Article
Biochemistry & Molecular Biology
Ziyan Zhang, Ping Xu, Zhe Hu, Zhaojian Fu, Tongyuan Deng, Xiaojie Deng, Lanzhu Peng, Yingying Xie, Lingzhi Long, Dayong Zheng, Peng Shen, Mengmin Zhang, Bin Gong, Zhibo Zhu, Junhao Lin, Rui Chen, Zhen Liu, Huilin Yang, Rong Li, Weiyi Fang
Summary: CCDC65 is a potential tumor suppressor in lung adenocarcinoma, which can suppress tumor development by inhibiting the cell cycle signal induced by c-Myc/ENO1.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Dan Li, Suat Peng Neo, Jayantha Gunaratne, Kanaga Sabapathy
Summary: Polyamines promote cellular proliferation by controlling the levels of ornithine decarboxylase antizyme 1 (Az1), which degrades ornithine decarboxylase (ODC) responsible for polyamine biosynthesis. This study identified LIM domain and actin-binding protein 1 (LIMA1) as a new Az1 target, specifically the EPLIN-0 isoform. The absence of Az1 led to increased levels of EPLIN-0 and enhanced cellular migration, suggesting its role in regulating this process related to tumorigenesis.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
James L. Shen, Tina M. Fortier, Ruoxi Wang, Eric H. Baehrecke
Summary: Defects in autophagy can lead to issues in metabolism, development, and disease. The loss of Vps13D affects mitophagy, regulated by the core autophagy machinery. Pink1 and Vps13D play roles in Pink1-dependent mitophagy, with Park contributing to mitochondrial clearance through a pathway parallel to Vps13D.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Gun West, Minttu Turunen, Anna Aalto, Laura Virtanen, Song-Ping Li, Tiina Helio, Annika Meinander, Pekka Taimen
Summary: This study reveals the intricate interplay between mutant lamins A and C and the protein degradation machinery. Mutant lamins A and C reduce the activity of the ubiquitin-proteasome system (UPS), leading to accumulation of K48-ubiquitin chains. However, compensatory enhanced autophagy can degrade the mutant lamins A and C. Furthermore, pharmacological interventions that enhance protein degradation can be beneficial for maintaining cellular homeostasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Mingmin Tang, Xin Xie, Muran Shi, Wenxiu Xin, Guowan Zheng, Yanhua Zhang, Zhizhen Zhang, Xiaoyuan Lian
Summary: "CADPE shows potent activity against various leukemia cell lines by promoting the ubiquitin-proteasome-dependent degradation of c-Myc, resulting in cell cycle arrest and apoptosis by downregulating cell cycle regulators and anti-apoptotic proteins."
Article
Oncology
Sagar Bajpai, Hong Ri Jin, Bartosz Mucha, J. Alan Diehl
Summary: Overexpression of c-myc is a common feature in many cancer types, and its degradation is mediated by ubiquitin-dependent degradation. Fbxl8, a new E3 ligase, targets c-myc for selective degradation via the SCF ubiquitin ligase complex, independent of the primary regulator Fbxw7. Loss of Fbxl8 increases c-myc protein levels and cell division, while overexpression of Fbxl8 reduces c-myc protein levels.
CANCER BIOLOGY & THERAPY
(2022)
Article
Multidisciplinary Sciences
Giovanni Quarato, Luigi Mari, Nicholas J. Barrows, Mao Yang, Sebastian Ruehl, Mark J. Chen, Cliff S. Guy, Jonathan Low, Taosheng Chen, Douglas R. Green
Summary: The degradation of defective mitochondria is regulated by the ubiquitin-proteasome system and lysosomal activities, which play essential roles in maintaining cellular homeostasis. Activation of the PINK1-Parkin axis following mitochondrial damage triggers a BAX- and BAK-independent cytochrome c release process, leading to apoptosis mediated by APAF1 and caspase 9. This process is initiated by UPS-dependent outer mitochondrial membrane degradation and can be reversed by proteasome inhibitors. Autophagy machinery recruitment to the outer mitochondrial membrane protects cells from apoptosis by mediating the lysosomal degradation of dysfunctional mitochondria. The study highlights the major role of the autophagy machinery in counteracting abnormal noncanonical apoptosis and identifies autophagy receptors as key regulators of this process.
Article
Multidisciplinary Sciences
Anna S. Dickson, Tekle Pauzaite, Esther Arnaiz, Brian M. Ortmann, James A. West, Norbert Volkmar, Anthony W. Martinelli, Zhaoqi Li, Niek Wit, Dennis Vitkup, Arthur Kaser, Paul J. Lehner, James A. Nathan
Summary: Researchers have discovered an oxygen-sensitive pathway that regulates cholesterol synthesis in human cells, which involves the degradation of SREBP2 under hypoxic conditions. This pathway overrides the normal response to sterol levels and is independent of HIF. The degradation of SREBP2 in hypoxia protects cells from statin-induced cell death and leads to a dependence on exogenous cholesterol uptake, shedding light on the auxotrophic nature of many solid organ tumors for cholesterol.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Boyu Zhang, Qingxin Liu, Weijie Wen, Han Gao, Wenxia Wei, Aiwen Tang, Baifu Qin, Haiwen Lyu, Xiangqi Meng, Kai Li, Huilin Jin, Fenghai Yu, Qihao Pan, Junzhong Lin, Mong-Hong Lee
Summary: Chromodomain helicase DNA binding protein 6 (CHD6) is highly expressed in colorectal cancer and plays a critical role in regulating cancer cell proliferation and invasion through its stability and chromatin remodeling activity. The CHD6-TMEM65 axis is regulated by both Wnt and EGF signaling pathways, and targeting this axis may be effective for cancer intervention.
Article
Multidisciplinary Sciences
Donghong Ju, Li Li, Youming Xie
Summary: Ribosomal proteins are regulated by cotranslational protein degradation, which does not require prior ubiquitylation. Cotranslational folding efficiency and expression level are critical factors determining cotranslational protein degradation of ribosomal proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Virology
Lei Hou, Xiaohan Hu, Jinshuo Guo, Rong Quan, Li Wei, Jing Wang, Jiangwei Song, Jue Liu
Summary: In this study, we found that aMPV/C infection induces MAVS degradation through the ubiquitin-proteasome pathway at the post-translational level. Furthermore, specific amino acid sites were identified as pivotal in the formation of polyubiquitin chains during MAVS degradation. E3 ubiquitin ligases targeting MAVS were also identified, with RNF5 shown to be involved in MAVS degradation in aMPV/C-infected cells.
Article
Biochemistry & Molecular Biology
Liuqian Xu, Suning Che, Huiqing Chen, Qian Liu, Juanjuan Shi, Jianhua Jin, Yongzhong Hou
Summary: As a master transcription factor, c-Myc promotes tumor immune escape. The role of PPARγ in c-Myc-mediated tumor immune escape is unclear. This study found that the PPARγ agonist pioglitazone reduced c-Myc protein expression by inducing its ubiquitination and degradation. Additionally, pioglitazone inhibited the expression of immune checkpoint proteins PD-L1 and CD47, thus inhibiting tumor immune escape mediated by c-Myc.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wenxia Wei, Baifu Qin, Weijie Wen, Boyu Zhang, Haidan Luo, Yuzhi Wang, Hui Xu, Xiaoshan Xie, Sicheng Liu, Xin Jiang, Mengan Wang, Qin Tang, Jiayu Zhang, Runxiang Yang, Zongmin Fan, Haiwen Lyu, Junzhong Lin, Kai Li, Mong-Hong Lee
Summary: Continuous de novo fatty acid synthesis is essential for tumor growth. FBXW7 beta, an isoform of FBXW7 highly mutated in colorectal cancer (CRC), functions as an E3 ligase for fatty acid synthase (FASN). Mutations in FBXW7 beta, specific to CRC, result in sustained lipogenesis. The oncogenic marker CSN6 interacts with and stabilizes FASN, promoting lipogenesis and poor prognosis in CRC. The CSN6-FASN axis, regulated by EGF, contributes to tumor growth and can be targeted with combination therapy of orlistat and cetuximab for CSN6/FASN-high CRC.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Environmental Sciences
Runzhi Hu, Chenjuan Yao, Yanli Li, Jianhua Qu, Shali Yu, Yu Han, Gang Chen, Juan Tang, Haiyan Wei
Summary: Microplastics and nanoplastics can disrupt the blood-testis barrier, but the molecular mechanism is unclear. This study found that polystyrene nanoparticles degrade tight junction proteins in the testis by activating the IRE1α/XBP1s/CHIP pathway, leading to blood-testis barrier destruction. These findings reveal the potential harm of microplastics and nanoplastics to organisms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Abramo J. Manfredonia, Daniel A. Kraut
Summary: The ubiquitin-proteasome system is responsible for protein degradation in eukaryotic cells. The study showed that degradation of ubiquitin-independent degrons (UbIDs) is slower and relies on loosely folded substrates. Furthermore, UbID degradation is ATP-independent.
Article
Biochemistry & Molecular Biology
Ayasa Tajima, Noriyuki Murai, Yasuko Murakami, Takeo Iwamoto, Toshiro Migita, Senya Matsufuji
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Yasuko Murakami, Makiko Ohkido, Hiroko Takizawa, Noriyuki Murai, Senya Matsufuji
Article
Oncology
Yukihiro Hirata, Noriyuki Murai, Nozomu Yanaihara, Misato Saito, Motoaki Saito, Mitsuyoshi Urashima, Yasuko Murakami, Senya Matsufuji, Aikou Okamoto
Article
Biochemistry & Molecular Biology
Noriyuki Murai, Akihiro Shimizu, Yasuko Murakami, Senya Matsufuji
JOURNAL OF CELLULAR BIOCHEMISTRY
(2009)
Article
Urology & Nephrology
Keiichiro Matoba, Daiji Kawanami, Rina Okada, Masami Tsukamoto, Jun Kinoshita, Tomoko Ito, Sho Ishizawa, Yasushi Kanazawa, Tamotsu Yokota, Noriyuki Murai, Senya Matsufuji, Junko Takahashi-Fujigasaki, Kazunori Utsunomiya
KIDNEY INTERNATIONAL
(2013)
Article
Multidisciplinary Sciences
Satoshi Katagiri, Takaaki Hayashi, Kazutoshi Yoshitake, Noriyuki Murai, Zenichi Matsui, Hiroyuki Kubo, Hiroyuki Satoh, Senya Matsufuji, Tsuyoshi Takamura, Takashi Yokoo, Yoshihiro Omori, Takahisa Furukawa, Takeshi Iwata, Tadashi Nakano
SCIENTIFIC REPORTS
(2018)
Article
Ophthalmology
Satoshi Katagiri, Katsuhiro Hosono, Takaaki Hayashi, Noriyuki Murai, Eiichi Wake, Ichiro Miyata, Kei Mizobuchi, Kentaro Kurata, Tomokazu Matsuura, Tadashi Nakano, Yoshihiro Hotta
DOCUMENTA OPHTHALMOLOGICA
(2020)
Article
Cell Biology
Takahiko J. Fujmi, Yoshihiro Mezaki, Takahiro Masaki, Ayasa Tajima, Mariko Nakamura, Akira Yoshikawa, Noriyuki Murai, Mamoru Aizawa, Soichi Kojima, Yoshihiro Matsumoto, Hideki Aizaki, Tomokazu Matsuura
Article
Cell Biology
Keisuke Tamari, Masamitsu Konno, Ayumu Asai, Jun Koseki, Kazuhiko Hayashi, Koichi Kawamoto, Noriyuki Murai, Senya Matsufuji, Fumiaki Isohashi, Taroh Satoh, Noriko Goto, Shinji Tanaka, Yuichiro Doki, Masaki Mori, Kazuhiko Ogawa, Hideshi Ishii
CELL DEATH DISCOVERY
(2018)
Article
Physiology
Keiichiro Matoba, Daiji Kawanami, Masami Tsukamoto, Jun Kinoshita, Tomoko Ito, Sho Ishizawa, Yasushi Kanazawa, Tamotsu Yokota, Noriyuki Murai, Senya Matsufuji, Kazunori Utsunomiya
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2014)
Meeting Abstract
Endocrinology & Metabolism
Keiichiro Matoba, Daiji Kawanami, Rina Okada, Masami Tsukamoto, Jun Kinoshita, Tomoko Ito, Sho Ishizawa, Yasushi Kanazawa, Tamotsu Yokota, Noriyuki Murai, Senya Matsufuji, Junko Takahashi-Fujigasaki, Kazunori Utsunomiya
Meeting Abstract
Biochemistry & Molecular Biology
Yasuko Murakami, Junichiro Suzuki, Keijiro Samejima, Noriyuki Murai, Senya Matsufuji, Takami Oka
Article
Biochemistry & Molecular Biology
T Shimamura, A Koike-Takeshita, K Yokoyama, R Masui, N Murai, M Yoshida, H Taguchi, S Iwata
Article
Biochemistry & Molecular Biology
N Murai, Y Murakami, S Matsufuji
JOURNAL OF BIOLOGICAL CHEMISTRY
(2003)