Article
Biochemical Research Methods
Jonathan M. Matthews, Brooke Schuster, Sara Saheb Kashaf, Ping Liu, Rakefet Ben-Yishay, Dana Ishay-Ronen, Evgeny Izumchenko, Le Shen, Christopher R. Weber, Margaret Bielski, Sonia S. Kupfer, Mustafa Bilgic, Andrey Rzhetsky, Savas Tay
Summary: Organoids are promising ex vivo disease models for drug discovery, but analyzing their morphology, number, and size is challenging. This study presents OrganoID, an image analysis platform that automatically recognizes, labels, and tracks single organoids pixel-by-pixel. OrganoID enables straightforward and accurate image analysis to accelerate the use of organoids in biomedical applications.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Ilie Valentin Mihai, Andreea Constantin, Stefania Bucuci, Razvan D. Tamas
Summary: This paper proposes a single-antenna technique for measuring the radar cross-section in a real environment. The technique corrects the near-field radar cross-section using an analytical factor calculated based on the far-field and near-field cross-sections. It takes into account the diffraction effects at the edges of the target for incidence angles higher than 20 degrees. The method also includes distance averaging and time-gating procedures to mitigate multipath propagation effects and isolate the reflection from the target.
Article
Biology
Galina Popova, Hanna Retallack, Chang N. Kim, Albert Wang, David Shin, Joseph L. DeRisi, Tomasz Nowakowski
Summary: This study reveals that rubella virus predominantly infects microglia in developing brain, and the infection leads to a profound interferon response in non-microglial cells, affecting fetal neurodevelopment.
Article
Chemistry, Multidisciplinary
Intan Rosalina Suhito, Jin Won Kim, Kyeong-Mo Koo, Sun Ah Nam, Yong Kyun Kim, Tae-Hyung Kim
Summary: A new method is proposed for the real-time assessment of kidney organoids, allowing non-destructive evaluation of differentiation and maturation levels. This method utilizes electrochemical signals to differentiate kidney cells from off-target cells, providing a promising tool for organoid research.
Article
Multidisciplinary Sciences
Chong Li, Jonas Simon Fleck, Catarina Martins-Costa, Thomas R. Burkard, Jan Themann, Marlene Stuempflen, Angela Maria Peer, Abel Vertesy, Jamie B. Littleboy, Christopher Esk, Ulrich Elling, Gregor Kasprian, Nina S. Corsini, Barbara Treutlein, Juergen A. Knoblich
Summary: The study develops a high-throughput CRISPR screening system in cerebral organoids to identify vulnerable cell types and gene regulatory networks associated with autism spectrum disorder. The researchers found that perturbing members of the chromatin remodelling complex can affect cell fate determination in progenitor cells.
Article
Engineering, Electrical & Electronic
Rachel E. Jarvis, Rylee G. Mattingly, Jay W. McDaniel
Summary: The article discusses the challenges and advantages of measuring radar cross section (RCS) using a single-antenna test setup, highlighting the use of traditional RF measurement hardware and postprocessing algorithms to accurately extract RCS. Experimental results showed an initial average error of 4.9 dBsm over the 300-1000 MHz frequency range using only calibration and vector background subtraction, which was further reduced to 0.54 dBsm after applying time gating.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Crystallography
Andrei Rogalev, Fabrice Wilhelm, Elena Ovchinnikova, Aydar Enikeev, Roman Bakonin, Ksenia Kozlovskaya, Alexey Oreshko, Dai Aoki, Vladimir E. Dmitrienko
Summary: The absorption spectra of a single CeCoGe3 crystal under two orthogonal linearly polarized X-rays near the K-edges of Ge, Co, and the L-23 edges of Ce were measured at the ID12 beamline of the ESRF. X-ray natural linear dichroism (XNLD) was observed near all absorption edges, indicating a splitting of electronic states in a crystalline field. Mathematical modeling and comparison with experimental data allowed determination of the isotropic and anisotropic parts of atomic absorption cross section in CeCoGe3 near all measured absorption edges, showing different anisotropic contributions from Ge atoms in two different Wyckoff positions.
Article
Nuclear Science & Technology
Satoshi Takeda, Ryosuke Shibano, Takanori Kitada
Summary: A cross-section adjustment method based on Bayesian theory is derived for adjusting specific cross-sections, which reduces the uncertainty of the multiplication factor significantly compared to conventional methods.
JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qinfeng Ma, Haodong Tao, Qiang Li, Zhaoyu Zhai, Xuelu Zhang, Zhewei Lin, Ni Kuang, Jianbo Pan
Summary: OrganoidDB is a comprehensive resource for exploring the transcriptomes of organoids. It contains a large amount of organoid data and allows for different modes of queries and comparisons. This will help us better understand organoids and improve organoid culture protocols.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Oncology
Daniele Cattaneo, Giorgio Alberto Croci, Cristina Bucelli, Silvia Tabano, Marta Giulia Cannone, Gabriella Gaudioso, Maria Chiara Barbanti, Kordelia Barbullushi, Paola Bianchi, Elisa Fermo, Sonia Fabris, Luca Baldini, Umberto Gianelli, Alessandra Iurlo
Summary: The lack of mutations in driver genes JAK2, CALR, or MPL in BCR-ABL1-negative myeloproliferative neoplasms is known as a triple-negative genotype, which is found in a subset of patients with essential thrombocythemia and primary myelofibrosis. This study aimed to evaluate the clinical and molecular profile of triple-negative ET patients using next-generation sequencing, identifying potential clonal biomarkers. Most cases of triple-negative ET showed clonal diseases based on NGS, suggesting the need for tailored treatment strategies for this subset of patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Engineering, Electrical & Electronic
Lei Gan, Wen Jiang, Qiang Chen, Xiaoqiu Li, Zhipeng Zhou, Shuxi Gong
Summary: A new method is proposed to estimate the AM-RCS of a large-scale array antenna by improving the SPMM to consider mutual coupling and edge effects, thereby increasing accuracy. The AM-RCS of a large-scale array antenna is estimated by deducing it from the subarray, with the accuracy of the method improving as the subarray size increases. Verification using microstrip linear array and Vivaldi planar array shows that the proposed method has lower calculated error and memory requirement compared to the reference method.
IEEE TRANSACTIONS ON ANTENNAS AND PROPAGATION
(2021)
Article
Computer Science, Interdisciplinary Applications
Rong Du, Xiao-Xiao Cai
Summary: The Gaussian approximation is often used to calculate neutron incoherent inelastic scattering functions in liquids due to its accuracy in both short and long time limits. However, additional physical approximations are commonly employed to overcome numerical difficulties in the evaluation process. In this study, a new numerical method called convolutional discrete Fourier transform is proposed to perform Fourier transform of exp[- f (t)]. The results obtained from applying this method to computing the differential cross sections of light water up to 10 eV showed a higher dynamic range compared to conventional fast Fourier transform. The calculated integral cross sections closely matched the data in the state-of-the-art nuclear data library for light water. The numerical method proposed in this study can be a replacement for the extra physical approximations used.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Chemistry, Analytical
Dmitry A. Baklykov, Mihail Andronic, Olga S. Sorokina, Sergey S. Avdeev, Kirill A. Buzaverov, Ilya A. Ryzhikov, Ilya A. Rodionov
Summary: A highly controllable cross-section cleaving method is proposed to minimize the impact on the resulting deep etching profile. Experimental comparison of two cleaving methods based on different auxiliary microstructures is conducted to analyze the interaction between the auxiliary lines and the etching process.
Article
Nuclear Science & Technology
Matthias H. Nezondet, Willem F. G. van Rooijen
Summary: This paper introduces a neutron transport calculation code based on the Iso-Geometric Analysis (IGA) method and emphasizes the need for self-shielding calculations using the subgroup method. The basic theory of the IGA method is presented, and the validation of the subgroup method for IGA and S-N transport calculations is demonstrated.
PROGRESS IN NUCLEAR ENERGY
(2022)
Article
Construction & Building Technology
Zhenyu Sun, Dingli Zhang, Qian Fang
Summary: This paper reviews the progress made by our research team in constructing large cross-section subsea tunnels using the drilling and blasting method. It introduces our research on water prevention and control during construction, as well as water leakage and discharge control during operation. The achievements of our research were applied to two large cross-section subsea tunnels in China, establishing a technical mode for safe construction under complex conditions.
TUNNELLING AND UNDERGROUND SPACE TECHNOLOGY
(2023)
Article
Cell Biology
Masaki Yamazaki, Atsuhiko Kato, Yoko Zaitsu, Takeshi Watanabe, Makoto Iimori, Shinichi Funahashi, Hiroyuki Kitao, Hiroshi Saeki, Eiji Oki, Masami Suzuki
ACTA HISTOCHEMICA ET CYTOCHEMICA
(2015)
Article
Biochemistry & Molecular Biology
Shin-Ichi Funahashi, Yasunori Suzuki, Kiyotaka Nakano, Shigeto Kawai, Masami Suzuki
JOURNAL OF BIOCHEMISTRY
(2017)
Article
Pathology
Masaki Yamazaki, Nami Yabuki, Yasunori Suzuki, Mayumi Ito, Asuka Ikeda, Osamu Natori, Masami Suzuki, Atsuhiko Kato
JOURNAL OF TOXICOLOGIC PATHOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Shin-Ichi Funahashi, Shigeto Kawai, Etsuko Fujii, Kenji Taniguchi, Kiyotaka Nakano, Shumpei Ishikawa, Hiroyuki Aburatani, Masami Suzuki
JOURNAL OF BIOCHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Takeshi Harada, Shuji Ozaki, Asuka Oda, Daisuke Tsuji, Akishige Ikegame, Masami Iwasa, Kengo Udaka, Shiro Fujii, Shingen Nakamura, Hirokazu Miki, Kumiko Kagawa, Yoshiaki Kuroda, Shigeto Kawai, Kohji Itoh, Hisafumi Yamada-Okabe, Toshio Matsumoto, Masahiro Abe
Article
Multidisciplinary Sciences
Shigeto Kawai, Masaki Yamazaki, Keita Shibuya, Masaya Yamazaki, Etsuko Fujii, Kiyotaka Nakano, Masami Suzuki
SCIENTIFIC REPORTS
(2020)
Article
Medicine, Research & Experimental
Masaki Yamazaki, Atsuhiko Kato, Eiji Oki, Yoko Zaitsu, Chie Kato, Kiyotaka Nakano, Miho Nakamura, Takuya Sakomura, Shigeto Kawai, Etsuko Fujii, Noriaki Sawada, Takeshi Watanabe, Hiroshi Saeki, Masami Suzuki
Summary: This study reveals that small clusters of tumor cells with high expression of LGR5 continuously form in the invasive front in a colorectal cancer xenograft model. These clusters are characterized by stress response and partial/hybrid epithelial-mesenchymal transition. They are important contributors to tumor growth and the expansion of cancer stem cells.
LABORATORY INVESTIGATION
(2021)
Article
Pathology
Minto Nakagawa, Shuji Hayashi, Saori Matsuo, Masaki Yamazaki, Atsuhiko Kato
Summary: This report describes the first case of lymph node lipomatosis in a young adult cynomolgus monkey with bilateral axillary lymph nodes. Macroscopic and histopathological examination revealed the replacement of lymphatic parenchyma by adipose tissue without any malignant findings.
JOURNAL OF TOXICOLOGIC PATHOLOGY
(2022)
Article
Cell Biology
Atsuko Murai, Masaki Yamazaki, Kaori Nishihara, Aki Kito, Sohei Oyama, Naoshi Horiba, Atsuhiko Kato
Summary: The article examined the pathological features of kidneys in a mouse model of SLE induced by a toll-like receptor 7 agonist, imiquimod (IMQ). The results showed that the mice exhibited pathological characteristics corresponding to class II lupus nephritis and podocyte injury.
HISTOLOGY AND HISTOPATHOLOGY
(2022)
Article
Cell Biology
Masaki Yamazaki, Atsuhiko Kato, Noriaki Sawada, Takeshi Watanabe, Masami Suzuki
Summary: Recurrence after chemotherapy is a major obstacle in cancer therapy; more detailed histopathological analyses are needed to understand tumor growth process; this study found that protruding structures on flattened ducts are a crucial first step in tumor regrowth.
HISTOLOGY AND HISTOPATHOLOGY
(2022)
Article
Pathology
Masaki Yamazaki, Etsuko Fujii, Takeshi Watanabe, Atsuhiko Kato, Masami Suzuki
JOURNAL OF TOXICOLOGIC PATHOLOGY
(2020)