期刊
FOOD & FUNCTION
卷 9, 期 4, 页码 2300-2312出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8fo00206a
关键词
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资金
- Natural Science Foundation of Tianjin [16JCZDJC34100]
- National Spark Key Program of China [2015GA610001]
An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 degrees C) extraction and purified by Sephadex G-200. R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%. A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 x 10(5) Da. Monosaccharide composition analysis shows that it consists of D-arabinose, D-xylose, D-glucose, D-galactose, D-galacturonic acid, and D-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1: 186. Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of -> 1)-alpha-D-Glcp-(6 -> with side chains of -> 1)-beta-D-Galp-(4,6 ->, -> 1)-beta-D-Araf-(5 ->, -> 1)-alpha-D-Xylp-(4 ->, -> 1)-alpha-D-GlcpA-(2 -> and -> 1)-alpha-D-GalpA-(4 ->. Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis. The results show that R-PL does not have a triple-helical conformation in solution; its shape resembles twisted ribbons. Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.
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