Article
Medicine, Research & Experimental
Lianghui Zhan, Jingru Zheng, Jianbiao Meng, Danting Fu, Lisha Pang, Chunlian Ji
Summary: This study found that TLR4 deficiency can reduce inflammatory responses and apoptosis, improve intestinal damage, regulate intestinal flora, and alleviate LPS-induced intestinal barrier dysfunction.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Oncology
Jun Ying, Haiyang Zhou, Zhiguo Wang, Qing You, Junnan Chen, Hao Lu, Jian Zhang
Summary: Background: Chemotherapy resistance is a major obstacle to the efficacy of chemotherapy in colon cancer. Therefore, improving the chemotherapy sensitivity of colorectal cancer cells is crucial for enhancing the prognosis of colon cancer patients. Methods: Cell viability of colorectal cancer cell lines was assessed using the CCK-8 assay. Toll-like receptor 4 (TLR4) expression in colorectal cancer cell lines was investigated through real-time PCR and western blot. The role of TLR4 in the stemness of colorectal cancer cell lines was analyzed by infecting cells with lentivirus containing TLR4 siRNA. Results: Aspirin was found to effectively enhance chemosensitivity and inhibit the stemness of CT26 and HCT116 colorectal cancer cell lines. Aspirin inhibited clone formation, reduced the volume and number of cell spheres, and down-regulated stemness-related genes. Moreover, aspirin also down-regulated TLR4 expression in colorectal cancer cells. TLR4 positive colorectal cancer cells demonstrated higher chemotherapy resistance potential and stronger stemness compared to TLR4 negative colorectal cancer cells. Conclusion: Our study indicates that aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Martin Beaumont, Corinne Lencina, Katia Feve, Celine Barilly, Laurence Le-Normand, Sylvie Combes, Guillaume Devailly, Gaelle Boudry
Summary: The disruption of the primocolonizing gut microbiota alters epithelial innate immunity in the colon and imprints stem cells, which could have long-term consequences for gut health. Colistin, an antibiotic, was used in a newborn piglet model to deplete specific gram-negative bacteria in the neonatal gut microbiota. The depletion of these bacteria resulted in altered gene expression in the colon epithelium, decreased toll-like receptor 4 (TLR4) and lysozyme (LYZ) levels, and an increased concentration of succinate in the colon.
Article
Immunology
Ioanna Tsioti, Beatrice L. Steiner, Pascal Escher, Martin S. Zinkernagel, Peter M. Benz, Despina Kokona
Summary: Clustering of microglia around the vasculature was observed after systemic administration of lipopolysaccharides (LPS) in the retina and brain of mice. This study investigated the effect of systemic LPS in the pigmented mouse retina and the involvement of endothelial TLR4 in LPS-induced retinal microglia activation. The results showed that activation of microglia, infiltration of monocyte-derived macrophages, impaired ribbon synapse organization, and retinal dysfunction were observed after LPS exposure, and these effects were dependent on endothelial TLR4.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Oncology
Fatima Junaid, Goran Tomic, Richard Kemp, Doug J. Winton
Summary: Using human colon cancer DLD-1 cells, the study engineered a cell line with inducible Snail expression and found that partial EMT induced by Snail overexpression in DLD-1 cells leads to chemoresistance, increased tumor growth, and decreased apoptosis, highlighting the importance of bioavailable Snail in determining the phenotypic outcome.
Article
Multidisciplinary Sciences
Ruonan Zhang, Xiaofeng Li, Jie Zhang, Yanjun Li, Yuan Wang, Yuhang Song, Feifei Ren, Huiyu Yi, Xiaojuan Deng, Yangjin Zhong, Yang Cao, Michael R. Strand, Xiao-Qiang Yu, Wanying Yang
Summary: Toll/Toll-like receptors play different roles in mammals and insects, with insects primarily activating the Toll pathway through binding Spatzle cytokines rather than directly recognizing pathogen-associated molecular patterns.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Fang Li, Haoran Ke, Siqi Wang, Wei Mao, Cexiong Fu, Xi Chen, Qingqing Fu, Xiaori Qin, Yonghua Huang, Bidan Li, Shibing Li, Jingying Xing, Minhui Wang, Wenlin Deng
Summary: Leaky gut could be a trigger for the development of autism by activating the lipopolysaccharide-mediated TLR4-MyD88-NF-kappa B pathway. Disrupting and repairing the intestinal barrier improved the behavior of autistic mice and reduced inflammation.
NEUROSCIENCE BULLETIN
(2023)
Review
Cell Biology
Nachiket M. Godbole, Asif Alam Chowdhury, Neha Chataut, Shanjana Awasthi
Summary: The lung epithelium plays a crucial role in responding to infections, toxins, and allergies, as well as maintaining normal pulmonary function. The tight junctions between lung epithelial cells are essential for maintaining the integrity and fluid balance of the lung. This review summarizes the literature on disrupted tight junctions and epithelial barrier in various lung conditions, and discusses the expression and regulation of specific tight junction proteins against pathogenic stimuli. The review also explores the molecular signaling and crosstalk among signaling pathways that control the tight junction structure and function in the lung.
Article
Oncology
Susanna Poghosyan, Nicola Frenkel, Aristeidis Lentzas, Jamila Laoukili, Inne Borel Rinkes, Onno Kranenburg, Jeroen Hagendoorn
Summary: This study investigates the role of Nrp2 in colorectal cancer (CRC) progression. Nrp2 expression is associated with aggressive CRC and poor prognosis. Using murine CRC tumor-derived organoids, the researchers demonstrate that Nrp2 depletion leads to a change in phenotype, MET, and a dependence on IR signaling and autophagy. The study highlights the complex interaction between Nrp2 and alternative pro-survival mechanisms in aggressive CRC.
Article
Health Care Sciences & Services
Constantin Busuioc, Cristina Alexandra Ciocan-Cartita, Cornelia Braicu, Oana Zanoaga, Lajos Raduly, Monica Trif, Mihai-Stefan Muresan, Calin Ionescu, Cristina Stefan, Carmen Crivii, Nadim Al Hajjar, Simona Margarit, Ioana Berindan-Neagoe
Summary: The study utilized bioinformatics approaches to investigate alterations in EMT-related genes in colon adenocarcinoma patients, identifying key gene changes related to prognosis in COAD. These findings may help develop biomarkers for recurrence prediction in COAD patients and improve risk stratification for patients.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Immunology
Wei Zhang, Eun-Koung An, Juyoung Hwang, Jun-O Jin
Summary: The study found that LPS can directly stimulate pDCs via the TLR4/MD2 pathway and promote the activation of pDCs and cDCs. cDCs may contribute to the enhancement of IFN-alpha production in pDCs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lei Na, Zhuo Wang, Yu Bai, Yu Sun, Dan Dong, Wei Wang, Chenghai Zhao
Summary: This study identified that WNT7B is downregulated in high-grade bladder urothelial carcinomas, and its low expression is associated with poor prognosis. WNT7B inhibits bladder urothelial carcinoma progression by suppressing EMT, stem-like properties, and chemoresistance.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Agriculture, Dairy & Animal Science
Wan Xie, Yang Xue, Xiaokun Song, Hongzhu Zhang, Guangjun Chang, Xiangzhen Shen
Summary: This study found that LPS activates TLR4 and inhibits FOXA2 expression, leading to NF-kappa B-mediated inflammation, ER stress, autophagy, and apoptosis. Inhibiting TLR4 and increasing FOXA2 expression can alleviate LPS-induced inflammation, ER stress, autophagy, and apoptosis, maintaining cellular homeostasis.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Medicine, Research & Experimental
Mei-Tsz Su, Pei-Yin Tsai, Chia-Yih Wang, Hui-Ling Tsai, Pao-Lin Kuo
Summary: Preeclampsia is a severe gestational hypertensive disorder that poses a major threat to maternal and fetal health. Aspirin has been shown to effectively reduce the incidence of premature preeclampsia among high-risk pregnant women, possibly by suppressing miR-200 family-mediated cell functions. This study demonstrates a potential mechanism explaining aspirin's effect on preeclampsia prevention and provides a therapeutic target for disease intervention.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Engineering, Biomedical
Zehuan Liao, Joseph Jing Heng Lim, Jeannie Xue Ting Lee, Damien Chua, Marcus Ivan Gerard Vos, Yun Sheng Yip, Choon Boon Too, Huan Cao, Jun Kit Wang, Yufeng Shou, Andy Tay, Kaisa Lehti, Hong Sheng Cheng, Chor Yong Tay, Nguan Soon Tan
Summary: The study found that mechanotransduction is elevated in 3D cultures and further intensified during EMT, but not during 2D EMT. The authors' hydrogel-based 3D cancer cultures with adjustable mechanical forces revealed Angptl4 as a promising therapeutic target to inhibit EMT and impede cancer progression.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Oncology
Qing-Mei Zhao, Fei Kuang, Han Wu, Yu-Hao Zhang
MOLECULAR MEDICINE REPORTS
(2015)
Article
Oncology
De Luo, Fei Kuang, Juan Du, Mengjia Zhou, Fangyi Peng, Yu Gan, Cheng Fang, Xiaoli Yang, Bo Li, Song Su
Summary: The study reveals a close association between immune cell infiltration patterns and prognosis in pancreatic carcinoma patients, with those having low ICI scores showing better outcomes. Furthermore, low tumor mutation burden is linked to improved overall survival. A high ICI score is characterized by reduced expression of immune-related genes, suppression of metabolic pathways, and poor prognosis.
FRONTIERS IN ONCOLOGY
(2021)
Review
Oncology
De Luo, Fei Kuang, Juan Du, Mengjia Zhou, Xiangdong Liu, Xinchen Luo, Yong Tang, Bo Li, Song Su
Summary: Based on our meta-analysis, artificial intelligence showed high accuracy in the diagnosis of early upper gastrointestinal cancer (EUGIC).
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Fei Kuang, De Luo, Mengjia Zhou, Juan Du, Jie Yang
Summary: This study analyzed the role of N6-methyladenosine- (m6A-) related long noncoding RNAs (lncRNAs) in colorectal cancer (CRC) and identified two different m6A modification patterns. A prognostic risk signature composed of six m6A-related lncRNAs was constructed. The lncRNAs AC156455.1 and AC104532.2 were found to be upregulated in colorectal cancer tissues compared to normal tissues. These findings highlight the potential of these lncRNAs as biomarkers for diagnosis and prognosis in CRC.
Review
Gastroenterology & Hepatology
De Luo, Xinpei Chen, Juan Du, Bingjie Mei, Ankang Wang, Fei Kuang, Cheng Fang, Yu Gan, Fangyi Peng, Xiaoli Yang, Uta Dahmen, Bo Li, Su Song
Summary: This study evaluated the immunogenicity of COVID-19 vaccines in chronic liver disease (CLD) patients and liver transplant (LT) recipients. The results showed that COVID-19 vaccines induced strong humoral immune responses in CLD patients but poor humoral immune responses in LT recipients.
LIVER INTERNATIONAL
(2023)