Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Qianying Zhou, Pengying Liang, Ting Huang, Mingqi Guo, Baoping Xie, Chunrong Li, Minmin Li, Wen-Jun Duan, Jin-Xiang Chen, Zong Dai, Jun Chen
Summary: An endogenous enzyme-powered DNA nanomotor (EEPDN) was proposed for imaging miRNA in living cells. The motor system, constructed based on gold nanoparticles, utilizes APE1 enzyme as fuel for repetitive motion cycles, achieving highly sensitive miRNA imaging. The strategy also successfully differentiated the expression of specific miRNA between tumor cells and normal cells, demonstrating high tumor cell selectivity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xiao Zhao, Wenfang Deng, Yueming Tan, Qingji Xie
Summary: By varying the concentration of microRNA-141, the amount of glucose oxidase immobilized on the bioanode can be easily controlled, allowing for sensitive detection of microRNA-141 using a glucose/O-2 biofuel cell as a self-powering sensor.
CHEMICAL COMMUNICATIONS
(2022)
Review
Chemistry, Analytical
Aijiao Yuan, Hang Xiao, Fan Yang, Huifang Hao, Xiaoyan Wang, Jinhua Li, Minghua Jin, Qiang Zhao, Rui Sha, Zhaojia Deng, Hanyong Peng
Summary: The rapid development of DNA nanotechnology allows for the programmable operation of advanced DNA walkers, which mimic the function of protein motors in living organisms. These walkers require four main components for construction, including motor, track, energy, and cargo, and can autonomously perform specific functions following a predesigned trajectory with nanometer precision. Recent research has focused on improving the progressivity, speed, and directionality of DNA walkers, and new DNA nano techniques have shown powerful signal amplification during their operation. This review covers the breakthrough design and principle of walking systems and highlights the recent application of DNA walkers for signal amplification in living cells. The engineered DNA walkers have great potential for diverse applications, such as imaging intracellular molecules, modulating cellular functions, and facilitating drug delivery.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Hui Hu, Fu Zhou, Baojuan Wang, Xin Chang, Tianyue Dai, Ruifen Tian, Yifei Wan, Xiayan Wang, Guangfeng Wang
Summary: This study demonstrates a newly updated autonomously operated and highly integrated 3D DNA walker in a tumor microenvironment, which successfully amplified signals and performed miRNA imaging bioanalytical tasks in living cells.
Article
Biophysics
Bin-Cheng Yin, Bang-Ce Ye, Jin-Yu Wang, Hua-Dong Li, Pei-Qiang Ma, Ying Zhou
Summary: We developed a DNA nanomachine initiated by a miRNA-induced silencing complex (miRISC) for imaging miRNA activity in living cells. The nanomachine was activated by the miRISC-mediated RNA cleavage reaction, resulting in autonomous movement and release of fluorescently labeled DNA strands. It successfully detected miR-21 activity in different cell lines and showed versatility in detecting other miRNAs. Compared to existing methods, this nanomachine has advantages in terms of simplicity, analysis time, and signal output. It expands the application of DNA nanomachine in miRNA activity detection and has potential for basic research and clinical biomedicine.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Mengxi Chen, Ruilin Duan, Shijun Xu, Zhijuan Duan, Quan Yuan, Fan Xia, Fujian Huang
Summary: A photoactivated DNA walker signal amplification strategy was developed for photocontrollable imaging of cancer-related miRNA in single living cells. The method allows on-demand activation of the DNA walker for specified signal amplification imaging of cancer-related miRNA.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuetong Li, Yuxin Xie, Yue Zhang, Hongxia Zhao, Huangxian Ju, Ying Liu
Summary: A photo-locked DNA nanomachine (P-DNA nanomachine) is designed in this study, which uses single NIR irradiation for spatial and temporal activation of the nanomachine's operation and metal ion imaging. This nanomachine demonstrates effective protection against false positive signals from extracellular interference and has potential for in vivo imaging applications.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Lu-Ying Duan, Jin-Wen Liu, Ru-Qin Yu, Jian-Hui Jiang
Summary: DNA molecular probes are powerful tools for fluorescence imaging of miRNAs in living cells. A novel DNA nanomachine has been developed to amplify sensing and imaging of tumor-related miR-21 with dynamic responses, excellent sensitivity and selectivity. This nanoplatform shows potential for accurate monitoring of miR-21 expression levels for tumor diagnosis and biological research.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Selcuk Yavuz, Helene Kabbech, Jente van Staalduinen, Simon Linder, Wiggert A. van Cappellen, Alex L. Nigg, Tsion E. Abraham, Johan A. Slotman, Marti Quevedo, Raymond A. Poot, Wilbert Zwart, Martin E. van Royen, Frank G. Grosveld, Ihor Smal, Adriaan B. Houtsmuller
Summary: This study reveals the formation of visible foci of androgen receptors (ARs) in the nucleus upon stimulation by testosterone, as well as the potential compartmentalization of these foci through liquid-liquid phase separation. Furthermore, it demonstrates the presence of AR-regulated genes within these foci.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Medicine, Research & Experimental
Fei Wang, Ling Sum Liu, Pan Li, Hoi Man Leung, Dick Yan Tam, Pik Kwan Lo
Summary: This article presents a TNA-based probe for real-time monitoring of target miRNA levels in cells, highlighting its high specificity, selectivity, and stability.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Biophysics
Yamin Nie, Jie Jiang, Kan-fu Peng, Ya-qin Chai, Ruo Yuan
Summary: A bidirectional one-dimensional DNA walking nanomachine was developed in this study, which could move two different DNA walkers in opposite directions along a track and release payload simultaneously. This concept not only improves locomotion efficiency but also has the potential to facilitate the development of DNA dynamic nanomachines and intelligent nanosensors.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xia Li, Fang Yang, Shunmei Li, Ruo Yuan, Yun Xiang
Summary: This study demonstrates the synthesis of a new DNA nanocage framework (DNF) for sensitive monitoring of mature miRNA-21 in living cells. The DNF has the capability to selectively discriminate mature miRNA-21 from precursor miRNA and can amplify fluorescence signals for high accuracy detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Hao Fan, Yani He, Qingxia Shu, Xinru Wang, Hanfeng Cui, Yuping Hu, Guobing Wei, Huanhuan Dong, Jing Zhang, Nian Hong
Summary: In this study, catalyzed hairpin assembly is used as an automated strategy to detect specific target DNA in living cells. By connecting the fuel and starting chain through an auxiliary chain, a triple-stranded DNA is formed to complete a single system. Multiple single systems modified on gold nanoparticles act as DNA orbitals, enabling the automatic walking of a three-dimensional fluorescence machine through base complementation.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sha Yu, Yang Zhou, Yao Sun, Shaojun Wu, Tingting Xu, Yu-Chung Chang, Sai Bi, Li-Ping Jiang, Jun-Jie Zhu
Summary: The study developed a nanomachine for breast cancer treatment, which can be activated inside cells for in situ imaging and targeted synergistic therapy, including releasing fluorophores and aggregating AuNPs for imaging, suppressing survivin expression, and photothermal therapy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Guo Ge, Tianlin Wang, Zihou Liu, Xiaomeng Liu, Tiange Li, Yuntang Chen, Jialin Fan, Erkigul Bukye, Xianqing Huang, Lianjun Song
Summary: A fluorescent aptamer sensor based on self-assembled DNA double-crossover was developed for simultaneous quantitative detection of aflatoxins M1 and B1. The sensor showed good linearity and wide linear range under optimal conditions, with detection limits of 6.24 pg/mL and 9.0 pg/mL, respectively. It also exhibited good interference immunity and potential for high-throughput screening of other analytes.
Article
Chemistry, Multidisciplinary
Pei-Qiang Ma, Qing Huang, Hua-Dong Li, Bin-Cheng Yin, Bang-Ce Ye
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Biochemical Research Methods
Wenping Wei, Yanzhe Shang, Ping Zhang, Yong Liu, Di You, Bincheng Yin, Bangce Ye
ACS SYNTHETIC BIOLOGY
(2020)
Article
Materials Science, Biomaterials
Shi-Fu Liang, Fang-Fang Zuo, Bin-Cheng Yin, Bang-Ce Ye
Summary: This paper presents the potential use of Angiopep-2 (An2)-functionalized siRNA-loaded exosomes as a therapeutic agent for glioblastoma treatment. The experimental results demonstrate the superior properties of Exo-An2-siRNA, including high blood stability, efficient cellular uptake, and outstanding blood-brain barrier penetration ability.
BIOMATERIALS SCIENCE
(2022)
Article
Chemistry, Analytical
Ting Wang, Yan Wang, Pinru Chen, Bin-Cheng Yin, Bang-Ce Ye
Summary: Structure-guided protein engineering was used to improve the kinetics and targeting specificity of Cas9n, leading to the development of a new RNA detection method. This method offers high sensitivity and the ability to directly detect RNA, with great potential for various biological applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Peipei Qin, Pinru Chen, Nan Deng, Liu Tan, Bin-Cheng Yin, Bang-Ce Ye
Summary: This study proposes a method to directly regulate the trans cleavage activity of Cas12a using an allosteric DNA probe, enabling the sensing of different types of analytes. The proposed strategy utilizes an allosteric inhibitor probe that can induce a change in conformation and remove its inhibitory function on Cas12a upon binding to a specific target. The method was validated for detecting adenosine triphosphate and let-7a, demonstrating excellent specificity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Bin-Cheng Yin, Bang-Ce Ye, Jin-Yu Wang, Hua-Dong Li, Pei-Qiang Ma, Ying Zhou
Summary: We developed a DNA nanomachine initiated by a miRNA-induced silencing complex (miRISC) for imaging miRNA activity in living cells. The nanomachine was activated by the miRISC-mediated RNA cleavage reaction, resulting in autonomous movement and release of fluorescently labeled DNA strands. It successfully detected miR-21 activity in different cell lines and showed versatility in detecting other miRNAs. Compared to existing methods, this nanomachine has advantages in terms of simplicity, analysis time, and signal output. It expands the application of DNA nanomachine in miRNA activity detection and has potential for basic research and clinical biomedicine.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Pei-Qiang Ma, Tian-Xian Liu, Hua-Dong Li, Bin-Cheng Yin, Bang-Ce Ye
Summary: This study developed a non-genetic T-cell engineering strategy to enhance the specificity and efficacy of cancer therapy. The nano-biohybrid DNA engager-reprogrammed T-cell receptor system allows for precise control of T-cell response and mitigates the risks associated with genetic engineering. The effectiveness of the system was demonstrated in tumor models.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Hua-Dong Li, Pei-Qiang Ma, Jin-Yu Wang, Bin-Cheng Yin, Bang-Ce Ye
Summary: Inspired by social biotic colonies, a DNA nanodevice community is proposed as a universal and scalable platform. The modular nanodevice establishes an inter-nanodevice communication network, enabling diverse tasks such as signal cascading and feedback, distributed logic computing, and virus transmission simulation.
Article
Chemistry, Medicinal
He-Hua Zhang, Jian Xiang, Bin-Cheng Yin, Bang-Ce Ye
Summary: Multidrug resistance (MDR) is a major challenge in cancer chemotherapy due to the ability of ABC transporters to efflux antitumor drugs from cells. This study demonstrates the use of a cytosine base editor (CBE) system to knock out genes encoding ABC transporters, resulting in reduced expression and increased drug retention in MDR cells. The CBE system shows potential for targeting different ABC efflux transporters and restoring chemosensitivity in MDR cancer cells. These findings provide valuable insights for using CRISPR technology to overcome MDR in cancer cells.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Chemistry, Analytical
Meng-Li Chen, Fang He, Bin-Cheng Yin, Bang-Ce Ye
Summary: The DNA-engineered exosome (DNA-Exo) nanoplatform allows for imaging dual cancer biomarkers at the single-cell level, enabling convenient dual-fluorescence imaging of cancer cells. This method demonstrates the capacity to visually discriminate different cell types based on the specific recognition of analytes.
Article
Nanoscience & Nanotechnology
Qing Huang, Pei-Qiang Ma, Hua-Dong Li, Bin-Cheng Yin, Bang-Ce Ye
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Analytical
Huan-Huan Sun, Fang He, Ting Wang, Bin-Cheng Yin, Bang-Ce Ye
Article
Chemistry, Analytical
Cui-Fang Ning, Luying Wang, Ya-Fei Tian, Bin-Cheng Yin, Bang-Ce Ye
Article
Chemistry, Multidisciplinary
Alexander M. Prophet, Kritanjan Polley, Gary J. Van Berkel, David T. Limmer, Kevin R. Wilson
Summary: The oxidation kinetics of iodide by ozone at the air-water interface is studied in single microdroplets. Molecular simulations and kinetic modeling are used to understand the underlying multiphase mechanism.
Article
Chemistry, Multidisciplinary
Rong Zhang, Jiajing Lan, Fei Wang, Shumei Chen, Jian Zhang
Summary: By utilizing 1,1'-ferrocene dicarboxylic acid as a chelating and surface protection ligand, we have synthesized multi-nuclear indium oxide clusters with varying nuclear sizes, including heptanuclear and thirteen-nuclear clusters. These clusters possess labile coordination sites, allowing for structural modification and self-assembly, resulting in the formation of various cluster structures.
Article
Chemistry, Multidisciplinary
Hui Wang, Hui Liu, Mingsen Wang, Jiaheng Hou, Yongjun Li, Yuancheng Wang, Yingjie Zhao
Summary: Two supramolecular complexes were prepared using CBs and M1, and their single-crystal structures were analyzed by SCXRD. The unexpected 1:2 self-assembly structure between M1 and CB[8] was discovered for the first time. These complexes exhibit unique photophysical properties and provide valuable information about the structure and photophysical properties of supramolecular complexes.
Article
Chemistry, Multidisciplinary
Chang-Hee Lee, Sookil Park, Sanggil Kim, Ji Young Hyun, Hyun Soo Lee, Injae Shin
Summary: The epidermal growth factor receptor (EGFR) is a cell-surface glycoprotein involved in cell proliferation and tumor development. This study used a fluorescently labeled EGFR to investigate its time-dependent endocytosis in live cells and found that appended glycans affect EGFR internalization. Additionally, the study detected sialic acid residues attached to EGFR on the live cell surface using FRET-based imaging. This research provides valuable insights into the cellular functions of EGFR.
Article
Chemistry, Multidisciplinary
Subhankar Sahu, Lokesh Kumar, Sumita Das, Dipti Gupta, Ruchi Anand
Summary: This study proposes a strategy that combines organic electronics with biosensor scaffolds to create a compact device for monitoring environmental aromatic pollution. By coupling biosensing protein MopR with an organic electrochemical transistor (OECT), a sensor module capable of efficient detection of phenol was designed. Exclusive phenol detection with minimal loss of sensitivity could be achieved in complex pollutant mixtures and real environmental samples.
Review
Chemistry, Multidisciplinary
Changseok Lee, Hyung-Joon Kang, Sungwoo Hong
Summary: The formation of C-N bonds through hydroamination reactions catalyzed by nickel hydrides has been a topic of recent interest. This approach offers a way to efficiently transform a variety of alkene and alkyne substrates into compounds enriched with C-N bonds. The review provides a concise overview of the underlying reaction mechanisms and aims to stimulate further progress in NiH-catalytic techniques and catalyst design.
Article
Chemistry, Multidisciplinary
Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James
Summary: Drug-induced liver injury (DILI) is a common cause of acute liver failure in the USA and Europe, but most cases can be recovered or prevented by discontinuing the offending drug. Recent research has found that peroxynitrite (ONOO-) can be used as a potential indicator for early diagnosis of DILI, and there is an urgent need to establish a method to detect and track peroxynitrite in DILI cases. In this study, a FRET-based nano fluorescent probe CD-N-I was developed, which showed high selectivity and sensitivity in detecting peroxynitrite. The probe successfully detected exogenous peroxynitrite in live cells and endogenous peroxynitrite in APAP-induced liver injury of HepG2 cells.
Article
Chemistry, Multidisciplinary
Dmitry L. Lipilin, Mikhail O. Zubkov, Mikhail D. Kosobokov, Alexander D. Dilman
Summary: This article describes a direct photocatalytic method for the thiolation of unprotected acids, which was previously challenging. By using a thionocarbonate reagent with an N-O bond, the efficient conversion of carboxylic acids to thiols is achieved.
Article
Chemistry, Multidisciplinary
Jason Malenfant, Lucille Kuster, Yohann Gagne, Kouassi Signo, Maxime Denis, Sylvain Canesi, Mathieu Frenette
Summary: Raman microscopy can reveal compound-specific vibrational fingerprints without sample preparation. The combination of efficient theoretical calculations and a user-friendly software can accurately predict peak positions and provide match scores to assist with structure determination.
Article
Chemistry, Multidisciplinary
Jayoh A. Hernandez, Paul S. Micus, Sean Alec Lois Sunga, Luca Mazzei, Stefano Ciurli, Gabriele Meloni
Summary: Essential trace metals play crucial roles in the survival and virulence of bacterial pathogens. Helicobacter pylori requires nickel for colonization and persistence in the stomach, and NixA is an essential nickel transporter in this process. This study characterizes the selectivity and electrogenic nature of NixA-mediated nickel transport.
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Jacqueline R. Santhouse, Jeremy M. G. Leung, Lillian T. Chong, W. Seth Horne
Summary: By studying the folding kinetics and mechanism of the BdpA sequence, researchers found that altering the backbone connectivity can affect protein folding. This suggests that protein mimetic chains have a significant degree of plasticity in transitioning between unfolded and folded states.
Article
Chemistry, Multidisciplinary
Divanshu Gupta, Ralf Einholz, Holger F. Bettinger
Summary: This study presents the first direct spectroscopic evidence of a cyclic seven-membered iminoborane. Compared to linear amino-iminoboranes, this cyclic iminoborane exhibits weakened bond strength and lower Lewis acidity value. The study suggests that the reduced ring strain of cyclic iminoborane prevents nitrogen fixation but allows facile (2 + 2) cycloaddition reaction with C2H4.
Article
Chemistry, Multidisciplinary
Renny Mathew, Aniruddha Mazumder, Praveen Kumar, Julie Matula, Sharmarke Mohamed, Petr Brazda, Mahesh Hariharan, Brijith Thomas
Summary: This study reveals the packing arrangement of partially disordered nitro-perylenediimide (NO2-PDI) using a synergistic approach that combines 3D ED, ssNMR, and DFT techniques. By overcoming these challenges, this methodology opens up new avenues for material characterization, driving exciting advancements in the field.
