Review
Immunology
Beth-Ann G. Coller, William Lapps, Mahum Yunus, Samantha Bruno, Michael J. Eichberg, Andrew Wen-Tseng Lee, Kenneth Liu, Rosybel Drury, Jules Millogo, Louis Robert Macareo, Thomas H. Armstrong, Jeffrey T. Blue, Lynne A. Isopi, Melissa Hughes, Susan M. VanRheenen, Jonathan Deutsch, Joan G. Tell, Sheri A. Dubey
Summary: This review discusses the development of the rVSV Delta G-ZEBOV-GP Ebola vaccine and the efforts made to overcome challenges and expand knowledge of the product. The vaccine has been approved by various organizations and countries, making it the first registered Ebola vaccine stockpile available for outbreak response. Additionally, the development of this vaccine provides insights for the development of vaccines for other emerging epidemic diseases.
Editorial Material
Biochemistry & Molecular Biology
Luca Zinzula
Summary: The authors present a structure of VP40 from Sudan ebolavirus, which contains a previously unresolved disulfide bridge that allows regulation of VP40 functions via human thio-redoxin.
Article
Virology
Palaniappan Ramanathan, Bersabeh Tigabu, Rodrigo I. Santos, Philipp A. Ilinykh, Natalia Kuzmina, Olivia A. Vogel, Naveen Thakur, Hamza Ahmed, Chao Wu, Gaya K. Amarasinghe, Christopher F. Basler, Alexander Bukreyev
Summary: Members of the Ebolavirus genus exhibit varying levels of pathogenicity in humans, with Ebola (EBOV) being the most pathogenic, Bundibugyo (BDBV) less pathogenic, and Reston (RESTV) not known to cause disease. The VP24 protein encoded by these viruses plays a role in blocking the immune response, contributing to virulence. By studying the interaction between VP24 and host proteins, researchers found that modifying the interface between EBOV VP24 and host karyopherin alpha can attenuate its ability to antagonize the immune response. This study highlights the importance of VP24 in the pathogenicity of Ebolaviruses.
Article
Microbiology
Adam J. Hume, Baylee Heiden, Judith Olejnik, Ellen L. Suder, Stephen Ross, Whitney A. Scoon, Esther Bullitt, Maria Ericsson, Mitchell R. White, Jacquelyn Turcinovic, Tran T. N. Thao, Ryan M. Hekman, Joseph E. Kaserman, Jessie Huang, Konstantinos-Dionysios Alysandratos, Gabor E. Toth, Ferenc Jakab, Darrell N. Kotton, Andrew A. Wilson, Andrew Emili, Volker Thiel, John H. Connor, Gabor Kemenesi, Daniel Cifuentes, Elke Muhlberger
Summary: Next generation sequencing has helped identify and study RNA viruses in animal hosts that have the potential to infect humans. In this study, researchers successfully used a minigenome system to rescue and study an uncultured filovirus called Lloviu virus (LLOV), which is closely related to Ebola virus. They were able to generate recombinant LLOV and study its replication, infection, and response to antiviral drugs. This research provides valuable insights into the pathogenic potential of LLOV and could aid in pandemic preparedness.
Article
Pharmacology & Pharmacy
Shuzo Urata, Olaposi Idowu Omotuyi, Ayako Izumisawa, Takeshi Ishikawa, Satoshi Mizuta, Yasuteru Sakurai, Tatsuaki Mizutani, Hiroshi Ueda, Yoshimasa Tanaka, Jiro Yasuda
Summary: This study reveals the dimerization mechanism of VP40 and identifies two compounds through computational screening and in vitro assays, which may serve as lead drug candidates against Filovirus diseases.
ANTIVIRAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Mathavan Muthaiyan, Leimarembi Devi Naorem, Vassavi Seenappa, Shilpa Sri Pushan, Amouda Venkatesan
Summary: Ebola virus is a deadly pathogen causing hemorrhagic fever, and a study was conducted to analyze the interactions between 270 human proteins and EBOV, creating protein-protein interaction networks to identify potential drug targets against Ebola. Gene enrichment and pathway analysis provided insight into the importance of EBOV-human interactions, with the inclusion of a human-based drug targeted therapy highlighted as significant in drug repurposing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Gastroenterology & Hepatology
Nabeel Khan, Chinmay Trivedi, Faten Aberra, Tyler Pernes, Yu-Xiao Yang
Summary: In a retrospective cohort study using data from the Veterans Affairs Healthcare System, it was found that administration of recombinant zoster vaccine was not associated with an increased risk of inflammatory bowel disease flare within 90 days.
JOURNAL OF CROHNS & COLITIS
(2022)
Article
Medicine, General & Internal
Supriya Ravichandran, Surender Khurana
Summary: A serodiagnostic test has been developed to differentiate between individuals with EBOV infection-induced antibodies and those with EBOV vaccine-induced antibodies. This test can accurately detect Ebola virus infections and could be implemented as a robust diagnostic tool for epidemiology and surveillance during and after outbreaks, especially in countries with mass Ebola vaccinations.
Article
Immunology
Felix B. He, Hira Khan, Moona Huttunen, Pekka Kolehmainen, Krister Melen, Sari Maljanen, Mengmeng Qu, Miao Jiang, Laura Kakkola, Ilkka Julkunen
Summary: Filovirus family is a highly pathogenic virus family that has caused deadly outbreaks, especially in African countries. Previous research has focused on Ebola, Sudan, and Marburg viruses, leaving other filoviruses less studied. These viruses have the ability to downregulate the innate immune system, and VP24 proteins have been shown to interfere with interferon gene expression and signaling.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
John Misasi, Nancy J. Sullivan
Summary: The article reviews the treatment methods of Ebola virus outbreak, including two therapies approved by the FDA using monoclonal antibodies. It introduces a new method to define vulnerable sites on the viral glycoprotein, hoping to combat Ebola virus and other emerging viruses.
Article
Immunology
Amina Basheer, Syed Babar Jamal, Badr Alzahrani, Muhammad Faheem
Summary: Dengue fever is a mosquito-borne disease that affects approximately 3.9 billion people globally each year. Currently, there is no available vaccine or drug for dengue virus infection. In this study, researchers designed a tetravalent subunit multi-epitope vaccine targeting different proteins from each serotype. The vaccine has shown potential to elicit a strong immune response against all dengue serotypes without causing any adverse effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Rahul Kumar, Robert E. Porter, Sunil K. Mor, Sagar M. Goyal
Summary: A recombinant live virus-vectored bivalent codon optimized subunit vaccine was created to express immunogenic proteins of turkey arthritis reovirus. The vaccine virus could be transmitted horizontally to immunize others. The vaccine showed efficacy against both homologous and heterologous virus challenge and produced neutralizing antibodies. It also did not have adverse effects on feed efficiency. The replication of challenge viruses was decreased in vaccinated birds' intestines and tendons.
Article
Infectious Diseases
Angela Huttner, Selidji Todagbe Agnandji, Olivier Engler, Jay W. Hooper, Steve Kwilas, Keersten Ricks, Tamara L. Clements, Hulda R. Jonsdottir, Sravya Sowdamini Nakka, Sylvia Rothenberger, Peter Kremsner, Roland Zust, Donata Medaglini, Tom Ottenhoff, Ali M. Harandi, Claire-Anne Siegrist
Summary: This study reports the 5-year persistence and avidity of antibodies produced by the live-attenuated recombinant vesicular stomatitis virus expressing the Zaire Ebolavirus glycoprotein. The findings suggest that antibody titres at 1 year can predict antibody persistence at 5 years. Additionally, while antibody titres remained stable, the duration of live-virus neutralizing antibodies was shorter.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Biotechnology & Applied Microbiology
Ehud Shahar, Ken Emquies, Itai Bloch, Dalia Eliahu, Ran Ben Adiva, Jacob Pitcovski, Itamar Yadid
Summary: This study demonstrates the potential of LPS-free gram-negative bacteria as an important tool for protein expression, with wide applications in research and biotechnology.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anke-Dorothee Werner, Martin Schauflinger, Michael J. Norris, Michael Kluever, Anna Trodler, Astrid Herwig, Christina Brandstaedter, Melissa Dillenberger, Gerhard Klebe, Andreas Heine, Erica Ollmann Saphire, Katja Becker, Stephan Becker
Summary: This study reveals the importance of two cysteine residues in the flexible C-terminal arm of the Ebola virus matrix protein VP40. These cysteines form a stabilizing disulfide bridge and interact with the host's thioredoxin system. Mutation of these cysteines impairs the budding function of VP40 and relaxes its inhibitory role for viral RNA synthesis.
Article
Chemistry, Multidisciplinary
Polina Brangel, Ariel Sobarzo, Claudio Parolo, Benjamin S. Miller, Philip D. Howes, Sigal Gelkop, Julius J. Lutwarna, John M. Dye, Rachel A. McKendry, Leslie Lobel, Molly M. Stevens
Article
Virology
Gaya K. Amarasinghe, Nidia G. Arechiga Ceballos, Ashley C. Banyard, Christopher F. Basler, Sina Bavari, Andrew J. Bennett, Kim R. Blasdell, Thomas Briese, Alexander Bukreyev, Yingyun Cai, Charles H. Calisher, Cristine Campos Lawson, Kartik Chandran, Colin A. Chapman, Charles Y. Chiu, Kang-Seuk Choi, Peter L. Collins, Ralf G. Dietzgen, Valerian V. Dolja, Olga Dolnik, Leslie L. Domier, Ralf Duerrwald, John M. Dye, Andrew J. Easton, Hideki Ebihara, Juan E. Echevarria, Anthony R. Fooks, Pierre B. H. Formenty, Ron A. M. Fouchier, Conrad M. Freuling, Elodie Ghedin, Tony L. Goldberg, Roger Hewson, Masayuki Horie, Timothy H. Hyndman, Daohong Jiang, Robert Kityo, Gary P. Kobinger, Hideki Kondo, Eugene V. Koonin, Mart Krupovic, Gael Kurath, Robert A. Lamb, Benhur Lee, Eric M. Leroy, Piet Maes, Andrea Maisner, Denise A. Marston, Sunil Kumar Mor, Thomas Mueller, Elke Muhlberger, Victor Manuel Neira Ramirez, Sergey V. Netesov, Terry Fei Fan Ng, Norbert Nowotny, Gustavo Palacios, Jean L. Patterson, Janusz T. Paweska, Susan L. Payne, Karla Prieto, Bertus K. Rima, Paul Rota, Dennis Rubbenstroth, Martin Schwemmle, Stuart Siddell, Sophie J. Smither, Qisheng Song, Timothy Song, Mark D. Stenglein, David M. Stone, Ayato Takada, Robert B. Tesh, Luciano Matsumiya Thomazelli, Keizoi Tomonaga, Noel Tordo, Jonathan S. Towner, Nikos Vasilakis, Sonia Vazquez-Moron, Claudio Verdugo, Viktor E. Volchkov, Victoria Wahl, Peter J. Walker, David Wang, Lin-Fa Wang, James F. X. Wellehan, Michael R. Wiley, Anna E. Whitfield, Yuri I. Wolf, Gongyin Ye, Yong-Zhen Zhang, Jens H. Kuhn
ARCHIVES OF VIROLOGY
(2018)
Article
Biochemistry & Molecular Biology
Elisabeth K. Nyakatura, Samantha E. Zak, Anna Z. Wec, Daniel Hofmann, Sergey Shulenin, Russell R. Bakken, M. Javad Aman, Kartik Chandran, John M. Dye, Jonathan R. Lai
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Immunology
Thomas Luke, Richard S. Bennett, Dawn M. Gerhardt, Tracey Burdette, Elena Postnikova, Steven Mazur, Anna N. Honko, Nicholas Oberlander, Russell Byrum, Dan Ragland, Marisa St Claire, Krisztina B. Janosko, Gale Smith, Gregory Glenn, Jay Hooper, John Dye, Subhamoy Pal, Kimberly A. Bishop-Lilly, Theron Hamilton, Kenneth Frey, Laura Bollinger, Jiro Wada, Hua Wu, Jin-an Jiao, Gene G. Olinger, Bronwyn Gunn, Galit Alter, Surender Khurana, Lisa E. Hensley, Eddie Sullivan, Peter B. Jahrling
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Immunology
Jerry F. Brown, John M. Dye, Sam Tozay, Gertrude Jeh-Mulbah, David A. Wohl, William A. Fischer, Coleen K. Cunningham, Kathleen Rowe, Peter Zacharias, James van Hasselt, David A. Norwood, Nathan M. Thielman, Samantha E. Zak, David L. Hoover
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Chemistry, Medicinal
Szu-Yuan Pu, Randy Wouters, Stanford Schor, Jef Rozenski, Rina Barouch-Bentov, Laura Prugar, Cecilia M. O'Brien, Jennifer M. Brannan, John M. Dye, Piet Herdewijn, Steven De Jonghe, Shirit Einav
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Immunology
Avishay Edri, Avishai Shemesh, Muhammed Iraqi, Omri Matalon, Michael Brusilovsky, Uzi Hadad, Olga Radinsky, Orly Gershoni-Yahalom, John M. Dye, Ofer Mandelboim, Mira Barda-Saad, Leslie Lobel, Angel Porgador
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Immunology
Michelle L. Pleet, James Erickson, Catherine DeMarino, Robert A. Barclay, Maria Cowen, Benjamin Lepene, Janie Liang, Jens H. Kuhn, Laura Prugar, Spencer W. Stonier, John M. Dye, Weidong Zhou, Lance A. Liotta, M. Javad Aman, Fatah Kashanchi
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Virology
Piet Maes, Gaya K. Amarasinghe, Maria A. Ayllon, Christopher F. Basler, Sina Bavari, Kim R. Blasdell, Thomas Briese, Paul A. Brown, Alexander Bukreyev, Anne Balkema-Buschmann, Ursula J. Buchholz, Kartik Chandran, Ian Crozier, Rik L. de Swart, Ralf G. Dietzgen, Olga Dolnik, Leslie L. Domier, Jan F. Drexler, Ralf Duerrwald, William G. Dundon, W. Paul Duprex, John M. Dye, Andrew J. Easton, Anthony R. Fooks, Pierre B. H. Formenty, Ron A. M. Fouchier, Juliana Freitas-Astua, Elodie Ghedin, Anthony Griffiths, Roger Hewson, Masayuki Horie, Julia L. Hurwitz, Timothy H. Hyndman, Daohong Jiang, Gary P. Kobinger, Hideki Kondo, Gael Kurath, Ivan V. Kuzmin, Robert A. Lamb, Benhur Lee, Eric M. Leroy, Jianrong Li, Shin-Yi L. Marzano, Elke Muhlberger, Sergey V. Netesov, Norbert Nowotny, Gustavo Palacios, Bernadett Palyi, Janusz T. Paweska, Susan L. Payne, Bertus K. Rima, Paul Rota, Dennis Rubbenstroth, Peter Simmonds, Sophie J. Smither, Qisheng Song, Timothy Song, Kirsten Spann, Mark D. Stenglein, David M. Stone, Ayato Takada, Robert B. Tesh, Keizo Tomonaga, Noel Tordo, Jonathan S. Towner, Bernadette van den Hoogen, Nikos Vasilakis, Victoria Wahl, Peter J. Walker, David Wang, Lin-Fa Wang, Anna E. Whitfield, John V. Williams, Gongyin Ye, F. Murilo Zerbini, Yong-Zhen Zhang, Jens H. Kuhn
ARCHIVES OF VIROLOGY
(2019)
Article
Microbiology
Zachary A. Bornholdt, Andrew S. Herbert, Chad E. Mire, Shihua He, Robert W. Cross, Anna Z. Wec, Dafna M. Abelson, Joan B. Geisbert, Rebekah M. James, Niaz Rahim, Wenjun Zhu, Viktoriya Borisevich, Logan Banadyga, Bronwyn M. Gunn, Krystle N. Agans, Ariel S. Wirchnianski, Eileen Goodwin, Kevin Tierney, William S. Shestowsky, Ognian Bohorov, Natasha Bohorova, Jesus Velasco, Eric Ailor, Do Kim, Michael H. Pauly, Kevin J. Whaley, Galit Alter, Laura M. Walker, Kartik Chandran, Larry Zeitlin, Xiangguo Qiu, Thomas W. Geisbert, John M. Dye
CELL HOST & MICROBE
(2019)
Article
Multidisciplinary Sciences
Rohit K. Jangra, Andrew S. Herbert, Rong Li, Lucas T. Jae, Lara M. Kleinfelter, Megan M. Slough, Sarah L. Barker, Pablo Guardado-Calvo, Gleyder Roman-Sosa, M. Eugenia Dieterle, Ana I. Kuehne, Nicolas A. Muena, Ariel S. Wirchnianski, Elisabeth K. Nyakatura, J. Maximilian Fels, Melinda Ng, Eva Mittler, James Pan, Sushma Bharrhan, Anna Z. Wec, Jonathan R. Lai, Sachdev S. Sidhu, Nicole D. Tischler, Felix A. Rey, Jason Moffat, Thijn R. Brummelkamp, Zhongde Wang, John M. Dye, Kartik Chandran
Article
Multidisciplinary Sciences
Jennifer M. Brannan, Shihua He, Katie A. Howell, Laura I. Prugar, Wenjun Zhu, Hong Vu, Sergey Shulenin, Shweta Kailasan, Henna Raina, Gary Wong, Md Niaz Rahim, Logan Banadyga, Kevin Tierney, Xuelian Zhao, Yuxing Li, Frederick W. Holtsberg, John M. Dye, Xiangguo Qiu, M. Javad Aman
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Brandyn R. West, Anna Z. Wec, Crystal L. Moyer, Marnie L. Fusco, Philipp A. Ilinykh, Kai Huang, Ariel S. Wirchnianski, Rebekah M. James, Andrew S. Herbert, Sean Hui, Eileen Goodwin, Katie A. Howell, Shweta Kailasan, M. Javad Aman, Laura M. Walker, John M. Dye, Alexander Bukreyev, Kartik Chandran, Erica Ollmann Saphire
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2019)
Article
Virology
Brett F. Beitzel, Sheli R. Radoshitzky, Nicholas Di Paola, Jennifer M. Brannan, David Kimmel, Katie Caviness, Veronica Soloveva, Shuiqing Yu, Elena N. Postnikova, Courtney L. Finch, Hu Liu, Laura Prugar, Russell Bakken, John M. Dye, Jeffrey R. Kugelman, James M. Cunningham, Mariano Sanchez-Lockhart, Jens H. Kuhn, Gustavo Palacios
Summary: The study successfully developed a reverse-genetics system to produce recombinant stocks of Ebola virus variants, highlighting the crucial role of selecting and engineering emerging variants for addressing pressing public health and biosecurity concerns during Ebola outbreaks.
Article
Virology
Susan L. Uprichard, Amornrat O'Brien, Monika Evdokimova, Cynthia L. Rowe, Cara Joyce, Matthew Hackbart, Yazmin E. Cruz-Pulido, Courtney A. Cohen, Michelle L. Rock, John M. Dye, Paul Kuehnert, Keersten M. Ricks, Marybeth Casper, Lori Linhart, Katrina Anderson, Laura Kirk, Jack A. Maggiore, Andrew S. Herbert, Nina M. Clark, Gail E. Reid, Susan C. Baker
Summary: This study describes the antibody responses to mRNA vaccines in a cohort of 102 subjects, showing that a single exposure to antigen induces a variable antibody response affected by demographic factors and co-morbidities. The study also finds that COVID-19-experienced individuals achieve higher antibody levels and neutralization activity. Furthermore, COVID-19-naive individuals tend to have a stronger response to the Moderna vaccine compared to the Pfizer-BioNTech vaccine.