Article
Biochemical Research Methods
Etienne Laborie, Victor Le-Minh, Thanh Duc Mai, Mehdi Ammar, Myriam Taverna, Claire Smadja
Summary: This study presents a new analytical approach to assess antibody orientation and density on magnetic beads. Results showed that different functionalized beads have varying capture efficiency towards human TNF-alpha immunocapture, with Protein G beads providing the highest efficiency.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Alejandra B. Goya, Danial Baqer, Ryan P. Alexander, Patrycja Stubbs, Karl Dean, Adam M. Lewis, Lewis Coates, Benjamin H. Maskrey, Andrew D. Turner
Summary: Paralytic shellfish toxins were found in Patagonian scallops, while lipophilic and amnesic toxins were below regulatory thresholds. The toxins mainly accumulated in the digestive tracts of scallops, and processing methods effectively reduced the risk of poisonings from final products.
Review
Biochemistry & Molecular Biology
Joana F. Leal, Maria L. S. Cristiano
Summary: Marine biotoxins cause significant socio-economic damage worldwide, with paralytic shellfish toxins being the most severe and potentially lethal. Current knowledge on these toxins is dispersed and partially outdated, necessitating systematic compilation, updating, and complementing. This review focuses on various aspects of these toxins, including molecular structure, bioconversion reactions, newly discovered analogues, and the relationship between molecular structure and toxic activity.
NATURAL PRODUCT REPORTS
(2022)
Article
Biochemical Research Methods
Bas Sleumer, Martijn van Faassen, Michel J. Vos, Rainer Bischoff, Ido P. Kema, Nico C. van de Merbel
Summary: The human growth hormone GH1 (22 kDa) is an important biomarker for growth disorders. A LC-MS/MS method was developed to accurately quantify GH1 and distinguish it from other isoforms. The method showed good performance in different sample types and storage conditions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Najeeb Ullah, Wei Chen, Beenish Noureen, Yulan Tian, Liping Du, Chunsheng Wu, Jie Ma
Summary: An EIS sensor based on MXene nanosheets was developed for STX detection, achieving high sensitivity and good specificity through aptamer modification, with a low detection limit of 0.03 nM. The method demonstrated good selectivity and stability, showing potential for detecting STX in real samples.
Article
Engineering, Environmental
Clare Mills, Michael J. Dillon, Prabir Kumar Kulabhusan, Diana Senovilla-Herrero, Katrina Campbell
Summary: A multiplex lateral flow immunoassay has been developed to detect marine biotoxins, with specific antibodies for each toxin group. The assay is capable of accurately detecting okadaic acid, saxitoxin, and domoic acid within EU regulatory limits. It offers a simple and portable solution for toxin screening in shellfish.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Jingfu Zhao, Wenjing Peng, Xue Dong, Yehia Mechref
Summary: Protein-based therapeutics like mAbs play a crucial role in modern medicine, with glycosylation being a key quality attribute affecting the safety and efficacy of biotherapeutics. An LC-MS/MS-based glycoproteomics approach was used to characterize Fc glycans of two NISTmAb samples, revealing differences in galactosylation levels between the modified and unmodified NISTmAb. Additionally, high abundance glycan structures were identified along with relatively low levels of certain glycans and unglycosylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Xiaoxiao Huang, Xiaobin Xu, Michael A. Partridge, Jihua Chen, Ellen Koehler-Stec, Giane Sumner, Haibo Qiu, Albert Torri, Ning Li
Summary: An urgent need exists for new technologies to characterize immunogenicity to biotherapeutics. In this study, an immunocapture LC-MS assay was developed to determine monkey anti-drug antibody isotypes and semi-quantify them. Results showed that IgG1 was the most abundant isotype in ADA-positive samples, and the LC-MS ADA assay provided additional isotype information compared to traditional ligand binding assays.
Article
Food Science & Technology
Ainhoa Oller-Ruiz, Nuria Alcaraz-Oliver, Gema Ferez, Javier Gilabert
Summary: Marine biotoxins have been a persistent problem along various coasts. This study focused on measuring 16 marine toxins in seawater samples from the Mar Menor coastal lagoon. Only domoic acid was detected, and the study discussed the environmental stressors and mechanisms controlling its presence. The analysis was conducted using solid phase extraction and liquid chromatography-mass spectrometry, with accurate results obtained.
Article
Environmental Sciences
Yadong Cao, Jiangbing Qiu, Aifeng Li, Lei Zhang, Guowang Yan, Ying Ji, Jingrui Zhang, Peng Zhao, Xizhen Wu
Summary: This study investigated the distribution of paralytic shellfish toxins (PSTs) in the coastal waters of Qinhuangdao. Multiple PST components were detected in seawater, sediment, phytoplankton, zooplankton, and various marine organisms. All bivalve shellfish samples contained PSTs, posing a serious threat to human health.
Article
Chemistry, Analytical
Sung Vo Duy, Gabriel Munoz, Quoc Tuc Dinh, Yanyan Zhang, Dana F. Simon, Sebastien Sauve
Summary: In this study, a fast and sensitive analytical method was optimized for the determination of paralytic shellfish poison saxitoxin and its analogues in surface waters. The method involved simple filtration, addition of internal standard, and analysis by online solid-phase extraction coupled to hydrophilic interaction liquid chromatography high-resolution mass spectrometry. The developed method showed good accuracy and precision in both laboratory validation and real sample analysis.
Article
Biochemical Research Methods
Martina Lioi, Sara Tengattini, Francesca Bagatin, Stefano Galliani, Simona Daly, Gabriella Massolini, Caterina Temporini
Summary: Recombinant collagen production using yeasts as expression systems offers a controllable, scalable, and high-quality alternative to traditional extractive methods. However, monitoring procollagen/collagen expression during fermentation can be challenging and time-consuming. In this study, we developed an efficient immunocapture system that can specifically isolate human procollagen type II from fermentation broths and provide detailed characterization for monitoring fermentation processes. The system relies on protein A-coated magnetic beads functionalized with a human anti-procollagen II antibody, which shows high specificity and reusability.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yang Li, Yi Yang, Yinghui Liu, Jinyuan Liu, Yunjia Yang, Jing Zhang, Yue Zou, Lin Shu, Nanyin Han, Bing Shao
Summary: A new method was developed for measuring and diagnosing total IgE in serum for food allergies. This method uses magnetic beads and ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to extract and quantify IgE, and combines in vitro binding experiments to evaluate its binding capacity with different allergenic proteins, thus speculating the allergen causing allergic reactions.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Yang Liu, Zhenfan Chen, Jinxiu Wang, Wei Guo, Chenxiao Zhang, Sen Du, Peng Zhang, Rencheng Yu, Li Zhang
Summary: The study found that the lipophilic marine phycotoxins OA, DTX1, and PTX2 were predominant in the sediments of Jiaozhou Bay in the North Yellow Sea. PTX2 had a more extensive distribution, while OA and DTX1 were more concentrated. The spatial distribution of these three phycotoxins in the sediments was similar due to having the same toxigenic algae origin.
MARINE POLLUTION BULLETIN
(2021)
Article
Chemistry, Applied
Jinnan Chen, Meng Wang, Sen Li, Jin Ye, Li Li, Yu Wu, Di Cai, Tongtong Liu, Lin Zhu, Yi Shao, Songxue Wang
Summary: In this study, a high-throughput automatic sample pretreatment method was developed using immunoaffinity magnetic beads with oriented immobilized antibodies. The pre-column automatic derivatization of HPLC was used to detect grain and feed fumonisin content. The results showed that the capacity of well-oriented antibody immunoaffinity magnetic beads was higher than that of randomly fixed antibody magnetic beads. The method was also successfully applied to analyze FBs in maize samples from different regions in China.
Article
Spectroscopy
Joost L. D. Nelis, J. Pablo Salvador, M. Pilar Marco, Christopher T. Elliott, Katrina Campbell
Summary: An immunoassay utilizing plasmonic coupling between immobilised gold nanorods and colloid gold nanospheres was developed for detecting the marine toxin domoic acid. By optimizing experimental conditions, uniform and high-quality nanorods were obtained, and plasmon coupling was used to detect the target substance.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
P. Nolan, S. Auer, A. Spehar, M. Oplatowska-Stachowiak, K. Campbell
Summary: To enhance global food security, there is a demand for improved monitoring of various toxins throughout the food supply chain. This study investigated the feasibility of using mass-based biosensors for multiplex mycotoxin detection, demonstrating the ability to simultaneously and semi-quantitatively detect three regulated toxins.
Review
Microbiology
Said Oulghazi, Sohaib Sarfraz, Maja A. Zaczek-Moczydlowska, Slimane Khayi, Abdelaziz Ed-Dra, Yassir Lekbach, Katrina Campbell, Lucy Novungayo Moleleki, Richard O'Hanlon, Denis Faure
Summary: The article introduces the characteristics, detection methods, genomics, distribution areas, taxonomical evolution, etc. of Pectobacterium brasiliense (Pbr), emphasizing the importance of its management.
Review
Chemistry, Analytical
Michael Dillon, Maja A. Zaczek-Moczydlowska, Christine Edwards, Andrew D. Turner, Peter I. Miller, Heather Moore, April McKinney, Linda Lawton, Katrina Campbell
Summary: In the past twenty years, the marine biotoxin analysis in routine regulatory monitoring has significantly advanced to high-end analytical techniques from the use of the mouse bioassay. Despite the availability of advanced methods and commercial standards, challenges such as high equipment costs, lengthy procedures, and qualified personnel remain for routine monitoring laboratories.
Article
Chemistry, Analytical
Joost L. D. Nelis, Davide Migliorelli, Lea Muehlebach, Silvia Generelli, Linda Stewart, Christopher T. Elliott, Katrina Campbell
Summary: A novel electrochemical immunosensor was developed for the detection of marine biotoxins DA and OA. The sensor showed excellent stability and sensitivity, achieving low LODs for both toxins in buffer and mussel extract.
Article
Food Science & Technology
Maja A. Zaczek-Moczydlowska, Azadeh Beizaei, Michael Dillon, Katrina Campbell
Summary: This study discusses the importance of rapid on-site testing tools in outbreaks of foodborne diseases, particularly in the detection of norovirus. The advantages and disadvantages of different POCT methods are compared and future trends and directions for POCT methods are examined.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Review
Food Science & Technology
Safiye Jafari, Julian Guercetti, Ariadni Geballa-Koukoula, Aristeidis S. Tsagkaris, Joost L. D. Nelis, M. -Pilar Marco, J. -Pablo Salvador, Arjen Gerssen, Jana Hajslova, Chris Elliott, Katrina Campbell, Davide Migliorelli, Loic Burr, Silvia Generelli, Michel W. F. Nielen, Shana J. Sturla
Summary: Standard methods for chemical food safety testing rely on mass spectrometry in official laboratories, but there is a demand for portable devices due to the need for sampling and expensive equipment. Recent technological advancements include smartphone-based, microfluidic chip-based, and paper-based devices, as well as the potential application of portable mass spectrometers in food testing.
Article
Chemistry, Analytical
M. S. Bacchu, M. R. Ali, S. Das, S. Akter, H. Sakamoto, S- Suye, M. M. Rahman, K. Campbell, M. Z. H. Khan
Summary: An electrochemical DNA biosensor was fabricated for the selective identification of S. Typhi in real samples. The biosensor showed a low limit of detection and the ability to be reused multiple times. The experimental results demonstrated the high feasibility and accuracy of using this biosensor for detecting S. Typhi in clinical and food samples.
ANALYTICA CHIMICA ACTA
(2022)
Review
Biotechnology & Applied Microbiology
Clare Mills, Katrina Campbell
Summary: There is an increasing demand for rapid and affordable in-field screening methods for low molecular weight (LMW) compound detection. This review examines the recent resurgence of using anti-idiotypes (Ab2s) and the potential role of phage display and nanobodies in improving LMW compound immunoassays.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Edel Stone, Vincenzo Pennone, Kerri Reilly, Irene R. Grant, Katrina Campbell, Eric Altermann, Olivia McAuliffe
Summary: The study demonstrated that tailored polyhydroxyalkanoate bionanoparticles (PHA BNPs) displaying recombinant listeriophage enzymes have the ability to lysine and inhibit the growth of Listeria monocytogenes. In laboratory conditions, BNPs displaying only the amidase domain of the phage endolysin were more effective than BNPs displaying the full-length endolysin. However, under conditions that better represent those found in food processing environments, BNPs displaying the full-length endolysin showed a greater inhibitory effect.
Article
Chemistry, Analytical
Safiye Jafari, Loic Burr, Davide Migliorelli, Roger Galve, M. Pilar Marco, Katrina Campbell, Chris Elliott, Michele Suman, Shana J. Sturla, Silvia Generelli
Summary: Considering the complexities and speed of modern food chains, there is an increasing demand for point-of-need detection of food contaminants, particularly highly regulated chemicals and carcinogens such as aflatoxin B1. We report a user-friendly smartphone-based magneto-immunosensor on carbon black modified electrodes for point-of-need detection of aflatoxin B1 in cereals. The assay demonstrated a low limit of detection and high reproducibility, and its applicability was validated on the basis of EU guidelines. An open-source android application, AflaESense, was designed to provide a simple interface for data analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Engineering, Environmental
Clare Mills, Michael J. Dillon, Prabir Kumar Kulabhusan, Diana Senovilla-Herrero, Katrina Campbell
Summary: A multiplex lateral flow immunoassay has been developed to detect marine biotoxins, with specific antibodies for each toxin group. The assay is capable of accurately detecting okadaic acid, saxitoxin, and domoic acid within EU regulatory limits. It offers a simple and portable solution for toxin screening in shellfish.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Water Resources
Andrew A. Meharg, Caroline Meharg, Manus Carey, Paul Williams, Zhengyu Shi, Katrina Campbell, Christopher Elliott, Ernest Marwa, Xiao Jiujin, Julia Gomes Farias, Fernando Teixeira Nicoloso, P. Mangala C. S. De Silva, Ying Lu, Andy J. Green, Eduardo Moreno-Jimenez, Angel Antonio Carbonell-Barrachina, Alessia Sommella, Massimo Pigna, Catherine Brabet, Didier Montet, Mahmud Hossain, M. Rafiqul Islam
Summary: Despite the lack of understanding about the variation and correlation of elements in staple grains, a comprehensive analysis of polished rice samples from 18 countries revealed major differences in elemental content based on geographic location, with some elements strongly associated while others showing weaker correlations.
EXPOSURE AND HEALTH
(2023)
Article
Food Science & Technology
Sim Ray Yue, Rajeev Shrivastava, Katrina Campbell, Michael John Walker
Summary: This study analyzed allergen food recalls issued by food regulatory bodies in the UK from 2016 to 2021. The analysis included food type, allergenic food group, reasons for recall, and food expiry status. The findings revealed an increasing trend in allergen-related recalls until 2019, with a peak of 118 recalls, followed by a decrease to 82 and 84 recalls in 2020 and 2021 respectively. Milk was the most recalled allergenic food group, accounting for 25.2% of recalls. The omission of priority allergens from the list of ingredients was the main reason for recalls, and the supermarket Lidl issued the most recalls with 37 recalls involving 62 products. Some recalls with expiry dates posed microbiological risks to consumers, especially in cereal & bakery products.
Review
Peripheral Vascular Disease
Saad Mahjub Atiku, Dennis Kasozi, Katrina Campbell
Summary: This study explored the prevalence of single nucleotide polymorphisms (SNPs) in ACE1 and ACE2 in different geographic locations and their association with COVID-19 prevalence. The DD genotype of ACE1 was found to be more prevalent in Europe and America, which had a higher impact of COVID-19 compared to Asia and Africa. The rs2285666 SNP of ACE2 varied in different locations. However, the association between ACE2 and COVID-19 remains inconclusive and further research is needed.
JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM
(2023)
