Article
Engineering, Biomedical
Akihito Miyamoto, Hiroshi Kawasaki, Sunghoon Lee, Tomoyuki Yokota, Masayuki Amagai, Takao Someya
Summary: A method is reported for continuous monitoring of skin electrical resistance and transepidermal water loss, which can evaluate the barrier function of the skin and reflect the dynamic changes of skin physiology.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Allergy
Arturo O. Rinaldi, Manru Li, Elena Barletta, Paolo D'Avino, Duygu Yazici, Yagiz Pat, Siobhan Ward, Daniel Burla, Ge Tan, Nima Askary, Rasmus Larsson, Jeremy Bost, Huseyn Babayev, Raja Dhir, Nicolas Gaudenzio, Mubeccel Akdis, Kari Nadeau, Cezmi A. Akdis, Yasutaka Mitamura
Summary: The study demonstrated that household laundry detergents and SDS impaired the epidermal barrier in both in vivo and ex vivo human skin models. Daily exposure to detergents may lead to skin barrier disruption and contribute to the development of atopic diseases. Laundry detergents caused reduced electrical impedance, disrupted major pathways involved in skin barrier integrity, and induced inflammation.
Article
Dermatology
Fatemeh Keshavarzi, Saeed Zajforoushan Moghaddam, Malene Barre Pedersen, Nina Ostergaard Knudsen, Shadi Jafarzadeh, Esben Thormann
Summary: This study developed an in vitro setup to predict the breathability of different polymeric film formers on human skin successfully. The in vivo experiment on ten volunteers validated the results of the in vitro study, assisting in selecting suitable film-forming polymers for topical formulations.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Nutrition & Dietetics
Teik Kee Leo, Eugenie Sin Sing Tan, Farahnaz Amini, Navedur Rehman, Edmond Siah Chye Ng, Chung Keat Tan
Summary: Oral supplementation of rice ceramides (RC) can significantly improve skin hydration, firmness and elasticity, reduce wrinkle severity, and decrease levels of melanin and erythema index.
Article
Biophysics
Ziliang He, Benjin Zhang, Jing Zhang, Zhenghua Xiao, Ling He, Gang Yang
Summary: The study developed a prototype of a neonatal wearable device that can wirelessly monitor physiological parameters during phototherapy. The preliminary experiments demonstrated the effectiveness of simultaneous monitoring. This device has the potential to contribute to phototherapy nursing guidelines and physiological monitoring standards.
PHYSIOLOGICAL MEASUREMENT
(2023)
Review
Dermatology
Tanja Klotz, Abdullah Ibrahim, Guy Maddern, Yugesh Caplash, Marcus Wagstaff
Summary: This review examined the reliability and measurement error of devices measuring transepidermal water loss (TEWL). The study found that TEWL measurement devices demonstrate good reliability but have variable measurement errors, which improve under in vitro conditions. Future research should consider risk of bias factors when designing studies.
SKIN RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Emilia Alves, Joao Gregorio, Andre Rolim Baby, Patricia Rijo, Luis M. Rodrigues, Catarina Rosado
Summary: Diet plays a fundamental role in maintaining the balance of bodily functions, including skin health, through the gut-skin axis. Probiotics in kefir have potential therapeutic benefits for inflammatory skin diseases like atopic dermatitis. The intake of homemade kefir has been shown to improve skin barrier function and severity of atopic dermatitis, particularly in terms of skin hydration.
Review
Dermatology
Maxwell Green, Aileen M. Feschuk, Nadia Kashetsky, Howard Maibach
Summary: This review summarizes the variables that may cause variation in TEWL. The current regulatory agencies have failed to control for additional variables of interest, therefore defining normal TEWL may currently be problematic. Researchers should continue to examine factors impacting individual skin integrity.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Medicine, General & Internal
Trinidad Montero-Vilchez, Juan-Angel Rodriguez-Pozo, Pablo Diaz-Calvillo, Maria Salazar-Nievas, Jesus Tercedor-Sanchez, Alejandro Molina-Leyva, Salvador Arias-Santiago
Summary: The study aimed to evaluate the impact of dupilumab on skin barrier function in patients with atopic dermatitis (AD) and compare it with other treatments. The study found that dupilumab was more effective in improving barrier function in both eczematous lesions and non-lesioned skin compared to topical corticosteroids and cyclosporine.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Alvin Berger
Summary: DF-5 oil has anti-inflammatory potential to accelerate healing and improve skin barrier function. It also has the potential to enhance skin hydration and elasticity in normal skin.
LIPIDS IN HEALTH AND DISEASE
(2022)
Article
Medicine, General & Internal
Trinidad Montero-Vilchez, Maria-Victoria Segura-Fernandez-Nogueras, Isabel Perez-Rodriguez, Miguel Soler-Gongora, Antonio Martinez-Lopez, Ana Fernandez-Gonzalez, Alejandro Molina-Leyva, Salvador Arias-Santiago
Summary: This study compared skin barrier function between healthy skin, psoriatic skin, and atopic dermatitis skin, finding that TEWL was higher at psoriatic and AD lesions, while SCH was lower. Specific TEWL and temperature values may help clinicians determine disease severity and treatment needs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Dermatology
Elizabeth Tarshish, Karin Hermoni, Yoav Sharoni, Philip W. Wertz, Nava Dayan
Summary: The study found that golden tomato extract can enhance innate immunity and other defense mechanisms in the dermis, as well as providing antioxidants to optimize TEWL and skin appearance.
JOURNAL OF COSMETIC DERMATOLOGY
(2022)
Article
Dermatology
Trinidad Montero-Vilchez, Antonio Martinez-Lopez, Carlos Cuenca-Barrales, Andrea Rodriguez-Tejero, Alejandro Molina-Leyva, Salvador Arias-Santiago
Summary: Wearing masks and gloves may impair skin homeostasis and epidermal barrier function, leading to increased transepidermal water loss and higher skin temperature. High-quality personal protective equipment should be provided, and appropriate skin prevention measures should be implemented.
Article
Multidisciplinary Sciences
Lars Ruether, Werner Voss
Summary: Five different galenics were analyzed for tissue breathability and gas exchange after 6 hours of application on pig ear skin. Hydrogel preparations showed higher breathability and moisture penetration, while Tyrosur (R) Care Expert Wound Gel and ointment exhibited sustained moisturizing effects.
Article
Chemistry, Medicinal
Nuria Garros, Paola Bustos-Salgados, Oscar Domenech, Maria Jose Rodriguez-Lagunas, Negar Beirampour, Roya Mohammadi-Meyabadi, Mireia Mallandrich, Ana C. C. Calpena, Helena Colom
Summary: Atopic dermatitis (AD) is a chronic autoimmune inflammatory skin disorder. This study aimed to develop and evaluate new topical liposomal formulations loaded with the reversible Janus-associated kinase (JAK)-inhibitor, Baricitinib (BNB), for the treatment of flare ups. Three liposomal formulations were prepared using different proportions of POPC, CHOL, and CER. Physicochemical characterization, release study, permeation and retention studies, histological analysis, and irritancy tests were performed. All liposomes showed good properties and stability. The POPC:CHOL:CER formulation had the highest flux and permeation, with retention in the skin equal to POPC:CHOL. The formulations exhibited no harmful effects and showed promising results for the aim of the study.
Article
Pharmacology & Pharmacy
Lama Alsheddi, Kavssery Ananthapadmanabhan, S. Kevin Li
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Pharmacology & Pharmacy
Terri D. La Count, Qian Zhang, Michael Murawsky, Jinsong Hao, Priyanka Ghosh, Kaushalkumar Dave, Sam G. Raney, Arjang Talattof, Gerald B. Kasting, S. Kevin Li
Article
Chemistry, Medicinal
Qian Zhang, Michael Murawsky, Terri D. LaCount, Jinsong Hao, Priyanka Ghosh, Sam G. Raney, Gerald B. Kasting, S. Kevin Li
JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Article
Chemistry, Multidisciplinary
Kamchai Saepang, S. Kevin Li, Doungdaw Chantasart
Summary: This study evaluated the effect of decreasing the formulation pH to increase the positive charges of pramipexole dihydrochloride (PXCl) on its iontophoretic transport across skin. The results showed that the decrease in PXCl delivery at higher valence at pH 3 was mainly due to pH-dependent selectivity of PX ion permeation across the skin and not electroosmosis. Skin permselectivity is a significant factor for iontophoretic transport of PXCl, and reducing formulation pH to increase the positive charges on PX ions did not enhance PXCl delivery.
PHARMACEUTICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Kamchai Saepang, S. Kevin Li, Doungdaw Chantasart
Summary: The study explored the potential of skin microchannels created by polymer microneedle patches for enhancing transdermal drug delivery of pramipexole dihydrochloride. Results showed that drug-loaded dissolving microneedle (DMN) patches greatly improved PXCl transdermal delivery compared to conventional methods, while hydrogel-forming microneedle (HGMN) patches provided more sustained drug delivery. Electromigration was identified as the main mechanism for iontophoresis of PXCl through microchannels, with HGMN patches showing greater flux enhancement than DMN patches. The study indicates the potential application of microneedle patches, either alone or in combination with iontophoresis, as an alternative method for PXCl administration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Kamchai Saepang, S. Kevin Li, Doungdaw Chantasart
Summary: This study examined the effect of PDC iontophoresis on transdermal delivery of PXCl and found that while PDC iontophoresis can modulate the permeation profile of PXCl, it does not enhance iontophoretic transport compared to continuous DC iontophoresis.
PHARMACEUTICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Apipa Wanasathop, Priya B. Patel, Hyojin A. Choi, S. Kevin Li
Summary: An in-depth review of permeability data of porcine buccal mucosa revealed significant variability, with correlations observed between permeability coefficients and factors such as octanol/water partition coefficients, molecular weight, and effective pore radius for the buccal membrane barrier.
Article
Pharmacology & Pharmacy
Apipa Wanasathop, Michael Murawsky, S. Kevin Li
Summary: This study evaluated a 3D-printed small volume flow-through dissolution chamber system, modified for biorelevant and dose-discriminating testing of periodontal drug products. The results showed that, except for Atridox, there were no significant differences in the drug release profiles in vitro and in vivo. The drug release data demonstrated dose-discriminating ability at specific time points.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Materials Science, Biomaterials
Thomas Waterkotte, Xingyu He, Apipa Wanasathop, S. Kevin Li, Yoonjee C. Park
Summary: The study suggests that monoclonal antibodies can be released from a porous PCL capsule without being affected by polymer degradation, potentially lasting for up to 6 months. Accelerated degradation can determine long-term release kinetics within 14 days.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Chemistry, Medicinal
Apipa Wanasathop, Cheng Zhong, Patcharawan Nimmansophon, Michael Murawsky, S. Kevin Li
Summary: Gingiva, or gum, is a protective barrier for the tooth, preventing mechanical and bacterial damage. It is a target for topical drugs and understanding its barrier properties is important for effective drug delivery. A study using porcine gingiva as a model determined the permeability coefficients and observed a correlation between the Log P and Log Dow.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Medicinal
Patcharawan Nimmansophon, Apipa Wanasathop, S. Kevin Li
Summary: Membrane lateral transport has been studied in diffusion cells under edge effect and membrane blocking conditions. The simulations showed that edge effect could increase the flux across the membrane, especially with a thick membrane and small cell opening. Lateral diffusion within the membrane can mitigate the impact of membrane blocking by an object.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Toxicology
Cheng Zhong, Apipa Wanasathop, Zhanquan Shi, Kavssery Ananthapadmanabhan, S. Kevin Li
Summary: In vitro models can be used as alternatives to the Draize eye irritation test, but most alternative models lack quantitation to assess the effects of chemicals on the corneal barrier. This study investigates tissue electrical resistance as a sensitive in vitro testing approach to evaluate tissue alteration caused by chemicals in pharmaceutical and cosmetic products. The results show that the tested models can provide quantitative assessments of eye-irritating chemicals.
TOXICOLOGY IN VITRO
(2022)
Article
Pharmacology & Pharmacy
Apipa Wanasathop, Hyojin Alex Choi, Patcharawan Nimmansophon, Michael Murawsky, Deepak G. Krishnan, S. Kevin Li
Summary: The permeability of human gingiva was evaluated and compared with that of porcine gingiva. Fresh human and porcine gingiva showed similarities in permeability coefficients and octanol-water distribution coefficients. Porcine gingiva had lower permeability than human gingiva and the permeability increased significantly after freezing. Frozen human cadaver tissue could not be used due to high permeability and sample-to-sample variabilities.
Article
Nanoscience & Nanotechnology
Xingyu He, Zheng Yuan, Samantha Gaeke, Winston W-Y Kao, S. Kevin Li, Daniel Miller, Basil Williams, Yoonjee C. Park
Summary: This study successfully developed a dosage-controllable drug delivery implant using light-activated liposomes and near-infrared laser. The drug release was controllable both in vitro and in vivo, with no abnormality observed in the eyes treated with the implant, suggesting the safety and potential efficacy of this system for long-term treatment of posterior eye diseases.
ACS APPLIED BIO MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Xingyu He, Zheng Yuan, Winston Kao, Daniel Miller, S. Kevin Li, Yoonjee C. Park
ACS APPLIED BIO MATERIALS
(2020)
Article
Toxicology
Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
Summary: This study developed a new method to assess the skin irritation of antimicrobial cleaning products. The method utilized a more human-like model and demonstrated its effectiveness through comparison with in vivo rabbit skin irritation data.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Arupam Patra, Thirukumaran Kandasamy, Siddhartha Sankar Ghosh, Gurvinder Kaur Saini
Summary: This study successfully produced recombinant anisoplin and demonstrated its significant anti-cancer effect and ability to induce apoptosis in breast cancer cells. The activation of related signaling pathways may be the key to cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Summary: This study used live cell imaging to observe the accumulation of amiodarone in primary rat alveolar type II cells, and found that it specifically accumulates in lamellar bodies. The uptake is rapid, while storage is persistent. The main mechanisms for intracellular bioaccumulation of amiodarone are proposed to be passive diffusion, ion-trapping, and lipophilic interactions.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Filiz Bakar-Ates, Erva Ozkan
Summary: This study investigated the ferroptotic effect of CuB in breast cancer cells and evaluated its combination with erastin, a ferroptosis inducer. The results showed that the combination treatment significantly activated the ferroptotic pathways and altered the expression of iron-related proteins in breast cancer cells.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Rachel Z. Bennie, Ian C. Shaw
Summary: Estrogen mimics are synthetic and naturally occurring compounds that can interact with estrogen receptors in animals. In vitro transactivation reporter gene assay and in silico molecular modelling can be used to predict the mimicry of these compounds, reducing reliance on animal studies.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Sevda Isik, Semra Cicek
Summary: This study found that high doses of riboflavin can cause cytotoxicity in rainbow trout gonad cells and affect the transcriptional expressions of antioxidant enzymes and growth and reproductive genes, potentially leading to DNA damage and cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Ying Wang, Jie Wu, Mengqi Wan, Dou Yang, Fasheng Liu, Kehao Li, Manxin Hu, Yuanyuan Tang, Huiqiang Lu, Shouhua Zhang, Yuanzhen Xiong
Summary: m-Cresol is commonly used as an intermediate for pesticides and other industrial applications. This study investigated the hepatotoxicity of m-cresol using zebrafish larvae and explored its molecular mechanisms. The results suggest that m-cresol may induce liver damage in zebrafish larvae through oxidative stress and cell apoptosis pathways.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Vincent J. Manna, Shannon Dwyer, Vanessa Pizutelli, Salvatore J. Caradonna
Summary: The widespread use of electronic cigarettes and the emergence of a new illness have raised concerns about the effects of e-cigarette vapor on respiratory tissues. Researchers have developed a simple device to mimic the response of human airway tissue after long-term exposure to e-cigarette vapor, and have identified differences in the effects of different vapor compositions on airway tissue.
TOXICOLOGY IN VITRO
(2024)