Article
Oncology
Xiuli Zhang, Zhizhou Xia, Xingyu Lv, Donghe Li, Mingzhu Liu, Ruihong Zhang, Tong Ji, Ping Liu, Ruibao Ren
Summary: The study demonstrated that DCAF8 plays a critical role in spermatogenesis and is a key component of CRL4 in the reproductive system. Knockout of the Dcaf8 gene led to male infertility in mice, with decreased sperm motility and increased morphological abnormalities.
FRONTIERS OF MEDICINE
(2021)
Article
Developmental Biology
Brendan J. Houston, Alexandra M. Lopes, Maris Laan, Liina Nagirnaja, Anne E. O'Connor, D. Jo Merriner, Joseph Nguyen, Margus Punab, Antoni Riera-Escamilla, Csilla Krausz, Kenneth Ivan Aston, Donald F. Conrad, Moira K. O'Bryan
Summary: This study found that one of the genes associated with male infertility, DCAF12L1, does not have a significant impact on fertility in mice. This disparity may be due to differences in DCAF12 family members between mice and humans, or the presence of environmental factors in humans.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Haixia Xu, Jiahua Guo, Wei Wu, Qiu Han, Yueru Huang, Yaling Wang, Cencen Li, Xiaofang Cheng, Pengpeng Zhang, Yongjie Xu
Summary: This study reveals that hnRNPK plays a crucial role in spermatogenesis and male fertility. HnRNPK is strongly expressed in the mouse testis and is mainly localized in the nucleus of germ cells. Depletion of hnRNPK in male germ cells leads to developmental abnormalities in the testis and infertility in males. The absence of hnRNPK in spermatocytes causes meiotic arrest and impairs the production of mature sperm.
Article
Medicine, Research & Experimental
Zongzhuang Wen, Haixia Zhu, Jing Wang, Bin Wu, Aizhen Zhang, Hui Zhao, Chenyang Song, Shuangyuan Liu, Yin Cheng, Hongxiang Wang, Jianyuan Li, Daqing Sun, Xiaolong Fu, Jiangang Gao, Min Liu
Summary: This study generated a germ cell-specific deletion mutant of the Hspa5 gene (Hspa5-cKO) using CRISPR/Cas9 technology and the Cre/Loxp system. Hspa5 knockout resulted in severe germ cell loss and vacuolar degeneration of seminiferous tubules, leading to complete arrest of spermatogenesis, testicular atrophy, and male infertility in adult mice. Single-cell RNA sequencing analysis further confirmed that HSPA5 is crucial for germ cell development.
Article
Developmental Biology
Na Liu, Fatimunnisa Qadri, Hauke Busch, Stefanie Huegel, Gabin Sihn, Ilya Chuykin, Enno Hartmann, Michael Bader, Franziska Rother
Summary: The protein Kpna6 plays a crucial role in spermatogenesis and male fertility, with its deficiency leading to infertility and various effects on sperm and Sertoli cells. Its absence results in impaired Sertoli cell function, incomplete sperm maturation, and altered expression of genes involved in sperm production.
Article
Multidisciplinary Sciences
Raed Abu-Dawud, Bhavesh Mistry, Mohamed Rajab, Maha Alanazi, Nadya Al-Yacoub, Junaid Kashir, Falah Almohanna, Dilek Colak, Abdullah M. Assiri
Summary: This study found that disruption of Dcaf17 affects testicular protein homeostasis and transcriptional signature in mice, leading to male infertility.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Guohui Zhang, Chuan Jiang, Yushang Yang, Yan Wang, Haimeng Zhou, Siyu Dai, Mohan Liu, Yanting Yang, Li Yang, Qiongyan Shen, Tao Zhang, Xiaodong Zhang, Yihong Yang, Ying Shen
Summary: In this study, we reported male infertility patients with a hemizygous nonsense mutation in the cancer-testis antigen 55 (CT55) gene, which resulted in extreme disruption in sperm production, morphology, and locomotion. Our functional experiments revealed that CT55 acts as a novel autophagic manipulator involved in spermatogenesis.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Medicine, Research & Experimental
Zhihong Chen, Minjie Liu, Jin-Hua Hu, Yong Gao, Chunhua Deng, Mei Hua Jiang
Summary: The study demonstrates that the neuropeptide substance P plays a crucial role in spermatogonia proliferation and can potentially serve as a therapeutic agent for male infertility by accelerating the restoration of spermatogenesis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Manuel Gentiluomo, Alice Luddi, Annapaola Cingolani, Marco Fornili, Laura Governini, Ersilia Lucenteforte, Laura Baglietto, Paola Piomboni, Daniele Campa
Summary: The study found no significant association between sperm telomere length (STL) and leukocyte telomere length (LTL) with sperm quality parameters, but identified five single nucleotide polymorphisms (SNPs) that were statistically significantly associated with sperm variables. The results suggest moderate involvement of telomere length in male fertility, while indicating the potential pleiotropic effects of certain SNPs on sperm parameters independent of telomere homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chih-Wei Tsao, Pei-Shan Ke, Hsin-Yi Yang, Ting-Chia Chang, Chin-Yu Liu
Summary: This study aimed to investigate the effects of curcumin supplementation on male fertility issues induced by a low-carbohydrate diet. The results showed that replacing the low-carbohydrate diet with a normal diet or a ketogenic diet supplemented with curcumin improved testicular biopsy score and sperm morphology, while reducing oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Jose Luiz P. R. Jivago, Juliana Lis Mendes Brito, Gustavo Capistrano, Marcus Vinicius-Araujo, Ediron Lima Verde, Andris Figueiroa Bakuzis, Paulo E. N. Souza, Ricardo Bentes Azevedo, Carolina Madeira Lucci
Summary: The study found that testicular MNH is highly effective as a non-surgical sterilization method for animals, causing progressive seminiferous tubule degeneration followed by substitution of the parenchyma with stromal tissue and gonadal atrophy, suggesting an irreversible process with few side effects to general animal health.
Article
Endocrinology & Metabolism
Vincenza Precone, Rossella Cannarella, Stefano Paolacci, Gian Maria Busetto, Tommaso Beccari, Liborio Stuppia, Gerolamo Tonini, Alessandra Zulian, Giuseppe Marceddu, Aldo E. Calogero, Matteo Bertelli
Summary: This study utilized a next-generation sequencing gene panel to identify potential gene variants in idiopathic male infertile patients. The findings suggest that searching for pre-diagnostic genes may help in pinpointing the cause of idiopathic male infertility.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Andrology
Yoshitaka Fujihara, Kiyonori Kobayashi, Ferheen Abbasi, Tsutomu Endo, Zhifeng Yu, Masahito Ikawa, Martin M. Matzuk
Summary: In this study, Pdcl2 knockout mice were generated to investigate the essential roles of PDCL2 in mammalian reproduction. The findings showed that PDCL2 is crucial for sperm acrosome development and male fertility, suggesting it as a potential contraceptive target in men.
Article
Biology
Anna Keppner, Miguel Correia, Sara Santambrogio, Teng Wei Koay, Darko Maric, Carina Osterhof, Denise Winter, Angele Clerc, Michael Stumpe, Frederic Chalmel, Sylvia Dewilde, Alex Odermatt, Dieter Kressler, Thomas Hankeln, Roland H. Wenger, David Hoogewijs
Summary: Spermatogenesis relies on the gene expression program driven by Adgb, where the absence of Adgb leads to male infertility and abnormal sperm shape. Adgb interacts with Sept10, and the absence of Adgb mislocalizes Sept10 in sperm, revealing the importance of Adgb in proper sperm head shaping and flagellum formation.
Article
Cell Biology
Zhang Qian, Chuwei Li, Shanmeizi Zhao, Hong Zhang, Rujun Ma, Xie Ge, Jun Jing, Li Chen, Jinzhao Ma, Yang Yang, Lu Zheng, Kemei Zhang, Zhaowanyue He, Mengqi Xue, Ying Lin, Kadiliya Jueraitetibaike, Yuming Feng, Chun Cao, Ting Tang, Shanshan Sun, Hui Teng, Wei Zhao, Bing Yao
Summary: Postponing childbearing has become an important social issue, as male fertility is negatively affected by age-related testis aging. This study investigates the relationship between O-GlcNAc alteration and spermatogenesis during aging. The results demonstrate that the decline in spermatogenesis in aged mice is associated with elevated O-GlcNAc levels. Mechanistically, elevated O-GlcNAc leads to meiotic pachytene arrest, causing defects in synapsis and recombination. Lowering O-GlcNAc levels partially rescues age-related impairment of spermatogenesis. These findings highlight the role of O-GlcNAc in meiotic progression and its impact on spermatogenesis during aging.
CELL DEATH DISCOVERY
(2023)
Article
Cell Biology
Mohammad Afzal Khan, Ghazi Abdulmalik Ashoor, Talal Shamma, Fatimah Alanazi, Abdullah Altuhami, Shadab Kazmi, Hala Abdalrahman Ahmed, Abdullah Mohammed Assiri, Dieter Clemens Broering
Summary: Interleukin-10 plays a crucial role in maintaining immunotolerance and promoting graft microvascular repair and fibrosis suppression. IL-10 depletion exacerbates airway epithelial injury and fibrosis, while IL-10 reconstitution facilitates airway epithelial repair and fibrosis suppression.
Article
Oncology
Minjing Zou, Huda A. BinEssa, Yousif H. Al-Malki, Suhad Al-Yahya, Monther Al-Alwan, Ibrahim Al-Jammaz, Khalid S. A. Khabar, Falah Almohanna, Abdullah M. Assiri, Brian F. Meyer, Ali S. Alzahrani, Futwan A. Al-Mohanna, Yufei Shi
Summary: The study reveals that beta-catenin signaling is crucial in thyroid cancer growth and resistance to BRAF(V600E) inhibitors. Simultaneously targeting both Wnt/beta-catenin and MAPK signaling pathways may achieve better therapeutic outcome in BRAF(V600E) inhibitor-resistant and/or radioiodine-refractory thyroid cancer.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Wan-Sheng Liu, Chen Lu, Bhavesh V. Mistry
Summary: PRAME proteins, encoded by the PRAME gene family, are involved not only in transcriptional regulation in the nucleus but may also participate in nucleocytoplasmic transport and in the formation and function of germ cell-specific organelles during spermatogenesis.
CELL AND BIOSCIENCE
(2021)
Article
Biology
Nadya Al-Yacoub, Dilek Colak, Salma Awad Mahmoud, Maya Hammonds, Kunhi Muhammed, Olfat Al-Harazi, Abdullah M. Assiri, Jehad Al-Buraiki, Waleed Al-Habeeb, Coralie Poizat
Summary: The study reveals that the FBXO32 mutation-induced dilated cardiomyopathy is characterized by ER stress, abnormal CHOP activation, and CHOP-induced apoptosis in the absence of UPR effector activation, suggesting an alternative pathway for developing new therapeutic targets.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shadab Kazmi, Mohammad Afzal Khan, Talal Shamma, Abdullah Altuhami, Hala Abdalrahman Ahmed, Abdullah Mohammed Assiri, Dieter Clemens Broering
Summary: This study demonstrates that IL-10 can promote microvascular and epithelial repair in airway transplantation by regulating the expression of FOXJ1, beta-catenin, and Fascin-1 proteins. These findings provide an important therapeutic strategy for graft repair using IL-10.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Shadab Kazmi, Mohammad Afzal Khan, Talal Shamma, Abdullah Altuhami, Abdullah Mohammed Assiri, Dieter Clemens Broering
Summary: Immunometabolism is a therapeutic strategy that uses metabolic reprogramming to tune immune cells and treat inflammatory diseases, particularly those associated with organ transplantation. This review discusses how targeting T cell immunometabolism can modulate immune cell differentiation and activity, ultimately promoting immunotolerance in clinical transplants.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Mohammad Afzal Khan, Talal Shamma, Abdullah Altuhami, Hala Abdalrahman Ahmed, Abdullah Mohammed Assiri, Dieter Clemens Broering
Summary: This study demonstrates the important role of CTLA4-Ig mediated immunosuppression in preserving graft microvasculature and rescuing graft from sustained hypoxia and ischemia. It modulates both immune response and a late surge of regulatory activity, thus promoting graft repair and preventing progression of subepithelial fibrosis.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Raed Abu-Dawud, Bhavesh Mistry, Mohamed Rajab, Maha Alanazi, Nadya Al-Yacoub, Junaid Kashir, Falah Almohanna, Dilek Colak, Abdullah M. Assiri
Summary: This study found that disruption of Dcaf17 affects testicular protein homeostasis and transcriptional signature in mice, leading to male infertility.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Junaid Kashir, Bhavesh V. V. Mistry, Lujain BuSaleh, Michail Nomikos, Sarah Almuqayyil, Raed Abu-Dawud, Nadya AlYacoub, Hamdan Hamdan, Saad AlHassan, F. Anthony Lai, Abdullah M. M. Assiri, Serdar Coskun
Summary: The application of antigen unmasking/retrieval (AUM) enhances the visualization of PLC zeta in human sperm, allowing for accurate assessment of sperm fertilization capacity.
Article
Surgery
Aziza A. Ajlan, Hassan Aleid, Tariq Ali, Edward DeVol, Kris Ann Hervera Marquez, Rezqah Aldhaferi, Enaam Magzoub Karar, Amir Eltayeb Ismail Mohammed, Majid Tiba, Layal Fajji, Haifa Aldakhil, Moheeb Al-Awwami, Maha Saud Almslam, Abdallah Assiri, Talal Algoufi, Dieter C. Broering
Summary: This is an observational study on the safety and efficacy of the COVID-19 vaccine in pediatric kidney transplant recipients. The results showed that the vaccine was safe and effective in 12- to 15-year-old kidney transplant recipients, producing a higher antibody response.
CLINICAL TRANSPLANTATION
(2023)
Review
Biotechnology & Applied Microbiology
Tanveer Ahmed Mir, Alaa Alzhrani, Makoto Nakamura, Shintaroh Iwanaga, Shadil Ibrahim Wani, Abdullah Altuhami, Shadab Kazmi, Kenchi Arai, Talal Shamma, Dalia A. Obeid, Abdullah M. Assiri, Dieter C. Broering
Summary: Biomaterial templates play a critical role in establishing and instructing three-dimensional cellular growth in vitro liver models. Decellularized liver extracellular matrix (dECM) derived from xenogeneic or allogeneic sources is a promising natural biomaterial for the reconstruction of liver-specific whole organ scaffolds. This review discusses the latest updates in liver decellularization-recellularization technologies and highlights the potential of acellular matrix as a promising biomaterial.
BIOENGINEERING-BASEL
(2023)
Article
Andrology
Junaid Kashir, Bhavesh Mistry, Maha Adel Gumssani, Muhammad Rajab, Reema Abu-Dawas, Falah AlMohanna, Michail Nomikos, Celine Jones, Raed Abu-Dawud, Nadya Al-Yacoub, Kevin Coward, F. Anthony Lai, Abdullah M. Assiri
Summary: The study shows that with advancing male age, levels and variability of PLC zeta protein and mRNA in sperm decrease. There are also changes in the localization patterns of PLC zeta in sperm from older male mice.
ASIAN JOURNAL OF ANDROLOGY
(2021)