Article
Multidisciplinary Sciences
Joao Vasco Ferreira, Ana da Rosa Soares, Jose Ramalho, Catarina Maximo Carvalho, Maria Helena Cardoso, Petra Pintado, Ana Sofia Carvalho, Hans Christian Beck, Rune Matthiesen, Monica Zuzarte, Henrique Girao, Guillaume van Niel, Paulo Pereira
Summary: This article describes a mechanism in which proteins containing a KFERQ motif are loaded into a specific type of exosomes dependent on the membrane protein LAMP2A. This mechanism facilitates the transfer of hypoxia signaling to normoxic cells and provides a method for interorgan transfer of exosomes by tagging proteins with KFERQ-like sequences.
Article
Cell Biology
Nea Korvenlaita, Mireia Gomez-Budia, Flavia Scoyni, Cristiana Pistono, Luca Giudice, Shaila Eamen, Sanna Loppi, Ana Hernandez de Sande, Benjamin Huremagic, Maria Bouvy-Liivrand, Merja Heinaniemi, Minna U. Kaikkonen, Lesley Cheng, Andrew F. Hill, Katja M. Kanninen, Guido W. Jenster, Martin E. van Royen, Laura Ramiro, Joan Montaner, Tereza Batkova, Robert Mikulik, Rosalba Giugno, Jukka Jolkkonen, Paula Korhonen, Tarja Malm
Summary: Hypoxia induces changes in the secretion of extracellular vesicles (EVs) in non-neuronal cells and pathological conditions. The secretion of miR-21-5p in EVs is increased under hypoxia. Neuronal EVs and miR-21-5p secretion may be altered under hypoxia, and miR-21-5p in EVs may serve as a potential biomarker for stroke recovery.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Medicine, Research & Experimental
Huina Zhang, Lu Peng, Yifan Wang, Wen Zhao, Wayne Bond Lau, Yajing Wang, Yu Li, Yunhui Du, Linyi Li, Yu Huang, Shaoping Nie, Yanwen Qin, Xinliang Ma, Yongxiang Wei
Summary: This study reveals the role of circulating extracellular vesicles (EVs) in regulating endothelial function through the delivery of miR-144 to endothelial cells, reducing the expression of nuclear factor erythroid 2-related factor 2. Erythrocytes were identified as the primary cellular source of miR-144-enriched serum-derived EVs, which were largely responsible for endothelial dysfunction induced by chronic intermittent hypoxia. Silencing miR-144 confirmed its essential role in this dysfunction. The findings expand our understanding of blood-borne substances involved in vascular homeostasis and suggest that anti-miR-144-loaded EVs could be a promising therapeutic approach for conditions such as obstructive sleep apnea or chronic intermittent hypoxia-induced endothelial dysfunction.
Article
Biochemical Research Methods
Mahyar Dahmardeh, Houman Mirzaalian Dastjerdi, Hisham Mazal, Harald Koestler, Vahid Sandoghdar
Summary: An unsupervised machine learning approach improves the mass sensitivity of iSCAT by a factor of 4 to below 10 kDa, enabling the detection of small traces of biomolecules and disease markers.
Article
Biophysics
Tae Joon Kwak, Taehwang Son, Jae-Sang Hong, Ursula Andrea Winter, Mi Ho Jeong, Charlotte Mclean, Ralph Weissleder, Hakho Lee, Cesar M. Castro, Hyungsoon Im
Summary: Analysis of rare circulating extracellular vesicles (EVs) from early cancers or different types of host cells requires highly sensitive EV sensing technologies. In this study, an advanced plasmonic EV platform with electrokinetically enhanced yields (KeyPLEX) was developed, which overcame the diffusion limitation for specific target EV capture using applied electroosmosis and dielectrophoresis forces. The KeyPLEX system showed significant improvement in detection sensitivity, enabling sensitive detection of rare cancer EVs from human plasma samples within 10 minutes. The KeyPLEX system could be a valuable tool for point-of-care rapid EV analysis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Adam P. S. Bennett, Eduardo de la Torre-Escudero, Susan S. E. Dermott, Lawrence T. Threadgold, Robert E. B. Hanna, Mark W. Robinson
Summary: This study used laser microdissection to analyze the proteome of the gastrodermal cells in the liver fluke. The results showed that these cells are the main source of secreted proteins and may release extracellular vesicles. The study also highlighted the importance of the gastrodermal cells in nutrient acquisition and immunomodulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Zihao Ou, Xiaojing He, Qianbei Li, Nannan Cao, Meng Gao, Bairong He, Minying Zhang, Fang Hu, Weirong Yao, Qian Wang, Lei Zheng, Bo Situ
Summary: This study reports the development of a new bioprobe, DPASP, for imaging bacterial extracellular vesicles (BEVs). The bioprobe can effectively label and track BEVs through its aggregation-induced emission (AIE) attribute, and outperforms commercial dyes in terms of labeling efficiency, retention time, photostability, and biocompatibility.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biochemical Research Methods
Nan Lu, Hui Min Tay, Chayakorn Petchakup, Linwei He, Lingyan Gong, Kay Khine Maw, Sheng Yuan Leong, Wan Wei Lok, Hong Boon Ong, Ruya Guo, King Ho Holden Li, Han Wei Hou
Summary: Blood tests are standard clinical procedures for disease and health condition screening, but the complex cellular background and biomolecular composition often make accurate analysis challenging. Label-free microfluidic technologies that rely on intrinsic cell properties are emerging as a promising approach for point-of-care blood diagnostics. This review summarizes recent advances in label-free blood cell separation, single cell analysis, and the combination of microfluidics with machine learning for enhanced clinical studies.
Article
Gastroenterology & Hepatology
Na Sun, Ceng Zhang, Yi-Te Lee, Benjamin V. Tran, Jing Wang, Hyoyong Kim, Junseok Lee, Ryan Y. Zhang, Jasmine J. Wang, Junhui Hu, Zhicheng Zhang, Manaf S. Alsudaney, Kuan-Chu Hou, Hubert Tang, Tiffany X. Zhang, Icy Y. Liang, Ziang Zhou, Mengxiang Chen, Angela Hsiao-Jiun Yeh, Wenyuan Li, Xianghong Jasmine Zhou, Helena R. Chang, Steven-Huy B. Han, Saeed Sadeghi, Richard S. Finn, Sammy Saab, Ronald W. Busuttil, Mazen Noureddin, Walid S. Ayoub, Alexander Kuo, Vinay Sundaram, Buraq Al-Ghaieb, Juvelyn Palomique, Kambiz Kosari, Irene K. Kim, Tsuyoshi Todo, Nicholas N. Nissen, Maria Lauda Tomasi, Sungyong You, Edwin M. Posadas, James X. Wu, Madhuri Wadehra, Myung-Shin Sim, Yunfeng Li, Hanlin L. Wang, Samuel W. French, Shelly C. Lu, Lily Wu, Renjun Pei, Li Liang, Ju Dong Yang, Vatche G. Agopian, Hsian-Rong Tseng, Yazhen Zhu
Summary: The study developed an HCC EV-based surface protein assay for early detection of HCC, showing promising potential in detecting early-stage HCC.
Article
Biochemistry & Molecular Biology
Fumiya Ito, Katsuhiro Kato, Izumi Yanatori, Toyoaki Murohara, Shinya Toyokuni
Summary: The study proposed a novel mechanism in which extracellular vesicles mediate the transfer of mutagenic factors associated with asbestos to mesothelial cells. It was found that FedEVs transport iron from ferroptotic macrophages to mesothelial cells, contributing to asbestos-induced mesothelioma. Mesothelial cells receiving higher amounts of FedEVs were found to be in mitotic phases, particularly in S and G2/M phases.
Article
Chemistry, Analytical
Xiaokun Wang, Hezhen Shang, Cuiping Ma, Lingxin Chen
Summary: A fluorescence assay based on immunomagnetic separation and a two-step signal amplification strategy has been developed for sensitive and selective detection of exosomes. The method allows for the isolation and quantitative analysis of exosomes in biological fluids with satisfactory recovery rates, providing a sensitive and anti-interference platform for exosome isolation and detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Marcela A. Cucher, Maria Eugenia Ancarola, Laura Kamenetzky
Summary: In recent years, cell free or extracellular RNAs (ex-RNAs) have emerged as novel intercellular messengers between animal cells, including pathogens. They have been found to play important roles in infectious diseases and have been studied in parasitic helminths. Protected by encapsulation in extracellular vesicles (EV) or association to proteins or lipoproteins, ex-RNAs have been detected in the excretion/secretion products of helminth parasites.
MOLECULAR IMMUNOLOGY
(2021)
Article
Cell Biology
Hee Cheol Yang, Yoo Min Ham, Jeong Ah Kim, Won Jong Rhee
Summary: A novel single-step, equipment-free EV concentration method using super absorbent polymer (SAP) beads was developed in this study, which efficiently concentrates EVs while maintaining high purity of the EV solution. The method was shown to successfully enrich EVs in human urine samples and culture medium, outperforming conventional ultrafiltration methods.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Cell Biology
Yiqing Xu, Yulong Ge, Xuming Chen, Yingzi Zhang, Huanliang Chen, Dongli Liu, Yue Lu, Yong Liu, Wenzhi Tu
Summary: This study investigated the bystander effect of hypoxia on radiation-induced rectal injury. The results showed that secreted factors from hypoxic HIEC cells promoted apoptosis in normoxic HIEC cells. Further analysis revealed that miR-122-5p played a critical role in radiation-induced rectal injury. This study provides potential therapeutic targets for the treatment of radiation-induced rectal injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Haitao Zhang, Yan Shen, Il-man Kim, Yutao Liu, Jingwen Cai, Adam E. E. Berman, Kent R. R. Nilsson, Neal L. L. Weintraub, Yaoliang Tang
Summary: Clinical trials have shown that electric stimulation using cardiac resynchronization therapy or cardiac contractility modulation is effective for treating moderate to severe heart failure. This study investigated the effects of electric stimulation on the secretion of extracellular vesicles (EV) from cardiac mesenchymal stem cells (C-MSC). The results showed that electric stimulation enhanced EV-dependent cardioprotection and involved the enzyme nSMase2 in the endosomal sorting complex for EV biosynthesis.
Article
Mechanics
Alessandro Bongarzone, Arnaud Bertsch, Philippe Renaud, Francois Gallaire
Summary: The study investigates self-sustained oscillations induced by the interaction of two impinging jets in microfluidic devices, confirming a predominantly two-dimensional physical mechanism. Utilizing global stability analysis and weakly nonlinear theory, the research reveals hysteresis in the system within a certain aspect ratio range. The Kelvin-Helmholtz instability is identified as the main candidate for the observed oscillations in fluidic devices.
JOURNAL OF FLUID MECHANICS
(2021)
Article
Chemistry, Multidisciplinary
Tugba Kilic, Isabel Gessner, Young Kwan Cho, Naebong Jeong, Jeremy Quintana, Ralph Weissleder, Hakho Lee
Summary: A new polymeric material called zwitterionic polypyrrole (ZiPPy) is reported to have optimal surface condition for biosensing electrodes. ZiPPy combines the advantages of efficient hydration of the electrode surface and rapid deposition through electropolymerization. The ZiPPy-coated electrodes show lower electrochemical impedance and less nonspecific protein adsorption, outperforming other types of electrodes. Moreover, affinity ligands can be immobilized together with ZiPPy in a single-step electropolymerization. The ZiPPy-coated electrodes specifically detect SARS-CoV-2 antibodies in human saliva without the need for sample purification or secondary labeling.
ADVANCED MATERIALS
(2022)
Article
Engineering, Electrical & Electronic
Massimo Angeletti, Philippe Renaud, Corrado Gargiulo
Summary: A new type of cooling substrate for future High Energy Physics detectors is presented in this research. By using microchannels and a modular interlocking concept, the mechanical and hydraulic interconnection problem is solved, and the plug-and-play, interchangeability, and sealing performance of the substrates are improved.
MICROELECTRONIC ENGINEERING
(2022)
Article
Biochemical Research Methods
Heloise Hannart, Audrey Berger, Luc Aeberli, David Forchelet, Nicolas Uffer, Georges Muller, Yann Barrandon, Philippe Renaud, David Bonzon
Summary: Single-cell isolation is a transformative tool for understanding biological systems and has important implications for diagnosis, treatment, and personalized medicine. This study presents the transition from impedance-based pipetting technology into an automated instrument, DispenCell-S1, for traceable isolation of single cells. Models predicting the performance of single-cell isolation in 96-well plates were developed and validated, resulting in fast and efficient isolation with high reliability.
Article
Chemistry, Analytical
Joan Teixidor, Salvatore Novello, Daniel Ortiz, Laure Menin, Hilal A. Lashuel, Arnaud Bertsch, Philippe Renaud
Summary: The paper introduces a new Droplet on Demand (DoD) sampling method for collecting molecules from brain extracellular fluid in mice, allowing fast and accurate glucose quantification without transient regimes. This technique has the potential to be valuable for research on neurodegenerative diseases.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Clementine Lipp, Laure Koebel, Arnaud Bertsch, Michael Gauthier, Aude Bolopion, Philippe Renaud
Summary: We present a microfluidic dielectrophoretic-actuated system for trapping single cells and forming controlled cell aggregates. We propose a novel method to characterize the efficiency of dielectrophoretic trapping, taking into account both flow speed and heat generated by the traps as limiting factors for safe cell manipulation. Experimental comparison of two original designs with different manufacturing processes identifies the most efficient design, which is validated by monitoring cell membrane integrity using fluorescence imaging. We suggest design rules for adapting the traps to multiple-cell trapping, and experimentally demonstrate the formation of controlled size and composition aggregates using two different cell types. This study provides a simple manufactured tool for the controlled manipulation of particles in the composition of multicellular assemblies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Physics, Multidisciplinary
Dorian Herle, Philippe Renaud, Olivier Parriaux
Summary: This study explores the reflection switching concept of a free-space wave impinging on an array of dielectric or semiconductor pillars. By splitting the pillars and creating low-index gaps between them, the reflection of the wave can be effectively canceled. The study also designs a gapless experimental model and a pillar structure with nanometer-thick low-index gaps, achieving a broad reflection maximum at a specific wavelength and no reflection peak over a wide wavelength range.
JOURNAL OF PHYSICS COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Shiva Taheri, Zahra Sadat Ghazali, Leila Montazeri, Fatemeh Ale Ebrahim, Jafar Javadpour, Khorshid Kamguyan, Esben Thormann, Philippe Renaud, Shahin Bonakdar
Summary: The effect of cell imprinted substrates on the differentiation of ADSCs was investigated. The results showed that cell imprinted substrates can induce differentiation signals better than engineered lithographic substrates. Therefore, the patterns must include nano-topographies to have reliable and reproducible engineered substrates.
BIOMEDICAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Fatemeh Navaee, Philippe Renaud, Alexander Kleger, Thomas Braschler
Summary: Biochemical and biophysical properties of cardiac tissue can be manipulated by using a blend of decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen, which has shown enhanced cardiogenic differentiation and beating motion in a 3D cardiac cell culture model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Fluids & Plasmas
Jiande Zhou, Yves-Marie Ducimetiere, Daniel Migliozzi, Ludovic Keiser, Arnaud Bertsch, Francois Gallaire, Philippe Renaud
Summary: This work reports a novel breakup regime in the widely studied T-junction geometry, where the pinch-off occurs laterally in the two outlet channels, leading to the formation of three daughter droplets. It is driven by surface tension, and a design rule for the T-junction geometry is proposed. A model is developed to predict the formation and growth of an underlying carrier fluid pocket that accounts for this lateral breakup mechanism.
PHYSICAL REVIEW FLUIDS
(2023)
Article
Cell Biology
Fatemeh Navaee, Niloofar Khornian, David Longet, Sarah Heub, Stephanie Boder-Pasche, Gilles Weder, Alexander Kleger, Philippe Renaud, Thomas Braschler
Summary: Alignment of cardiomyocytes in myocardium tissue is important for the functions of the myocardium. This paper proposes a simple method to align cells in a 3D in vitro heart model using microfabricated PDMS grooves. The functionality of the model was evaluated by comparing beating rates between aligned and non-aligned structures. This model has potential applications in drug screening, tissue engineering, and heart-on-chip studies.
Article
Biotechnology & Applied Microbiology
Fatemeh Navaee, Philippe Renaud, Niccolo Piacentini, Mathilde Durand, Dara Zaman Bayat, Diane Ledroit, Sarah Heub, Stephanie Boder-Pasche, Alexander Kleger, Thomas Braschler, Gilles Weder
Summary: Myocardium is composed of cardiac cells that interact with their environment and their structure affects the physiology, function, and metabolism of cardiac tissue. This study aims to understand the mechanism of generating off-axis alignments in the myocardium and develop a reliable in vitro cardiac model. By applying cyclic mechanical stimulation and groove constraint, the researchers were able to engineer off-axis alignments similar to the helical arrangements observed in vivo. The study also shows that cyclical stretching supports cell survival and development, improving the model's function.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Analytical
Nicolas Maino, Arnaud Bertsch, Philippe Renaud
Summary: For more than 30 years, carbon fiber microelectrodes have been the gold standard for measuring processes at the synaptic level. However, these microelectrodes have limitations in terms of low throughput and detecting molecules due to their featureless nature. In this study, a new electrochemical sensor with insulated protruding volcano-shaped tips is introduced, which allows confined and parallel recordings of exocytosis from adherent cells. The sensor reveals faster exocytosis kinetics, potentially due to the perturbation of the plasma membrane by the sensor's topography, suggesting that substrate geometry can manipulate exocytosis kinetics and open up new avenues for studying synaptic processes.
Article
Biochemical Research Methods
Jiande Zhou, Amaury Wei, Arnaud Bertsch, Philippe Renaud
Summary: The Poisson limit poses a major challenge for isolating single cells, but this article presents a passive microfluidic system that overcomes this limitation with high efficiency. It has a high throughput and low fault ratio, making it suitable for various cells and particles.
Review
Biochemical Research Methods
David P. Taylor, Prerit Mathur, Philippe Renaud, Govind Kaigala
Summary: This review presents methods of using hydrodynamic phenomena to confine biological and chemical species, discussing their utility in life-sciences research. By choosing specific flow regimes and boundary conditions, hydrodynamic confinements can be applied to various functionalities, from cell separation and sorting to bio-printing and clinical diagnosis.
