Article
Biochemistry & Molecular Biology
Jae-Won Hyun, Kibin Lee, Ji-Hun Kim, Dae-Won Sim, Kyu-Tae Byun, Seung-Jae Jung, Jin Chul Paeng, Tae-Bong Kang, Jooho Park, Chan-Gil Kim, Hyung-Sik Won
Summary: The study demonstrates a method for extracellular production of anti-HER2 in Escherichia coli via nonpeptide-guided secretion, enabling purification of the intact protein with high affinity and dissociation constant for potential therapeutic or diagnostic applications in cancer.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Jin-Yuan Chen, Qing-Xia Wei, Liang-Yong Yang, Jia-Yi Li, Tai-Cheng Lu, Zhou-Jie Liu, Guang-Xian Zhong, Xiu-Hua Weng, Xiong-Wei Xu
Summary: This study proposed a multimodal aptasensor for the detection of ochratoxin A (OTA). By using 3'-FAM labeled aptamers and Exo I tool, the aptasensor offers a promising substitute for antibody-dependent biosensors due to its preparatory ease, close affinity, and low cost. However, current aptasensors are mostly limited to single-mode readout and face interference from unbound aptamers and non-target-induced transition events.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoying He, Yan Li, Yehui Tao, Xuelian Qi, Ruiqi Ma, Honghua Jia, Ming Yan, Kequan Chen, Ning Hao
Summary: Sucrose phosphorylase (SPase, EC2.4.1.7) is an important enzyme for producing glycosylated compounds. In this study, an attempt was made to extracellularly express a recombinant SPase from Thermobacillus sp. in E. coli, but the enzyme was unexpectedly produced outside the cells. Truncation experiments showed that both termini of the enzyme are crucial for protein folding and enzymatic activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Oncology
Mengshang Xiong, Ruoqi Liu, Xiaomin Lei, Dongmei Fan, Fangzhen Lin, Wei Hao, Xiangfei Yuan, Yuanyuan Yang, Xiaolong Zhang, Zhou Ye, Yang Lu, Yanjun Zhang, Jianxiang Wang, Dongsheng Xiong
Summary: This study aims to target multiple myeloma by generating BCMA-specific antibodies and constructing CD3/BCMA-directed tandem diabodies. The results demonstrate that CD3/BCMA-directed tandem diabodies have potent and selective anti-MM activities, inhibiting tumor growth.
JOURNAL OF IMMUNOTHERAPY
(2022)
Article
Oncology
Tianhang Zhai, Chao Wang, Yifeng Xu, Weifeng Huang, Zhijun Yuan, Tao Wang, Shuang Dai, Shaogang Peng, Tuling Pang, Wenchao Jiang, Yuhua Huang, Yuefeng Zou, Yingda Xu, Joanne Sun, Xinjiang Gong, Jinping Zhang, Andy Tsun, Bin Li, Xiaoniu Miao
Summary: A unique single-domain antibody targeting the CRD4 domain of 4-1BB was discovered in this study, which showed potent activation of 4-1BB and blockade of PD-L1 in cell-based assays. Incorporation of this antibody into a 4-1BB/PD-L1 bispecific (PM1003) resulted in potent inhibition of PD-L1 activity with minimal toxicity in vivo, making it a promising next generation therapeutic agent for cancer therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Multidisciplinary Sciences
Yiyu Zhang, Da Wei, Xiaochen Wang, Boyi Wang, Ming Li, Haiping Fang, Yi Peng, Qihui Fan, Fangfu Ye
Summary: Microglia are resident macrophage cells in the central nervous system that migrate to search for pathogens or abnormal neural activities. This research reveals that microglia are promptly attracted by external forces, a behavior called mechanotaxis, which has not been confirmed in microglia before. The study also shows that microglial motion shares similarities with bacterial run-and-tumble motion, but with more sophistication, suggesting differences between eukaryotic and prokaryotic cells and their environments. This work provides a framework for studying immune cells exploring complex environments.
Article
Immunology
Ning Shi, Yangyihua Zhou, Yujun Liu, Ran Zhang, Xingjun Jiang, Caiping Ren, Xiang Gao, Longlong Luo
Summary: In this study, a novel bispecific antibody YG-003D3 targeting PD-1 and LAG-3 was designed, showing strong anti-tumor activity by activating immune cells more effectively. The antibody maintained similar affinity and thermal stability to the parental antibody, and could target multiple cells simultaneously to release the 'brake system of immune checkpoints'. The antibody also exhibited better ability to activate PBMC and alter the proportion of immune cells in the tumor microenvironment.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Aleksei K. Misorin, Darya O. Chernyshova, Mikhail S. Karbyshev
Summary: Bispecific antibodies (bsAbs) are highly promising biotherapeutics due to their versatile structure and functional features. They can simultaneously bind two antigens or two epitopes on the same antigen, offering a wide range of clinical applications.
BIOCHEMISTRY-MOSCOW
(2023)
Article
Multidisciplinary Sciences
Rie Jonsson, Alexander Bjorling, Soren Roi Midtgaard, Grethe Vestergaard Jensen, Nicholas Skar-Gislinge, Lise Arleth, Steve Matthews, Karen Angeliki Krogfelt, Havard Jenssen
Summary: This study used small-angle X-ray scattering to reveal the structural morphology of the aggregative adherence fimbriae. The results showed that the fimbriae were arranged in a compact manner with Agg5A + Agg3B subunit proteins assembled pairwise on a flexible string. The study also suggests that the minor subunit plays a significant role in the assembled fimbriae by affecting the overall structure.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Ran You, Jordan Artichoker, Arja Ray, Hugo Gonzalez Velozo, Dan A. Rock, Kip P. Conner, Matthew F. Krummel
Summary: This study characterized the exposure and pharmacodynamic response of an anti-muCD3/anti-huEGFRvIII mouse surrogate BiTE molecule in EGFR variant III-positive breast tumors using intravital imaging. The results revealed heterogeneous temporal and spatial dynamics of BiTE molecule extravasation into solid tumors, indicating physical barriers to its function. High and homogeneous EGFRvIII expression on cancer cells was found to be necessary for efficient tumor clearance by the BiTE molecule. Additionally, the optimal tumor killing required resident tumor-infiltrating lymphocytes (TIL) at high BiTE molecule dosage, while inclusion of peripheral T-cell recruitment was synergistic at moderate to low dosages. Deletion of stimulating conventional type I dendritic cells (cDC1) diminished T-cell activation and tumor clearance induced by the BiTE molecule, suggesting the modulation of in situ antigen-presenting cell (APC) engagements on its efficacy.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yanhui Zheng, Xiaoqing Xu, Xiaoli Fu, Xuerong Zhou, Chao Dou, Yue Yu, Weizhu Yan, Jingyuan Yang, Minqin Xiao, Wilfred A. van der Donk, Xiaofeng Zhu, Wei Cheng
Summary: This study reveals the activation and catalysis mechanisms of the non-heme dioxygen-dependent holoenzyme TglHI during the biosynthesis of 3-thiaglutamate. It also highlights the importance of the biosynthetic enzyme DUF692 family in producing diverse RiPPs.
Article
Oncology
Lisa A. King, Elisa C. Toffoli, Myrthe Veth, Victoria Iglesias-Guimarais, Manon C. Slot, Derk Amsen, Rieneke van de Ven, Sarah Derks, Marieke F. Fransen, Jurriaan B. Tuynman, Thilo Riedl, Rob C. Roovers, Anton E. P. Adang, Jurjen M. Ruben, Paul W. H. I. Parren, Tanja D. de Gruijl, Hans J. van der Vliet
Summary: This study assessed the antitumor activity and safety of a bispecific antibody that activates Vy9V82 T cells. In vitro, in vivo, and ex vivo experiments were conducted, and safety was evaluated in nonhuman primates. The findings demonstrated the potential of the antibody to activate Vy9V82 T cells for antitumor activity and its acceptable safety profile, providing a solid basis for further testing in patients with EGFR-positive malignancies.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Sahar Roshanbin, Ulrika Julku, Mengfei Xiong, Jonas Eriksson, Eliezer Masliah, Greta Hultqvist, Joakim Bergstrom, Martin Ingelsson, Stina Syvanen, Dag Sehlin
Summary: This study investigated a modified bispecific antibody for improved brain uptake in the treatment of Parkinson's disease. The results showed that the antibody could efficiently and quickly penetrate the brain and reduce the aggregation of alpha SYN.
Article
Multidisciplinary Sciences
Arthur Schveitzer Ferreira, Amanda Lopacinski, Michel Batista, Priscila Mazzocchi Hiraiwa, Beatriz Gomes Guimaraes, Nilson Ivo Tonin Zanchin
Summary: Epidermal growth factors (EGF) play important roles in various aspects, including embryogenesis, skin development, and immune response homeostasis. However, recombinant production of these factors in prokaryotic systems is challenging due to their structural organization. This study presents a reliable method for producing seven human growth factors of the EGF family in Escherichia coli, demonstrating their folded and stable conformation and high activity at low concentrations.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Rebecca Faresjo, Gillian Bonvicini, Xiaotian T. Fang, Ximena Aguilar, Dag Sehlin, Stina Syvanen
Summary: This study compared the brain pharmacokinetics of two BBB-penetrating bispecific antibodies of different sizes and found that the smaller non-IgG antibody di-scFv3D6-8D3 exhibited faster elimination from blood, lower brain C-max, higher parenchymal-to-capillary concentration ratio, and earlier brain elimination compared to the larger IgG-based bispecific antibody mAb3D6-scFv8D3. The study also indicated that di-scFv3D6-8D3 had lower avidity towards TfR1, potentially contributing to its faster brain delivery.
FLUIDS AND BARRIERS OF THE CNS
(2021)