期刊
CHEMICAL SCIENCE
卷 9, 期 15, 页码 3672-3676出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8sc00733k
关键词
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资金
- EPSRC
- University of Bath
- National Natural Science Foundation of China [21722801, 21572058]
- Science and Technology Commission of Shanghai Municipality [15540723800]
- Fundamental Research Funds for the Central Universities [222201717003]
- Shanghai Rising-Star Program [16QA1401400]
- Royal Society
- ECUST
We have developed a novel AND logic based fluorescence probe for the simultaneous detection of ONOO- and GSH (GSH-PF3). The GSH-PF3 probe was synthesised over three steps starting from commercially available fluorescein. The probe was constructed by attaching the GSH reactive motif, 2,4-dinitrobenzenesulfonyl, to the previously reported boronate fluorescence based probe, PF3. GSH-PF3 produced only a small fluorescence response towards the addition of GSH or ONOO- separately. However, when the probe was exposed to both analytes, there was a significant (40-fold) fluorescence enhancement. GSH-PF3 demonstrated an excellent selectivity towards both GSH and ONOO-. In cellular imaging experiments the probe was shown to be cell permeable with no 'turn-on' response observed for the addition of either GSH or ONOO- separately. However, in the presence of both analytes, a clear fluorescence response was observed in live cells. GSH-PF3 was further able to monitor the co-existence of metabolically produced ONOO- and GSH by exogenous stimulation.
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