Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Article
Neurosciences
Callie M. Weber, Bilal Moiz, Sophia M. Zic, Viviana Alpizar Vargas, Andrew Li, Alisa Morss Clyne
Summary: By studying hpBMEC and hiBMEC, differences in glucose metabolism were found, but overall, the two cell types have similar glucose metabolism. hiBMEC can be used to model cerebral vascular glucose metabolism, expanding their research applications beyond just barrier models.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Article
Multidisciplinary Sciences
Bruno Aristides Bronel, Edgar Maquigussa, Mirian Aparecida Boim, Antonio da Silva Novaes
Summary: This study evaluated the potential beneficial effects of iPSC-derived EV-iPSCs in an in vitro fibrosis model using mouse mesangial cells (MMCs). The results showed that EV-iPSCs effectively reduced inflammation, suppressed RAS activation, and mitigated fibrogenesis.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Tao Li, Kelsey L. Conroy, Amy M. Kim, Julian Halmai, Kewa Gao, Emily Moreno, Aijun Wang, Anthony G. Passerini, Jan A. Nolta, Ping Zhou
Summary: Human induced pluripotent stem cells (hiPSCs) have great potential as a source of vascular cells for therapy and as a model for studying vascular cell differentiation and functionality. This study found that the MEF2C transcription factor was significantly upregulated during the differentiation of vascular progenitors and endothelial cells (ECs) from hiPSCs. Vascular endothelial growth factors (VEGF) induced MEF2C expression in endothelial lineage cells, and this upregulation was dependent on extracellular signal regulated kinase (ERK). Knockdown of MEF2C reduced the migration and tube formation capacity of hiPSC-derived ECs. The study also identified TNF-related apoptosis inducing ligand (TRAIL) and transmembrane protein 100 (TMEM100) as novel targets of MEF2C. These findings highlight the importance of MEF2C in regulating human EC functions and suggest its potential as a therapeutic target for vascular malfunction-associated diseases.
Article
Hematology
Haiyun Pei, Huilin Li, Lei Xu, Bowen Zhang, Heng Zhang, Yali Jia, Liqing Liang, Xiaoyan Xie, Zeng Fan, Zhou Yang, Xiaoling Wang, Feiling Song, Lijuan He, Wen Yue, Xuetao Pei
Summary: The major challenge in using human induced pluripotent stem cells (hiPSCs) is their efficient and controlled differentiation into specific cell types. This study focused on understanding the characteristics of hiPSCs derived from human umbilical arterial endothelial cells (HuA-iPSCs) and their differentiation potential. It was found that HuA-iPSCs retained a transcriptional memory and DNA methylation signature similar to their parental cells, and they showed the highest efficiency in differentiating into hematopoietic cells compared to other hiPSCs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jieun Lee, Hal Sternberg, Paola A. Bignone, James Murai, Nafees N. Malik, Michael D. West, Dana Larocca
Summary: This study presents a panel of clonal embryonic endothelial progenitor cell lines derived from human embryonic stem cells. These cell lines exhibit characteristics of endothelial cells and show strong transcriptomic similarity to adult endothelial primary cell lines. The cells have demonstrated scalability and stable angiogenic properties, making them a potential source for therapeutic cells in cardiovascular disease treatment.
Article
Biochemistry & Molecular Biology
Xuehui Fan, Lukas Cyganek, Katja Nitschke, Stefanie Uhlig, Philipp Nuhn, Karen Bieback, Daniel Duerschmied, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin
Summary: This study compared the electrophysiological and functional properties of endothelial cells derived from human induced pluripotent stem cells with primary human cardiac microvascular endothelial cells. The results demonstrated that the two types of endothelial cells were similar in terms of ion channel function, membrane receptor signaling, and physiological cell functions, although some differences were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Seongjun So, Yoonkyung Park, Soon Suk Kang, Jongsuk Han, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim, Eun Ah Ye, Jae Yong Kim, Hungwon Tchah, Eunju Kang, Hun Lee
Summary: Corneal endothelial cells (CECs) cannot proliferate or recover, causing decreased cell density and loss of pump/barrier function. The study investigated the efficacy and safety of transplanting induced pluripotent stem cell (iPSC)-derived CECs into a corneal endothelial dysfunction (CED) rabbit model. Results showed successful differentiation of iPSCs into CECs and improved corneal transparency after transplantation. iPSC-derived CECs may be a promising cellular resource for treating CED.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Ahmed Faris Aldoghachi, Jit-Kai Loh, Mong-Lien Wang, Yi-Ping Yang, Chian-Shiu Chien, Hui Xin Teh, Alfaqih Hussain Omar, Soon-Keng Cheong, Swee Keong Yeap, Wan Yong Ho, Alan Han-Kiat Ong
Summary: Mesenchymal stem cells (MSCs) derived from adult human tissues have limited proliferative capacity and inconsistent therapeutic outcomes due to their heterogeneous nature. Induced pluripotent stem cells (iPSCs) have emerged as an alternative source, with their ability to differentiate into various specialized cell types. Induced mesenchymal stem cells (iMSCs) derived from iPSCs show superior therapeutic qualities compared to adult MSCs and have potential applications in regenerative medicine. This review focuses on the recent findings of exosomes secreted from iMSCs as a potential noncell-based therapy.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2023)
Article
Biotechnology & Applied Microbiology
Rayyan Gorashi, Nancy Rivera-Bolanos, Caitlyn Dang, Cedric Chai, Beatrix Kovacs, Sara Alharbi, Syeda Subia Ahmed, Yogesh Goyal, Guillermo Ameer, Bin Jiang
Summary: Diabetes is a risk factor for cardiovascular complications due to endothelial dysfunction. In this study, human iPSC-derived endothelial cells were used to create in vitro models of diabetic endothelial dysfunction, replicating the diabetic phenotype in diabetic patient-derived iPSC-ECs. Culturing healthy iPSC-ECs under diabetic conditions induced a diabetic phenotype, while already dysfunctional diabetic iPSC-ECs were unaffected. These models were used to identify angiotensin receptor blockers that improved endothelial function for each patient, demonstrating the potential of iPSC-based models for drug discovery and personalized medicine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Zhewei Gu, Zhiyu Yin, Pengbo Song, Ying Wu, Ying He, Maoshu Zhu, Zhengxin Wu, Sicheng Zhao, Hongri Huang, Huihuang Wang, Cailing Tong, Zhongquan Qi
Summary: Exosomes, as a new cell-free therapy, offer potential advantages for the treatment of diseases by combining the benefits of pluripotent stem cells and the nanoscale size of exosomes. This study investigated the safety and biodistribution of hiPSC-exosomes through cell experiments and animal models, providing support for clinical therapy and research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Letter
Dermatology
Vi Nguyen, Chao Gao, Marcelo L. Hochman, Jacob Kravitz, Elliott H. Chen, Harold Friedman, Camilla F. Wenceslau, Dongbao Chen, Yunguan Wang, J. Stuart Nelson, Anil G. Jegga, Wenbin Tan
Summary: Lesional induced pluripotent stem cell-derived endothelial cells can resemble pathological vascular phenotypes of port-wine birthmark (PWB). Dysregulated pathways, including Hippo and Wnt, NF kappa B, TNF, MAPK, and cholesterol metabolism, were identified in this study. These findings suggest the potential for developing new therapeutics targeting these dysregulated pathways in the treatment of PWB.
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Article
Engineering, Biomedical
Jiesi Luo, Xiangyu Shi, Yuyao Lin, Yifan Yuan, Mehmet H. Kural, Juan Wang, Matthew W. Ellis, Christopher W. Anderson, Shang-Min Zhang, Muhammad Riaz, Laura E. Niklason, Yibing Qyang
Summary: The study successfully developed xenogeneic-free conditions for deriving functional endothelial cells from human induced pluripotent stem cells (XF-hiPSC-ECs), which exhibited similar marker expression and function to human primary ECs. Moreover, the XF-hiPSC-ECs responded to shear stress with typical cell alignment and gene expression, and were successfully used to endothelialize decellularized human vessels in a dynamic bioreactor system, advancing the potential clinical application of tissue engineered vascular grafts.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Xiao Yu Ng, Gary S. L. Peh, Gary Hin-Fai Yam, Hwee Goon Tay, Jodhbir S. Mehta
Summary: Corneal endothelial dysfunction is a major cause of corneal blindness, and the current treatment option is corneal transplantation. However, there is a shortage of suitable donor graft material, leading to the exploration of stem cell-based regenerative medicine using induced pluripotent stem cells (iPSCs). This review discusses the progress and challenges in inducing iPSCs into corneal endothelial cells (CECs), as well as the potential risks and solutions for clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Hui Yang, Lai Wei, Chen Liu, Weiyi Zhong, Bin Li, Yuncan Chen, Rui Han, Jiexian Zhuang, Jianxun Qu, Hongyue Tao, Haiyan Chen, Chen Xu, Qianqian Liang, Chao Lu, Ruizhe Qian, Sifeng Chen, Wenshuo Wang, Ning Sun
ACTA BIOMATERIALIA
(2019)
Article
Cell Biology
Ermin Li, Xiuya Li, Jie Huang, Chen Xu, Qianqian Liang, Kehan Ren, Aobing Bai, Chao Lu, Ruizhe Qian, Ning Sun
Article
Integrative & Complementary Medicine
Sining Wang, Huihua Chen, Yufan Zheng, Zhenyu Li, Baiping Cui, Pei Zhao, Jiali Zheng, Rong Lu, Ning Sun
Article
Cardiac & Cardiovascular Systems
Hing Chung Chan, Yuen Ting Lau, Qianqian Ding, Chun Kit Li, Chi Ming Wong, Pang Chui Shaw, Mary Miu Yee Waye, Suk Ying Tsang
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2020)
Article
Multidisciplinary Sciences
Xinyun Chen, Ying Liu, Chen Xu, Lina Ba, Zhuo Liu, Xiuya Li, Jie Huang, Ed Simpson, Hongyu Gao, Dayan Cao, Wei Sheng, Hanping Qi, Hongrui Ji, Maria Sanderson, Chen-Leng Cai, Xiaohui Li, Lei Yang, Jie Na, Kenichi Yamamura, Yunlong Liu, Guoying Huang, Weinian Shou, Ning Sun
Summary: The RNA-binding protein Quaking (QKI) plays a crucial role in cardiac development and contractile physiology through its regulation of alternative splicing in genes involved in Z-disc formation and contractile physiology. Deficiency of QKI results in the failure of cardiomyocytes to mature into functional cells, highlighting its importance in cardiac pathophysiology.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jie Huang, Yang Liu, Jia-xin Chen, Xin-ya Lu, Wen-jia Zhu, Le Qin, Zi-xuan Xun, Qiu-yi Zheng, Er-min Li, Ning Sun, Chen Xu, Hai-yan Chen
Summary: Harmine exhibits protective effects on cardiac hypertrophy, reducing cell enlargement, restoring calcium handling and contractility, and downregulating hypertrophy-related genes. It also inhibits NF-kappa B phosphorylation and inflammatory cytokines, suggesting it as a promising therapeutic agent for cardiac hypertrophy independent of blood pressure modulation.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Cell & Tissue Engineering
Xianji Liu, Rui Zhao, Qianqian Ding, Xiaoqiang Yao, Suk Ying Tsang
Summary: TRPC7 regulates the automaticity of mESC-CMs through two mechanisms: by positively regulating the intracellular Ca2+ clock through the regulation of activities of RyR2 and SERCA, and by positively regulating the membrane clock via its influence on NCX activity. The expression level of TRPC7 affects the frequency of Ca2+ transients, local Ca2+ releases, and action potentials.
STEM CELL RESEARCH & THERAPY
(2021)
Letter
Cell Biology
Bin Li, Yongkun Zhan, Qianqian Liang, Chen Xu, Xinyan Zhou, Huanhuan Cai, Yufan Zheng, Yifan Guo, Lei Wang, Wenqing Qiu, Baiping Cui, Chao Lu, Ruizhe Qian, Ping Zhou, Haiyan Chen, Yun Liu, Sifeng Chen, Xiaobo Li, Ning Sun
Article
Medicine, General & Internal
Xiuya Li, Fei Gao, Xiaochen Wang, Qianqian Liang, Aobing Bai, Zhuo Liu, Xinyun Chen, Ermin Li, Sifeng Chen, Chao Lu, Ruizhe Qian, Ning Sun, Ping Liang, Chen Xu
Summary: Knockout of the transcription factor E2A increased the proportion of nodal-like cells in hESC-derived CMs, indicating a potential role of E2A in enhancing the efficiency of generating sinoatrial node-like cardiomyocytes from human pluripotent stem cells in the future.
Review
Cell Biology
Qianqian Ding, Yanxiang Qi, Suk-Ying Tsang
Summary: Pluripotent stem cells have the potential to differentiate into cardiomyocytes, but these cells are often considered immature. The process of maturation in cardiomyocytes involves important roles played by mitochondria, including mitochondrial biogenesis, mitophagy, and mitochondrial dynamics.
Article
Cell Biology
Bin Li, Yifan Guo, Yongkun Zhan, Xinyan Zhou, Yongbo Li, Chao Zhao, Ning Sun, Chen Xu, Qianqian Liang
Summary: The deficiency of XIN may play a critical role in the development of DCM induced by TNNT2 mutation, and overexpression of XINB can partially reverse cardiac dysfunction and fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zenghua Qi, Tao Wang, Xiangmao Chen, Chun Kit Wong, Qianqian Ding, Heinrich Sauer, Zhi-Feng Chen, Cheng Long, Xiaoqiang Yao, Zongwei Cai, Suk Ying Tsang
Summary: This study revealed the presence of intracellular Angiotensin II (iAng II) and its regulation of the automaticity of early differentiating cardiomyocytes via the AT(2)R and ryanodine receptor pathways. This provides evidence for the role of iAng II in regulating ESC-CMs behavior and fate determination.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Maxwell Kwok, Carrie Lee, Hung Sing Li, Ruixia Deng, Chantelle Tsoi, Qianqian Ding, Suk Ying Tsang, Kam Tong Leung, Bryan P. Yan, Ellen N. Poon
Summary: The study demonstrated that remdesivir can induce cardiotoxicity in human induced pluripotent stem cell-derived cardiomyocytes at clinically relevant concentrations, including causing mitochondrial damage, reducing redox potential, suppressing mitochondrial respiration, and inducing non-mitochondrial damage, with some of these effects persisting after treatment cessation and leading to cell death.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yufan Zheng, Baiping Cui, Wenrui Sun, Sining Wang, Xu Huang, Han Gao, Fei Gao, Qian Cheng, Limin Lu, Yanpeng An, Xiaobo Li, Ning Sun
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2020)
Article
Medicine, Research & Experimental
Meng Xiang, Meng Lu, Jing Quan, Ming Xu, Dan Meng, Anfeng Cui, Ning Li, Yingying Liu, Peng Lu, Xueling Kang, Xiaokai Wang, Ning Sun, Meng Zhao, Qiujuan Liang, Lili Le, Xinhong Wang, Jianyi Zhang, Sifeng Chen