Article
Virology
Hao Zheng, Yong Pan, Xiong Wang, Weibin Tian, Lunguang Yao, Jingchen Sun
Summary: By screening for suitable promoters, utilizing the envelope display effect of INVASIN and VSVG proteins, and constructing a recombinant baculovirus vector, the study was able to improve the efficiency and effectiveness of the baculovirus display system, providing a foundation for further development.
Review
Biotechnology & Applied Microbiology
Ezio Ricca, Loredana Baccigalupi, Rachele Isticato
Summary: Surface display systems have been developed to express target molecules on various biological entities and synthetic particles for technological and biomedical applications. The non-recombinant approach to display antigens and enzymes on bacterial spores shows potential applications in various fields.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Fisheries
Min Zhu, Zeen Shen, Yuchao Gu, Xinyu Tong, Yaxin Zhang, Jun Pan, Yongjie Feng, Xiaolong Hu, Yujun Wang, Guangli Cao, Renyu Xue, Chengliang Gong
Summary: This study developed a live-vector vaccine based on baculovirus technology to prevent diseases caused by highly pathogenic ISKNV infection. The vaccine successfully delivered and expressed the major capsid protein gene in fish cells and tissues, inducing expression of immune-related genes. ELISA results showed the production of specific antibodies in vaccinated fish. Injection and immersion vaccination trials demonstrated good immune efficacy in fish of different sizes.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Kai Hao, Yu Wang, Bin Zhu, Fei Yu, Zhe Zhao, Gao-Xue Wang
Summary: This study demonstrates that surface display of GCRV core antigen vaccine and invasion component from aquatic pathogenic microorganism on nonpathogenic Escherichia coli can effectively prevent GCRV viral diseases via the immersion administration approach.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Microbiology
Gang Zhang, Pu Wang, Lingling Jiang, Sheng Wang, Sinong Zhang, Yong Li
Summary: In this study, three vaccine candidates encoding the CCHFV glycoprotein Gn and nucleocapsid protein (Np) were prepared using an insect baculovirus vector expression system. The results showed that the recombinant baculoviruses were able to express both Gn and Np and induce significant humoral immunity in mice. However, coexpression of Gn and Np did not improve immunogenicity, suggesting that the recombinant baculovirus displaying Gn alone has potential as a CCHF vaccine candidate. This study provides new insights for the development of a CCHF baculovirus vaccine.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Esma Mouhoub, Pilar Domenech, Momar Ndao, Michael B. Reed
Summary: Live attenuated Bacillus Calmette-Guerin (BCG) vaccine is widely used for preventing tuberculosis, but has also shown protection against other pathogens. Recombinant BCG technology has been used to create vaccines that elicit immunity against bacterial, viral, and parasitic diseases. This review aims to provide an overview of rBCG-based vaccines targeting infectious diseases other than TB.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ying Sun, Yongrong Zhang, Hua Yu, Ashley Saint Fleur, Di Yu, Zhiyong Yang, Hanping Feng
Summary: In this proof-of-concept study, a versatile platform was developed using Saccharomyces cerevisiae to rapidly identify antibodies with both high affinity and neutralizing activity. The platform successfully expressed engineered chimeric-bispecific Fab antibodies and enabled the construction and enrichment of a combinatorial Fab library. The screening process led to the identification of two unique neutralizers that can neutralize the cytotoxicity of TcdB.
MICROBIAL CELL FACTORIES
(2023)
Review
Oncology
Miaojin Zhu, Jia Ji, Danrong Shi, Xiangyun Lu, Baohong Wang, Nanping Wu, Jie Wu, Hangping Yao, Lanjuan Li
Summary: Monkeypox, caused by the monkeypox virus, has become a global concern during the ongoing COVID-19 pandemic. The re-emergence of this virus and outbreaks in multiple nonendemic countries require attention and control measures.
FRONTIERS OF MEDICINE
(2022)
Article
Immunology
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Jie Mao, Su-Hwa Lee, Md Atique Ahmed, Fu-Shi Quan
Summary: This study found that oral immunization with Toxoplasma gondii vaccines induced a similarly effective immune response as intranasal or intramuscular immunizations, providing a protective effect against lethal infections in mice.
Article
Immunology
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Su-Hwa Lee, Eun-Kyung Moon, Fu-Shi Quan
Summary: Intranasal immunization with T. gondii ROP4 rBVs induces better mucosal and systemic immunity, leading to enhanced protection against lethal challenge infection compared to oral immunization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biology
Tingting Li, Ciying Qian, Ying Gu, Jun Zhang, Shaowei Li, Ningshao Xia
Summary: This article provides an overview of recent advances in the development of both conventional prophylactic vaccines and therapeutic vaccines. It covers different vaccine forms and their applications in various diseases. The aim is to offer new insights and technologies for future vaccine design.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Oncology
Ebrahim Kord, Farzin Roohvand, Jean Dubuisson, Thibaut Vausselin, Hosein Nasr Azadani, Abolfazl Keshavarz, Ahmad Nejati, Katayoun Samimi-Rad
Summary: The study demonstrated a BacMam virus-based surface display for subtype-1a HCV gpE2, showing proper expression and incorporation, and successful induction of strong humoral and cellular immune responses in immunized mice. The BacMam virus-based surface display for gpE2 offers a promising strategy for the development of an efficient HCV vaccine.
INFECTIOUS AGENTS AND CANCER
(2021)
Review
Biochemistry & Molecular Biology
Layaly Shkair, Ekaterina E. Garanina, Robert J. Stott, Toshana L. Foster, Albert A. Rizvanov, Svetlana F. Khaiboullina
Summary: The prevention and control of infectious diseases is crucial, with vaccination being an effective approach. Microvesicles are suggested as potential vehicles for antigen delivery, carrying various immunogenic molecules directly to target cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Yixin Wang, Ting-Jing Chen-Mayfield, Zhaoting Li, Muhsin H. Younis, Weibo Cai, Quanyin Hu
Summary: Despite the rapid development of immunotherapy, low response rates, poor therapeutic outcomes, and severe side effects still limit its implementation. DNA has recently emerged as an important player in immune modulation. Researchers have utilized DNA or nucleic acid-based immune stimulators for the prevention and treatment of diseases. Additionally, DNA delivery vehicles and nanostructures have been used to regulate immune systems and elicit strong immune responses.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Immunology
Chih-Chi Liao, Chih-Hsuan Tsai, Huei-Ru Lo, Pey-Ru Lin, Chang-Chi Lin, Yu-Chan Chao
Summary: Scrub typhus is a fatal epidemic in the Asia-Pacific region, often misdiagnosed, and the lack of accurate detection methods poses a global health threat. Researchers successfully expressed O. tsutsugamushi antigens on insect cell surfaces and developed a cell-based ELISA detection method with high accuracy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Subaschandrabose Rajesh Kumar, Mookkan Prabakaran, Kattur Venkatachalam Ashok Raj, Fang He, Jimmy Kwang
Review
Virology
Shawn Zheng Kai Tan, Mark Zheng Yi Tan, Mookkan Prabakaran
REVIEWS IN MEDICAL VIROLOGY
(2017)
Article
Virology
Shawn Zheng Kai Tan, Mookkan Prabakaran
Article
Virology
Shawn Zheng Kai Tan, Kaw Bing Chua, Yishi Xu, Mookkan Prabakaran
Article
Immunology
Yishi Xu, Carla Bianca Luena Victorio, Qimei Ng, Mookkan Prabakaran, Yee-Joo Tan, Kaw Bing Chua
EMERGING MICROBES & INFECTIONS
(2016)
Article
Virology
Subaschandrabose Rajesh Kumar, Sharenya Chelvaretnam, Yunrui Tan, Mookkan Prabakaran
Article
Multidisciplinary Sciences
Mookkan Prabakaran, Sonja Leyrer, Fang He, Sebastian Auer, Subaschandrabose R. Kumar, Kathrin Kindsmueller, Nutan Mytle, Joerg Schneider, Stephen Lockhart, Jimmy Kwang
Article
Chemistry, Analytical
Manchugondanahalli S. Krishna, Desiree-Faye Kaixin Toh, Zhenyu Meng, Alan Ann Lerk Ong, Zhenzhang Wang, Yunpeng Lu, Kelin Xia, Mookkan Prabakaran, Gang Chen
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Julita Kesy, Kiran M. Patil, Subaschandrabose Rajesh Kumar, Zhiyu Shu, Hui Yee Yong, Louis Zimmermann, Alan Ann Lerk Ong, Desiree-Faye Kaixin Toh, Manchugondanahalli S. Krishna, Lixia Yang, Jean-Luc Decout, Dahai Luo, Mookkan Prabakaran, Gang Chen, Elzbieta Kierzek
BIOCONJUGATE CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Manchugondanahalli S. Krishna, Zhenzhang Wang, Liangzhen Zheng, Jogesh Bowry, Alan Ann Lerk Ong, Yuguang Mu, Mookkan Prabakaran, Gang Chen
Article
Immunology
Zhong Wee Poh, Zhenzhang Wang, Subaschandrabose Rajesh Kumar, Hui Yee Yong, Mookkan Prabakaran
Article
Biochemical Research Methods
Huan Jia, Eric A. Miller, Chia Ching Chan, Say Yong Ng, Mookkan Prabakaran, Meng Tao, Ian Shen-Yi Cheong, Sing Mei Lim, Ming Wei Chen, Xiaohong Gao, R. Abirami, Megan E. McBee, Peter R. Preiser, Hadley D. Sikes, Patthara Kongsuphol
Summary: Surveillance of SARS-CoV-2 infection is crucial for pandemic control. The availability of antigen rapid tests (ARTs) is limited due to the shortage of nitrocellulose paper. Vertical flow assay (VFA) with cellulose paper as a test material is considered as a complementary approach. However, current VFAs face challenges in reading test signals from the bottom face of the test cassette, hindering their application as rapid tests.
Article
Virology
Tasnim Saifudin Zakir, Tao Meng, Lee Ching Pei Carmen, Justin Jang Hann Chu, Raymond Tzer Pin Lin, Mookkan Prabakaran
Summary: A broadly neutralizing monoclonal antibody 9G8 has been found to show potent neutralization against multiple variants of SARS-CoV-2, and two mutations within the receptor-binding domain (RBD) have been identified to be involved in the virus's evasion from antibody 9G8.
Review
Virology
Rajamanonmani Ravikumar, Janlin Chan, Mookkan Prabakaran
Summary: This article discusses the issue of viral infections in the poultry industry and the strategies for vaccination. The poultry industry faces economic losses and impacts on the global food supply chain due to viral outbreaks. The development of broadly protective vaccines is crucial for alleviating vaccine selection pressure and saving husbandry costs.