Article
Cell & Tissue Engineering
Naoto Honda, Yasuhiro Watanabe, Yuta Tokuoka, Ritsuko Hanajima
Summary: This study investigated the roles of microglia, macrophages, and acquired antibodies in cell sheet transplantation in a mouse model. In vitro experiments showed that MSCs induced M2 polarization in macrophages and promoted their migration. Despite the favorable characteristics of microglia and macrophages, their elimination improved the survival of donor cells in vivo.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Oksana Y. Dudaryeva, Aurelia Bucciarelli, Giovanni Bovone, Florian Huwyler, Shibashish Jaydev, Nicolas Broguiere, Marwa al-Bayati, Marco Lutolf, Mark W. Tibbitt
Summary: The biophysical properties of the cellular microenvironment, such as stiffness and geometry, have been shown to influence cell function, with recent findings implicating 3D confinement as a significant regulator of cell behavior. This study demonstrates that the extent of 3D confinement affects cell viability and proliferation, leading to changes in nuclear morphology and YES-associated protein (YAP) localization. The effects of confinement on cell behavior scale with the degree of 3D confinement, highlighting the importance of 3D confinement as a regulator of cell function.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Genetics & Heredity
Xianmei Zhong, Jie Chen, Bo Wen, Xu Wu, Mingxing Li, Fukuan Du, Yu Chen, Shuai Deng, Yueshui Zhao, Jing Shen, Zhangang Xiao
Summary: Immunosenescence, characterized by immunodeficiency and inflammation, occurs with age. This review explores the potential role of mesenchymal stem cells (MSCs) in protecting against T cell aging, providing new insights for intervening in immune system aging.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Cell & Tissue Engineering
O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. Neganova
Summary: This study investigated the changes occurring within 3D-cultured MSCs and found that 3D-MSCs were able to lose replicative senescence markers, undergo changes in cytoskeleton structure and Golgi apparatus architecture, and exhibit nuclear localization of mTOR. The study also demonstrated that 3D cell culture promotes the phenotypic reversion of senescent cells rather than their removal from the population.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biology
Seung Yeon Lee, Jin Woo Lee
Summary: This article reviews the recent advances in the application of 3D culture techniques for cartilage regeneration, focusing on the use of 3D spheroid culture system using stem cells. Exosomes, as intercellular communication systems, play a role in promoting chondrogenic differentiation and proliferation for cartilage repair. Further mechanistic studies are needed to enhance the therapeutic options for osteoarthritis using MSC-derived exosomes.
Article
Multidisciplinary Sciences
Nadja Engel, Carsten Fechner, Annika Voges, Robert Ott, Jan Stenzel, Stefan Siewert, Carina Bergner, Valeria Khaimov, Jan Liese, Klaus-Peter Schmitz, Bernd Joachim Krause, Bernhard Frerich
Summary: Efficient and homogeneous seeding of stem cells in bone substitute scaffolds was achieved using a newly designed bioreactor with a conical diffuser geometry. Optimization of flow rates, cell density, and perfusion properties improved the distribution homogeneity throughout the scaffold.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Soraya Savoj, Mohammad Hossein Nasr Esfahani, Akbar Karimi, Fereshteh Karamali
Summary: This study assessed the inducing activity of SCAP in the differentiation of hESCs into retinal cells in a 3D organoid system. Increasing the number of SCAPs improved the formation of the optic vesicle structure.
Review
Immunology
Xi Liu, Qian Wei, Lu Lu, Shengnan Cui, Kui Ma, Wenhua Zhang, Fang Ma, Haihong Li, Xiaobing Fu, Cuiping Zhang
Summary: Inflammatory diseases caused by immune system disorders are a major public health concern. Mesenchymal stem cell derived extracellular vesicles (MSC-EVs) have shown great potential in immune modulation and offer a promising solution for the treatment of inflammatory diseases. Despite being in the early stages of research, MSC-EVs have been found to regulate the activities of innate and adaptive immune cells and have been tested in clinical trials for inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Anne Wolff, Marcus Frank, Susanne Staehlke, Kirsten Peters
Summary: The cultivation conditions are crucial for the behavior of adMSC cells, as adipogenic differentiation only occurs under specific stimulation. Compared to 2D culture, the 3D culture environment has significant effects on adipogenic differentiation and adipokine release.
Article
Biochemistry & Molecular Biology
Anne Wolff, Marcus Frank, Susanne Staehlke, Armin Springer, Olga Hahn, Juliane Meyer, Kirsten Peters
Summary: Mesenchymal stem/stromal cells (MSC) have the ability to renew or differentiate into tissue-specific cells. Comparing the effects of 2D and 3D culture on osteogenic differentiation, it was found that the 3D environment can accelerate and enhance the differentiation process, as well as release factors affecting bone metabolism.
Review
Cell & Tissue Engineering
Mesude Bicer, Graeme S. Cottrell, Darius Widera
Summary: As populations age, osteoporosis and osteoporosis-related fractures are becoming increasingly prevalent degenerative bone diseases. Three-dimensional cell culture technology may serve as an alternative therapeutic approach to tackle osteoporosis, allowing for more physiological expansion and differentiation of stem cells.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Immunology
Fen Zhang, Linxiao Fan, Qiuhong Liu, Shima Tang, Sainan Zhang, Lanlan Xiao, Lingjian Zhang, Qian Li, Nueraili Maihemuti, Lanjuan Li
Summary: MenSCs treatment can alleviate autoimmune hepatitis by inhibiting macrophages and M1 polarization in the liver tissue.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Valentina Giudice, Pasqualina Scala, Rosa Vitolo, Tina Patricia Dale, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study investigated the chondrogenic potentials of 3D high-density cultures of bone marrow and Wharton's Jelly-derived mesenchymal stromal cells (MSCs) supplemented with hTGF-beta 1. The study found that high-density cultures promoted chondrogenesis and that Wharton's Jelly-derived MSCs showed potential for chondrogenic commitment. These findings have important implications for cartilage regeneration and tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ting-Wei Kao, Arthur Chiou, Keng-Hui Lin, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
Summary: Research shows that mechanical stimulation affects the intracellular viscoelasticity of human mesenchymal stem cells, with cells cultured in rigid matrix displaying higher elastic and viscous moduli. This effect remains consistent throughout the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Pasqualina Scala, Paola Manzo, Erwin Pavel Lamparelli, Joseph Lovecchio, Maria Camilla Ciardulli, Valentina Giudice, Carmine Selleri, Emanuele Giordano, Laura Rehak, Nicola Maffulli, Giovanna Della Porta
Summary: In this study, a 3D environment was created using fibrin scaffold and two cell populations. The addition of peripheral blood mononuclear cells (PBMCs) to the culture system significantly influenced myogenesis. The best cell ratio for the 3D culture was found to be 1:1 (BM-MSCs plus SkMs:PBMCs), resulting in enhanced viability and myogenic gene expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Ying-Gui Yang, Young Wha Koh, Ita Novita Sari, Nayoung Jun, Sanghyun Lee, Lan Thi Hanh Phi, Kwang Seock Kim, Yoseph Toni Wijaya, Sang Hun Lee, Moo-Jun Baek, Dongjun Jeong, Hyog Young Kwon
INTERNATIONAL JOURNAL OF CANCER
(2019)
Article
Biochemistry & Molecular Biology
Eun Ji Kang, Yu Mi Baek, Eunil Hahm, Sang Hun Lee, Xuan-Hung Pham, Mi Suk Noh, Dong-Eun Kim, Bong-Hyun Jun
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Endocrinology & Metabolism
Yong-Seok Han, Sang Min Kim, Jun Hee Lee, Seo Kyung Jung, Hyunjin Noh, Sang Hun Lee
JOURNAL OF PINEAL RESEARCH
(2019)
Article
Telecommunications
Hyun-Ho Han, Seuc-Ho Ryu, Gyoo-Soo Chae, Sang-Hun Lee
WIRELESS PERSONAL COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Jun Hee Lee, Yeo Min Yoon, Sang Hun Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Chul Won Yun, Sang Hun Lee
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Chemistry, Physical
Xuan-Hung Pham, Eunil Hahm, Eunji Kang, Yu Na Ha, Sang Hun Lee, Won-Yeop Rho, Yoon-Sik Lee, Dae Hong Jeong, Bong-Hyun Jun
JOURNAL OF ALLOYS AND COMPOUNDS
(2019)
Article
Oncology
Hyun Jin Roh, Eun Byeol Go, Kyung Bin Kim, Jong Hwa Lee, Sang Hun Lee
ANTICANCER RESEARCH
(2019)
Review
Medicine, General & Internal
Jong Hwa Lee, Hyun Jin Roh, Jun Woo Ahn, Jeong Sook Kim, Jin Young Choi, Soo-Jeong Lee, Sang Hun Lee
JOURNAL OF CLINICAL MEDICINE
(2020)
Review
Medicine, General & Internal
Jin-Young Choi, Song-Soo Yang, Jong-Hwa Lee, Hyun-Jin Roh, Jun-Woo Ahn, Jeong-Sook Kim, Soo-Jeong Lee, Sang-Hun Lee
Summary: Congenital diaphragmatic hernia, especially Bochdalek hernia, during pregnancy is rare but can lead to high morbidity and mortality. Early diagnosis and surgical intervention are crucial, with multidisciplinary care and individualized treatment being key to optimal results for both the mother and fetus.
Review
Medicine, General & Internal
Ok Ju Kang, Ji Hye Koh, Ji Eun Yoo, So Yeon Park, Jeong-Ik Park, Songsoo Yang, Sang-Hun Lee, Soo-Jeong Lee, Jun-Woo Ahn, Hyun-Jin Roh, Jeong Sook Kim
Summary: Non-tubal ectopic pregnancies with abdominal hemorrhaging have significantly higher mortality and morbidity rates compared to tubal pregnancies. Prompt diagnosis and management through laparoscopic surgery are crucial for improving prognosis in cases of primary diaphragmatic ectopic pregnancy with hemorrhage.
Review
Medicine, General & Internal
Hyoeun Kim, Ji Hye Koh, Jihee Lee, Yeongeun Sim, Sang-Hun Lee, Soo-Jeong Lee, Jun-Woo Ahn, Hyun-Jin Roh, Jeong Sook Kim
Summary: This case report presents a successful treatment for heterotopic cesarean scar pregnancy, using selective potassium chloride injection into the scar pregnancy and uterine cerclage to control vaginal bleeding, resulting in the preservation of the intrauterine pregnancy and full-term delivery.
Review
Medicine, General & Internal
Sang Hun Lee, Songsoo Yang, Inkyu Park, Yeong Cheol Im, Gyu Yeol Kim
Summary: This case report describes a 33-year-old pregnant woman who experienced a sudden onset of epigastric pain at 18 weeks of pregnancy due to a ruptured SAA. After emergent intervention and surgery, the woman recovered without complications and was discharged 15 days postoperatively, even though the fetus was found to be dead during resuscitation.
WORLD JOURNAL OF CLINICAL CASES
(2022)
Article
Urology & Nephrology
Seong Cheol Kim, Won Hyeok Lee, Song Hee Kim, Abdumadjidov Alisher Abdulkhayevich, Jeong Woo Park, Young Min Kim, Kyung Hyun Moon, Sang Hun Lee, Sungchan Park
Summary: This study confirms the association between DRG2 expression and docetaxel-induced apoptosis in prostate cancer cells. Knockdown of DRG2 increases docetaxel-induced apoptosis without G2/M arrest, showing potential to enhance treatment outcomes in castration-resistant prostate cancer.
INVESTIGATIVE AND CLINICAL UROLOGY
(2021)
Article
Oncology
Jeong-Yeol Park, Sang-Hun Lee, Kyu-Rae Kim, Young-Tak Kim, Joo-Hyun Nam
JOURNAL OF GYNECOLOGIC ONCOLOGY
(2019)