期刊
RSC ADVANCES
卷 8, 期 41, 页码 22991-22997出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ra02596g
关键词
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资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [NRF-2016R1D1A1B03936000, 2017R1A4A1015564, 2017S1A5B6054769]
- National Research Foundation of Korea [2017R1A4A1015564, 2017S1A5B6054769] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This paper proposes a user-friendly and highly sensitive detection method for influenza A (H1N1) virus using the cooperation of quantum dot (Qdot)-aptamer beacons and light guide in a three-dimensional (3D) photonic crystal (PC). For easy use, we present a fluorescent probe-based OFF-ON' detection protocol. First, a mixture composed of Qdot-aptamer beacons and dark quencher-labeled guard DNA (G-DNA) was prepared. It initially quenched the fluorescent signals to significantly low intensity, i.e., OFF' state. Then, the influenza A (H1N1) virus preferentially bound to the aptamer and G-DNA was released, so that the fluorescent signal was restored and biosensor turned to ON' state. The restored fluorescence signal changed quantitatively according to the concentration of the target influenza A (H1N1) virus. Owing to the light guide of the 3D nanoporous PC structure, we achieved high sensitivity with ultra-low limit of detection (LOD) of 138 pg mL(-1) and high selectivity over other species of influenza A virus and biomolecules. Additionally, with the benefit of enhanced output fluorescent signals, the target virus could be easily detected with a low-cost and portable home-made setup (total cost of only 20 US dollars) and a built-in camera in a smartphone.
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