Article
Genetics & Heredity
Xianhui Ning, Li Sun
Summary: The study identified a large number of circRNAs in the spleen of Japanese flounder infected with V. anguillarum, with some of them being involved in immune-related pathways. Specific DEcirs were found to sponge targeted miRNAs that were differentially expressed during the infection, forming intricate immune-related networks with mRNAs and miRNAs. These findings suggest that circRNAs in flounder are regulated by V. anguillarum and may play important roles in immune defense against pathogen infection.
Article
Biotechnology & Applied Microbiology
Xianhui Ning, Li Sun
Summary: This study systematically identified and characterized flounder lncRNAs induced by V. anguillarum infection, revealing the crucial role of lncRNAs in host immune defense. The identified lncRNAs and ceRNA networks provide new insights into the anti-bacterial immunity of flounder.
Article
Fisheries
Jun Yang, Binghua Liu, Xiaohui Li, Guangling Li, Haishen Wen, Xin Qi, Yun Li, Feng He
Summary: Infection by Vibrio anguillarum can activate the NF-kappa B and TNF alpha pathway in the fish immune organs, leading to significant upregulation of these genes in the muscle of Japanese flounder. The expression of TNF alpha gene in the muscle is regulated by DNA methylation and its methylation level is negatively correlated with expression. The study highlighted the importance of understanding immune response mechanisms in fish breeding for disease resistance.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Xing, Xiaoyu Jiang, Hongsen Xu, Xiuzhen Sheng, Xiaoqian Tang, Heng Chi, Wenbin Zhan
Summary: Immunization with pVAA induces effective local and systemic immune responses in flounders, significantly increasing the proportion of CD4(+) cells and upregulating genes involved in immune system activation. The specific antibodies against L. anguillarum were induced post-immunization, reaching a peak at 4-5 weeks, with a relative percent survival rate of 53.85 +/- 3.64% in the pVAA group, suggesting the potential of pVAA for effective protection against L. anguillarum.
MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Xianghu Meng, Heng Chi, Zuobing Zhang, Qian Li, Xiuzhen Sheng, Xiaoqian Tang, Jing Xing, Wenbin Zhan
Summary: The study revealed the early immune response of flounder to inactivated V. anguillarum by detecting the transcriptome profiles. The hub module genes correlated with the immunization time were highly expressed in various immune-related aspects, including immune system processes, signal pathways, and cell differentiation.
Article
Fisheries
Baozhi Kuang, Kun Xiao, Wenqi Wang, Feng You, Peng Xiao, Shiyong Chen
Summary: This study analyzed the immunological properties and efficacies of bivalent inactivated vaccines in salmon. The results showed that the antigens from the two pathogenic strains had adjuvant effects on each other's vaccines. The proportions of antigens in the combined vaccines had different effects on immune response and protection against the specific pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Xueyan Shi, Heng Chi, Yuanyuan Sun, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Summary: This study characterized the morphology and composition of peritoneal cavity cells (PerC cells) in flounder. The resident PerC cells were found to consist of lymphocytes and myeloid cells. The composition of PerC cells changed rapidly after Vibrio anguillarum infection or immunization, with a non-specific stage dominated by phagocytes and a specific immune stage dominated by lymphocytes.
Article
Limnology
Hanlin Li, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: CCL4 and CCL19 can serve as molecular adjuvants for DNA vaccines, enhancing the vaccine's protective effect by stimulating local immune response, humoral immunity, and cellular immunity.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2022)
Article
Fisheries
Soon Ho Lee, Bo Ram Beck, Seok-Hong Hwang, Seong Kyu Song
Summary: This study presented a novel anti-Vibrio feed vaccine that can protect olive flounder from multiple strains of Vibrio pathogens. By activating immune responses, the vaccine significantly increased survival rates and weight gain rates in fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Wenjing Liu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study identified a CD80/86 homolog in flounder and investigated its expression characteristics in immune responses. The study revealed that CD80/86 is mainly found in antigen-presenting cells and participates in eliciting humoral immune responses through the CD80/86-CD28 signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Heng Chi, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Summary: MMP-9 is a protein belonging to the Matrix Metalloprotease family and plays crucial roles in various biological processes. This study investigated the characteristics and functions of the MMP-9 gene (PoMMP-9) in flounder, revealing its involvement in immune responses and tissue healing. The results suggest that PoMMP-9 is inducible and can recruit inflammatory cells through extracellular traps (ETs) for pathogen elimination and wound healing at inflammatory sites.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Wenting Cui, Jinhu Liu, Liang Cao, Shuozeng Dou
Summary: Seawater acidification and heavy metal pollutant cadmium (Cd) have significant effects on the immune and biomineralization functions of marine organisms, with Cd being more sensitive. Seawater acidification exacerbates the toxic effects of Cd exposure on immune and biomineralization, while high Cd exposure enhances their response to seawater acidification.
Article
Immunology
Jing Xing, Wenjing Liu, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: CD28 is cloned and characterized in flounder, with a high expression in various tissues. CD28+ cells are co-localized with CD4+ T lymphocytes, not with IgM+ B lymphocytes. Stimulation with keyhole limpet hemocyanin induces significant changes in CD28 expression and leads to increased cell proliferation and IL-2 production. Anti-CD28 Abs enhance T cell activation and modulate gene expression in cultured leukocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Wenwen Wang, Qian Xu, Xinbao Liu, Hongning Liu, Zhujun Li, Qingxin Fan, Suxu Tan, Kunpeng Shi, Yanting Xia, Zhenxia Sha
Summary: This study explored the relationship between bacterial concentrations and host mortality, inflammatory reaction, and immune response in half-smooth tongue sole after infection with Vibrio anguillarum. The results indicated that the 24-48 hour period post-infection was a key time node for early detection and intervention of vibriosis. Excessive activation of the complement system and inflammatory reaction may contribute to mass death in fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Chengcheng Liang, Xiuzhen Sheng, Xiaoqian Tang, Jing Xing, Heng Chi, Wenbin Zhan
Summary: This study identifies a specialized lymphoepithelial tissue called interbranchial lymphoid tissue (ILT) in fish species. The ILT's structural variation and mucosal immune functions are not well-known. The researchers found Zap-70(+) cell aggregates in the ILT, confirming its location in the epithelium covering the bottom of the interbranchial cleft. The ILT was found to be an important site for local T cell-mediated immunity in flounder's gills.
FISH & SHELLFISH IMMUNOLOGY
(2022)
