期刊
THERIOGENOLOGY
卷 108, 期 -, 页码 362-370出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2017.12.017
关键词
Dietary energy level; AMPK; Autophagy; Testis; Hu sheep
资金
- Natural Science Foundation of Jiangsu Province [BK20161444]
- Chinese Fundamental Research Funds for the Central Universities [KYDK201702]
- Special Found for Agro-scientific Research in the Public Interest [201303144]
Energy balance is an important feature for spermatozoa production in the testis. The 5'-AMP-activated protein kinase (AMPK) is a sensor of cell energy, has been implicated as a mediator between gonadal function and energy balance. Herein, we intended to determine the physiological effects of AMPK on testicular development in feed energy restricted and compensated pre-pubertal rams. Lambs had restricted feeding for 2 months and then provided compensatory feeding for another 3 months. Feed levels were 100%(control), 15% and 30% of energy restriction (ER) diets, respectively. The results showed that lambs fed the 30% ER diet had significantly lower testicular weight (P < .05) and spermatids number in the seminiferous tubules, but there were no differences between control and 15% ER groups. Mean-while, 15% ER and 30% ER diets induced testis autophagy and apoptosis through activating AMPK-ULK1(ULK1, Unc-51 like autophagy activating kinase) signal pathway with characterization of increased Beclin-1 and Light chain 3-II/Light chain 3-I (LC3-II/LC3-I) ratio, up-regulated the ratio of proapoptotic Bcl-2-associated X protein (BAX) and anti-apoptotic B-cell lymphoma 2 (Bcl-2), as well as activated AMPK, phosphorylated AMPK(p-AMPK) and ULK1. Furthermore, a compensation of these parameters occurred when the lambs were re-fed with normal energy requirement after restriction. Taken together, dietary energy levels influence testicular development through autophagy and apoptosis interplay mediated by AMPK-ULK1 signal pathway, which also indicates the important role of the actions of AMPK in the testis homeostasis. (C) 2017 Elsevier Inc. All rights reserved.
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