Article
Biochemistry & Molecular Biology
Chow-Seng Kong, Alexandra Almansa Ordonez, Sarah Turner, Tina Tremaine, Joanne Muter, Emma S. Lucas, Emma Salisbury, Rita Vassena, Gustavo Tiscornia, Ali A. Fouladi-Nashta, Geraldine Hartshorne, Jan J. Brosens, Paul J. Brighton
Summary: The study found that uterine natural killer cells play a crucial role in embryo biosensing and implantation process, highlighting their importance in selectively eliminating senescent decidual cells.
Article
Cell Biology
Jing Long, Weike Li, Mengyue Chen, Yubin Ding, Xuemei Chen, Chao Tong, Na Li, Xueqing Liu, Junlin He, Chuan Peng, Yanqing Geng, Taihang Liu, Xinyi Mu, Fangfang Li, Yingxiong Wang, Rufei Gao
Summary: Uterine deficiency of Dnmt3b impairs decidualization and consequent embryo implantation defects. Pgk1 may play a significant role in the decidualization process.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Reproductive Biology
Susanne C. Grund, Xin Xin Wu, Dominik Mueller, Gunther Wennemuth, Ruth Gruemmer
Summary: Deletion of claudin-3 in the endometrium impairs decidualization and leads to reduced size of implantation sites and fetal weight. Transcription downregulation of various decidua-associated genes may contribute to the impairment of decidualization.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Wenhao Chen, Qian Zhang, Haibin Wang, Dongmei Tan, Yi Tan
Summary: The study found that GABAB receptors play an important role in embryo implantation and decidualization in mice, particularly with the significant upregulation of GABAB1 expression during specific periods. Hormones do not directly affect the expression of GABAB1, and agonists and antagonists of the whole GABAB receptors have weak effects on stromal cell proliferation and differentiation.
Article
Reproductive Biology
Ryan M. Brown, Linda Wang, Anqi Fu, Athilakshmi Kannan, Michael Mussar, Indrani C. Bagchi, Joan S. Jorgensen
Summary: Irx3 promotes successful embryo-uterine interactions in mice.
BIOLOGY OF REPRODUCTION
(2022)
Review
Reproductive Biology
Erika Elinor Paulson, Pierre Comizzoli
Summary: The review aims to summarize the current data on the mechanisms involved in uterine receptivity and embryo implantation in carnivores and identify research priorities to better understand a key phenomenon in a critical group of mammals.
BIOLOGY OF REPRODUCTION
(2021)
Review
Obstetrics & Gynecology
Awoniyi O. Awonuga, Olivia G. Camp, Husam M. Abu-Soud, Daniel A. Rappolee, Elizabeth E. Puscheck, Michael P. Diamond
Summary: The contributions of uterine endometrium and embryo in implantation success are still debated. Various measures, such as endometrial receptivity analysis (ERA), endometrial scratch (ES), endometrial microbiome, and intrauterine or intravenous measures, are recommended to improve implantation success. However, there is insufficient evidence to determine the relative importance of endometrium and embryo in implantation success and the utility of these measures, especially with euploid embryos. Evidence suggests that embryos can implant and develop on non-endometrium epithelium. The role of embryo euploidy in implantation success is currently underappreciated.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Pramod Dhakal, Harriet C. Fitzgerald, Andrew M. Kelleher, Hongyu Liu, Thomas E. Spencer
Summary: The study demonstrated the crucial roles of uterine glands in embryo survival and stromal cell decidualization. Using a glandless FOXA2-deficient mouse model, researchers found compromised embryo survival and decidualization on gestational day 6.5, leading to abrupt pregnancy loss by day 7.5.
Article
Toxicology
Dan Wang, Weike Li, Chengshun Yang, Xuemei Chen, Xueqing Liu, Junlin He, Chao Tong, Chuan Peng, Yubin Ding, Yanqing Geng, Xianqing Cao, Fangfang Li, Rufei Gao, Yingxiong Wang
Summary: The study revealed that ethylparaben and propylparaben significantly affect endometrial decidualization in pregnant mice, leading to compromised embryo implantation. Exposure to high doses of ethylparaben and propylparaben resulted in increased levels of progesterone and estrogen, decreased expression of uterine hormone receptors, and ultimately impacted pregnancy outcomes.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Obstetrics & Gynecology
Junmei Fan, Junkun Zhang, Suming Xu, Huiping Liu, Weigang Lv, Xingyu Bi, Yanling Liu, Wenjing Shi, Yuxia Zhang, Xueqing Wu
Summary: Uterine artery Doppler, particularly mPSV and mEDV during the implantation window, was useful for predicting clinical pregnancy, and AEDF was related to spontaneous abortion in the first trimester. Uterine artery Doppler combined with artificial abortion history, waist-to-hip ratio, LH of P administration day, mPSV and mEDV have a higher predictive value than uterine artery Doppler alone regarding the pregnancy from the first FET.
BMC PREGNANCY AND CHILDBIRTH
(2023)
Article
Multidisciplinary Sciences
Yiping Fan, Ryan Wai Kheong Lee, Xiang Wen Ng, Caroline E. Gargett, Jerry Kok Yen Chan
Summary: Improvements in reproductive techniques have increased live birth rates from IVF procedures, but the rate has plateaued recently. Endometrial receptivity deficiencies are identified as the main hindrance for successful implantation in patients with recurrent implantation failure (RIF). The impact of local endometrial injury (LEI) on perivascular endometrial mesenchymal stem/progenitor cells (eMSCs) is unknown, but it may play a role in improving IVF outcomes for RIF patients.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Diana Monsivais, Takashi Nagashima, Renata Prunskaite-Hyyrylainen, Kaori Nozawa, Keisuke Shimada, Suni Tang, Clark Hamor, Julio E. Agno, Fengju Chen, Ramya P. Masand, Steven L. Young, Chad J. Creighton, Francesco J. DeMayo, Masahito Ikawa, Se-Jin Lee, Martin M. Matzuk
Summary: The study reveals that bone morphogenetic proteins control endometrial receptivity in mice through the ACVR2A and SMAD1/5 signaling pathway, with ACVR2B being dispensable during embryo implantation.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Shuo Wan, Yadong Sun, Jinbao Zong, Wanqing Meng, Jiacong Yan, Kexin Chen, Sanfeng Wang, Daji Guo, Zhiqiang Xiao, Qinghua Zhou, Zhinan Yin, Meixiang Yang
Summary: Infertility is a global reproductive health problem, and the role of m(6)A modification in infertility is still unknown. This study reveals that METTL3-dependent m(6)A methylation plays a crucial role in female fertility by balancing estrogen and progesterone signaling.
CELL DEATH & DISEASE
(2023)
Article
Immunology
Rumiana Ganeva, Dimitar Parvanov, Nina Vidolova, Margarita Ruseva, Maria Handzhiyska, Katarina Arsov, Ivon Decheva, Dimitar Metodiev, Veselina Moskova-Doumanova, Georgi Stamenov
Summary: The objective of this study was to compare the quantities and ratios of immune cells in the endometrium during the mid-luteal phase between women with recurrent implantation failure (RIF) with successful and unsuccessful embryo implantation. Endometrial biopsies from 116 women undergoing Assisted Reproductive Technology (ART) were stained for different immune cells, and the quantities and ratios were compared based on the outcome of embryo implantation in the subsequent cycle. The results showed that certain ratios of immune cells were significantly different between the successful and unsuccessful implantation groups, and could be predictive factors for successful implantation in RIF patients.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Cell Biology
Eliza Grbac, Teresa So, Swati Varshney, Nicholas Williamson, Evdokia Dimitriadis, Ellen Menkhorst
Summary: Research suggests that prednisolone affects the expression of cytokines and protein production in human endometrial stromal fibroblasts (hESF), altering their regulation of trophoblast cells. Decidualization of hESF in the presence of prednisolone enhances trophoblast cell growth and influences the expression of cell motility genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Heather L. Chaney, Lindsay F. Grose, Gilles Charpigny, Susanta K. Behura, I. Martin Sheldon, James G. Cronin, Patrick Lonergan, Thomas E. Spencer, Daniel J. Mathew
Summary: This study identified biologically relevant IFNT-stimulated genes in specific endometrial cell types. The findings provide critical information on the effects of conceptus IFNT on specific endometrial compartments during early developmental processes in cattle.
BIOLOGY OF REPRODUCTION
(2021)
Article
Genetics & Heredity
Ronald J. Nowling, Susanta K. Behura, Marc S. Halfon, Scott J. Emrich, Molly Duman-Scheel
Summary: The Aedes aegypti genome has been re-sequenced and re-assembled with improved quality. The updated genome assembly has led to the reprocessing of historical functional genomic data sets, resulting in a validated CRE map. A new software called PeakMatcher has been developed for matching peaks across genome assemblies, facilitating the validation and transferring of previous annotations.
Article
Biochemistry & Molecular Biology
Pramod Dhakal, Thomas E. Spencer
Summary: Prss28 and Prss29 are not essential for female fertility and do not affect endometrial gland development or uterine function in mice.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Chelsie B. Steinhauser, Colleen A. Lambo, Katharine Askelson, Gregory W. Burns, Susanta K. Behura, Thomas E. Spencer, Fuller W. Bazer, Michael Carey Satterfield
Summary: Placental development is modified in response to maternal nutrient restriction, leading to different fetal growth rates. Differences in gene expression and function were found between SGA and NR NonSGA placentomes, with overrepresentation of genes related to natural-killer-cell-mediated cytotoxicity in SGA placentomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pramod Dhakal, Monica Strawn, Ananya Samal, Susanta K. Behura
Summary: The study reveals that uterine Foxa2 has significant effects on gene expression in the fetal brain compared to placenta, with its absence leading to sexually-conflicting transcriptional responses. Genes related to vascular endothelial cells show opposite expression patterns in the fetal brain and placenta of male and female mice lacking uterine Foxa2. This suggests that uterine Foxa2 plays a crucial role in regulating the brain-placental axis by influencing fetoplacental vascular changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yingjun Cui, Susanta K. Behura, Alexander W. E. Franz
Summary: This study generated an initial cell atlas of the adult male and female brain of the mosquito Ae. aegypti using single-nucleus RNA sequencing. The results showed significant differences in cell types and gene expression between the male and female brains. This provides an important foundation for further research on the cellular composition and sexually dimorphic behaviors of mosquito brains.
Article
Cell Biology
Daniela Londono-Vasquez, Katherine Rodriguez-Lukey, Susanta K. Behura, Ahmed Z. Balboula
Summary: Through studying mouse oocytes, researchers discovered a subset of microtubule organizing centers called mcMTOCs that regulate the positioning of the spindle and faithful chromosome segregation during meiosis I. They found that mcMTOCs work in conjunction with F-actin to balance the forces exerted on the spindle and ensure its central anchoring and timely migration.
DEVELOPMENTAL CELL
(2022)
Article
Biology
Constantine A. Simintiras, Jessica N. Drum, Hongyu Liu, M. Sofia Ortega, Thomas E. Spencer
Summary: Research suggests that the uterine lumen fluid (ULF) has a degree of metabolic autonomy, which enhances our understanding of the mechanisms behind pregnancy establishment. ULF plays a crucial role in successful pregnancy establishment and maintenance, impacting the wellbeing of offspring into adulthood. These findings have significant implications for improving fertility and pregnancy outcomes in both domestic animals and women.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maliha Islam, Monica Strawn, Susanta K. Behura
Summary: The sex differences in brain aging are programmed at the fetal stage and epigenetically linked to the placenta.
Article
Developmental Biology
Andrew M. Kelleher, Carolyn C. Allen, Daniel J. Davis, Thomas E. Spencer
Summary: This study describes a new mouse model that is useful for studying uterine gland function during pregnancy. The researchers inserted Cre recombinase into a specific gene using CRISPR-Cas9 genome editing, allowing for targeted expression of Cre recombinase. Additionally, conditional deletion of a key gene in the uterine glands using this mouse model confirmed the value of Prss29-Cre mice in elucidating and exploring adult uterine gland function.
Review
Biochemistry & Molecular Biology
Rodney D. Geisert, Destiny N. Johns, Caroline A. Pfeiffer, Riley M. Sullivan, Caroline G. Lucas, Constantine A. Simintiras, Bethany K. Redel, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather
Summary: CRISPR-Cas9 gene editing technology allows for the study of specific genes involved in reproductive regulation, particularly in the establishment and maintenance of pregnancy. Research on gene editing of porcine conceptuses provides new insights into the regulation of endometrial function, while in vitro studies using endometrial organoids offer an alternative method for studying specific endometrial genes.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Review
Agriculture, Dairy & Animal Science
K. M. Davenport, M. S. Ortega, G. A. Johnson, H. Seo, T. E. Spencer
Summary: Ruminants have a unique placental structure that involves the attachment of binucleate cells to the uterine epithelium to form placental cotyledons. This understanding is crucial in analyzing pregnancy loss and improving pregnancy outcomes in ruminant animals.
Article
Reproductive Biology
Kimberly M. Davenport, Eleanore ONeil, M. Sofia Ortega, Amanda Patterson, Andrew M. Kelleher, Wesley C. Warren, Thomas E. Spencer
Summary: This study utilized single-cell transcriptome analysis to identify different cell types and their ontogeny in bovine conceptus and chorion. The research revealed the role of key transcription factors in the development of trophoblasts and differentiation of binucleate cells. The digital atlas of cell types in the developing bovine conceptus serves as a valuable resource for understanding the genes and pathways involved in its development during critical periods.
BIOLOGY OF REPRODUCTION
(2023)
Article
Developmental Biology
Harriet C. Fitzgerald, Andrew M. Kelleher, Chaman Ranjit, Danny J. Schust, Thomas E. Spencer
Summary: Uterine glands and their secretions play a crucial role in various reproductive processes, such as uterine receptivity, blastocyst implantation, and placental development. Hormones like estrogen and progesterone, as well as external factors, regulate the function of uterine glands. A study using a human endometrial epithelial organoid system found that different proteins secreted by the organoids were modulated by hormone and prostaglandin E2. Additionally, one of the secreted proteins, cystatin C, was shown to attenuate the stromal decidualization response. These findings highlight the impact of uterine gland-derived factors on stromal cell decidualization and its implications for pregnancy establishment and fertility in women.
MOLECULAR HUMAN REPRODUCTION
(2023)
