期刊
JOURNAL OF INTEGRATIVE AGRICULTURE
卷 17, 期 6, 页码 1289-1298出版社
ELSEVIER SCI LTD
DOI: 10.1016/S2095-3119(17)61821-5
关键词
dirigent domain; GmDRR1; soybean; Phytophthora sojae; oomycete; proteomics
资金
- Academic Skeleton Support Plan of Department of Education of Heilongjiang Province, China [1254G011]
- National Natural Science Foundation of China [31271747, 31471516, 31400074, 31401465, 31501332]
- National High-Tech R&D Program of China (the 863 Program) [2013AA102602]
- Research Fund for the Doctoral Program of Higher Education of China [20122325120015]
- Academic Backbone Project of Northeast Agricultural University, China [15XG02]
- Talented Young Project of Northeast Agricultural University, China [518062]
Soil-borne pathogen Phytophthora sojae is an oomycete that causes devastating damage to soybean yield. To mine original resistant genes in soybean is an effective and environmentally-friend approach controlling the disease. In this study, soybean proteins were extracted from the first trifoliolates infected by predominant P sojae race 1 and analyzed by two-dimensional gel electrophoresis. Nineteen differently-expressed protein spots were detected, and 10 of them were further applied for Matrix-Assisted Laser Desorption/lonization Time of Flight Mass Spectrometry Assay. One protein containing a dirigent (DIR) domain was identified and belonged to the DIR-b/d family. Therefore, it was named as GmDRR1 (gycine max Disease Resistance Response 1). Then, GmDRR1 gene was pathologically confirmed to be involved in the resistant to P sojae in soybean. GmDRR1-GFP (green fluorescent protein) fusion proteins localized in the cell membrane. qRT-PCR results showed GmDRR1 gene expressed differently in P sojae resistant- and susceptible-soybean cultivars. By the promoter analysis, we found a haplotype H8 was existing in most resistant soybean varieties, while a haplotype H77 was existing in most susceptible soybean varieties. The H77 haplotype had seven SNPs (C to A, G to C, C to A, T to A, T to C, T to C, and T to A) and two single nucleotide insertions. The results supported that the expression difference of GmDRR1 genes between P sojae resistant- and susceptible-soybean cultivars might depend on the GmDRR1 promoter SNPs. The results suggested that GmDRR1 was a dirigent protein involved in soybean resistant to P sojae and paved a novel way for investigation of the molecular regulatory mechanism of the defense response to P sojae in soybean.
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