期刊
SCIENTIFIC REPORTS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-27500-3
关键词
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资金
- Agriculture and Agri-Food Canada Crop Genomics Initiative
- Canada Research Chair in Plant-Microbe Systems Biology
- Centre for the Analysis of Genome Evolution and Function
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2014-06434]
- Canada Foundation for Innovation
- Ontarian Government
- Genome Canada
- Ontario Genomics [OGI-139]
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.
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