CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen Blastomyces dermatitidis

Unexpected mutations after CRISPR–Cas9 editing in vivo

(2017) Kellie A Schaefer et al.   NATURE METHODS

Ligation of Dectin-2 with a novel microbial ligand promotes adjuvant activity for vaccination

(2017) Huafeng Wang et al.   PLoS Pathogens

Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

(2016) Daesik Kim et al.   GENOME RESEARCH

The ZIP family zinc transporters support the virulence ofCryptococcus neoformans

(2016) Eunsoo Do et al.   MEDICAL MYCOLOGY

HcZrt2, a zinc responsive gene, is indispensable for the survival ofHistoplasma capsulatum in vivo

(2016) Jessica Dade et al.   MEDICAL MYCOLOGY

DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks

(2016) Megan van Overbeek et al.   MOLECULAR CELL

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases

(2016) Shengdar Q. Tsai et al.   NATURE REVIEWS GENETICS

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering

(2016) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story

(2015) Feng He et al.   Annual Review of Genetics

An evolutionary perspective on zinc uptake by human fungal pathogens

(2015) Duncan Wilson   Metallomics

Codon Bias as a Means to Fine-Tune Gene Expression

(2015) Tessa E.F. Quax et al.   MOLECULAR CELL

New Developments in Blastomycosis

(2015) Jeannina Smith et al.   SEMINARS IN RESPIRATORY AND CRITICAL CARE MEDICINE

Effects of zinc transporters on Cryptococcus gattii virulence

(2015) Rafael de Oliveira Schneider et al.   Scientific Reports

The Dynamic Genome and Transcriptome of the Human Fungal Pathogen Blastomyces and Close Relative Emmonsia

(2015) José F. Muñoz et al.   PLoS Genetics

Sequence Variations and Protein Expression Levels of the Two Immune Evasion Proteins Gpm1 and Pra1 Influence Virulence of Clinical Candida albicans Isolates

(2015) Shanshan Luo et al.   PLoS One

A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi

(2015) Christina S. Nødvig et al.   PLoS One

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

(2014) Richard L Frock et al.   NATURE BIOTECHNOLOGY

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

The ZrfC alkaline zinc transporter is required forAspergillus fumigatusvirulence and its growth in the presence of the Zn/Mn-chelating protein calprotectin

(2013) Jorge Amich et al.   CELLULAR MICROBIOLOGY

A proteomic view of the response of Paracoccidioides yeast cells to zinc deprivation

(2013) Ana Flávia Alves Parente et al.   Fungal Biology

Granulocyte Macrophage-Colony Stimulating Factor Induced Zn Sequestration Enhances Macrophage Superoxide and Limits Intracellular Pathogen Survival

(2013) Kavitha Subramanian Vignesh et al.   IMMUNITY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Zap1 Regulates Zinc Homeostasis and Modulates Virulence in Cryptococcus gattii

(2012) Rafael de Oliveira Schneider et al.   PLoS One

Candida albicans Scavenges Host Zinc via Pra1 during Endothelial Invasion

(2012) Francesco Citiulo et al.   PLoS Pathogens

Limited Model Antigen Expression by Transgenic Fungi Induces Disparate Fates during Differentiation of Adoptively Transferred T Cell Receptor Transgenic CD4+T Cells: Robust Activation and Proliferation with Weak Effector Function during Recall

(2011) Marcel Wüthrich et al.   INFECTION AND IMMUNITY

Vaccine-induced protection against 3 systemic mycoses endemic to North America requires Th17 cells in mice

(2011) Marcel Wüthrich et al.   JOURNAL OF CLINICAL INVESTIGATION

The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway

(2010) Michael R. Lieber   Annual Review of Biochemistry

Aspergillus fumigatus Survival in Alkaline and Extreme Zinc-Limiting Environments Relies on the Induction of a Zinc Homeostasis System Encoded by thezrfCandaspf2Genes

(2009) Jorge Amich et al.   EUKARYOTIC CELL

Immune evasion of the human pathogenic yeast Candida albicans: Pra1 is a Factor H, FHL-1 and plasminogen binding surface protein

(2009) Shanshan Luo et al.   MOLECULAR IMMUNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Neutrophil Extracellular Traps Contain Calprotectin, a Cytosolic Protein Complex Involved in Host Defense against Candida albicans

(2009) Constantin F. Urban et al.   PLoS Pathogens