期刊
WATER RESEARCH
卷 141, 期 -, 页码 185-195出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2018.05.002
关键词
Partial saturation; Nitrifying-denitrifying prokaryotes; Nitrification-aggregates; Metabolically active microbiome; Nitrogen metabolism
资金
- Spanish Ministry of Economy and Competitiveness [FJCI-2014-22245]
- Coordination for the Improvement of Higher Level (CAPES) [PDSE 99999.006475/2015-09]
- CERCA Programme/Generalitat de Catalunya
Nitrogen dynamics and its association to metabolically active microbial populations were assessed in two vertical subsurface vertical flow (VF) wetlands treating urban wastewater. These VF wetlands were operated in parallel with unsaturated (UVF) and partially saturated (SVF) configurations. The SVF wetland exhibited almost 2-fold higher total nitrogen removal rate (5g TN m(-2) d(-1)) in relation to the UVF wetland (3g TN m(-2) d(-1)), as well as a low NOx-N accumulation (1mg L-1 vs. 26 mg L-1 in SVF and UVF wetland effluents, respectively). After 6 months of operation, ammonia oxidizing prokaryotes (AOP) and nitrite oxidizing bacteria (NOB) displayed an important role in both wetlands. Oxygen availability and ammonia limiting conditions promoted shifts on the metabolically active nitrifying community within 'nitrification aggregates' of wetland biofllms. Ammonia oxidizing archaea (AOA) and Nitrospira spp. overcame ammonia oxidizing bacteria (AOB) in the oxic layers of both wetlands. Microbial quantitative and diversity assessments revealed a positive correlation between Nitrobacter and AOA, whereas Nitrospira resulted negatively correlated with Nitrobacter and AOB populations. The denitrifying gene expression was enhanced mainly in the bottom layer of the SVF wetland, in concomitance with the depletion of NOx-N from wastewater. Functional gene expression of nitrifying and denitrifying populations combined with the active microbiome diversity brought new insights on the microbial nitrogen cycling occurring within VF wetland biofllms under different operational conditions. (C) 2018 Elsevier Ltd. All rights reserved.
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