Young
Article
Plant Sciences
Clive H. Bock, Carolyn A. Young, Minling Zhang, Chunxian Chen, Phillip M. Brannen, Jim Adaskaveg, Nikki D. Charlton
Summary: The study characterized the mating-type loci in Venturia carpophila, assessed its population genetic diversity and structure, and identified three genetic clusters among the isolates from various locations in the eastern United States. These findings provide a basis for further understanding the reproductive methods and genetics of the peach scab pathogen.
Review
Plant Sciences
Pankaj Trivedi, Chakradhar Mattupalli, Kellye Eversole, Jan E. Leach
Summary: Harnessing plant-associated microbiomes is a crucial strategy for sustainable agricultural production, but challenges remain in commercialization of microbial-based products. Translating microbiome knowledge into real-world applications will require interdisciplinary research and training the next generation of scientists.
Article
Plant Sciences
Chakradhar Mattupalli, Jason B. Shiller, Prasanna Kankanala, Nick Krom, Stephen M. Marek, Kirankumar S. Mysore, Carolyn A. Young
Summary: Phymatotrichopsis omnivora, a destructive plant pathogen causing root rot disease in various important dicotyledonous species, has been studied by generating draft genome sequences of four isolates obtained from cotton and alfalfa in Texas and Oklahoma, providing new insights into the biology of the fungus.
Article
Agriculture, Dairy & Animal Science
Matthew R. Beck, Stacey A. Gunter, Corey A. Moffet, R. Ryan Reuter
Summary: The experiment aimed to determine if dosing titanium dioxide through an automated head chamber system is an acceptable method to measure fecal output. Results showed that the fecal output estimates by hand feeding and GreenFeed methods were similar, but there was a difference in variability between the dosing methods due to varying dosing times-of-day for the GreenFeed heifers. Future experimental designs should consider the increased variability in fecal output estimates when using a GreenFeed system configured with multiple feed hoppers.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Plant Sciences
Chakradhar Mattupalli, Fernanda Proano Cuenca, Jason B. Shiller, Tara Watkins, Karen Hansen, Carla D. Garzon, Stephen M. Marek, Carolyn A. Young
Summary: This study developed SSR markers and identified mating type genes in P. omnivora. The research showed that the fungus has high genetic diversity in the United States, with different mating types coexisting in the same field and spore mats.
Article
Genetics & Heredity
Daniel Berry, Kate Lee, David Winter, Wade Mace, Yvonne Becker, Padmaja Nagabhyru, Artemis D. Treindl, Esteban Valverde Bogantes, Carolyn A. Young, Adrian Leuchtmann, Linda J. Johnson, Richard D. Johnson, Murray P. Cox, Christopher L. Schardl, Barry Scott
Summary: Fungi from the genus Epichloe form endobiotic infections in cool season grasses and produce host-protective natural products. During the sexual cycle, they exhibit virulent growth, enveloping and sterilizing the host inflorescence. RNAseq analysis identified a core set of differentially expressed genes associated with host suppression, digestion, adaptation, and stroma formation.
G3-GENES GENOMES GENETICS
(2022)
Article
Plant Sciences
Clive H. Bock, Enrique Frusso, Roberto Zoppolo, Edson R. Ortiz, Jason Shiller, Nikki D. Charlton, Carolyn A. Young, Jennifer J. Randall
Summary: This study used microsatellites to describe the genetic diversity and population structure of Venturia effusa, the plant-pathogenic fungus causing scab disease in pecan trees in South America. The results showed high genetic diversity in the populations, with evidence of population differentiation but no significant correlation between genetic distance and geographic distance. Both mating types were present in all orchard populations, and their frequencies followed the expected ratio. The population genetics of South American V. effusa populations shared similarities with those previously described in the United States.
Article
Plant Sciences
Mladen Cucak, Dalphy O. C. Harteveld, Lisa Wasko DeVetter, Tobin L. Peever, Rafael de Andrade Moral, Chakradhar Mattupalli
Summary: Mummy berry, caused by Monilinia vaccinii-corymbosi, is the most important disease of blueberry in North America. Current management strategies rely on prophylactic fungicide sprays, but predicting the timing of ascospore release and primary infection risk can improve these strategies.
Article
Microbiology
Mathieu Quenu, Artemis D. Treindl, Kate Lee, Daigo Takemoto, Torsten Thuenen, Samad Ashrafi, David Winter, Austen R. D. Ganley, Adrian Leuchtmann, Carolyn A. Young, Murray P. Cox
Summary: Genome rearrangements are common in filamentous fungi, but little is known about their evolution. In this study, we compared 15 complete genomes of the filamentous fungus genus Epichloe and found that the distinction between gene-rich and repeat-rich regions observed in isolated species is widespread across the Epichloe genus. Despite having the same number of nuclear chromosomes, the genomes of Epichloe species show low synteny and substantial rearrangement of gene content. These rearrangements are lineage-dependent and occur over short evolutionary distances with long periods of structural stability.
Article
Ecology
Stella M. Copeland, Kirk W. Davies, Stuart P. Hardegree, Corey A. Moffet, Jonathan D. Bates
Summary: This study investigated the effects of seasonal weather and plant associations on herbaceous production dynamics in sagebrush steppe. The results showed that spring precipitation was the most consistent predictor of production.
RANGELAND ECOLOGY & MANAGEMENT
(2022)