Article
Multidisciplinary Sciences
Teodora Manea, Jessica Kristine Nelson, Cristina Maria Garrone, Karin Hansson, Ian Evans, Axel Behrens, Rocio Sancho
Summary: Understanding the role of NGN3 in beta-cell development is crucial for developing therapies for diabetes. This study identifies USP7 as a key regulator of NGN3 stability and shows that it is necessary for endocrine development and beta-cell differentiation. Inhibition of USP7 reduces NGN3 expressing progenitor cell numbers and impairs beta-cell differentiation. The findings suggest that targeting the USP7-NGN3 axis could be a therapeutic strategy for generating functional beta cells.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Chiemi Kimura-Nakajima, Kousuke Sakaguchi, Yoshiko Hatano, Masahito Matsumoto, Yasushi Okazaki, Keisuke Tanaka, Takumi Yamane, Yuichi Oishi, Kenji Kamimoto, Ken Iwatsuki
Summary: The authors found through experimental research that Ngn3-positive endocrine precursor cells in adult mice may arise from the pancreatic duct, indicating that the pancreatic duct could be a potential donor for therapeutic use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Christopher Gribben, Christopher Lambert, Hendrik A. Messal, Ella-Louise Hubber, Chloe Rackham, Ian Evans, Harry Heimberg, Peter Jones, Rocio Sancho, Axel Behrens
Summary: Ductal cells have been identified as a source of adult beta cell neogenesis, contributing to the beta cell population over time. The expression of Ngn3 in ductal cells plays a role in adult beta cell neogenesis, potentially impacting diabetes onset. Additionally, single-cell RNA sequencing revealed duct-derived somatostatin-expressing cells gave rise to beta cells.
Article
Multidisciplinary Sciences
Hwee Hui Lau, Nicole A. J. Krentz, Fernando Abaitua, Marta Perez-Alcantara, Jun-Wei Chan, Jila Ajeian, Soumita Ghosh, Yunkyeong Lee, Jing Yang, Swaraj Thaman, Benoite Champon, Han Sun, Alokkumar Jha, Shawn Hoon, Nguan Soon Tan, Daphne Su-Lyn Gardner, Shih Ling Kao, E. Shyong Tai, Anna L. Gloyn, Adrian Kee Keong Teo
Summary: The coding variant of the PAX4 gene is associated with an altered risk of type 2 diabetes in East Asian populations. This study found that individuals carrying the specific allele of PAX4 exhibited decreased pancreatic beta cell function, and a newly identified allele was found in some diabetes patient pedigrees. Knockdown of the PAX4 gene resulted in impaired insulin secretion and altered hormone gene expression in a human beta cell model. In vitro differentiation experiments also showed that cells carrying specific risk alleles exhibited increased polyhormonal endocrine cell formation and decreased insulin content.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Natanya Kerper, Sudipta Ashe, Matthias Hebrok
Summary: This review highlights the importance of pancreatic beta-cells for regulating glucose homeostasis and discusses the potential of other cell types to acquire beta-cell functionality through transdifferentiation. It also explores human stem cell differentiation efforts to restore normoglycemia in patients with diabetes.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2022)
Article
Cell Biology
Minglei Zhao, Tingfang Mei, Bizhi Shang, Bin Zou, Qing Lian, Wenchang Xu, Keling Wu, Yuhua Lai, Chujun Liu, Lai Wei, Jie Zhu, Kang Zhang, Yizhi Liu, Ling Zhao
Summary: The study demonstrates that a mouse model carrying Lss G589S mutation can recapitulate human congenital cataract and indicates the critical role of LSS in lens development. The mutation leads to opacity in eye lens formation, disrupted lens differentiation, and downregulation of cholesterol synthesis signaling pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Francesca Marini, Francesca Giusti, Francesco Tonelli, Maria Luisa Brandi
Summary: Pancreatic neuroendocrine tumors (pNETs) are a rare group of cancers, accounting for about 1-2% of all pancreatic neoplasms, with some arising in patients with endocrine tumor syndromes such as Multiple Endocrine Neoplasia type 1 (MEN1). MEN1-related pNETs present challenges in treatment due to their unique characteristics and the need for extensive surgery. Epigenetic-based therapies aiming to correct DNA methylation and histone modifications may offer a potential therapeutic strategy for MEN1 pNETs resistant to standard treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Endocrinology & Metabolism
Giacomo Lanzoni, Camillo Ricordi
Summary: Many diabetes patients rely on insulin treatment to manage their glucose metabolism, but this approach comes with risks of hypoglycemia and suboptimal metabolic control. Stem cell-derived pancreatic islet cell transplantation could present a promising solution, inching closer towards clinical translation.
NATURE REVIEWS ENDOCRINOLOGY
(2021)
Article
Cell Biology
Hadley E. Sheppard, Alessandra Dall'Agnese, Woojun D. Park, M. Hamza Shamim, Julien Dubrulle, Hannah L. Johnson, Fabio Stossi, Patricia Cogswell, Josh Sommer, Joan Levy, Tanaz Sharifnia, Mathias J. Wawer, Behnam Nabet, Nathanael S. Gray, Paul A. Clemons, Stuart L. Schreiber, Paul Workman, Richard A. Young, Charles Y. Lin
Summary: Brachyury degradation disrupts its autoregulation, leading to loss of transcriptional program and sensitizing cells to anti-apoptotic inhibition, while transcriptional CDK inhibition globally downregulates transcription, leading to cell death.
CELL REPORTS MEDICINE
(2021)
Article
Environmental Sciences
Melanie K. Stewart, Luca Hoehne, Stefanie Dudczig, Deidre M. Mattiske, Andrew J. Pask, Patricia R. Jusuf
Summary: Endocrine disrupting chemicals (EDCs) can interfere and disrupt hormone signalling necessary for various developmental pathways by interacting with native hormone receptors. These chemicals are pervasive in our environment, particularly in our waterways, making aquatic wildlife highly vulnerable to their effects. Many EDCs can bind to and activate oestrogen receptors, leading to aberrant oestrogen signalling.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Biochemistry & Molecular Biology
Xiaoli Wang, Lisha Yin, Yujiao Wen, Shuiqiao Yuan
Summary: Mitochondria adjust their morphology to suit different cell types and environments, playing important roles in spermatogenesis. Deficiencies in mitochondria can cause male infertility and regulate piRNA biogenesis. This review describes the characteristics of mitochondria, focusing on key mitochondrial factors and the underlying molecular mechanisms in spermatogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Engineering, Biomedical
Ri Huang, Hong Du, Liang Cheng, Peizhuo Zhang, Fenghua Meng, Zhiyuan Zhong
Summary: In this study, cRGD peptide-modified bioresponsive chimaeric polymersomes (cRGD-BCP) were used for efficient delivery of siKRAS to PANC-1 tumor cells, resulting in potent silencing of KRAS G12D mRNA and effective suppression of pancreatic cancer tumor growth in mice. The density of cRGD greatly impacted the uptake and silencing efficiency of cRGD-BCP-siKRAS. cRGD-BCP-siKRAS significantly enhanced the uptake of siKRAS in PANC-1 tumor and achieved remarkable tumor inhibition and survival benefits.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Romana Bohuslavova, Valeria Fabriciova, Ondrej Smolik, Laura Lebron-Mora, Pavel Abaffy, Sarka Benesova, Daniel Zucha, Lukas Valihrach, Zuzana Berkova, Frantisek Saudek, Gabriela Pavlinkova
Summary: NEUROD1 is a transcription factor that plays an important role in pancreatic development and is crucial for the differentiation of endocrine cells. Its deficiency disrupts endocrine cell identity acquisition and compromises their differentiation and functional properties.
NATURE COMMUNICATIONS
(2023)
Article
Pediatrics
Gangaram Akangire, Heather Menden, Sheng Xia, Isabelle Thiffault, Atif Ahmed, Venkatesh Sampath
Summary: Nonimmune hydrops is associated with developmental anomalies of central lymphatic vessels. This study identified a rare EPHB4 mutation in an infant with severe neonatal hydrops, highlighting the importance of screening for mutations in EPHB4 and other genes that regulate lymphatic development in infants with nonimmune hydrops.
Article
Biochemistry & Molecular Biology
Romana Bohuslavova, Valeria Fabriciova, Laura Lebron-Mora, Jessica Malfatti, Ondrej Smolik, Lukas Valihrach, Sarka Benesova, Daniel Zucha, Zuzana Berkova, Frantisek Saudek, Sylvia M. Evans, Gabriela Pavlinkova
Summary: By using transgenic mouse models, transcriptomic and epigenomic profiling, we found that the absence of Isl1 leads to a diabetic phenotype, loss of alpha cells, disrupted pancreatic islet architecture, downregulation of key ss-cell regulators and maturation markers, and an enrichment in an intermediate endocrine progenitor transcriptomic profile.
CELL AND BIOSCIENCE
(2023)
Article
Cell Biology
Dong-Kyung Lee, Chi-Hun Park, Kwang-Hwan Choi, Yeon-ik Jeong, Kyung-Jun Uh, Jae Yeon Hwang, Sang-Goo Lee, Chang-Kyu Lee
Article
Biochemistry & Molecular Biology
Bhanu P. Telugu, Ki-Eun Park, Chi-Hun Park
Article
Multidisciplinary Sciences
Ki-Eun Park, Amy V. Kaucher, Anne Powell, Muhammad Salman Waqas, Shelley E. S. Sandmaier, Melissa J. Oatley, Chi-Hun Park, Ahmed Tibary, David M. Donovan, Le Ann Blomberg, Simon G. Lillico, Bruce A. Whitelaw, Alan Mileham, Bhanu P. Telugu, Jon M. Oatley
SCIENTIFIC REPORTS
(2017)
Article
Biotechnology & Applied Microbiology
Rosemarie W. Hammond, Steven M. Swift, Jul A. Foster-Frey, Natalia Y. Kovalskaya, David M. Donovan
Article
Multidisciplinary Sciences
Michela Ciccarelli, Mariana Giassetti, Deqiang Miao, Melissa J. Oatley, Colton Robbins, Blanca Lopez-Biladeau, Muhammad Salman Waqas, Ahmed Tibary, Bruce Whitelaw, Simon Lillico, Chi-Hun Park, Ki-Eun Park, Bhanu Telugu, Zhiqiang Fan, Ying Liu, Misha Regouski, Irina A. Polejaeva, Jon M. Oatley
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cell & Tissue Engineering
Chi-Hun Park, Young-Hee Jeoung, Kyung-Jun Uh, Ki-Eun Park, Jessica Bridge, Anne Powell, Jie Li, Laramie Pence, Luhui Zhang, Tianbin Liu, Hai-Xi Sun, Ying Gu, Yue Shen, Jun Wu, Juan-Carlos Izpisua Belmonte, Bhanu P. Telugu
Summary: This study details the characterization of pig extraembryonic endoderm (pXEN) cells and their applications in generating live offspring and genome editing in livestock.
Article
Cell Biology
Chi-Hun Park, Young-Hee Jeoung, Luhui Zhang, Sai Goutham Reddy Yeddula, Ki-Eun Park, Jerel Waters, Bhanu P. P. Telugu
Summary: EFs derived from porcine embryonic outgrowths exhibit similarities to FFs. EFs can be used as nuclear donors in SCNT to enhance post-blastocyst development, implantation, and pregnancy outcomes, and as a source for CRISPR/Cas genome editing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Infectious Diseases
Steven M. Swift, Kevin P. Reid, David M. Donovan, Timothy G. Ramsay
Article
Infectious Diseases
Steven M. Swift, Irina Etobayeva, Kevin P. Reid, Jerel J. Waters, Brian B. Oakley, David M. Donovan, Daniel C. Nelson
Article
Microbiology
Steven M. Swift, Jerel J. Waters, D. Treva Rowley, Brian B. Oakley, David M. Donovanl
FEMS MICROBIOLOGY LETTERS
(2018)
Review
Veterinary Sciences
Bruce S. Seal, Djamel Drider, Brian B. Oakley, Harald Brussow, David Bikard, Joseph O. Rich, Stefan Miller, Estelle Devillard, Jason Kwan, Gerard Bertin, Stuart Reeves, Steven M. Swift, Margot Raicek, Cyril G. Gay
VETERINARY RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Timothy P. Sheets, Chi-Hun Park, Ki-Eun Park, Anne Powell, David M. Donovan, Bhanu P. Telugu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)
Article
Biochemistry & Molecular Biology
Ki-Eun Park, Chi-Hun Park, Anne Powell, Jessica Martin, David M. Donovan, Bhanu P. Telugu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)