期刊
CELL REPORTS
卷 22, 期 1, 页码 242-254出版社
CELL PRESS
DOI: 10.1016/j.celrep.2017.12.007
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资金
- NIH [C06 RR020128-01, DC006167, DC015624]
- German Research Foundation [BO 4143/1-1]
- NIDCD NRSA [DC013210]
- Ralph W. and Grace M. Showalter Trust
- Indiana CTSI [UL1 TR001108]
- Indiana Center for Biomedical Innovation (technology enhancement grant)
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR001108] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS [F32DC013210, R03DC015624, R01DC006167] Funding Source: NIH RePORTER
The mammalian hair follicle arises during embryonic development from coordinated interactions between the epidermis and dermis. It is currently unclear how to recapitulate hair follicle induction in pluripotent stem cell cultures for use in basic research studies or in vitro drug testing. To date, generation of hair follicles in vitro has only been possible using primary cells isolated from embryonic skin, cultured alone or in a co-culture with stem cell-derived cells, combined with in vivo transplantation. Here, we describe the derivation of skin organoids, constituting epidermal and dermal layers, from a homogeneous population of mouse pluripotent stem cells in a 3D culture. We show that skin organoids spontaneously produce de novo hair follicles in a process that mimics normal embryonic hair folliculogenesis. This in vitro model of skin development will be useful for studying mechanisms of hair follicle induction, evaluating hair growth or inhibitory drugs, and modeling skin diseases.
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