Article
Immunology
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Summary: This study aimed to identify and compare the general differences in protein profiles among clinical strains of S. aureus sensitive and resistant to methicillin. The proteomic analysis revealed differences in protein abundances related to virulence, metabolism, and protein synthesis between MSSA and MRSA strains.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Junyuan Qin, Lei Yu, Fu Peng, Xin Ye, Gangmin Li, Chen Sun, Fang Cheng, Cheng Peng, Xiaofang Xie
Summary: In this study, tannins isolated from Penthorum chinense Pursh (TPCP) were found to exhibit antimicrobial and antibiofilm properties against Staphylococcus aureus. The study showed that TPCP effectively destroyed the bacterial biofilms formed by MRSA, inhibiting its growth and reducing exopolysaccharide and extracellular DNA secretion.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Francesca Sivori, Ilaria Cavallo, Daniela Kovacs, Maria Guembe, Isabella Sperduti, Mauro Truglio, Martina Pasqua, Grazia Prignano, Arianna Mastrofrancesco, Luigi Toma, Fulvia Pimpinelli, Aldo Morrone, Fabrizio Ensoli, Enea Gino Di Domenico
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) has become the leading cause of skin and soft tissue infections (SSTIs) and biofilm formation contributes to increased antibiotic tolerance. This study demonstrates that dalbavancin is the most effective antibiotic against MRSA biofilms and extracellular DNA affects its activity. The findings provide new insights for developing targeted therapeutic strategies against MRSA.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Jian Peng, Biswajit Mishra, Rajamohammed Khader, LewisOscar Felix, Eleftherios Mylonakis
Summary: In this study, a smaller antimicrobial peptide C18 derived from cecropin 4 showed high antibacterial activity against various bacteria and yeasts, with a remarkable effect on drug-resistant Staphylococcus aureus. C18 also exhibited the capability to eliminate persister cells, reduce biofilm adherence, interact with DNA, and down-regulate virulence factor genes. Moreover, C18 showed promising results in a Galleria mellonella model, highlighting its potential as a therapeutic candidate for severe MRSA infections.
Article
Infectious Diseases
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Summary: This study compared the lipid profiles, cell wall thickness, and cell surface charge between antibiotic sensitive and resistant clinical strains of S. aureus. It found differences in membrane lipids that may impact resistance mechanisms related to cell membrane structure and fluidity, but no significant differences were observed in cell wall thickness and whole-cell surface charge. Further research on these differences may identify new drug targets against resistant strains.
INFECTIOUS DISEASES
(2022)
Article
Microbiology
Hui Zhao, Lu Liu, Lingshuang Yang, Qihui Gu, Ying Li, Jumei Zhang, Shi Wu, Moutong Chen, Xinqiang Xie, Qingping Wu
Summary: The study identified a strain FJKB0103 isolated from the rhizosphere of Garcinia mangostana with strong anti-MRSA activity. Through genomic analysis, the researchers discovered that DAPG produced by the strain was the compound responsible for its activity. DAPG was found to be effective against MRSA strains and could inhibit biofilm formation.
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Sara Quero, Marina Serras-Pujol, Noemi Parraga-Nino, Carmen Torres, Marian Navarro, Anna Vilamala, Emma Puigoriol, Javier Diez de los Rios, Elisenda Arque, Judit Serra-Pladevall, Alba Romero, Daniel Molina, Roger Paredes, Maria Luisa Pedro-Botet, Esteban Reynaga
Summary: This study investigated the prevalence of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) among workers in the pork production chain. The results showed that the prevalence of MRSA was high among workers in contact with live animals, especially in pig farms, livestock transporters, and slaughterhouses.
Article
Pharmacology & Pharmacy
Jina Kim, Young-Won Chin
Summary: The prevalence of antimicrobial-resistant bacteria poses a global challenge. Methicillin-resistant Staphylococcus aureus (MRSA), a leading cause of infections, forms biofilms on medical devices, making them more resistant to antibiotics. A study investigated the use of Raman spectroscopy to monitor MRSA biofilm formation and found that Eugenol, when applied within the first 5 hours of biofilm formation, showed promise in breaking down the biofilm. The study also demonstrated the efficacy of Raman spectroscopy coupled with principal component analysis (PCA) in identifying planktonic bacteria from biofilm conglomerates.
Article
Food Science & Technology
Martina Florianova, Kristyna Korena, Helena Juricova
Summary: This study evaluated the prevalence of Staphylococcus aureus in dry-fermented salami and found multidrug-resistant and virulent strains. The source of contamination was identified as raw pork and human carriers. Therefore, maintaining high standards of hygiene and manufacturing practices in food processing is crucial.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Immunology
Syong H. Nam-Cha, Elena Dominguez-Jurado, Selena L. Tinoco-Valencia, Ramon Perez-Tanoira, Noelia Morata-Moreno, Rocio Alfaro-Ruiza, Agustin Lara-Sanchez, Jaime Esteban, Rafael Lujan, Carlos Alonso-Moreno, Pedro Segui, Alberto Ocana, Angel Lopez Gonzalez, John J. Aguilera-Correa, Francisco C. Perez-Martinez, Milagros Molina Alarcon
Summary: This study aimed to translate clinical research outcomes based on metallodrugs into clinical practice for the treatment of MRSA. The novel metallodrug 2P-Pt showed antibacterial effect against MRSA and inhibited biofilm formation. These findings suggest that 2P-Pt may be a promising therapeutic alternative for MRSA infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Pengfei She, Shijia Li, Linying Zhou, Yaqian Liu, Lanlan Xu, Zubair Hussain, Yimin Li, Zehao Li, Shasha Liu, Yong Wu
Summary: This study found that eltrombopag exhibits potential antimicrobial and anti-biofilm activities against Staphylococcus aureus, and interferes with the proton motive force in the bacteria. The in vivo anti-infective efficacy of the drug was confirmed in multiple infection models, with no observed cytotoxicity or in vivo toxicity. These findings suggest that eltrombopag could be a potential novel antimicrobial agent against MRSA infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Maria Simitsopoulou, Paschalis Kadiltzoglou, Charalampos Antachopoulos, Emmanuel Roilides
Summary: This study found that Daptomycin (DAP) has a synergistic effect on MRSA biofilms, enhancing the damage caused by human polymor-phonuclear neutrophils (PMNs). Additionally, DAP can modulate the immune response of human monocytes (MNCs) to MRSA biofilms through upregulation of Toll-like receptor 2 (TLR2) and increased NLRP3 inflammasome production, potentially controlling biofilm-associated pathogenicity.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Jelle Vlaeminck, Qiang Lin, Basil Britto Xavier, Sarah De Backer, Matilda Berkell, Henri De Greve, Jean-Pierre Hernalsteens, Samir Kumar-Singh, Herman Goossens, Surbhi Malhotra-Kumar
Summary: There are striking differences in gene expression during biofilm formation by three important pandemic MRSA clones, USA300, HEMRSA-15, and ST239. The clfA gene is the only significantly upregulated gene across all three strains in 24-hour biofilm cultures and represents an important target for disrupting early biofilms. Furthermore, our data indicate the critical role of arginine catabolism pathways in early biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marta Woroszylo, Daria Ciecholewska-Jusko, Adam Junka, Marcin Wardach, Grzegorz Chodaczek, Bartlomiej Dudek, Karol Fijalkowski
Summary: This study analyzed the impact of combining rotating magnetic field (RMF) with various classes of antibiotics on nine strains of Staphylococcus aureus. The results showed that combining RMF with antibiotics that interfere with cell walls, particularly beta-lactams, led to favorable changes in growth inhibition zones or minimal inhibitory concentration values, especially for methicillin-resistant strains. The study suggests that the application of magnetic fields could be an important strategy in combating infections caused by methicillin-resistant staphylococci due to the increased effectiveness of antibiotics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Keigo Yonemoto, Akio Chiba, Shinya Sugimoto, Chikara Sato, Mitsuru Saito, Yuki Kinjo, Keishi Marumo, Yoshimitsu Mizunoe
INFECTION AND IMMUNITY
(2019)
Article
Chemistry, Multidisciplinary
Tatsuki Kunoh, Kana Morinaga, Shinya Sugimoto, Shun Miyazaki, Masanori Toyofuku, Kenji Iwasaki, Nobuhiko Nomura, Andrew S. Utada
Article
Biochemistry & Molecular Biology
Shinya Sugimoto, Kunitoshi Yamanaka, Tatsuya Niwa, Yurika Terasawa, Yuki Kinjo, Yoshimitsu Mizunoe, Teru Ogura
Summary: This study demonstrated the role of JDPs in bacterial survival during heat stress and the formation of curli-extracellular amyloid fibers. Genetic analysis revealed the essential role of DnaJ in survival at high temperature and the ability of either DnaJ or CbpA to activate curli production. Biochemical analyses showed that DnaJ and CbpA are involved in regulating the master regulator CsgD and maintaining CsgA in a translocation-competent state.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Tatsuki Kunoh, Tatsuya Yamamoto, Shinya Sugimoto, Erika Ono, Nobuhiko Nomura, Andrew S. Utada
Summary: The study revealed that limitations in Na+, K+, and Fe2+ did not impact the growth of filamentous bacteria significantly, while limitations in C, N, P, or vitamins led to changes in filament morphology. Furthermore, the absence of Mg2+ and Ca2+ had dramatic effects on the cells, with Mg2+ loss resulting in cell autolysis and Ca2+ removal causing catastrophic disintegration of the filaments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Akio Chiba, Masahide Seki, Yutaka Suzuki, Yuki Kinjo, Yoshimitsu Mizunoe, Shinya Sugimoto
Summary: The study reveals the contribution of RNA to the structural integrity of Staphylococcus aureus biofilms. The primary source of RNA in the biofilm matrix was found to be the culture medium, and polysaccharides were critical for capturing and stabilizing external RNA, contributing to biofilm formation and organization.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Biotechnology & Applied Microbiology
Tatsuki Kunoh, Tatsuya Yamamoto, Manoj Prasad, Erika Ono, Xiaojie Li, Shinya Sugimoto, Eiji Iida, Nozomu Obana, Minoru Takeda, Nobuhiko Nomura, Andrew S. S. Utada
Summary: The bacterium Leptothrix cholodnii forms porous pellicles at the air-liquid interface by swimming towards the interface using its polar flagellum and intertwining with others with the assistance of its nanofibril sheath.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biology
Shinya Sugimoto, Yuki Kinjo
Summary: iCBiofilm is a new method for imaging whole bacterial and fungal biofilms using confocal laser scanning microscopy, which allows for the examination of the dynamics and functions of biofilms.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Tatsuki Kunoh, Tatsuya Yamamoto, Erika Ono, Shinya Sugimoto, Kyosuke Takabe, Minoru Takeda, Andrew S. Utada, Nobuhiko Nomura
Summary: The regulation of cell chain elongation of filamentous bacteria via extracellular signals has been studied to prevent clogging of water distribution systems and filamentous bulking of activated sludge in industrial settings. The bacterium Leptothrix cholodnii generates cell chains encased in sheaths composed of woven nanofibrils. This study identified a novel glycosyltransferase, LthB, involved in nanofibril biosynthesis and demonstrated the functional differences between LthA and LthB in cell chain elongation and pellicle formation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
S. Sugimoto, E. Kobayashi, M. Fujii, Y. Ohta, K. Ishikawa, K. Nanki, Y. Hakamata, T. Kanai, T. Sato
JOURNAL OF CROHNS & COLITIS
(2021)
Article
Biology
Shinya Sugimoto, Ken-ichi Arita-Morioka, Akari Terao, Kunitoshi Yamanaka, Teru Ogura, Yoshimitsu Mizunoe
COMMUNICATIONS BIOLOGY
(2018)
Meeting Abstract
Gastroenterology & Hepatology
Chikako Watanabe, Motohiro Esaki, Kenji Watanabe, Shiro Nakamura, Noriko Kamata, Hirokazu Yamagami, Kaoru Yokoyama, Naoki Yoshimura, Toshimitsu Fujii, Katsuyoshi Matsuoka, Masakazu Nagahori, Taku Kobayashi, Makoto Naganuma, Toshifumi Hibi, Ryota Hokari
Meeting Abstract
Gastroenterology & Hepatology
Shinichiro Shinzaki, Toshimitsu Fujii, Shigeki Bamba, Taku Kobayashi, Hiroki Tanaka, Takuya Yoshino, Akihiro Yamada, Noriko Kamata, Toshifumi Hibi
Meeting Abstract
Gastroenterology & Hepatology
Taku Kobayashi, Akihito Uda, Tomoka Mineyama, Eri Udagawa, Kosuke Iwasaki, Wentao Tang, Toshifumi Hibi
Meeting Abstract
Gastroenterology & Hepatology
M. Naganuma, S. Sugimoto, K. Mitsuyama, T. Kobayashi, N. Yoshimura, H. Ohi, K. Matsuoka, T. Hisamatsu, K. Watanabe, T. Abe, M. Watanabe, T. Hibi, Y. Suzuki, T. Kanai
JOURNAL OF CROHNS & COLITIS
(2018)
Meeting Abstract
Gastroenterology & Hepatology
T. Fukuda, M. Naganuma, S. Sugimoto, K. Ono, K. Nanki, S. Mizuno, K. Kimura, M. Mutaguchi, K. Takabayashi, N. Inoue, H. Ogata, Y. Iwao, T. Kanai
JOURNAL OF CROHNS & COLITIS
(2018)