Article
Plant Sciences
Yunting Lei, Fuhan Yang, Zhaoyao Yu, Tengzhi Xu, Wene Zhang, Fenghua Tian, Xiangru Chen
Summary: In this study, Canna yellow streak virus (CaYSV) was detected in China, and an efficient method for its detection was developed. The method demonstrated high specificity and sensitivity, and field samples showed high infection rates of CaYSV on canna plants.
Article
Plant Sciences
Weijie Jin, Yubao Zhang, Xuesi Su, Ruoyu Wang, Zhongkui Xie, Yajun Wang, Yang Qiu
Summary: In this study, a rapid colloidal gold immunochromatography (GICA) detection system and a real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) system were developed and optimized for detecting LycMoV in Angelica sinensis. These sensitive detection methods will help improve virus disease management in A. sinensis crops.
Article
Biochemical Research Methods
Latief A. Wani, Priyanka Jawa, Jawaid A. Khan
Summary: Apple mosaic virus (ApMV) and Prunus necrotic ringspot virus (PNRSV), belonging to genus Ilarvirus, cause significant losses to rose and other plants of the family Rosaceae. In this study, two primer sets were designed for the development of a colorimetric Reverse Transcriptase - Loop Mediated Isothermal Amplification (RT-LAMP) assay, which showed virus-specificity and high sensitivity for early detection of ApMV and PNRSV.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Biotechnology & Applied Microbiology
Midhuna Madhu Kovileri, Smita Nair, Vimi Loius
Summary: The Banana bract mosaic virus (BBrMV) is a major virus affecting bananas and plantains. A one-step Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) assay was optimized for easy and quick detection of BBrMV in banana. The assay showed high specificity and successfully detected the virus from symptomatic samples.
Article
Agriculture, Multidisciplinary
Yubao Zhang, Zhongkui Xie, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yang Qiu, Yuhui He
Summary: LSV and CMV are prevalent viruses that infect lily plants, particularly impacting the yield of edible Lanzhou lilies in northwestern China. The IC-RT-LAMP assay developed in this study offers a sensitive and convenient method for detecting these viruses, with the potential to enhance diagnosis of field-collected samples where laboratory resources are limited.
ANNALS OF APPLIED BIOLOGY
(2022)
Article
Chemistry, Analytical
Wansadaj Jaroenram, Itthi Chatnuntawech, Jantana Kampeera, Sukanya Pengpanich, Pornsawan Leaungwutiwong, Benyatip Tondee, Sarawut Sirithammajak, Rapheephat Suvannakad, Pakapreud Khumwan, Sirintip Dangtip, Narong Arunrut, Sirasate Bantuchai, Wang Nguitragool, Suchawit Wongwaroran, Paisan Khanchaitit, Jetsumon Sattabongkot, Surat Teerapittayanon, Wansika Kiatpathomchai
Summary: Colorimetric loop-mediated DNA isothermal amplification-based assays using pH-dependent indicators have been developed for COVID-19 diagnosis. The dual colorimetric RT-LAMP assay with artificial intelligence (AI) operated tool enables precise and rapid result analysis in large scale testing. The method offers a simpler, faster, and cheaper approach for COVID-19 testing and other emerging pathogens.
Article
Virology
Kan Kimura, Akio Miyazaki, Takumi Suzuki, Toya Yamamoto, Yugo Kitazawa, Kensaku Maejima, Shigetou Namba, Yasuyuki Yamaji
Summary: Tomato mottle mosaic virus (ToMMV) is a seed-transmissible tobamovirus that infects tomato and pepper. A detection method based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed in this study. This method has high sensitivity, specificity, and rapidity, and can detect ToMMV within 30 minutes without the need for RNA purification. It is expected to be valuable for point-of-care testing in field surveys and large-scale testing.
Article
Chemistry, Analytical
Honghong Wang, Shuhui Wang, Hui Wang, Yuanwen Liang, Zhengping Li
Summary: Fusion genes, as causative oncogenes and drug targets, play critical roles in tumor development and treatment. A specific and sensitive method for quantifying fusion transcripts was proposed, which uses stem-loop primers to track fusion junctions and initiates reverse transcript loop-mediated isothermal amplification (LAMP). The method achieved high sensitivity and a wide linear dynamic range, simplifying the detection process and effectively eliminating false-positive results.
Article
Microbiology
Cyril Chik-Yan Yip, Siddharth Sridhar, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Kit-Hang Leung, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To
Summary: This study evaluated a fast, affordable, and easy-to-perform RT-LAMP-CRISPR diagnostic method for detecting COVID-19. The method uses primers designed for highly expressed genes and demonstrates high sensitivity and specificity, making it suitable for resource-limited settings.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agronomy
Ali Celik
Summary: A one-step colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of prune dwarf virus (PDV). The assay showed high sensitivity and specificity, making it suitable for laboratory and field use.
Article
Chemistry, Analytical
Junmin Li, Xuejiao Hu, Xiaoming Wang, Jianing Yang, Lei Zhang, Qianyun Deng, Xiqin Zhang, Zixia Wang, Tieying Hou, Shan Li
Summary: A novel one-tube rapid diagnostic approach based on formamide and colorimetric RT-LAMP was established for the detection of SARS-CoV-2 without the extraction of specific viral RNA. The technique showed high specificity and could visually detect the virus within 45 minutes.
ANALYTICA CHIMICA ACTA
(2021)
Article
Medicine, General & Internal
Juan Garcia-Bernalt Diego, Pedro Fernandez-Soto, Marta Dominguez-Gil, Moncef Belhassen-Garcia, Juan Luis Munoz Bellido, Antonio Muro
Summary: The global SARS-CoV-2 pandemic has brought about a need for widespread testing for COVID-19, with RT-qPCR as the gold standard method, but facing challenges. A novel RT-LAMP method has been developed, showing promise for cost-effective, rapid, and stable detection of SARS-CoV-2.
Article
Medicine, Research & Experimental
Yangyang Sun, Lei Yu, Chengxi Liu, Shanting Ye, Wei Chen, Dechang Li, Weiren Huang
Summary: The OR-DETECTR platform for detecting COVID-19 is rapid, accurate, and can be completed in one tube, with a low detection limit and 100% consistency with rRT-PCR results. Additionally, a lateral flow assay based on OR-DETECTR has been developed for COVID-19 detection.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Chao Ji, Shuxia Xue, Min Yu, Jinyu Liu, Qin Zhang, Feng Zuo, Qiuyue Zheng, Liangjuan Zhao, Hongwei Zhang, Jijuan Cao, Ke Wang, Wei Liu, Wenjie Zheng
Summary: The study introduced a rapid and simple method for detecting the SARS-CoV-2 virus, which can be easily detected by naked eye through color change, demonstrating high specificity and accuracy.
Review
Medicine, General & Internal
Kanny Diallo, Vitalis F. Feteh, Lilian Ibe, Martin Antonio, Dominique A. Caugant, Mignon du Plessis, Ala-Eddine Deghmane, Ian M. Feavers, Katya Fernandez, LeAnne M. Fox, Charlene M. C. Rodrigues, Olivier Ronveaux, Muhamed-Kheir Taha, Xin Wang, Angela B. Brueggemann, Martin C. J. Maiden, Odile B. Harrison
Summary: Bacterial meningitis is a significant global cause of morbidity and mortality, and rapid identification of the causative agent is crucial for clinical management and disease prevention. Molecular diagnostic assays have been developed as an alternative to microbiological culture, allowing for faster and more accurate detection of the pathogens causing bacterial meningitis.
Article
Infectious Diseases
Idrissa Dieng, Moussa Moise Diagne, Marie Henriette Dior Ndione, Boris Gildas Hedible, Mamadou Diop, Edgard Valery Adjoguoua, Yahaya Sylla, Herve Kadjo, Cheikh Loucoubar, Amadou Alpha Sall, Oumar Faye, Ousmane Faye
Summary: Dengue fever is the most common arboviral infection worldwide, but its epidemiology in Africa is still not fully understood. This study reported a case of dengue virus serotype 2 imported from Cote d'Ivoire to Senegal, suggesting a possible spread of strains from the Burkina Faso outbreak to other West African countries. The phylogenetic analysis based on full-length genome sequence revealed a clustering with strains from the Burkina Faso outbreak in 2016 and the ongoing dengue fever outbreak in Cote d'Ivoire.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Martin Faye, Oumar Faye, Nicholas Di Paola, Marie Henriette Dior Ndione, Moussa Moise Diagne, Cheikh Tidiane Diagne, Ndeye Sakha Bob, Gamou Fall, Jean-Michel Heraud, Amadou Alpha Sall, Ousmane Faye
Summary: This study investigated the genetic diversity of rabies virus in Senegal and identified a new honey-badger-related strain. The study also found amino acid polymorphisms in Senegalese rabies virus sequences and suggested the importance of surveillance in wildlife to prevent re-introduction of rabies.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Giulia Mencattelli, Marie Henriette Dior Ndione, Roberto Rosa, Giovanni Marini, Cheikh Tidiane Diagne, Moussa Moise Diagne, Gamou Fall, Ousmane Faye, Mawlouth Diallo, Oumar Faye, Giovanni Savini, Annapaola Rizzoli
Summary: The West Nile virus poses continuous challenges to public health worldwide, with new lineages and clades emerging. Current knowledge on the virus in Africa is limited, highlighting the need for further research and coordinated surveillance efforts.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Fisheries
Cuneyt Tamer, Jessica Benkaroun, Hanne Nur Kurucay, Harun Albayrak, Manfred Weidmann
Summary: Fish viral diseases cause significant economic losses in aquaculture. Viral haemorrhagic septicemia virus (VHSV) is one of the most important viruses affecting fish species. Early detection of diseases is critical for disease prevention and control. This study developed a sensitive RPA assay for detecting VHSV, which showed similar sensitivity to real-time RT-PCR.
JOURNAL OF FISH DISEASES
(2022)
Article
Infectious Diseases
Daniel Mukadi-Bamuleka, Junior Bulabula-Penge, Anja De Weggheleire, Bart K. M. Jacobs, Francois Edidi-Atani, Fabrice Mambu-Mbika, Placide Mbala-Kingebeni, Sheila Makiala-Mandanda, Martin Faye, Cheick T. Diagne, Moussa M. Diagne, Oumar Faye, Masahiro Kajihara, Ousmane Faye, Ayato Takada, Amadou A. Sall, Jean-Jacques Muyembe-Tamfum, Johan van Griensven, Kevin K. Arien, Steve Ahuka-Mundeke
Summary: The study assessed three antigen detection rapid diagnostic tests (RDTs) performance during the Ebola outbreak in the Democratic Republic of the Congo. QuickNavi-Ebola RDT showed high sensitivity and specificity, indicating its effectiveness in diagnosing Ebola virus disease.
LANCET INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Wisal A. Elmagzoub, Sanaa M. Idris, Maha Isameldin, Nassir Arabi, Abdelmonem Abdo, Mustafa Ibrahim, Md Anik Ashfaq Khan, Franziska Tanneberger, Sahar M. Bakhiet, Julius B. Okuni, Lonzy Ojok, Ahmed A. Gameel, Ahmed Abd El Wahed, Michael Bekaert, Mohamed E. Mukhtar, Ahmad Amanzada, Kamal H. Eltom, ElSagad Eltayeb
Summary: This study represents the first report of Mycobacterium avium subsp. paratuberculosis (MAP) in humans in Sudan, revealing a considerable proportion of the population may be infected or carriers of the bacteria. Increased awareness at the community level is urgently needed to reduce the risk of MAP transmission at the human-animal interface.
Article
Fisheries
John B. Taggart, Michael J. Leaver, Michael Bekaert
Summary: A non-synonymous SNP leads to a diallelic allozyme polymorphism in the mitochondrial NADP-dependent mMEP-2* locus in Atlantic salmon. The genotyping results from allozyme and SNP assays are concordant in most individuals.
JOURNAL OF FISH BIOLOGY
(2022)
Article
Biochemical Research Methods
Idrissa Dieng, Mignane Ndiaye, Moussa Dia, Moufid Mhamadi, Cheikh Talibouya Toure, Aboubacry Gaye, Cheikh Tidiane Diagne, Ahmed El Wahed, Manfred Weidmann, Ousmane Faye, Amadou Alpha Sall, Oumar Faye
Summary: A qRT-PCR system based on TaqMan chemistry was developed to detect Saboya virus (SABV) with high specificity and sensitivity. The system reliably detected SABV strains without cross amplification of other flaviviruses. It can be easily incorporated into existing arbovirus detection assays to study virus prevalence in various samples.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Fisheries
Alvaro Hernandez-Montiel, Ivone Giffard-Mena, Manfred Weidmann, Michael Bekaert, Kristina Ulrich, Jessica Benkaroun
Summary: White spot syndrome virus (WSSV) infects economically important aquaculture species and causes significant losses to the industry. The virus exhibits varying levels of virulence in different shrimp species, with differences observed in isolate virulence in Penaeus vannamei. The complete genomes of isolate strains from Mexico were sequenced, revealing deletion zones and differences in repetitive motifs that may contribute to virulence variations.
DISEASES OF AQUATIC ORGANISMS
(2022)
Article
Virology
Marie Henriette Dior Ndione, El Hadji Ndiaye, Martin Faye, Moussa Moise Diagne, Diawo Diallo, Amadou Diallo, Amadou Alpha Sall, Cheikh Loucoubar, Oumar Faye, Mawlouth Diallo, Ousmane Faye, Mamadou Aliou Barry, Gamou Fall
Summary: The study aimed to understand the transmission dynamics of West Nile virus (WNV) in Senegal. By monitoring mosquito, human, and animal samples, the research found the presence of WNV in humans and mosquitoes, as well as the exchange of WNV strains between Senegal and Europe. This highlights the importance of expanding surveillance systems to include animals in order to prepare for global health threats.
Article
Virology
Jessica Benkaroun, Sven M. Bergmann, Angela Roemer-Oberdoerfer, Menekse Didem Demircan, Cuneyt Tamer, Gayatri Rajendra Kachh, Manfred Weidmann
Summary: Lymphocystis disease viruses (LCDVs) are found in various locations worldwide and infect bony fish. The genome size of LCDVs varies, with the LCDV1 genome being approximately half the size of LCDV2-4 genomes. Whole genome sequencing and analysis of LCDVs from wild fish and aquafarmed sea bream confirm this difference in genome size and suggest an adaptation to the lifestyle of demersal fish in the northeast Atlantic.
Article
Environmental Sciences
Idrissa Dieng, Cheikh Fall, Mamadou Aliou Barry, Aboubacry Gaye, Ndongo Dia, Marie Henriette Dior Ndione, Amary Fall, Mamadou Diop, Fatoumata Diene Sarr, Oumar Ndiaye, Mamadou Dieng, Boly Diop, Cheikh Tidiane Diagne, Mamadou Ndiaye, Gamou Fall, Mbacke Sylla, Ousmane Faye, Cheikh Loucoubar, Oumar Faye, Amadou Alpha Sall
Summary: This study investigates the epidemiological and molecular evolution data of a dengue outbreak that occurred in Senegal in 2018. It reveals that the outbreak was caused by the co-circulation of dengue serotypes 1 and 3, with 263 confirmed cases and 25 severe cases and 2 deaths. Phylogenetic analysis based on the E gene identifies DENV-3 as the main serotype, belonging to Genotype III. Bayesian phylogeographic analysis suggests a viral introduction around July 2017 and subsequent cryptic circulation before the identification of the first case in October 2018. The study also highlights the importance of a mobile laboratory in outbreak response.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Idrissa Dieng, Mamadou Aliou Barry, Cheikh Talla, Bocar Sow, Oumar Faye, Moussa Moise Diagne, Ousseynou Sene, Oumar Ndiaye, Boly Diop, Cheikh Tidiane Diagne, Gamou Fall, Amadou Alpha Sall, Cheikh Loucoubar, Ousmane Faye
Summary: This study investigated an outbreak of dengue fever in Senegal and identified a serotype replacement of the dengue virus. The findings highlight the importance of improved dengue virus surveillance in Senegal and continuous monitoring of circulating serotypes for a better understanding of viral epidemiology.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Virology
Arianna Ceruti, Ndongo Dia, Adeleye Solomon Bakarey, Judah Ssekitoleko, Soa Fy Andriamandimby, Padra Malwengo-Kasongo, Rasheeda H. A. Ahmed, Rea Maja Kobialka, Jean Michel Heraud, Moussa Moise Diagne, Marie Henriette Dior Dione, Idrissa Dieng, Martin Faye, Ousmane Faye, Jean Theophile Rafisandratantsoa, Jean-Pierre Ravalohery, Claudio Raharinandrasana, Tsiry H. Randriambolamanantsoa, Norosoa Razanajatovo, Iony Razanatovo, Joelinotahina H. Rabarison, Phillipe Dussart, Louis Kyei-Tuffuor, Abigail Agbanyo, Olubusuyi Moses Adewumi, Adeola Fowotade, Muideen Kolawole Raifu, Patient Okitale-Talunda, Gracia Kashitu-Mujinga, Christelle Mbelu-Kabongo, Steve Ahuka-Mundeke, Anguy Makaka-Mutondo, Enas M. Abdalla, Sanaa M. Idris, Wisal A. Elmagzoub, Rahma H. Ali, Eman O. M. Nour, Rasha S. M. Ebraheem, Huda H. H. Ahmed, Hamadelniel E. Abdalla, Musab Elnegoumi, Izdihar Mukhtar, Muatsim A. M. Adam, Nuha Y. I. Mohamed, Shahinaz A. Bedri, Hamdan Mustafa Hamdan, Magid Kisekka, Monica Mpumwiire, Sharley Melissa Aloyo, Joanita Nabwire Wandera, Andrew Agaba, Rogers Kamulegeya, Hosea Kiprotich, David Patrick Kateete, Paul Kadetz, Uwe Truyen, Kamal H. Eltom, Anavaj Sakuntabhai, Julius Boniface Okuni, Sheila Makiala-Mandanda, Vincent Lacoste, George Olusegun Ademowo, Michael Frimpong, Amadou Alpha Sall, Manfred Weidmann, Ahmed Abd El Wahed
Summary: In this study, a novel isothermal amplification method was validated for rapid detection of SARS-CoV-2 in seven sub-Saharan African countries. The RdRP-based amplification method showed the best assay accuracy. The study highlighted the challenges of implementing rapid molecular assays in field conditions, emphasizing the importance of standardized operation procedures, continuous training for staff, and enhanced quality control measures for successful deployment.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Virology
Oumar Ndiaye, Kevin Woolston, Aboubacry Gaye, Cheikh Loucoubar, Michael Cocozza, Cheikh Fall, Fatou Dia, Emily R. Adams, Marieme Samb, Diogop Camara, Bacary Djilocalisse Sadio, Cheikh T. Diagne, Manfred Weidmann, Oumar Faye, Joseph R. A. Fitchett, Amadou Alpha Sall, Cheikh Tidiane Diagne
Summary: The burden of dengue in Senegal is increasing and expanding, making rapid diagnostic tests (RDTs) an ideal tool for investigating active outbreaks. The diagnostic performance of Dengue NS1 and Dengue IgM/IgG RDTs was evaluated in the laboratory and field settings. The results showed high sensitivity and specificity for the RDTs, making them suitable for use in high prevalence or outbreak situations where confirmatory tests may be lacking.