Article
Biochemistry & Molecular Biology
Linke Tang, Weifeng Yuan, Shitao Li, Xiuyan Ding, Liqian Zhu
Summary: In this study, the interaction between UV-primed global DDR and BoHV-1 productive infection was demonstrated. UV-primed global DDR differentially modulated the transcription of virus genes and stabilization of virus protein. Vice versa, the virus infection may affect UV-primed DDR signaling.
Article
Microbiology
Weiyin Xu, Ping Yan, Ziyan Zhou, Jingting Yao, Haochun Pan, Luyao Jiang, Zongyi Bo, Bo Ni, Mingxia Sun, Song Gao, Changchao Huan
Summary: Pseudorabies virus (PRV) infection in swine can cause high morbidity and mortality, leading to significant economic losses. In this study, the role of histone deacetylase 6 (HDAC6) in PRV infection was investigated. The inhibition of HDAC6 significantly decreased PRV replication, while its overexpression promoted PRV replication. PRV infection induced DNA damage response (DDR), and HDAC6 inhibition and knockout decreased DDR, suggesting that HDAC6 may be a crucial factor in promoting PRV replication.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Jiangbin Wei, Qiwu Shi, Lidan Xiong, Guang Xin, Tao Yi, Yunqing Xiao, Wanxia Huang
Summary: The study investigated the biological effects of radiation from the SG-III prototype laser facility on HaCat cells and PC12 cells, finding that the damage to cells was dose-dependent and induced DNA damage and apoptotic necrosis. Transcriptome and protein analysis revealed pathways involving proliferation, transformation, necrosis, inflammation response, apoptosis, and DNA damage repair were activated, suggesting potential harm to biological safety.
SCIENTIFIC REPORTS
(2021)
Review
Biology
Asako J. Nakamura
Summary: As a Japanese woman researcher, I have noticed gender bias in the field of science in Japan. Following the Fukushima nuclear Power Plant accident, I have sometimes felt that female researchers would be more suitable for communicating research results and specialized knowledge to the general public. The importance of STEAM education has been emphasized globally, and I believe that radiation science fits perfectly into this category. STEAM education is for all genders, and I hope that research in radiation science will inspire younger generations and contribute to an increase in gender balance among Japanese scientific researchers.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Elias Einig, Chao Jin, Valentina Andrioletti, Boris Macek, Nikita Popov
Summary: Deregulation of RNA Polymerase II (RNAPII) leads to collisions with DNA synthesis machinery, resulting in DNA damage and genomic instability in tumor cells. This study provides evidence that elongating RNAPII can activate the ATM kinase at the conflict regions to stimulate DNA repair. The study also reveals the involvement of WRNIP1 in limiting ATM activation during the normal cell cycle.
NATURE COMMUNICATIONS
(2023)
Article
Virology
Xin Ming, Huan Chen, Ying Yang, Pu Zhao, Liumei Sun, Caisheng Zhang, Hyun-Jin Shin, Jeong-Soo Lee, Yong-Sam Jung, Yingjuan Qian
Summary: This study reveals that the porcine epidemic diarrhea virus (PEDV) induces DNA damage response (DDR) through the ROS-ATM and caspase7-CAD-gamma H2AX signaling pathways, which facilitates its early replication.
Article
Biochemistry & Molecular Biology
Lingyu Qiu, Wenchao Xu, Xiaopeng Lu, Feng Chen, Yongcan Chen, Yuan Tian, Qian Zhu, Xiangyu Liu, Yongqing Wang, Xin-Hai Pei, Xingzhi Xu, Jun Zhang, Wei-Guo Zhu
Summary: HDAC6 regulates DNA damage signaling by controlling the mismatch repair and nucleotide excision repair pathways. It negatively regulates DNA double-strand break (DSB) repair in an enzyme activity-independent manner. HDAC6 interacts with H2A/H2A.X to prevent its interaction with the E3 ligase RNF168, and DSBs lead to the degradation of HDAC6 and the restoration of the interaction between RNF168 and H2A/H2A.X, facilitating the recruitment of DSB repair factors to chromatin and subsequent DNA repair.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
S. Schumann, H. Scherthan, K. Pfestroff, S. Schoof, A. Pfestroff, P. Hartrampf, N. Hasenauer, A. K. Buck, M. Luster, M. Port, M. Lassmann, U. Eberlein
Summary: This study provides novel data on ex vivo DSB repair in internally irradiated PBMCs of patients before radionuclide therapy, showing efficient repair occurs after internal irradiation with 50 mGy absorbed dose, and that the induction and repair rate after I-131 exposure is comparable to that of external irradiation with gamma- or X-rays.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Oncology
Xiaoyue Zhang, Peishan Liu, Xiang Zheng, Jia Wang, Qiu Peng, Zhengshuo Li, Lingyu Wei, Can Liu, Yangge Wu, Yuqing Wen, Qun Yan, Jian Ma
Summary: m6A modification plays a crucial role in regulating biological processes, including the DNA damage response. METTL3 and YTHDFs control ATM expression through m6A modification, affecting the DNA damage response.
Article
Plant Sciences
Xue Wang, Juping Wang, Huiting Cui, Weilong Yang, Bin Yu, Chi Zhang, Jiangqi Wen, Junmei Kang, Zhen Wang, Qingchuan Yang
Summary: The MtUGT84A1 gene plays important roles in anthocyanin accumulation and feedback inhibition of JA signaling in Medicago truncatula plants, which is crucial for plant protection under extreme light conditions.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Immunology
Sae Sakama, Keisuke Kurusu, Mayu Morita, Takashi Oizumi, Shinya Masugata, Shohei Oka, Shinya Yokomizo, Mayumi Nishimura, Takamitsu Morioka, Shizuko Kakinuma, Yoshiya Shimada, Asako J. Nakamura
Summary: The study assessed the effects of enriched environment on radiation-induced carcinogenesis using a mouse model. The results showed that enrichment before radiation exposure improved responsiveness to DNA damage and immunity, further suppressing chronic inflammatory response, which may reduce the risk of radiation-induced carcinogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Neetu Saini, Geetha Bheeshmachar, Apurva Sarin
Summary: Notch signaling pathway plays an important role in cell fate decisions and tissue homeostasis. This study reveals that the putative nucleolar localization sequence (NoLS) in the Notch1 intracellular domain (NIC1) regulates its localization in the nucleolus and anti-apoptotic activity, which is regulated by the deacetylase Sirtuin1. The study also shows that the lysine residues in NoLS are targets of Sirtuin1 activity, and the nucleolar intermediate proteins fibrillarin and nucleolin are required for the anti-apoptotic activity triggered by NIC1 localization to the nucleolus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yixiu Wang, Yuwei Xie, Bingzi Dong, Weijie Xue, Shuhai Chen, Shimada Mitsuo, Hao Zou, Yujie Feng, Kai Ma, Qian Dong, Jingyu Cao, Chengzhan Zhu
Summary: This study investigated the biological function and clinical significance of tweety homolog 3 (TTYH3) in hepatocellular carcinoma (HCC). It was found that TTYH3 overexpression promoted cell proliferation, migration, and invasion, inhibited cell apoptosis, and enhanced tumor formation and metastasis in vivo. TTYH3 regulated the MK5/GSK3-β-Catenin signaling pathway and promoted its own expression through a positive feedback loop in HCC.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Geriatrics & Gerontology
H. G. Keizer, R. Brands, W. Seinen
Summary: The purpose of this article is to investigate the role of the AMP-kinase pathway in the induction of health benefits by exercise, drugs, and health ingredients, and its relation to ageing. It is challenging to understand how the activation of a single biochemical pathway can produce various concurrent health benefits. The AMP-kinase pathway functions as an integrated stress response system with a feedback loop, detecting changes in ratios and toxins, and activating a protective response to promote longevity. Inactivation of the AMP-kinase pathway with age explains the negative impact on health benefits. The presence of a feedback loop positions this pathway as an AMPK-dependent integrated stress response system.
Article
Biochemistry & Molecular Biology
George Rasti, Maximilian Becker, Berta N. Vazquez, Maria Espinosa-Alcantud, Irene Fernandez-Duran, Andres Gamez-Garcia, Alessandro Ianni, Jessica Gonzalez, Laia Bosch-Presegue, Anna Marazuela-Duque, Anna Guitart-Solanes, Sandra Segura-Bayona, Joan-Josep Bech-Serra, Michael Scher, Lourdes Serrano, Uma Shankavaram, Hediye Erdjument-Bromage, Paul Tempst, Danny Reinberg, Mireia Olivella, Travis H. Stracker, Carolina de la Torre, Alejandro Vaquero
Summary: SIRT1 plays a crucial role in regulating the DNA damage response by interacting with the PP4 phosphatase complex to control the signaling of DNA damage and repair.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Yuping Chen, Jinhuan Wu, Guang Liang, Guohe Geng, Fei Zhao, Ping Yin, Somaira Nowsheen, Chengming Wu, Yunhui Li, Lei Li, Wootae Kim, Qin Zhou, Jinzhou Huang, Jiaqi Liu, Chao Zhang, Guijie Guo, Min Deng, Xinyi Tu, Xiumei Gao, Zhongmin Liu, Yihan Chen, Zhenkun Lou, Kuntian Luo, Jian Yuan
Article
Oncology
Jiaqi Liu, Guohe Geng, Guang Liang, Ling Wang, Kuntian Luo, Jian Yuan, Shiguang Zhao
ANNALS OF TRANSLATIONAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Qin Zhou, Jinzhou Huang, Chao Zhang, Fei Zhao, Wootae Kim, Xinyi Tu, Yong Zhang, Somaira Nowsheen, Qian Zhu, Min Deng, Yuping Chen, Bo Qin, Kuntian Luo, Baohua Liu, Zhenkun Lou, Robert W. Mutter, Jian Yuan
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Jinzhou Huang, Qin Zhou, Ming Gao, Somaira Nowsheen, Fei Zhao, Wootae Kim, Qian Zhu, Yusuke Kojima, Ping Yin, Yong Zhang, Guijie Guo, Xinyi Tu, Min Deng, Kuntian Luo, Bo Qin, Yuichi Machida, Zhenkun Lou
Review
Biochemistry & Molecular Biology
Yunhui Li, Jian Yuan
Summary: DNA is the hereditary material in humans and most other organisms, crucial for accurate transmission of genetic information. Damage to DNA, including DSBs, can lead to gene mutations, genome instability, and even tumorigenesis if repair mechanisms are defective. Protein deubiquitination is essential in DNA DSB repair, and understanding the molecular mechanisms of DUB regulation can provide insights for combatting human diseases and developing new therapeutic approaches.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2021)
Article
Genetics & Heredity
Wootae Kim, Fei Zhao, Huanyao Gao, Sisi Qin, Jing Hou, Min Deng, Jake A. Kloeber, Jinzhou Huang, Qin Zhou, Guijie Guo, Ming Gao, Xiangyu Zeng, Shouhai Zhu, Xinyi Tu, Zheming Wu, Yong Zhang, Ping Yin, Scott H. Kaufmann, Kuntian Luo, Zhenkun Lou
Summary: This study identifies the deubiquitinating enzyme USP13 as a key regulator of TopBP1, stabilizing it and coordinating the replication stress response. High expression of USP13 enhances cancer cell chemoresistance and correlates with poor prognosis in cancer patients.
Article
Cell Biology
Fei Zhao, Wootae Kim, Huanyao Gao, Chao Liu, Yong Zhang, Yuping Chen, Min Deng, Qin Zhou, Jinzhou Huang, Qi Hu, Shih-Hsun Chen, Somaira Nowsheen, Jake A. Kloeber, Bo Qin, Ping Yin, Xinyi Tu, Guijie Guo, Sisi Qin, Chao Zhang, Ming Gao, Kuntian Luo, Yilun Liu, Zhenkun Lou, Jian Yuan
Summary: ASTE1, as a downstream effector of the shieldin complex, cleaves single-stranded and 3' overhang DNA to promote shieldin-dependent non-homologous end-joining, leading to impaired DSB repair and resistance to PARP inhibitors in BRCA1-deficient cells.
NATURE CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Qian Zhu, Qiaoyan Yang, Xiaopeng Lu, Hui Wang, Lili Tong, Zheng Li, Ge Liu, Yantao Bao, Xingzhi Xu, Luo Gu, Jian Yuan, Xiangyu Liu, Wei-Guo Zhu
Summary: The study found that H3K14 trimethylation enhances ATR activation by recruiting the RPA complex to chromatin, thus facilitating the response to replication stress. Increased levels of H3K14me3 make cells more sensitive to replication stress, affecting cell cycle progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Ines Sturmlechner, Cheng Zhang, Chance C. Sine, Erik-Jan van Deursen, Karthik B. Jeganathan, Naomi Hamada, Jan Grasic, David Friedman, Jeremy T. Stutchman, Ismail Can, Masakazu Hamada, Do Young Lim, Jeong-Heon Lee, Tamas Ordog, Remi-Martin Laberge, Virginia Shapiro, Darren J. Baker, Hu Li, Jan M. van Deursen
Summary: The cell-cycle inhibitor p21 activates Rb-dependent transcription to generate a complex bioactive secretome, known as PASP, which includes chemokine CXCL14 that attracts macrophages. Macrophages disengage if cells normalize p21 within 4 days, but persisting p21 induction leads to M1 polarization and cytotoxic T cell response for eliminating target cells, including preneoplastic cells. Thus, p21 concurrently induces proliferative arrest and immunosurveillance of cells under duress.
Article
Multidisciplinary Sciences
Qian Zhu, Jinzhou Huang, Hongyang Huang, Huan Li, Peiqiang Yi, Jake A. Kloeber, Jian Yuan, Yuping Chen, Min Deng, Kuntian Luo, Ming Gao, Guijie Guo, Xinyi Tu, Ping Yin, Yong Zhang, Jun Su, Jiayi Chen, Zhenkun Lou
Summary: The study reveals that RNF19A promotes cancer cell sensitivity to PARPi by negatively regulating the BRCA1-BARD1 complex and inhibiting homologous recombination, thereby enhancing therapeutic efficiency in breast cancer.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Yang Su, Chenming Wu, Yiming Chang, Lei Li, Yuping Chen, Xuebing Jia, Xinshu Wang, Ying Lv, Bentong Yu, Jian Yuan
Summary: This study reveals the mechanism of USP17L2-SIRT7 axis in regulating chemoresistance of breast cancer cells, and also elucidates the role of USP17L2 as a potential therapeutic target and prognostic factor in breast cancer patients.
BREAST CANCER RESEARCH AND TREATMENT
(2022)
Article
Oncology
Xiaoxiang Sun, Huanyin Tang, Yu Chen, Zhixi Chen, Zhiyi Hu, Zhen Cui, Yaming Tao, Jian Yuan, Yun Fu, Zhigang Zhuang, Qizhi He, Qian Li, Xianghong Xu, Xiaoping Wan, Ying Jiang, Zhiyong Mao
Summary: Sun et al. have identified USP15 as a regulator of PARP1 stability in triple-negative breast cancer and demonstrated that ER, PR, and HER2 inhibit USP15-mediated PARP1 stabilization through various mechanisms. This finding is important for understanding the mechanism of PARP1 stability in triple-negative breast cancer.