Article
Biochemistry & Molecular Biology
Yuan Gao, Xiaochen Gong, Shuang Yu, Zheng Jin, Qicheng Ruan, Chunjing Zhang, Kai Zhao
Summary: The study found that N-2-Hydroxypropyl trimethyl ammonium chloride chitosan/N, O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) showed good immunogenicity and no toxicity as a universal vaccine adjuvant/delivery system. The prepared N-2-HACC/OVA/CMCS NPs induced higher levels of antibodies and immune responses in mice, suggesting their potential in enhancing humoral, cellular, and mucosal immune responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Immunology
Mei Peng Tan, Wen Siang Tan, Noorjahan Banu Mohamed Alitheen, Wei Boon Yap
Summary: The development of a universal influenza vaccine addresses concerns related to annual reformulation and mismatching with circulating subtypes of current influenza vaccines. It contains conserved antigens for cross-protection against all influenza subtypes, with potential as a candidate for a universal influenza vaccine.
Article
Multidisciplinary Sciences
Ranmali Kavishna, Tha Yang Kang, Maurizio Vacca, Benson Yen Leong Chua, Hae-Young Park, Peck Szee Tan, Vincent Tk Chow, Mireille H. Lahoud, Sylvie Alonso
Summary: Influenza, also known as flu, is a global public health concern and a significant economic burden. Researchers have been pursuing the development of a broadly protective universal flu vaccine, and have now successfully improved the immunogenicity of the M2e vaccine using a targeting strategy. In mouse studies, this strategy has shown promising results in terms of generating a robust protective response. Translation to humans is now an exciting possibility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Daria Mezhenskaya, Irina Isakova-Sivak, Tatiana Kotomina, Victoria Matyushenko, Min-Chul Kim, Noopur Bhatnagar, Ki-Hye Kim, Sang-Moo Kang, Larisa Rudenko
Summary: Influenza viruses remain a serious public health problem, with seasonal influenza vaccines showing low effectiveness against drifted and new viruses. Research is ongoing to develop strategies to elicit immune responses against conserved parts of influenza virus proteins. A study constructing a universal live attenuated influenza vaccine (LAIV) candidate with enhanced breadth of protection showed promising results in increasing protection against heterosubtypic challenge viruses compared to classical LAIV. Further investigation in pre-clinical and clinical trials is warranted.
Article
Polymer Science
Yu Kang, Zhi Liu, Yingyun Long, Baolong Wang, Xu Yang, Di Sha, Kai Shi, Xiangling Ji, Bai Li, Yonggang Liu
Summary: A two-step method was used to synthesize N,N,N-trimethyl chitosan (TMC) from chitosan, with molecular weight ranging from 29 to 136 kg/mol and quaternization degree from 70% to 82%. Size exclusion chromatography (SEC) coupled with multiangle laser light scattering was employed to analyze the molecular weight and chain conformation, showing a significant increase in chain flexibility upon N-methylation, possibly due to the breakage of intramolecular hydrogen bonds between chitosan repeating units.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Ying Zhao, Shiyuan Lin, Ruiyue Fang, Yaling Shi, Wei Wu, Wei Zhang, Hui Chen
Summary: This study investigates the property and mechanism of intestinal absorption of nanoparticles coated with trimethyl chitosan derivatives (PPTT-NPs), which have previously been shown to enhance the oral bioavailability of panax notoginseng saponins. The results demonstrate that TMC-VB12 and TMC-Cys effectively promote the transport of PPTT-NPs, increasing their adhesion and permeation in the intestines. These findings suggest the potential for oral delivery of BCS III drugs.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Kessily B. Rufato, Paulo R. Souza, Ariel C. de Oliveira, Sharise B. R. Berton, Roberta M. Sabino, Edvani C. Muniz, Ketul C. Popat, Eduardo Radovanovic, Matt J. Kipper, Alessandro F. Martins
Summary: This study demonstrates that polysaccharide-based polyelectrolyte multilayers prepared using the layer-by-layer approach can provide stability and durability for free-standing gels, as well as antimicrobial properties and cytocompatibility.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Altaf H. Basta, Vivian F. Lotfy, May A. El-Manawaty, Khaled Mahmoud
Summary: Phenylalanine and lysine can enhance the safety of TMC against normal cell lines while providing high thermal stability. Phenylalanine shows superior performance in synthesizing anticancer agents.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2022)
Article
Chemistry, Multidisciplinary
Jingdi Pan, Qihui Wang, Mi Qi, Jianjun Chen, Xuefan Wu, Xiaowei Zhang, Wei Li, Xian-En Zhang, Zongqiang Cui
Summary: Researchers have developed an intranasal multivalent epitope-based nanoparticle vaccine that provides broad protection against diverse influenza A and B viruses. The vaccine induces potent immune responses, including cross-reactive antibodies and T cell-mediated responses, and confers full protection against lethal challenge with different influenza viruses. The HMNF nanoparticle demonstrates long-lasting immunity and shows promise as a universal influenza vaccine candidate.
Article
Biochemistry & Molecular Biology
Xiaoyi Gao, Nan Liu, Zengming Wang, Jing Gao, Hui Zhang, Meng Li, Yimeng Du, Xiang Gao, Aiping Zheng
Summary: This study modified chitosan nanoparticles with mannose and applied them to intranasal vaccine delivery. The results showed that mannose-modified chitosan nanoparticles enhanced antigen absorption and improved the immune response in mice. This research provides a promising method for further study of chitosan nanoparticle modification.
Article
Immunology
Sixin Zhang, Xinming Tang, Si Wang, Fangyun Shi, Chunhui Duan, Feifei Bi, Jingxia Suo, Dandan Hu, Jie Liu, Chaoyue Wang, Xun Suo, Xianyong Liu
Summary: The potential of Eimeria parasites as live vaccine vectors has been demonstrated by successful genetic manipulation on species like E. tenella, E. mitis, and E. necatrix. In this study, a plasmid containing fluorescent proteins and the H9N2 avian influenza virus M2e protein was transfected into E. acervulina, resulting in stable transgenic parasites. Future research will focus on the use of transgenic E. acervulina as a live vaccine vector.
Article
Biotechnology & Applied Microbiology
Kai Zhao, Beini Sun, Ci Shi, Yanwei Sun, Zheng Jin, Gaowei Hu
Summary: The O-2'-HACC/pFDNA microparticles showed high encapsulation efficiency and loading capacity, promoting immune responses and generating more immune-related cells after intranasal vaccination in chickens, providing better protection compared to commercial live attenuated vaccines.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Immunology
Kai Zhao, Yuan Gao, Gaowei Hu, Lei Wang, Shangjin Cui, Zheng Jin
Summary: The study developed a VP2 protein subunit vaccine against Porcine parvovirus (PPV) using N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) as the adjuvant, showing that the vaccine induced higher antibody levels and long-term immune protection in immunized sows. However, the vaccine mainly stimulated humoral immunity rather than cellular immunity, and provided 100% protective efficacy against PPV infection in sows. This suggests that N-2-HACC could be a potential adjuvant for controlling PPV.
Article
Virology
Daria Mezhenskaya, Irina Isakova-Sivak, Victoria Matyushenko, Svetlana Donina, Andrey Rekstin, Konstantin Sivak, Kirill Yakovlev, Anastasia Katelnikova, Kirill Kryshen, Valery Makarov, Larisa Rudenko
Summary: Experimental studies in a preclinical ferret model showed that the LAIV/HA+4M2e universal influenza vaccine candidate had higher immunogenicity and cross-protective potential, mainly mediated by M2e-targeted antibodies. Therefore, the LAIV/HA+4M2e vaccine is promising for further evaluation in future clinical trials.
Article
Pharmacology & Pharmacy
Kimberly Braz Gomes, Grace Lovia Allotey-Babington, Sucheta D'Sa, Sang-Moo Kang, Martin J. D'Souza
Summary: This study examined the activation status of dendritic cells (DCs) and the innate and adaptive immune responses after exposure to M2e VLP vaccine. Results showed that the M2e VLP microparticle had a strong stimulatory effect on DCs, and the presence of Alhydrogel(R) and MPL-A(R) adjuvants enhanced this effect.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Mehran Dabaghian, All Mohammad Latifi, Majid Tebianian, Fariba Dabaghian, Seyyed Mahmoud Ebrahimi
ANTIVIRAL RESEARCH
(2015)
Article
Biotechnology & Applied Microbiology
Yousef Amini, Majid Tebianian, Nader Mosavari, Mahdi Fasihi Ramandi, Seyyd Mahmoud Ebrahimi, Hamid Najminejad, Mehran Dabaghian, Meghdad Abdollahpour
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2017)
Article
Biochemical Research Methods
Seyyed Mahmoud Ebrahimi, Mehran Dabaghian, Mohammad Hossein Zabeh Jazi, Mashallah Mohammadi, Esmail Saberfar
Article
Parasitology
Seyyed Mahmoud Ebrahimi, Habib Paykari, Arash Memamejadian
EXPERIMENTAL PARASITOLOGY
(2013)
Article
Microbiology
Mehran Dabaghian, Ali Mohammad Latify, Majid Tebianian, Hassan Nili, Ali Reza Tevangar Ranjbar, Ali Mirjalili, Mashallah Mohammadi, Reza Banihashemi, Seyyed Mahmoud Ebrahimi
VETERINARY MICROBIOLOGY
(2014)
Article
Virology
Seyyed Mahmoud Ebrahimi, Mehran Dabaghian, Majid Tebianian, Mohammad Hossein Zabeh Jazi
Article
Virology
Mohammad Hossein Zabeh Jazi, Mehran Dabaghian, Majid Tebianian, Mohammad Javad Gharagozlou, Seyyed Mahmoud Ebrahimi
Article
Biotechnology & Applied Microbiology
Hamid Najminejad, Seyed Mehdi Kalantar, Ali Rezaei Mokarram, Mehran Dabaghian, Meghdad Abdollahpour-Alitappeh, Seyyed Mahmoud Ebrahimi, Majid Tebianian, Mandi Fasihi Ramandi, Mohammad Hasan Sheikhha
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2019)