期刊
ENVIRONMENTAL MICROBIOLOGY REPORTS
卷 10, 期 1, 页码 102-107出版社
WILEY
DOI: 10.1111/1758-2229.12615
关键词
-
资金
- European Commission (Marie Curie ITN NORA) [FP7-316472]
N2O is a potent greenhouse gas, but also a potent electron acceptor. In search of thermodynamically favourable - yet undescribed - metabolic pathways involving N2O reduction, we set up a continuous microbial enrichment, inoculated with activated sludge, fed with N2O as the sole electron acceptor and acetate as an electron donor. A nitrogen-free mineral medium was used with the intention of creating a selective pressure towards organisms that would use N2O directly as source of nitrogen for cell synthesis. Instead, we obtained a culture dominated by microorganisms of the Rhodocyclaceae family growing by N2O reduction to N-2 coupled to N-2 fixation. Biomass yields of this culture were 40% lower than those of a previously reported culture grown under comparable conditions but with an NH4+-amended medium, as expected from the extra energy expense of N-2 fixation. Interestingly, we found no significant difference in yields whether N2O or acetate was the growth-limiting substrate in the chemostat in contrast to the study with NH4+-amended medium, in which biomass yields were roughly 30% lower during acetate limiting conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据