Article
Microbiology
Yuan Gao, Zheng Wang, Jiani Fu, Jiachang Cai, Tengfei Ma, Ning Xie, Run Fan, Weishuai Zhai, Andrea T. Fessler, Chengtao Sun, Congming Wu, Stefan Schwarz, Rong Zhang, Yang Wang
Summary: The presence and transmission of the multidrug resistance gene cfr in staphylococci from humans and animals were investigated. The study found that the cfr gene was detected in human clinical isolates, with Staphylococcus capitis being the predominant species. The cfr-carrying plasmid pLRSA417 was identified and found to be present in staphylococci from multiple geographic regions and different hosts, suggesting possible transmission between humans and animals.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Biao Tang, Juan Ni, Jiahui Lin, Yangying Sun, Hui Lin, Yuehong Wu, Hua Yang, Min Yue
Summary: This study screened eight multi-drug resistant E. coli strains carrying the cfr gene from a collection of E. coli strains isolated from pigs and chickens in China in 2021. The study confirmed the transfer mechanism of the cfr gene through conjugation and identified two plasmids harboring the cfr gene. Additionally, the study demonstrated the formation of cfr-IS26 circular intermediates in some E. coli strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Dong Wang, Liang-Xing Fang, Yu-Wei Jiang, De-Shu Wu, Qi Jiang, Ruan-Yang Sun, Min-Ge Wang, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao
Summary: This study investigated the prevalence and molecular characteristics of fosA3 and fosA7 among Salmonella isolates. The results showed that fosA7 and fosA3 exhibited different levels of resistance and distribution patterns in different strains. Additionally, fosA7 was found to enhance Salmonella survival under stress conditions. This study is important for understanding the mechanisms and molecular characteristics of fosfomycin resistance in Salmonella.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Steven Dunn, Laura Carrilero, Michael Brockhurst, Alan McNally
Summary: The study found that different E. coli strains vary in their ability to acquire and maintain MDR plasmids, with highly strain-specific transcriptional responses observed following plasmid acquisition. However, the subtle transcriptional responses consistent across all strains suggest that fitness costs arising from transcriptional disruption are unlikely to act as a barrier to dissemination of this MDR plasmid in E. coli.
Article
Infectious Diseases
Nunziatina Russo, Alessandro Stamilla, Giuseppe Cascone, Cinzia Lucia Randazzo, Antonino Messina, Massimiliano Lanza, Alessandra Pino, Cinzia Caggia, Francesco Antoci
Summary: This study focused on the biodiversity, pathogenicity, and antibiotic resistance profile of 104 E. coli strains isolated from domestic animals in Eastern Sicily. The strains showed multi-resistance against at least three antibiotic classes, with high levels of resistance observed against amoxicillin, tylosin, sulfamethoxazole, and erythromycin. The study also identified two EPEC strains and highlighted a wide variety of genotyping profiles through PFGE analysis.
Article
Biochemistry & Molecular Biology
Manuel Ares-Arroyo, Charles Coluzzi, Eduardo P.C. Rocha
Summary: Plasmids can be transferred between cells by conjugation, driving bacterial evolution. By studying plasmids in Escherichia coli and Staphylococcus aureus, we have solved the mystery of plasmid transfer mechanisms and revealed the functional dependencies between plasmids. These findings are important for understanding the evolutionary relationships of plasmids and the spread of antibiotic resistance genes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Infectious Diseases
Yujie Hu, Seamus Fanning, Scott Nguyen, Wei Wang, Chang Liu, Xinnan Cui, Yinping Dong, Xin Gan, Jin Xu, Fengqin Li
Summary: CFSA629, a MDR S. Typhimurium ST34 isolate from China, carries a novel mcr-1.19 variant on an IncHI2 plasmid, highlighting the importance of surveillance to prevent the spread of mcr genes among foodborne Salmonella. Improved surveillance is crucial in controlling the dissemination of mcr genes worldwide.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Yan Li, Panpan Wang, Xia Xiao, Ruichao Li, Zhiqiang Wang
Summary: This study investigated the prevalence of tet(X4) in slaughterhouses and found that it was widespread and dominated by E. coli. Genome analysis revealed the transmission of tet(X4) was mainly mediated by plasmids, and an IncX1 plasmid carrying more resistance genes was identified. The findings suggest that slaughterhouses are important sources and transmission hotspots of tet(X4), highlighting the need for large-scale surveillance and interventions to prevent its transmission.
VETERINARY MICROBIOLOGY
(2023)
Article
Food Science & Technology
Feng Zhang, Shi Wu, Tao Lei, Qingping Wu, Jumei Zhang, Jiahui Huang, Jingsha Dai, Moutong Chen, Yu Ding, Juan Wang, Xianhu Wei, Youxiong Zhang
Summary: Staphylococcus aureus strain 2868B2 isolated from frozen dumplings in Hangzhou in 2015 carried multiple drug resistance genes, showing resistance to more than 18 antibiotics with high-level resistance to several. The cfr and lsa(E) genes were found on separate plasmids with the presence of IS elements facilitating gene mobility.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Infectious Diseases
Yanyu Pan, Bin Hu, Xiangning Bai, Xi Yang, Lijiao Cao, Qian Liu, Hui Sun, Juan Li, Ji Zhang, Dong Jin, Yanwen Xiong
Summary: This study investigated the antimicrobial resistance of non-O157 Shiga toxin-producing Escherichia coli (STEC) strains in China, revealing diverse resistance patterns and the presence of various resistance genes among strains from different sources. Whole genome analysis showed genetic diversity among STEC strains from different sources, with some strains tending to cluster closely within the phylogenetic tree. These findings provide valuable information for monitoring and guiding the use of antimicrobial drugs in the management of multidrug resistant STEC strains among animals and humans.
Article
Infectious Diseases
Mitja N. P. Remus-Emsermann, David Aicher, Cosima Pelludat, Pascal Gisler, David Drissner
Summary: The study suggests that bacteria in the phyllosphere may play a role in transferring antibiotic resistance genes to human pathogenic bacteria. Results indicate that the short-term presence of a competitive donor is sufficient to fix plasmids in the recipient population, but the spread of self-transmissible plasmids is limited. Understanding plasmid transfer data from this study will be crucial for modeling the environmental spread of antibiotic resistance in agricultural production environments in the future.
Article
Microbiology
Julian A. Paganini, Nienke L. Plantinga, Sergio Arredondo-Alonso, Rob J. L. Willems, Anita C. Schurch
Summary: Infections caused by multidrug-resistant E. coli strains have increased in recent years. A study of 25 available plasmid prediction tools found that MOB-suite was the most effective in correctly reconstructing the majority of plasmids.
Article
Microbiology
Adam Valcek, Petra Sismova, Kristina Nesporova, Soren Overballe-Petersen, Ibrahim Bitar, Ivana Jamborova, Arie Kant, Jaroslav Hrabak, Jaap A. Wagenaar, Jean-Yves Madec, Peter Damborg, Engeline van Duijkeren, Christa Ewers, Joost Hordijk, Henrik Hasman, Michael S. M. Brouwer, Monika Dolejska
Summary: The study found that the equine-associated Escherichia coli strains ST1250 and its variants ST1250-SLV/DLV exhibit high genetic diversity at the genomic level, with a portion of the samples carrying the epidemic multidrug resistance plasmid lineage IncHI1/ST9 and fos operon associated with E. coli-ST1250.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Ethan R. Wyrsch, Rhys N. Bushell, Marc S. Marenda, Glenn F. Browning, Steven P. Djordjevic
Summary: Lower urinary tract, renal, and bloodstream infections caused by phylogroup B2 extraintestinal pathogenic Escherichia coli (ExPEC) are a leading cause of morbidity and mortality. ST1193 is a multidrug-resistant sequence type of B2 phylogroup E. coli that is associated with various human infections. This study analyzed the virulence plasmids carried by ST1193 and found that they are similar to pUTI89, a key F virulence plasmid found in dominant extraintestinal pathogenic E. coli lineages. The findings of this study have significant implications for the epidemiological source tracking of ExPEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jing Wang, Meng-Jun Lu, Zhen-Yu Wang, Yue Jiang, Han Wu, Zhi-Ming Pan, Xinan Jiao
Summary: This study investigated the prevalence and characterization of tet(X4) in Escherichia coli isolates from a pig farm in Shanghai, China, and revealed the dissemination mechanism of tet(X4) in this swine farm. The results showed clonal transmission of tet(X4)-positive E. coli ST761 in this pig farm.
FRONTIERS IN MICROBIOLOGY
(2022)
Letter
Microbiology
Yao Zhu, Lei Liu, Stefan Schwarz, Wenyu Liu, Changzhen Wang, Qin Yang, Tian Luan, Lingli Wang, Siguo Liu, Wanjiang Zhang
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Letter
Infectious Diseases
Yao Zhu, Wanjiang Zhang, Changzhen Wang, Wenyu Liu, Qin Yang, Tian Luan, Lingli Wang, Stefan Schwarz, Siguo Liu
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Review
Microbiology
Stefan Schwarz, Wanjiang Zhang, Xiang-Dang Du, Henrike Krueger, Andrea T. Fessler, Shizhen Ma, Yao Zhu, Congming Wu, Jianzhong Shen, Yang Wang
Summary: Mobile oxazolidinone resistance genes such as cfr, optrA, and poxtA are found on various platforms and may overlap with other resistance genes, contributing to the dissemination of resistance among microbial populations.
CLINICAL MICROBIOLOGY REVIEWS
(2021)
Letter
Infectious Diseases
Yao Zhu, Qin Yang, Stefan Schwarz, Wenlin Yang, Qiu Xu, Lingli Wang, Siguo Liu, Wanjiang Zhang
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Qin Yang, Yao Zhu, Stefan Schwarz, Lingli Wang, Wenyu Liu, Wenlin Yang, Tian Luan, Siguo Liu, Wanjiang Zhang
Summary: Tetracyclines are broad-spectrum antibiotics used in veterinary medicine and food animal production. This study identified tandem tetracycline resistance genes tet(61)-tet(58) on the novel plasmid pT4303, which can confer resistance to both Gram-positive and Gram-negative bacteria. The presence of multiple tet genes on the same plasmid suggests a potential vehicle for the dissemination of tetracycline resistance genes.
VETERINARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Yao Zhu, Wenlin Yang, Stefan Schwarz, Qiu Xu, Qin Yang, Lingli Wang, Siguo Liu, Wanjiang Zhang
Summary: This study investigated the genetic context and transferability of oxazolidinone resistance genes in a porcine Vagococcus lutrae isolate. The researchers found that the isolate carried the cfr(D) and optrA resistance genes, which were located on a novel pseudo-compound transposon and a broad-host-range plasmid, respectively. The study highlighted the potential risk of transfer of these resistance genes from V. lutrae to clinically more important bacterial pathogens.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Letter
Infectious Diseases
Yao Zhu, Wenyu Liu, Stefan Schwarz, Lei Liu, Wenlin Yang, Qin Yang, Lingli Wang, Tian Luan, Siguo Liu, Wanjiang Zhang
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Yao Zhu, Wenlin Yang, Stefan Schwarz, Qiu Xu, Qin Yang, Lingli Wang, Siguo Liu, Wanjiang Zhang
Summary: This study reports the first discovery of the transferable oxazolidinone resistance gene poxtA in the genus Lactobacillus and investigates its molecular characteristics and horizontal transferability. Additionally, the presence of the poxtA gene on both plasmid and chromosomal DNA, as well as the formation of translocatable units carrying resistance genes, was analyzed.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Wenlin Yang, Qiu Xu, Qin Yang, Lingli Wang, Siguo Liu, Yao Zhu, Wanjiang Zhang
Summary: The occurrence and dissemination of linezolid-resistant Gram-positive bacteria among food-producing animals poses severe threats to public health. In this study, the optrA resistance gene was found in a multi-drug resistant strain of E.faecalis isolated from a goat in China. The strain belonged to the high-risk clone ST16 and carried the optrA gene on a novel plasmid. Active surveillance of optrA-positive E.faecalis high-risk clones in food-producing animals is urgently warranted.
VETERINARY MICROBIOLOGY
(2022)
Article
Infectious Diseases
Qin Yang, Yao Zhu, Stefan Schwarz, Lingli Wang, Wenyu Liu, Wenlin Yang, Siguo Liu, Wanjiang Zhang
Summary: This study identified a novel integrative and conjugative element (ICE) that can mobilize resistance plasmids and translocatable units (TUs), contributing to the spread of antibiotic resistance. The formation of different cointegrates between ICEs, plasmids, and TUs was confirmed through PCR assays. Conjugation assays showed that ICEs can transfer non-conjugative MGEs, such as TUs and plasmids, into recipient strains.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Yao Zhu, Qiu Xu, Stefan Schwarz, Shenye Yu, Lining Xia, Fang Xie, Wenlin Yang, Longyu Zhou, Longhua Lin, Wanjiang Zhang
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Huihui Zhang, Xinyuan Zhang, Siyu Liang, Jing Wang, Yao Zhu, Wanjiang Zhang, Siguo Liu, Stefan Schwarz, Fang Xie
Summary: The combination of phage endolysin Ply2660 and antimicrobial peptide LL-37 showed superior bactericidal efficacy against drug-resistant biofilm-producing E. faecalis. The combination also suppressed biofilm formation and exhibited enhanced biofilm killing compared to individual treatments. In a murine peritoneal septicemia model, the Ply2660-LL-37 combination effectively suppressed E. faecalis dissemination and attenuated organ injury.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Infectious Diseases
Qin Yang, Yao Zhu, Stefan Schwarz, Wanjiang Zhang, Xiumei Wang
Summary: In this study, a novel genetic element involved in the horizontal transfer of the oxazolidinone/phenicol resistance gene optrA was identified in Streptococcus suis. A novel circularizable structure (UCS) and a novel integrative and conjugative element (ICE) containing the optrA gene were found. Conjugation assays confirmed the transferability of ICESsuHN38 to the recipient strain.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Public, Environmental & Occupational Health
Lichao Ma, Wanjiang Zhang, Jingyun Ma, Qingpo Cui, Chaoyang Zhang, Peng Zhang, Chengtao Sun, Huarun Sun, Yao Zhu, Shaolin Wang, Shuangyang Ding, Gongzheng Hu, Zhangqi Shen
Summary: The study found that the prevalence of antimicrobial resistance genes in pig isolates of S. suis was higher than in human isolates, and the prevalence of these genes also varied with serotype. These data will help guide rational drug use in the clinic to better protect the health of humans and animals.
Letter
Infectious Diseases
Yao Zhu, Wanjiang Zhang, Siguo Liu, Stefan Schwarz
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)