Article
Public, Environmental & Occupational Health
Gary Crispell, Kelly Williams, Eric Zielinski, Akira Iwami, Zachary Homas, Karen Thomas
Summary: This study used multiple methods to detect Japanese encephalitis virus (JEV) in mosquito samples collected in South Korea and compared their effectiveness. The results showed that RT-PCR, Twist CVRP, and SISPA methods were able to detect JEV genotype I, while the proprietary Magpix panel could only detect JEV genotype III. The study found that RT-PCR was the most cost-effective method, but TWIST CVRP allowed for the identification of over 3,100 viral genomes.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Corey Nelson, Tyler Mrozowich, Darren L. Gemmill, Sean M. Park, Trushar R. Patel
Summary: The study demonstrates that DEAD-box helicase DDX3X can directly interact with and unwind flaviviral TRs in vitro. Therefore, DDX3X may be further explored as a therapeutic target to inhibit Flaviviral replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Takuma Izumi, Yuhei Morioka, Syun-ichi Urayama, Daisuke Motooka, Tomokazu Tamura, Takahiro Kawagishi, Yuta Kanai, Takeshi Kobayashi, Chikako Ono, Akinari Morinaga, Takahiro Tomiyama, Norifumi Iseda, Yukiko Kosai, Shoichi Inokuchi, Shota Nakamura, Tomohisa Tanaka, Kohji Moriishi, Hiroaki Kariwa, Tomoharu Yoshizumi, Masaki Mori, Yoshiharu Matsuura, Takasuke Fukuhara
Summary: A new method for efficient detection of RNA viruses in human clinical samples was established, which showed higher viral read ratio compared to conventional methods and correctly identified viral RNA. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases.
Review
Neurosciences
Xin Li, Jing Li, Guode Wu, Manxia Wang, Zhang Jing
Summary: Japanese encephalitis is an acute viral central nervous system disease with poor prognosis. Early detection and treatment are crucial. Metagenomic next-generation sequencing may be an effective method for rapid identification of the virus.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Microbiology
Qiang Xu, Lihong Huang, Jinchao Xing, Jiahao Zhang, Huanan Li, Lele Liu, Chen Hu, Ming Liao, Jianbo Yue, Wenbao Qi
Summary: The Japanese encephalitis virus manipulates cellular secretory pathways and autophagy components to create an optimal environment for genome replication. The virus replicates in lysosomes, not autophagosomes, and utilizes autophagic components for intracellular growth, shedding light on potential antiviral targets.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Seema R. R. Pegu, Pranab Jyoti Das, Joyshikh Sonowal, Gyanendra Singh Sengar, Rajib Deb, Ajay Kumar Yadav, Swaraj Rajkhowa, Manjisa Choudhury, Baldev R. R. Gulati, Vivek Kumar Gupta
Summary: This study conducted molecular detection and genome sequencing of Japanese encephalitis virus (JEV) isolates from pigs and mosquitoes in Assam, India. The analysis revealed that these isolates belong to genotype III and are closely related to strains from China, indicating the transboundary circulation of JEV genotype III through mosquito-swine transmission cycles.
Review
Virology
Baldeep Khare, Richard J. Kuhn
Summary: In the past three decades, multiple flaviviruses from different antigenic groups have spread geographically, leading to the co-circulation of multiple viruses within regions. The morphological heterogeneity of flaviviruses affects antibody recognition, virus neutralization, and infection control. Cross-reactivity among flaviviruses may result in either cross-protection or disease enhancement, although the molecular determinants and mechanisms driving these outcomes are still unclear.
Article
Immunology
Shuo Zhu, Mengying Tao, Yunchuan Li, Xugang Wang, Zikai Zhao, Yixin Liu, Qi Li, Qiuyan Li, Yanbo Lu, Youhui Si, Shengbo Cao, Jing Ye
Summary: This study found that infection with Japanese encephalitis virus (JEV) leads to an increase in H3K27me3 modification in microglial cells and mouse brain. Inhibiting H3K27me3 modification significantly reduces pro-inflammatory cytokine production during JEV infection, indicating its crucial role in the neuroinflammatory response. The increased H3K27me3 modification of E3 ubiquitin ligases following JEV infection leads to downregulation of Rnf19a expression and negative regulation of the neuroinflammatory response.
JOURNAL OF NEUROINFLAMMATION
(2023)
Review
Immunology
Weijia Zhang, Qikai Yin, Huanyu Wang, Guodong Liang
Summary: The Japanese encephalitis virus (JEV) has five genotypes, with genotypes 1 and 3 historically being more active. Genotypes 4 and 5 have remained silent in low-latitude tropical regions. However, in recent years, genotype 5 emerged in mosquitoes from Tibet and South Korea, leading to cases of viral encephalitis. Similarly, genotype 4 emerged in Australia, causing a local outbreak. These new genotypes present challenges for the prevention and control of Japanese encephalitis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Muddassar Hameed, Sawar Khan, Jinpeng Xu, Junjie Zhang, Xin Wang, Di Di, Zheng Chen, Muhammad Naveed Anwar, Abdul Wahaab, Xiaochun Ma, Mohsin Nawaz, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Jianchao Wei, Zhiyong Ma
Summary: Mosquito samples collected from animal farms near highly populated cities in Xinjiang showed detection of Japanese encephalitis virus, with Bayesian analysis suggesting a likely origin from China, emphasizing the need for further surveillance.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Chongxiao Xu, Weijia Zhang, Yuefeng Pan, Guowei Wang, Qikai Yin, Shihong Fu, Fan Li, Ying He, Songtao Xu, Zhenhai Wang, Guodong Liang, Kai Nie, Huanyu Wang
Summary: Japanese encephalitis (JE) is a mosquito-borne disease caused by the Japanese encephalitis virus (JEV). The research on JE has been ongoing for nearly 90 years, mainly in Asia. The analysis of English publications shows an increasing number of studies on JE, with a focus on the pathogenic mechanism of the virus, outbreak control, and immunization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Kumar Saurabh Srivastava, Vandana Jeswani, Nabanita Pal, Babita Bohra, Vaishali Vishwakarma, Atharva Ashish Bapat, Yamini Prashanti Patnaik, Navin Khanna, Rahul Shukla
Summary: Japanese encephalitis virus (JEV) is a potentially severe brain infection that spreads through mosquito bites. Currently, there is no licensed anti-JEV drug available, although there are a few licensed vaccines with limited global use. With over 67,000 cases of JE annually, there is an urgent need to find suitable antiviral drugs for treatment.
Article
Virology
Manjari Baluni, Sneha Ghildiyal, Tanzeem Fatima, Rashmi Tiwari, Shivbrat Upadhyay, Tapan N. Dhole, D. Himanshu Reddy, Dharamveer Singh
Summary: Japanese encephalitis (JE) is a serious vector-borne viral encephalitis disease that poses a major threat to public health. Research has found significantly altered expression of miR-155 and miR-21 in the serum of JEV-infected patients, indicating their potential as noninvasive biomarkers for the diagnosis of JE.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Biophysics
Akanksha Roberts, Veerbhan Kesarwani, Rupal Gupta, Sonu Gandhi
Summary: In this study, a Fluorine Doped Tin Oxide (FTO) electrode was fabricated with reduced Graphene Oxide (rGO) for sensitive detection of Japanese encephalitis virus (JEV) non-structural 1 (NS1) protein. The recombinant NS1 antigen and antibody were successfully cloned, expressed, characterized, and confirmed binding events through various spectroscopic and analytical techniques. The fabricated immunosensor showed high sensitivity, specificity, and rapid response for accurate and early diagnosis of JEV in clinical samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Claudia A. Rivera, Violaine Randrian, Wilfrid Richer, Yohan Gerber-Ferder, Maria-Graciela Delgado, Aleksandra S. Chikina, Annika Frede, Chiara Sorini, Mathieu Maurin, Hana Kammoun-Chaari, Sara M. Parigi, Christel Goudot, Mar Cabeza-Cabrerizo, Sylvain Baulande, Sonia Lameiras, Pierre Guermonprez, Caetano Reis e Sousa, Marc Lecuit, Helene D. Moreau, Julie Helft, Danijela Matic Vignjevic, Eduardo J. Villablanca, Ana-Maria Lennon-Dumenil
Summary: There are two pools of cDC2s in the small intestine, originating from common pre-DC precursors, and their phenotypes are influenced by food-derived retinoic acid.
Review
Immunology
Mathieu Blot, Olivier Disson, Alexandre Leclercq, Alexandra Moura, Helene Bracq-Dieye, Pierre Thouvenot, Guillaume Vales, Barbara Burroni, Audrey Lupo, Marc Lecuit, Caroline Charlier
Summary: The study found that Listeria-associated lymphadenitis is rare, but it is associated with a mortality rate as high as invasive listeriosis and is frequently diagnosed alongside neoplasia.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Review
Microbiology
Hana Kammoun, Minhee Kim, Lukas Hafner, Julien Gaillard, Olivier Disson, Marc Lecuit
Summary: In this review, we summarize some key steps of Lm-host interactions and highlight recent investigations using animal models and tagged strains. Studying Lm infection in vivo is a way forward to explore host biology and unveil the mechanisms that have selected its capacity to closely associate with its vertebrate hosts.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Editorial Material
Medicine, Research & Experimental
Laetitia Travier, Marc Lecuit
M S-MEDECINE SCIENCES
(2022)
Article
Infectious Diseases
Varanya Wasakul, Areeya Disratthakit, Mayfong Mayxay, Keobouphaphone Chindavongsa, Viengphone Sengsavath, Nguyen Thuy-Nhien, Richard D. Pearson, Sonexay Phalivong, Saiamphone Xayvanghang, Richard J. Maude, Sonia Goncalves, Nicholas P. Day, Paul N. Newton, Elizabeth A. Ashley, Dominic P. Kwiatkowski, Arjen M. Dondorp, Olivo Miotto
Summary: This study investigated a Plasmodium falciparum outbreak in Attapeu Province, Laos during the 2020-21 malaria season, and used genomic epidemiology methods to understand parasite population dynamics and its causes. The outbreak was driven by a selective sweep caused by rapid clonal expansion of a multidrug-resistant strain, resulting in a loss of genetic diversity. Genetic surveillance is important for characterizing outbreak features and supporting elimination efforts.
LANCET INFECTIOUS DISEASES
(2023)
Article
Immunology
Carla Palacios-Gorba, Alexandra Moura, Yuval Markovich, Nathalie Tessaud-Rita, Angel Gomez-Martin, Helene Bracq-Dieye, Jesus Gomis, Guillaume Vales, Maria Pastor-Martin, Pierre Thouvenot, Carles Escrig, Alexandre Leclercq, Marc Lecuit, Juan J. Quereda
Summary: Two species of pathogenic Listeria, L. monocytogenes and L. ivanovii, can asymptomatically colonize udders and tonsils of ruminants without fecal shedding. Their whole-genome sequences contain known core virulence and antibiotic resistance genes. This study highlights the importance of udders and tonsils as intra-host sites of Listeria colonization.
MICROBES AND INFECTION
(2023)
Article
Multidisciplinary Sciences
Richard Wheeler, Paulo Andre Dias Bastos, Olivier Disson, Aline Rifflet, Ilana Gabanyi, Julia Spielbauer, Marion Berard, Marc Lecuit, Ivo Gomperts Boneca
Summary: Peptidoglycan, the major component of bacterial cell walls, plays a crucial role in affecting physiological and behavioral changes in mammalian hosts. However, the mechanism and regulation of its translocation from the gut to host tissues have not been fully understood. This study investigated the absorption and dissemination of peptidoglycan across the intestine. The results showed that peptidoglycan has a preference for certain host organs, particularly the brain, when absorbed through the gut. The translocation of peptidoglycan is an active process regulated by the microbiota-induced parasympathetic pathway through muscarinic acetylcholine receptors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Public, Environmental & Occupational Health
Kristin Mullins, Enrique Canal, Pidor Ouch, Didot Prasetyo, Janice Tagoe, Naiki Attram, Clara Yeboah, Selassi Kumordjie, Anne Fox, Andrew G. G. Letizia, Audrey Rachlin, Hung Manh Nguyen, Matthew T. T. Robinson, Manivanh Vongsouvath, Viengmon Davong, Mayfong Maxay, Mark P. P. Simons, Angela Caranci, Paul N. N. Newton, Allen L. L. Richards, Christina M. M. Farris
Summary: This study screened residual sera from undifferentiated fever study participants in Cambodia, Ghana, Laos, and Peru to investigate human exposure to Bartonella species. Among 48 patients with suspected or confirmed Bartonella bacilliformis exposure or infection in Peru, 10 were Bartonella-specific IgG positive. Additionally, positive serology was found in samples from Peru, Laos, Cambodia, and Ghana. Fleas from Cambodia were found to carry Bartonella DNA, suggesting that fleas may serve as a vector for human exposure to Bartonella.
VECTOR-BORNE AND ZOONOTIC DISEASES
(2023)
Article
Virology
Geraldine Piorkowski, Jade Ghosn, Romain Coppee, Morgane Mailhe, Valentine Marie Ferre, Nadhira Houhou-Fidouh, Yazdan Yazdanpanah, Quentin Le Hingrat, Herve Raoul, Charlotte Charpentier, Diane Descamps, Xavier de Lamballerie
Summary: In May 2022, mpox cases were reported in several countries without a travel history to endemic areas. France experienced a significant outbreak, and this study analyzed the clinical characteristics and genetic diversity of mpox cases in the country. The study included mpox-infected patients diagnosed between May 21 and July 4, 2022, and between August 16 and September 10, 2022. Genetic diversity analysis revealed low variability compared to pre-epidemic Western-African sequences, with 32 distinct mutational patterns. This study provides valuable insights into the early 2022 circulating strains of mpox in Paris, France.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Medicine, General & Internal
George M. Varghese, Divya Dayanand, Karthik Gunasekaran, Debasree Kundu, Mukta Wyawahare, Navneet Sharma, Dhruva Chaudhry, Sanjay K. Mahajan, Kavitha Saravu, Blessed W. Aruldhas, Binu S. Mathew, Roshini G. Nair, Nalini Newbigging, Aswathy Mathew, Kundavaram P. P. Abhilash, Manisha Biswal, Ann H. Prasad, Anand Zachariah, Ramya Iyadurai, Samuel G. Hansdak, Sowmya Sathyendra, Thambu D. Sudarsanam, John A. J. Prakash, Abi Manesh, Alladi Mohan, Joel Tarning, Stuart D. Blacksell, Pimnara Peerawaranun, Naomi Waithira, Mavuto Mukaka, Phaik Yeong Cheah, John V. Peter, Ooriapadickal C. Abraham, Nicholas P. J. Day
Summary: This multicenter, double-blind, randomized, controlled trial compared the efficacy of intravenous doxycycline, azithromycin, or a combination of both for the treatment of severe scrub typhus. The results showed that combination therapy was more effective in reducing the occurrence of the primary outcome compared to monotherapy.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Microbiology
Peiqi Yin, Bennett J. Davenport, Judy J. Wan, Arthur S. Kim, Michael S. Diamond, Brian C. Ware, Karen Tong, Therese Couderc, Marc Lecuit, Jonathan R. Lai, Thomas E. Morrison, Margaret Kielian
Summary: The Chikungunya virus (CHIKV) forms long intercellular extensions that connect infected and neighboring cells, facilitating efficient transmission of the virus. These extensions are critical for CHIKV transmission, which occurs through endocytosis and endosomal acidification, rather than the CHIKV receptor MXRA8. In vivo experiments demonstrate that neutralizing antibodies are unable to block infection when the virus is transferred through infected cells. This study suggests that the contact sites of the intercellular extensions shield CHIKV from neutralizing antibodies, promoting efficient virus transmission both in vitro and in vivo.
NATURE MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Miguel Garcia-de la Virgen, Inmaculada Lopez-Almela, Alexandra Moura, Sergio Vazquez, Sara Perez-Montagud, Alexandre Leclercq, Marc Lecuit, Juan J. Quereda
Summary: This report describes a case of Listeria monocytogenes-associated mesenteric lymphadenitis in a cat, highlighting the importance of this syndrome for veterinary clinicians and microbiologists in terms of health and food safety for animals and humans.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Alice Decombe, Olve Peersen, Priscila Sutto-Ortiz, Celia Chamontin, Geraldine Piorkowski, Bruno Canard, Sebastien Nisole, Etienne Decroly
Summary: 2'-O-methylation modification of viral RNA genomes negatively affects reverse transcriptase activity, reducing nucleotide incorporation efficiency and impairing translocation. This study reveals the significance of RNA modification during viral genome replication.
NUCLEIC ACIDS RESEARCH
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Frouin Arthur, Fabien Laporte, Hafner Lukas, Maury Mylene, Marc Lecuit, Chikhi Rayan, Hugues Aschard
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Public, Environmental & Occupational Health
Jean David Pommier, Chris Gorman, Yoann Crabol, Kevin Bleakley, Heng Sothy, Ky Santy, Huong Thi Thu Tran, Lam Van Nguyen, Em Bunnakea, Chaw Su Hlaing, Aye Mya Min Aye, Julien Cappelle, Magali Herrant, Patrice Piola, Bruno Rosset, Veronique Chevalier, Arnaud Tarantola, Mey Channa, Jerome Honnorat, Anne Laure Pinto, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Sommanikhone Phangmanixay, Khounthavy Phongsavath, Ommar Swe Tin, Latt Latt Kyaw, Htay Htay Tin, Kyaw Linn, Thi Mai Hung Tran, Philippe Perot, Nguyen Thi Thu Thuy, Nguyen Hien, Phuc Huu Phan, Philippe Buchy, Philippe Dussart, Denis Laurent, Marc Eloit, Audrey Dubot-Peres, Olivier Lortholary, Xavier de Lamballerie, Paul N. Newton, Marc Lecuit
Summary: The study found that in Southeast Asia, most causes of childhood encephalitis are either preventable or treatable, with Japanese encephalitis virus being the most common cause. These results provide crucial information for improving diagnostic, vaccination, and early therapeutic guidelines for childhood encephalitis in the Greater Mekong region.
LANCET GLOBAL HEALTH
(2022)
