Article
Materials Science, Multidisciplinary
Xiangyu Dong, Shuxiang Zhang, Yi Xu, Longquan Chen, Qiang Wei, Changsheng Zhao
Summary: A simple and smart method has been developed to glue living cells together and harvest their extracellular matrix (ECM). The obtained ECM coatings have fewer cell fragment residues compared to those obtained through traditional cell lysis. This method maintains the activity of ECM proteins, promoting cell adhesion and growth. Moreover, ECM coatings from different functional cells can guide stem cell differentiation.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2022)
Review
Engineering, Biomedical
Xingzhen Zhang, Sabine van Rijt
Summary: Stem cells have great potential in tissue engineering and regenerative medicine, but the challenge lies in predicting their behavior after transplantation; understanding the interplay between stem cells and their microenvironments through biointerfaces can help control stem cell behavior.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Viki Raz Lepsky, Sari Natan, Oren Tchaicheeyan, Avraham Kolel, Merav Zussman, Meital Zilberman, Ayelet Lesman
Summary: The study found that the addition of cells to fibrin gels slightly decreased drug release, with cellular activities affecting the release profile while forces generated by cells and matrix deformation had minimal impact on release profiles.
Article
Biochemistry & Molecular Biology
Vijaya Sarangthem, Harshita Sharma, Ridhima Goel, Sampa Ghose, Rang-Woon Park, Sujata Mohanty, Tapan Kumar Chaudhur, Amit Kumar Dinda, Thoudam Debraj Singh
Summary: Extracellular matrix molecules are important for regulating cell behavior and tissue repair. Researchers proposed the use of human elastin derived materials to mimic biological functions. Elastin-like polypeptides (ELPs) containing ECM domains were genetically synthesized to regulate cell behavior and mimic natural matrices. Fusion ELP derivatives promoted cell attachment, migration, and tube formation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Shunji Yunoki, Hirosuke Hatayama, Yoshimi Ohyabu, Kazuto Kobayashi
Summary: This study extracted collagen from barramundi fish scales and demonstrated its suitability for use in collagen fibril matrices (CFM). The fish scale collagen had high thermal stability and supported the well-developed growth of cells in 3D culture. This is the first report of fish CFM used in conventional 3D cell culture.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Yushi Yoshimasa, Tomoka Takao, Satomi Katakura, Shoko Tomisato, Hirotaka Masuda, Mamoru Tanaka, Tetsuo Maruyama
Summary: The use of ECM-based scaffolds, such as the decellularized endometrial scaffold (DES), can enhance the regeneration of endometrial stroma in the uterus. However, additional intervention(s) are needed to induce the regeneration of luminal and glandular epithelia. The prevention of adhesions alone can also lead to endometrial stroma regeneration, but to a lesser degree compared to DES.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Multidisciplinary
Yasaman Esmaeili, Elham Bidram, Ashkan Bigham, Mehdi Atari, Reyhaneh Nasr Azadani, Mohamadreza Tavakoli, Saeideh Salehi, Marjan Mirhaj, Arefeh Basiri, Zahra Mirzavandi, Maryam Boshtam, Mohammad Rafienia, Anousheh Zargar Kharazi, Saeid Karbasi, Laleh Shariati, Ali Zarrabi
Summary: The combination of tissue engineering and gene therapy, known as gene-activated matrix (GAM), offers a promising approach for restoring damaged tissues and organs. However, creating GAMs with low cytotoxicity, high transfection efficiency, and long-term gene delivery efficiency remains challenging. This article provides a comprehensive overview of various tissue engineering approaches and their ongoing preclinical research, with a focus on the development, application, and future prospects of GAMs. The challenges and future research prospects in regenerative medicine are also discussed.
ALEXANDRIA ENGINEERING JOURNAL
(2023)
Review
Biotechnology & Applied Microbiology
Afarin Neishabouri, Alireza Soltani Khaboushan, Faezeh Daghigh, Abdol-Mohammad Kajbafzadeh, Masoumeh Majidi Zolbin
Summary: Reproduction of different tissues using scaffolds and materials is an important aspect of regenerative medicine. Decellularized extracellular matrix (dECM) has been used for the goal of regenerating whole organs. Recently, decellularization techniques have been widely utilized in producing suitable scaffolds for damaged organ regeneration. The advantages of decellularized ECM include preserved natural microenvironment features. Different decellularization methods have been developed for specific tissues to remove cells. Evaluation techniques have also been advanced to improve the efficacy of decellularization methods. Modification techniques enhance the characteristics of decellularized scaffolds for damaged tissue regeneration and other applications. However, challenges still exist in the field of decellularization methods and scaffold application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Mireille M. J. P. E. Sthijns, Clemens A. van Blitterswijk, Vanessa L. S. LaPointe
Summary: This review proposed a novel strategy to generate tissue-specific ECM by releasing metabolic molecules through a synthetic material, which stimulates cells to form their own ECM. The approach is based on the modulation of ECM properties via cellular metabolism, targeting different levels of metabolism to achieve desired effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Biomedical
Wenlan Xiao, Yang Yang, Chenyu Chu, Sheng-An Rung, Zhanqi Wang, Yi Man, Jie Lin, Yili Qu
Summary: Biomaterials are effective treatment options for tissue defects in regenerative medicine. ECM scaffold materials composed of biopolymers can induce a pro-regenerative immune microenvironment and facilitate wound healing. Macrophages play a crucial role in the immune response to implanted biomaterials, and their polarization status reflects the characteristics of the immune microenvironment. High-resolution techniques have been used to identify distinct macrophage phenotypes involved in host responses to biomaterials.
BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ju An Park, Yejin Youm, Hwa-Rim Lee, Yongwoo Lee, Sarah L. Barron, Taejeong Kwak, Gyu Tae Park, Young-Cheol Song, Roisin M. Owens, Jae Ho Kim, Sungjune Jung
Summary: A cell sheet transfer method from a culture surface to a target tissue is introduced in this study. UV-treated parylene, a commercially available flexible material, is used as the culture surface to provide adequate cell adhesiveness for both cell attachment during culture and efficient transfer. Various cell types are successfully transferred using this versatile cell-transfer process. The mechanism of interfacial cell migration between surfaces with different adhesion preferences is observed, and the re-establishment and reversal of cell polarity after transfer are identified. The unique capabilities of 3D multilayer stacking, freeform design, and curved surface application are demonstrated. The therapeutic potential of the cell-sheet delivery system is demonstrated through experiments on cutaneous wound healing and skin-tissue regeneration in mice models.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Charles M. Bowen, Frederick S. Ditmars, Ashim Gupta, Jo-Anna Reems, William Samuel Fagg
Summary: Amniotic fluid, specifically its cell-free form, has regenerative properties and can stimulate immunomodulatory and pro-growth characteristics in target cells or tissues. It has been shown to effectively treat cutaneous wounds and orthopedic degenerative conditions.
Review
Engineering, Biomedical
Dimitrios Tsiapalis, Dimitrios I. Zeugolis
Summary: This article discusses the impact of macromolecular crowding on biochemical reactions, processes, and functions both intracellularly and extracellularly, highlighting the early stage of appreciating and effectively implementing its potential in cell culture systems.
Review
Engineering, Biomedical
Fengqing Shang, Yang Yu, Shiyu Liu, Leiguo Ming, Yongjie Zhang, Zhifei Zhou, Jiayu Zhao, Yan Jin
Summary: The utilization of regenerative biomaterials for bone repair requires not only efficacy and safety, but also cost-effectiveness and convenience. Understanding the roles of regenerative biomaterials and mesenchymal stem cells in new bone formation can potentially open new opportunities in the field of bone regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Article
Multidisciplinary Sciences
Baeckkyoung Sung, Alexis de la Cotte, Eric Grelet
NATURE COMMUNICATIONS
(2018)
Article
Engineering, Biomedical
Baeckkyoung Sung, Jess Krieger, Bing Yu, Min-Ho Kim
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2018)
Article
Chemistry, Multidisciplinary
Chang-Beom Park, Jae-Woong Jung, Minjeong Baek, Baeckkyoung Sung, Jin-Woo Park, Yohan Seol, Dong-Hyuk Yeom, June-Woo Park, Young Jun Kim
JOURNAL OF NANOPARTICLE RESEARCH
(2019)
Article
Multidisciplinary Sciences
Marie-Leonie Bohlen, Hyun Pyo Jeon, Young Jun Kim, Baeckkyoung Sung
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Biochemistry & Molecular Biology
Chang Gyun Park, Baeckkyoung Sung, Chang Seon Ryu, Young Jun Kim
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2020)
Article
Materials Science, Multidisciplinary
Min Jeong Baek, Jino Son, Jayoung Park, Yohan Seol, Baeckkyoung Sung, Young Jun Kim
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2020)
Article
Mathematics, Applied
Baeckkyoung Sung
MATHEMATICAL METHODS IN THE APPLIED SCIENCES
(2020)
Review
Biotechnology & Applied Microbiology
Baeckkyoung Sung, Min-Ho Kim, Leon Abelmann
Summary: Soft micro- and nanostructures, particularly magnetic microgels and nanogels, have been extensively developed for biomedical applications. These materials combine the advantages of hydrogels and colloidal particles, and offer a wide range of potential applications in biomedicine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Physics, Multidisciplinary
Baeckkyoung Sung, Huan Yan, Chanjoong Kim, Leon Abelmann
Article
Materials Science, Biomaterials
Baeckkyoung Sung, Deok-Ho Kim, Min-Ho Kim, Daniele Vigolo
Summary: The crawling behavior of leukocytes is driven by cell morphology transition, which is a direct manifestation of molecular motor machinery. This study focuses on understanding the combined effects of surface topography and elasticity on the shape and motility of neutrophils. The findings show that pattern anisotropy and elastic modulus of the substrate have synergistic effects on the shape, deformability, and polarization/alignment of crawling neutrophils. These results suggest that controlling immune cell functions can be achieved through predefined physical microenvironments.
Article
Biology
Baeckkyoung Sung
Summary: The organ-on-a-chip is an artificial microphysiological system that mimics dynamic and physiologically relevant features of the body. It is widely used to simulate complex hormone dynamics and endocrine signaling pathways. Mathematical and computational modeling approaches are crucial for analyzing and predicting the chip's responses.
MATHEMATICAL BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Baeckkyoung Sung, Leon Abelmann
Summary: This dataset demonstrates the agglomerate dimension and structure of oleic acid-coated superparamagnetic nanoparticles in a thermotropic liquid crystal, as well as the statistical information on the sizes and inter-particle spacing of the agglomerated nanoparticles. It supports the fundamental understanding of how colloidal nanospheres behave in an anisotropic fluid.
Article
Chemistry, Physical
Baeckkyoung Sung, Henricus Herman Wensink, Eric Grelet
Article
Radiology, Nuclear Medicine & Medical Imaging
Baeckkyoung Sung, Minsoo Kim, Min Su Kim, Jin-Kyu Lee, Kwang-Sup Soh
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS
(2017)