Review
Cell Biology
Katerina Turner, Nisha Solanki, Hassan O. Salouha, Tomer Avidor-Reiss
Summary: The sperm competition theory predicts that sperm evolve through a cascade of changes, including morphological diversification. A study on fish species found that internally fertilizing fish are more likely to evolve sperm with atypical centriolar composition compared to externally fertilizing fish. This finding supports the sperm competition theory.
Article
Developmental Biology
Farners Amargant, Aida Pujol, Anna Ferrer-Vaquer, Merce Durban, Meritxell Martinez, Rita Vassena, Isabelle Vernos
Summary: Through proteomics and immunofluorescence studies, it was discovered that the human zygote inherits a basal body enriched with centrosomal proteins from the sperm, establishing the first functional centrosome of the new organism. The centrosome contributes to the robustness of early cell divisions, increasing the probability of parthenotes reaching the compaction stage. In the absence of the sperm-derived centrosome, pericentriolar material components stored in the oocyte can form new structures de novo, suggesting tight control of PCM expression in zygotes.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Cell Biology
Ankit Jaiswal, Tatiana Baliu-Souza, Katerina Turner, Nagalakshmi Nadiminty, Amarnath Rambhatla, Ashok Agarwal, Stephen A. Krawetz, James M. Dupree, Barbara Saltzman, Samantha B. Schon, Tomer Avidor-Reiss
Summary: The study developed a FRAC assay to determine sperm centriole quality and found that it can identify men from couples with unexplained infertility as the likely source of infertility.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Mirjam I. Binner, Anna Kogan, Karin Panser, Alexander Schleiffer, Victoria E. Deneke, Andrea Pauli
Summary: Fertilization is a crucial process in sexual reproduction, but the molecular mechanisms behind it are still unclear. A study on zebrafish reveals that Spaca6, a conserved protein, is essential for sperm-egg binding and fusion. The research also uncovers a previously unknown dependence of Dcst2 expression on Spaca6 in sperm.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yoon Hee Jung, Hsiao-Lin Wang, Daniel Ruiz, Brianna J. Bixler, Hannah Linsenbaum, Jian-Feng Xiang, Samantha Forestier, Andrew M. Shafik, Peng Jin, Victor G. Corces
Summary: This study demonstrates that exposure of pregnant mice to bisphenol A (BPA) can lead to transgenerational transmission of obesity in F2 progeny up to the F6 generation. Chromatin accessibility analysis reveals alterations near binding motifs for CCCTC-binding factor (CTCF) at two cis-regulatory elements of the Fto gene, which correlate with the transmission of obesity. The interaction between these cis-regulatory elements and the Irx3 and Irx5 genes, involved in the differentiation of appetite-controlling neurons, is increased in sperm of obese mice. Deletion of the CTCF site in Fto results in mice that have normal food intake and fail to become obese when ancestrally exposed to BPA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Obstetrics & Gynecology
Sandhya Kumari, Sujith Raj Salian, Arpitha Rao, Shilpa M. Somagond, Ravindra R. Kamble, Aravind Nesaragi, Jyotirekha Das, G. K. Rajanikant, Srinivas Mutalik, Shamprasad Varija Raghu, Satish Kumar Adiga, Guruprasad Kalthur
Summary: This study aimed to explore the effects of novel quinoline derivatives on human sperm motility and functional competence. Among the nine derivatives tested, 6MQT and 2,6DQT showed significant enhancement in sperm motility and survival, with higher levels of capacitation, cAMP, and tyrosine phosphorylated proteins. In vitro fertilization experiments with Swiss albino mice showed that 6MQT resulted in higher blastocyst rate and quality, while 2,6DQT led to lower blastocyst rate compared to control.
REPRODUCTIVE SCIENCES
(2021)
Article
Cell Biology
Maria Milagros Giaccagli, Matias Daniel Gomez-Elias, Jael Dafne Herzfeld, Clara Isabel Marin-Briggiler, Patricia Sara Cuasnicu, Debora Juana Cohen, Vanina Gabriela Da Ros
Summary: The study investigated the role of mitochondrial activity in sperm fertilizing ability during capacitation in mice, finding that MMP increase depends on capacitation and H89-sensitive events, high MMP is associated with sperm motility, and mitochondrial function is needed mainly for zona pellucida penetration. Additionally, mitochondrial activity is fundamental for sperm function in fertilization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shaogeng Tang, Yonggang Luc, Will M. Skinnere, Mrinmoy Sanyala, Polina V. Lishko, Masahito Ikawa, Peter S. Kim
Summary: A study finds that knockout of Tmem95 gene causes male sterility in mice. Using a model experiment, it is discovered that human TMEM95 can bind to hamster egg membranes, and a potential receptor-binding surface region is revealed. Further experiments show that the specific, receptor-mediated interaction between sperm TMEM95 and eggs plays a crucial role in membrane fusion during fertilization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
L. Gonzalez-Brusi, J. G. Hamze, I Lamas-Toranzo, M. Jimenez-Movilla, P. Bermejo-Alvarez
Summary: This study aimed to identify sperm proteins interacting with the TMEM95 protein, which is essential for fertilization. Mass spectrometry identified 41 sperm proteins pulled down with TMEM95, suggesting an independent role of TMEM95 in fertilization. Among these proteins, OLFR601 is located in the acrosomal region and may mediate affinity for an odorant involved in fertilization. However, disruption of OLFR601 did not impair sperm fertilization ability, suggesting redundant function with other sperm proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Yena Jung, Hyewon Bang, Young-Hyun Kim, Na-Eun Park, Young-Ho Park, Chaeli Park, Sang-Rae Lee, Jeong-Woong Lee, Bong-Seok Song, Ji-Su Kim, Bo-Woong Sim, Dong-Won Seol, Gabbine Wee, Sunhyung Kim, Sun-Uk Kim, Ekyune Kim
Summary: The study investigated the functional role of VSIG1 in spermatogenesis and fertilization by knocking out VSIG1 in mouse embryos using CRISPR/Cas9. Results showed that the lack of VSIG1 did not impact spermatogenesis and male fertility in mice, suggesting it may not play essential roles in these processes. Further investigations are needed to understand the importance and molecular mechanism of VSIG1 in tissues of other species.
Article
Cell Biology
Juan J. Ferreira, Pascale Lybaert, Lis C. Puga-Molina, Celia M. Santi
Summary: Our study shows that CatSper channels in mouse and human sperm are activated through alkaline conditions and the presence of HCO3-, leading to an influx of Ca2+. Furthermore, HCO3- activates the soluble adenylyl cyclase/protein kinase A pathway, which sensitizes CatSper channels to respond to membrane depolarization, allowing for Ca2+ influx.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Joao C. Ribeiro, Raquel L. L. Bernardino, Ana Goncalves, Alberto Barros, Giuseppe Calamita, Marco G. G. Alves, Pedro F. F. Oliveira
Summary: Osmoregulation is essential for sperm function, and aquaglyceroporins play a crucial role in glycerol permeability, which is important for sperm motility. AQP7 is especially significant in glycerol transport, and its impairment may lead to reduced sperm motility in asthenozoospermic individuals.
Article
Cell Biology
Lin Chen, Junli Song, Jinglei Zhang, Zicong Luo, Xuren Chen, Canquan Zhou, Xiaoting Shen
Summary: This study found that SPACA4 is a protein expressed during sperm development, and its localization changes intracellularly, suggesting its potential role in human fertilization. However, there is no significant association between SPACA4 protein levels and fertilization or cleavage rates. Larger clinical trials are needed to evaluate the potential use of sperm SPACA4 protein levels in predicting fertilization potential.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Steven Serafini, Cristian O'Flaherty
Summary: Spermatozoa undergo capacitation, a process involving biochemical and morphological changes to enable fertilization. Reactive oxygen species (ROS) play a dual role in capacitation, regulating cellular processes at physiological levels but causing damage at high concentrations. Understanding redox signaling and phosphorylation events in sperm is crucial for fertility and developing treatments for male infertility.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Cell Biology
Gurman Grewal, Bahar Patlar, Alberto Civetta
Summary: Through RNA interference, it was found that knockdown of genes such as bor and CSN5 in Drosophila resulted in complete sterility, while knockdown of Manf led to a decrease in mature sperm production. Normal germ-line expression of Mst89B and CG31287 is essential for effective sperm usage and egg fertilization.
Review
Genetics & Heredity
Swati Pandruvada, Rachel Royfman, Tariq A. Shah, Puneet Sindhwani, James M. Dupree, Samantha Schon, Tomer Avidor-Reiss
Summary: Semen analysis is essential for evaluating male infertility but not sufficient for diagnosis. About 20% of infertile males have undetermined infertility. Limited treatment options exist due to lack of understanding of sperm defects frequency and reliable diagnostic tools.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Cell Biology
Alejandra Vasquez-Limeta, Kimberly Lukasik, Dong Kong, Catherine Sullenberger, Delgermaa Luvsanjav, Natalie Sahabandu, Raj Chari, Jadranka Loncarek
Summary: Centrioles assembly is regulated by CPAP during the cell cycle, and insufficiency of CPAP can lead to incomplete microtubule triplets and fragmentation of centrioles. These findings improve our understanding of the role of CPAP in centrosome biogenesis and its association with human diseases.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Cell Biology
Katerina Turner, Nisha Solanki, Hassan O. Salouha, Tomer Avidor-Reiss
Summary: The sperm competition theory predicts that sperm evolve through a cascade of changes, including morphological diversification. A study on fish species found that internally fertilizing fish are more likely to evolve sperm with atypical centriolar composition compared to externally fertilizing fish. This finding supports the sperm competition theory.
Article
Cell Biology
Tomer Avidor-Reiss, Luke Achinger, Rustem Uzbekov
Summary: Centrioles are subcellular organelles that are crucial for normal cell function and development. They are found in most animal cell types but are absent in egg cells and exhibit diverse characteristics in sperm cells. The precise role of centrioles in fertility and early embryo development remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Ankit Jaiswal, Tatiana Baliu-Souza, Katerina Turner, Nagalakshmi Nadiminty, Amarnath Rambhatla, Ashok Agarwal, Stephen A. Krawetz, James M. Dupree, Barbara Saltzman, Samantha B. Schon, Tomer Avidor-Reiss
Summary: The study developed a FRAC assay to determine sperm centriole quality and found that it can identify men from couples with unexplained infertility as the likely source of infertility.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Editorial Material
Cell Biology
Rustem E. E. Uzbekov, Tomer Avidor-Reiss
Editorial Material
Cell Biology
Heidi S. Fisher, Eduardo R. S. Roldan, Tomer Avidor-Reiss, Melissah Rowe
Review
Andrology
Aldo E. Calogero, Rossella Cannarella, Ashok Agarwal, Taha Abo-Almagd Abdel-Meguid Hamoda, Amarnath Rambhatla, Ramadan Saleh, Florence Boitrelle, Imad Ziouziou, Tuncay Toprak, Murat Gul, Tomer Avidor-Reiss, Parviz Kavoussi, Eric Chung, Ponco Birowo, Ramy Abou Ghayda, Edmund Ko, Giovanni Colpi, Fotios Dimitriadis, Giorgio Ivan Russo, Marlon Martinez, Gokhan Calik, Hussein Kandil, Gianmaria Salvio, Taymour Mostafa, Haocheng Lin, Hyun Jun Park, Nazim Gherabi, Nguyen Ho Vinh Phuoc, Nguyen Quang, Ricky Adriansjah, Sandro La Vignera, Sava Micic, Damayanthi Durairajanayagam, Ege Can Serefoglu, Vilvapathy Senguttuvan Karthikeyan, Priyank Kothari, Widi Atmoko, Rupin Shah
Summary: Infertility affects nearly 186 million people worldwide, with male infertility accounting for about half of the cases. There has been a decline in sperm concentration and total count over the past four decades, emphasizing the need for further research in Andrology.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Cell Biology
Rustem Uzbekov, Galina N. Singina, Ekaterina N. Shedova, Charles Banliat, Tomer Avidor-Reiss, Svetlana Uzbekova
Summary: Centrosome formation in mammals is derived from spermatozoa, except in certain animals like cattle, where the proximal centriole disappears by the end of the first cleavage division. The mechanisms of centriole formation are universal among different mammals, differing only in the stage of development.
Review
Genetics & Heredity
Tomer Avidor-Reiss, Rustem Uzbekov
Summary: It has been found that human spermatozoon may provide a second atypical centriole to the zygote, challenging the previous assumption that only one centriole is provided. Detailed ultrastructural analysis of bovine early embryos has identified the existence of atypical centrioles, raising doubts about the single-centriole model. Therefore, a reassessment of the role of paternal centrioles in human development is necessary.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Multidisciplinary Sciences
Katerina A. Turner, Luke Achinger, Dong Kong, Derek F. Kluczynski, Emily Lillian Fishman, Audrey Phillips, Barbara Saltzman, Jadranka Loncarek, Bo R. Harstine, Tomer Avidor-Reiss
Summary: This study found that evaluating sperm centriole quality can identify subfertility in cattle. The current methods are unable to detect subfertility in bulls, but fluorescence-based Ratiometric Analysis of Sperm Centrioles (FRAC) and acetylated tubulin labeling can provide insight into this novel mechanism of subfertility.
SCIENTIFIC REPORTS
(2023)
Review
Andrology
Ramy Abou Ghayda, Rossella Cannarella, Aldo E. Calogero, Rupin Shah, Amarnath Rambhatla, Wael Zohdy, Parviz Kavoussi, Tomer Avidor-Reiss, Florence Boitrelle, Taymour Mostafa, Ramadan Saleh, Tuncay Toprak, Ponco Birowo, Gianmaria Salvio, Gokhan Calik, Shinnosuke Kuroda, Raneen Sawaid Kaiyal, Imad Ziouziou, Andrea Crafa, Nguyen Ho Vinh Phuoc, Giorgio I. Russo, Damayanthi Durairajanayagam, Manaf Al-Hashimi, Taha Abo-Almagd Abdel-Meguid Hamoda, Germar-Michael Pinggera, Ricky Adriansjah, Israel Maldonado Rosas, Mohamed Arafa, Eric Chung, Widi Atmoko, Lucia Rocco, Haocheng Lin, Eric Huyghe, Priyank Kothari, Jesus Fernando Solorzano Vazquez, Fotios Dimitriadis, Nicolas Garrido, Sheryl Homa, Marco Falcone, Marjan Sabbaghian, Hussein Kandil, Edmund Ko, Marlon Martinez, Quang Nguyen, Ahmed M. Harraz, Ege Can Serefoglu, Vilvapathy Senguttuvan Karthikeyan, Dung Mai BaTien, Sunil Jindal, Sava Micic, Marina Bellavia, Hamed Alali, Nazim Gherabi, Sheena Lewis, Hyun Jun Park, Mara Simopoulou, Hassan Sallam, Liliana Ramirez, Giovanni Colpi, Ashok Agarwal
Summary: Artificial intelligence (AI) has made significant progress in the field of medicine and has been applied to various areas. Advances in computer science, medical informatics, robotics, and personalized medicine have facilitated AI's role in modern healthcare. In andrology and reproductive medicine, AI applications such as machine learning, artificial neural networks, and deep learning have shown great potential in diagnosing and treating male infertility, as well as improving patient care.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Andrology
Ala'a Farkouh, Ashok Agarwal, Taha Abo-Almagd Abdel-Meguid Hamoda, Parviz Kavoussi, Ramadan Saleh, Armand Zini, Mohamed Arafa, Ahmed M. Harraz, Murat Gul, Vilvapathy Senguttuvan Karthikeyan, Damayanthi Durairajanayagam, Amarnath Rambhatla, Florence Boitrelle, Eric Chung, Ponco Birowo, Tuncay Toprak, Ramy Abou Ghayda, Rossella Cannarella, Nguyen Ho Vinh Phuoc, Fotios Dimitriadis, Giorgio Russo, Ioannis Sokolakis, Taymour Mostafa, Konstantinos Makarounis, Imad Ziouziou, Shinnosuke Kuroda, Marion Bendayan, Raneen Sawaid Kaiyal, Andrian Japari, Mara Simopoulou, Lucia Rocco, Nicolas Garrido, Nazim Gherabi, Kadir Bocu, Oguzhan Kahraman, Tan Le, Christine Wyns, Kelton Tremellen, Selcuk Sarikaya, Sheena Lewis, Donald P. Evenson, Edmund Ko, Aldo E. Calogero, Fahmi Bahar, Marlon Martinez, Andrea Crafa, Quang Nguyen, Rafael F. Ambar, Giovanni Colpi, Mustafa Emre Bakircioglu, Ralf Henkel, Hussein Kandil, Ege Can Serefoglu, Abdullah Alarbid, Akira Tsujimura, Alireza Kheradmand, Christina Anagnostopoulou, Angelo Marino, Aram Adamyan, Birute Zilaitiene, Cevahir Ozer, Edoardo Pescatori, Paraskevi Vogiatzi, Gian Maria Busetto, Giancarlo Balercia, Haitham Elbardisi, Hamed Akhavizadegan, Hesamoddin Sajadi, Hisanori Taniguchi, Hyun Jun Park, Israel Maldonado Rosas, Mohamed Al-Marhoon, Mohammad Ali Sadighi Gilani, Naif Alhathal, Germar-Michael Pinggera, Priyank Kothari, Nasser Mogharabian, Sava Micic, Sheryl Homa, Sara Darbandi, Tran Quang Tien Long, Wael Zohdy, Widi Atmoko, Marjan Sabbaghian, Wael Ibrahim, Ryan P. Smith, Christopher Chee Kong Ho, Jean de la Rosette, Ahmed I. El-Sakka, Mirko Preto, Catalina Zenoaga-Barbarosie, Saad Mohammed Abumelha, Aykut Baser, Kaan Aydos, Liliana Ramirez-Dominguez, Vijay Kumar, Teng Aik Ong, Tiago Cesar Mierzwa, Ricky Adriansjah, Saleem A. Banihani, Kasonde Bowa, Shinichiro Fukuhara, Marcelo Rodriguez Pena, Mohamad Moussa, Umut Cagin Ari, Chak-Lam Cho, Nicholas N. Tadros, Muhammet Rasit Ugur, Edouard Amar, Marco Falcone, Frederic Romain Santer, Arif Kalkanli, Keshab Kumar Karna, Kareim Khalafalla, Ranjit B. Vishwakarma, Federica Finocchi, Carlo Giulioni, Erman Ceyhan, Gokhan Ceker, Chadi Yazbeck, Osvaldo Rajmil, Mehmet Yilmaz, Baris Altay, Trenton L. Barrett, Kay Seong Ngoo, Shubhadeep Roychoudhury, Gianmaria Salvio, Haocheng Lin, Ates Kadioglu, Massimiliano Timpano, Tomer Avidor-Reiss, Lukman Hakim, Puneet Sindhwani, Giorgio Franco, Rajender Singh, Filippo Giacone, Mikhail Ruzaev, Raghavender Kosgi, Nikolaos Sofikitis, Ayad Palani, Gokhan Calik, Deniz Kulaksiz, Davor Jezek, Manaf Al Hashmi, Panagiotis Drakopoulos, Huda Omran, Sofia Leonardi, Ciler Celik-Ozenci, Nur Dokuzeylul Gungor, Jonathan Ramsay, Toshiyasu Amano, Emrullah Sogutdelen, Gede Wirya Kusuma Duarsa, Koji Chiba, Sunil Jindal, Missy Savira, Luca Boeri, Edson Borges, Deepak Gupte, Fatih Gokalp, Guadalupe Hernandez Hebrard, Suks Minhas, Rupin Shah
Summary: The purpose of this study was to investigate global practices related to the management of elevated SDF in infertile men, summarize the relevant professional society recommendations, and provide expert recommendations for managing this condition. The results of a large global survey on the management of infertile men with elevated SDF revealed a lack of consensus among clinicians and highlighted the scarcity of professional society guidelines in this regard.
WORLD JOURNAL OF MENS HEALTH
(2023)
Meeting Abstract
Obstetrics & Gynecology
T. Avidor-Reiss
HUMAN REPRODUCTION
(2022)
