Review
Biochemical Research Methods
Jiaqi Ren, Ning Wang, Piao Guo, Yanping Fan, Francis Lin, Jiandong Wu
Summary: Cell migration plays a crucial role in biological processes. Microfluidics has revolutionized the research in this field by reducing costs and allowing precise manipulation of parameters affecting cell migration. Recent developments include better control of the cellular microenvironment, application-oriented models, and machine-assisted analysis methods. This review discusses co-culture models, spatiotemporal manipulation of chemical gradients, organ-on-chip models, and deep learning image processing strategies for cell migration. The challenges and future perspectives of using microfluidic techniques to study cell migration are also discussed.
Article
Nanoscience & Nanotechnology
Yonger Xue, Rafia Baig, Yizhou Dong
Summary: Stem cells, used as 'living drugs' for decades, offer potential solutions for various diseases, but face challenges in clinical translation due to pathological environments. To address these challenges, researchers have developed biomaterial-based strategies to enhance stem cell therapies for the next generation.
Review
Immunology
Hong You, Malcolm K. Jones, Deanne J. Whitworth, Donald P. McManus
Summary: Schistosomes, parasites causing schistosomiasis, have a complex life cycle involving infection of freshwater snails and mammals. Stem cells play a key role in facilitating adaptation to new environments and reproductive processes in different life cycle stages of the parasite. Establishment of stem cell lines in schistosomes may provide valuable resources for studying stem cell biology and developing new intervention strategies for schistosomiasis.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Fangyuan Shen, Yu Shi
Summary: The article reviews the research progress of single-cell RNA sequencing technology in the identification of osteogenic markers and differentiation pathways, as well as the application of single-cell technology combined with experimental techniques in MSC-related new insights. It also summarizes the latest findings regarding the interaction between stem cell fate and the microenvironment in homeostasis and pathological processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Endocrinology & Metabolism
You Jeong Park, Cesar Borlongan
Summary: Over the past 50 years, stem cell therapy has made significant progress in treating brain disorders, particularly stroke, as it has moved from laboratory research to clinical applications. The increased incidence of stroke in COVID-19 survivors has highlighted the potential of stem cells' anti-inflammatory properties in treating the condition. Optimizing stem cell transplant protocols is crucial for ensuring safe and effective clinical application in stroke and related neurological disorders.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Review
Biology
Xin Fu, Qiang He, Yu Tao, Mengdi Wang, Wei Wang, Yalong Wang, Qing Cissy Yu, Fang Zhang, Xiaoyu Zhang, Ye-Guang Chen, Dong Gao, Ping Hu, Lijian Hui, Xiaoqun Wang, Yi Arial Zeng
Summary: Stem cells are crucial undifferentiated cells with the ability of self-renewal and differentiation, playing a significant role in organ and tissue development and repair. Tissue-specific stem cells hold powerful potential for regeneration and are promising for future research in tissue stem cell biology.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Review
Materials Science, Biomaterials
Yu Chen, Samira Pal, Quanyin Hu
Summary: Cell-based medicines have attracted attention for biomedical applications due to their low immunogenicity, compatibility, and ability to cross biological barriers. However, challenges such as preparation, stability, manufacturing, toxicity, and affordability need to be addressed for their clinical translation. The development of biotechnology and engineering approaches in materials sciences offers a platform to overcome these issues.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Oncology
Troy A. McEachron, Lee J. Helman
Summary: In recent years, there has been a significant shift in the field of pediatric cancer research, focusing on genetic and epigenetic drivers of disease, cellular origins of different pediatric cancers, disease models, the tumor microenvironment, and cellular immunotherapies.
Review
Immunology
Masahiro Miyata, Kazunobu Ichikawa, Eri Matsuki, Masafumi Watanabe, Daniel Peltier, Tomomi Toubai
Summary: Acute kidney injury is a common complication of allogeneic hematopoietic cell transplantation and can be caused by multiple factors. Recent studies suggest that graft-versus-host disease may attack the kidneys and contribute to the development of renal injury.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Medicine, Research & Experimental
Yongqiang Dong, Xudong Wu, Xuerong Chen, Ping Zhou, Fangming Xu, Wenqing Liang
Summary: Stem cell nanotechnology, an emerging field, has made significant progress in experimental studies, confirming the importance of nanotechnology in stem cell therapies. The structure and properties of nanomaterials impact the proliferation and differentiation of stem cells, leading to a new interdisciplinary frontier in material science and regenerative medicine.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Biochemistry & Molecular Biology
Yuanwang Pan, Han Han, Kristen E. Labbe, Hua Zhang, Kwok-Kin Wong
Summary: Lung squamous cell carcinoma (LUSC) is a major subtype of non-small cell lung cancer with limited treatment options. Immunotherapy has become the new standard of care for LUSC, but targeted driver mutations are lacking. Researchers believe that improved modeling systems are needed to gain a better understanding of the biology of LUSC.
Review
Biotechnology & Applied Microbiology
Fumei He, Jinxiu Cao, Junyang Qi, Zeqi Liu, Gan Liu, Wenbin Deng
Summary: Inorganic nanomaterials have unique physical and chemical properties that can optimize the therapeutic effects of stem cell transplantation and influence stem cell differentiation. They can be used as vectors to transport substances that interfere with the fate of stem cells, due to their huge specific surface area and modifiability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Nathanael Andrews, Jason T. Serviss, Natalie Geyer, Agneta B. Andersson, Ewa Dzwonkowska, Iva Sutevski, Rosan Heijboer, Ninib Baryawno, Marco Gerling, Martin Enge
Summary: This study introduces a new method called CIM-seq, which can analyze cell-cell interactions in different tissues by sequencing cell multiplets. The results demonstrate that CIM-seq is applicable for unsupervised profiling of cell-type interactions in different tissue types.
Review
Engineering, Biomedical
Zhongqiao Gan, Xinyuan Qin, Haitao Liu, Jiayue Liu, Jianhua Qin
Summary: Organoids are in vitro model systems that mimic the complexity of organs with multicellular structures and functions, which provide great potential for biomedical and tissue engineering. However, their current formation heavily relies on using complex animal-derived extracellular matrices (ECM), such as Matrigel. These matrices are often poorly defined in chemical components and exhibit limited tunability and reproducibility. Recently, the biochemical and biophysical properties of defined hydrogels can be precisely tuned, offering broader opportunities to support the development and maturation of organoids.
BIOACTIVE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Zhengjun Lin, Yanlin Wu, Yiting Xu, Guoqing Li, Zhihong Li, Tang Liu
Summary: This article systematically summarizes the roles and detailed molecular mechanisms of mesenchymal stem cell-derived exosomes (MSC-derived exosomes) in mediating cancer therapy resistance, providing novel insights into the clinical applications of MSC-derived exosomes in cancer management.
Review
Cell Biology
Ryo Seishima, Nick Barker
Article
Oncology
Yuezhen Xue, Nick Barker, Shawn Hoon, Pingping He, Teena Thakur, Shifana Raja Abdeen, Priadarsini Maruthappan, Farid J. Ghadessy, David P. Lane
Article
Biochemistry & Molecular Biology
Nawaphat Jangphattananont, Hiroki Sato, Ryu Imamura, Katsuya Sakai, Yumi Terakado, Kazuhiro Murakami, Nick Barker, Hiroko Oshima, Masanobu Oshima, Junichi Takagi, Yukinari Kato, Seiji Yano, Kunio Matsumoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Multidisciplinary Sciences
Ryo Seishima, Carly Leung, Swathi Yada, Katzrin Bte Ahmed Murad, Liang Thing Tan, Amin Hajamohideen, Si Hui Tan, Hideki Itoh, Kazuhiro Murakami, Yoshihiro Ishida, Satoshi Nakamizo, Yusuke Yoshikawa, Esther Wong, Nick Barker
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Si Hui Tan, Yada Swathi, Shawna Tan, Jasmine Goh, Ryo Seishima, Kazuhiro Murakami, Masanobu Oshima, Toshikatsu Tsuji, Phyllis Phuah, Liang Thing Tan, Esther Wong, Aliya Fatehullah, Taotao Sheng, Shamaine Wei Ting Ho, Heike Grabsch, Supriya Srivastava, Ming Teh, Simon L. I. J. Denil, Seri Mustafah, Patrick Tan, Asim Shabbir, Jimmy So, Khay Guan Yeoh, Nick Barker
Article
Multidisciplinary Sciences
Kazuhiro Murakami, Yumi Terakado, Kikue Saito, Yoshie Jomen, Haruna Takeda, Masanobu Oshima, Nick Barker
Summary: Safe utilization of the regenerative potential of adult stem cells for clinical applications requires a comprehensive understanding of the regulatory mechanisms. Epithelial organoid cultures accurately mimic in vivo stem cell-driven epithelial renewal, offering a great ex vivo platform for studying key regulatory mechanisms. Through genome-scale CRISPR knockout screening, Alk, Bclaf3, and Prkra were identified as potential regulators of gastric epithelial differentiation and could suppress stemness/proliferation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Carly Leung, Katzrin Bte Ahmad Murad, Adelyn Liang Thing Tan, Swathi Yada, Sowmya Sagiraju, Peter Karl Bode, Nick Barker
Editorial Material
Gastroenterology & Hepatology
Hui Yi Grace Lim, Nick Barker
Article
Multidisciplinary Sciences
Yuezhen Xue, Boris San Luis, Regine J. Dress, Katzrin Binte Ahmad Murad, Florent Ginhoux, Nick Barker, David Lane
Summary: This study demonstrates that the proteasome inhibitor bortezomib stabilizes p53 in stem and progenitor cells in gastrointestinal and lymphoid tissues. The stabilization of p53 is observed in various hematopoietic stem and progenitor cells, as well as T cells and B cells. Understanding the regulation of p53 in these cells is important for developing strategies for cancer treatment and immunotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Developmental Biology
Fidelia B. Alvina, Tanysha Chi-Ying Chen, Hui Yi Grace Lim, Nick Barker
Summary: This article provides a comprehensive synthesis of the existing knowledge on gastric epithelial stem cell biology, including the application of experimental techniques. It highlights the role of stem/progenitor cells in gastric development, cell differentiation, and homeostasis maintenance, and discusses the gaps in understanding and future research directions.
Article
Biochemistry & Molecular Biology
Atsushi Takahashi-Kanemitsu, Mengxue Lu, Christopher Takaya Knight, Takayoshi Yamamoto, Takuo Hayashi, Yusuke Mii, Takuya Ooki, Ippei Kikuchi, Akira Kikuchi, Nick Barker, Etsuo A. Susaki, Masanori Taira, Masanori Hatakeyama
Summary: Helicobacter pylori strains that deliver the oncoprotein CagA into gastric epithelial cells play a major role in causing upper gastric diseases, including gastric cancer. This study shows that CagA disrupts Wnt-dependent planar cell polarity (Wnt/PCP), which coordinates collective cell behaviors such as convergent extension and contributes to epithelial elongation. The expression of CagA in Xenopus laevis embryos and specific expression in the stomach epithelium of mice resulted in impaired development processes driven by Wnt/PCP signaling. In human gastric epithelial cells, CagA interacts with VANGL1/2, leading to the mislocalization of VANGL1/2 and impairment of Wnt/PCP signaling. These findings suggest that CagA may contribute to gastric cancer development by promoting pyloric gland stem cell proliferation and inhibiting enteroendocrine differentiation, through subverting the Wnt/PCP-dependent mechanism.
Meeting Abstract
Biochemistry & Molecular Biology
T. Naert, E. Szenker-Ravi, U. Altunoglu, M. Leushacke, C. Bosso-Lefevre, M. Khatoo, T. Hong, R. Noelanders, A. Hajamohideen, C. Beneteau, S. Bernardo de Sousa, B. Karaman, X. Latypova, S. Basaran, E. Borklu Yucel, T. Thong, L. Vlaeminck, S. S. Nayak, G. Katta Mohan, C. Le Caignec, N. Soshnikova, Z. O. Uyguner, N. Barker, H. Kayserili, K. Vleminckx, B. Reversade
EUROPEAN JOURNAL OF HUMAN GENETICS
(2019)
Article
Biochemistry & Molecular Biology
Dustin Flanagan, Nick Barker, Matthias Ernst, Elizabeth Vincan, Toby Phesse
