Article
Agriculture, Dairy & Animal Science
Claudia Lima Verde Leal, Karina Canon-Beltran, Yulia N. Cajas, Meriem Hamdi, Aracelli Yaryes, Maria Gemma Millan de la Blanca, Paula Beltran-Brena, Rosane Mazzarella, Juliano Coelho da Silveira, Alfonso Gutierrez-Adan, Encina M. Gonzalez, Dimitrios Rizos
Summary: This study aimed to evaluate the effect of extracellular vesicles (EVs) isolated from oviductal fluid (OF) and uterine fluid (UF) on the development and quality of bovine embryos in vitro. The results showed that the use of EVs improved blastocyst quality, including survival rate after vitrification/warming, total cell number, lipid content, and the expression of lipid metabolism-related genes. Additionally, the miRNA contents in EVs may contribute to these observed effects.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
Song Gao, Zheng Wang, Jukui Ma, Jingyi Wang, Xupeng Xing, Chenglong Shen, Zhihan Niu, Heqiang Li, Shaowei Zhang, Kaijuan Zhang, Jing Gao, Yong Zhang, Yongsheng Wang
Summary: Recent studies have shown that abnormal cytoskeletal changes are an important cause of developmental failure in somatic cell nuclear transfer (SCNT) embryos, in addition to alterations in abnormal chromosome epigenetic modifications. In this study, the effects of ACY-1215, a specific inhibitor of HDAC6, on the acetylation of alpha-tubulin, histone epigenetic modification, spindle formation, and embryonic development of early bovine SCNT embryos were investigated. The results showed that ACY-1215 treatment improved the acetylation level of alpha-tubulin and histone proteins, corrected spindle abnormalities, delayed cleavage timing, increased cell numbers, and reduced apoptosis in SCNT blastocysts. ACY-1215 was found to regulate the process of embryonic epigenetic modification and cytoskeletal protein acetylation, leading to improved SCNT embryonic development potential.
Article
Agriculture, Dairy & Animal Science
Juan P. Cabeza, Juan Camera, Olinda Briski, Minerva Yauri Felipe, Daniel F. Salamone, Andres Gambini
Summary: This study investigates the use of a novel zinc chelator for activating bovine and porcine oocytes. The results show that the zinc chelator can successfully induce blastocyst development in both species, although developmental rates and embryo quality are compromised in bovine compared to calcium-induced embryos. On the contrary, the new concentration and incubation time of the zinc chelator improve developmental rates in porcine without affecting embryo quality. These findings contribute to understanding the role of zinc in oocyte activation and preimplantation embryo development across different mammalian species.
Article
Endocrinology & Metabolism
Jingyu Li, Shun Xiong, Yanhua Zhao, Chong Li, Wei Han, Guoning Huang
Summary: The re-vitrification of embryos at different stages has varying effects on delivery rates, with re-vitrification at the blastocyst stage showing a decrease in potential compared to re-vitrification at the 8-cell stage. The expression levels of BAX and CASPASE3 were significantly higher in re-vitrified groups.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Reproductive Biology
F. Magata, M. Urakawa, F. Matsuda, Y. Oono
Summary: The use of sexed semen to produce bovine embryos results in embryos with slower growth, reduced cell numbers, and decreased viability compared to embryos produced with unsorted semen. These differences may be attributed to abnormalities during the first cleavage and could explain the reduced fertility observed in embryos derived from sexed semen.
Article
Biotechnology & Applied Microbiology
Dessie Salilew-Wondim, Dawit Tesfaye, Franca Rings, Eva Held-Hoelker, Dennis Miskel, Marc-Andre Sirard, Ernst Tholen, Karl Schellander, Michael Hoelker
Summary: The study compared the transcriptome profiles of in vivo and in vitro derived bovine blastocysts, revealing the effects of developmental competence and environmental conditions on gene expression outlines. It also identified differentially expressed genes and pathways reflective of developmental capacities, suggesting that transcriptome activity in blastocysts may indicate future success in pregnancy and adaptation to culture environment.
Article
Biochemistry & Molecular Biology
Krzysztof Lukaszuk, Amira Podolak
Summary: This retrospective case control study found that mtDNA copy number is higher in aneuploid embryos compared to euploid embryos, but no significant differences were observed in relation to embryo morphology, genetic sex, maternal age, or implantation ability. Therefore, mtDNA has the potential to serve as an additional independent biomarker for embryo selection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Giovana D. Catandi, Ming-Hao Cheng, Adam J. Chicco, Tom Chen, Elaine M. Carnevale
Summary: Maternal obesity increases NEFA follicular concentrations and affects mitochondrial metabolism and embryo quality. Supplementation with L-carnitine during in vitro maturation of bovine COCs exposed to high NEFA can mitigate the negative effects of excessive lipid exposure on embryonic development and oxidative stress.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Biochemical Research Methods
Johanna Piibor, Andres Waldmann, Keerthie Dissanayake, Aneta Andronowska, Marilin Ivask, Madhusha Prasadani, Ants Kavak, Suranga Kodithuwakku, Alireza Fazeli
Summary: This study used quantitative proteomics-based mass spectrometry to investigate the changes in the proteome of extracellular vesicles (EVs) in the uterine fluid (UF) during the bovine estrous cycle. The results showed that the UF-EV proteome is cycle-dependent and differentially enriched proteins play important roles in normal uterine functions and early embryo development. Furthermore, supplementation of UF-EVs during the luteal phase increased embryo production in vitro. These findings highlight the importance of UF-EVs in uterine communications and their potential use in improving embryo production.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Cell Biology
Chi-Hun Park, Young-Hee Jeoung, Luhui Zhang, Sai Goutham Reddy Yeddula, Ki-Eun Park, Jerel Waters, Bhanu P. P. Telugu
Summary: EFs derived from porcine embryonic outgrowths exhibit similarities to FFs. EFs can be used as nuclear donors in SCNT to enhance post-blastocyst development, implantation, and pregnancy outcomes, and as a source for CRISPR/Cas genome editing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Hao Li, Shengnan Chen, Shanhua Piao, Tiezhu An, Chunsheng Wang
Summary: The study cloned a high glycine/tyrosine protein gene (HGT) promoter from sheep by PCR, constructed a pcDNA3.1-HGT-4S eukaryotic expression vector, and successfully obtained cloned embryos of transgenic sheep with synthetic spidroin gene through SCNT. Further research is needed to explore transgenic sheep expressing synthetic spidroin genes in hair follicles.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Cell Biology
Deling Jiao, Wenmin Cheng, Xiaolin Zhang, Yifan Zhang, Jianxiong Guo, Zhuo Li, Dejia Shi, Zhe Xiong, Yubo Qing, Muhammad Ameen Jamal, Kaixiang Xu, Hong-Ye Zhao, Hong-Jiang Wei
Summary: The study found that small PFFs with a diameter of less than 13 μm are more suitable donor cells for somatic cell nuclear transfer (SCNT) in pigs. Small cells exhibited better growth status and proliferation capabilities compared to medium and large cells, with medium and large cells showing various abnormalities such as cell membrane protuberance, karyo-pyknosis, autophagy, and mitochondrial abnormalities.
Article
Agriculture, Dairy & Animal Science
Yiran Wei, Muhammad Idrees, Tabinda Sidrat, Myeondon Joo, Lianguang Xu, Jonghyeok Ko, Ilkeun Kong
Summary: The study demonstrates that the addition of exosomes derived from bovine oviductal epithelial cells (BOEC-Exo) into in vitro maturation media significantly enhances bovine oocyte maturation and early embryo development, reducing apoptosis and improving blastocyst implantation potential.
Article
Agriculture, Dairy & Animal Science
Xiao Wu, Huaxing Zhao, Junkun Lai, Ning Zhang, Junsong Shi, Rong Zhou, Qiaoyun Su, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Zhenfang Wu, Zicong Li
Summary: IL17D protein enhances the developmental ability of cloned pig embryos by inhibiting cell death and up-regulating GADD45B expression; it also reduces cell apoptosis and promotes gene expression in cloned pig embryos, improving the efficiency of somatic cell nuclear transfer embryos.
Article
Biochemistry & Molecular Biology
Guanghua Su, Lina Wang, Guangqi Gao, Shanshan Wu, Lei Yang, Meiling Wu, Xuefei Liu, Miaomiao Yang, Zhuying Wei, Chunling Bai, Guangpeng Li
Summary: In interspecies somatic cell nuclear transfer (iSCNT) embryos, a developmental block in the 8-cell stage was identified, characterized by lower expression of ribosomal subunit genes and abnormal nucleolin structure. Modulating the expression of nucleolin protein (C23) improved blastocyst development in both SCNT and iSCNT embryos, suggesting that abnormal C23 and ribosomal subunit gene expression leads to developmental block and ZGA failure in iSCNT embryos.
Article
Computer Science, Cybernetics
Bingxin Li, Zheng Wei, Tong Yang, Yanfang Liu, Feng Du
Summary: This study compares three list scrolling gestures on a mobile phone: sliding to scroll, moving a regular scrollbar, and scrolling through alphabetic index. It finds that scrolling through alphabetic index is the fastest regardless of target locations. The study also reveals that sliding to scroll has shorter reaction time for near-reaching targets, but longer reaction time for far-reaching targets. The evaluation scores of alphabetic index increase over time, while those of sliding to scroll decrease. The different rating patterns before and after the task are attributed to fatigue and emotional changes. This study provides insights into users' mental models for three list scrolling gestures and their implications for future gesture design.
INTERNATIONAL JOURNAL OF HUMAN-COMPUTER INTERACTION
(2023)
Article
Biodiversity Conservation
Xiaowei Guo, Xiali Mao, Wu Yu, Liujun Xiao, Mingming Wang, Shuai Zhang, Jinyang Zheng, Hangxin Zhou, Lun Luo, Jinfeng Chang, Zhou Shi, Zhongkui Luo
Summary: Soil biogeochemical processes exhibit depth-dependent responses to climate change. This study presents an innovative and cost-effective approach of field incubation of intact soil cores to explore this depth dependence. The results indicate that soil respiration responds significantly to climate shifts induced by translocation experiments, but this response is independent of soil depth.
GLOBAL CHANGE BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Nannan Zhang, Lulu Du, Jianyong Zhang, Hantao Xu, Xuan Zhou, Liqiang Mai, Lin Xu
Summary: A flexible tent-like interface constructed by self-assembly of graphene oxide on zinc nanosheets is proposed to achieve stable lithium metal anode. This interface immobilizes the graphene oxide layer and enables homogeneous lithium deposition within nanocavities. The confinement of Li plating/stripping inside the nanocavities effectively reduces electrolyte-Li reaction and eliminates continuous consumption of Li metal, leading to excellent cycling performance and high-capacity retention.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Engineering, Environmental
Lulu Du, Bo Zhang, Xiaofang Wang, Chenhui Dong, Liqiang Mai, Lin Xu
Summary: Solid-state lithium metal batteries (LMBs) have promising prospects due to their high energy density and safety. Developing high-performance solid electrolytes is crucial for practical application of LMBs. Composite polymer electrolytes (CPEs) consisting of inorganic fillers and polymer electrolytes have gained attention for their improved ionic conductivity and mechanical strength.
CHEMICAL ENGINEERING JOURNAL
(2023)
Letter
Hematology
Sen Yang, Xiaoshuai Zhang, Robert Peter Gale, Xin Du, Chun-yan Chen, Jian-yu Weng, Jian Huang, Fei Li, Yun Zeng, Zhen Xiao, Jian-da Hu, Li-jie Yang, Zhuo-gang Liu, Guo-hui Li, Xiu-li Sun, Wei Yang, Ru Feng, Yan-qiu Han, Yu Jing, Na Xu, Xiao-li Liu, Zhen-fang Liu, Xiao-dong Wang, Shi-xin Wu, Rong Liang, Yan-li Zhang, Yun-fan Yang, Huan-ling Zhu, Ling Pan, Li Meng, Yan-hong Zhao, Hai Yi, Yi-lan Liu, Wei-hua Zhang, Yuan-jun Zheng, Ze-ping Zhou, Su-ning Chen, Hui-ying Qiu, Wei-ming Li, Zhi-lin Jia, Yan-liang Bai, Li-e Lin, Bing-cheng Liu, Chun-shui Liu, Jian-min Luo, Jun-xia Meng, Zhi-qiang Sun, Yan-qing Zhang, Xiao-jun Huang, Qian Jiang
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Engineering, Environmental
Zhe Fan, Hao Sun, Liguang Dou, Shuai Zhang, Wei Han, Cheng Zhang, Tao Shao
Summary: Non-thermal plasma is a promising method for converting heavy oil into value-add chemicals due to its adaptability and high conversion rate. The pulse voltage and repetition frequency of the pulsed spark discharge plasma were found to be the main factors affecting product yields and distribution. The research provides an effective COx-free method for producing H-2, C2H2, and carbon nanomaterials with wide application prospects for heavy oil utilization.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Multidisciplinary
Xiangyuan Meng, Mei Huang, Boxue Wang, Yaodi Li, Yanting Cheng, Chihiro Morita
Summary: The half-boundary method (HBM) is developed for nonlinear convection-diffusion equations (CDEs) and shows excellent performance in simulating flow and heat transfer, especially for convection domination. The HBM reduces the maximum order of matrix and calculation memory storage by utilizing the variable relationship between the nodes inside the domain and the nodes on half of the boundaries. Moreover, it can directly solve discontinuous problems without adding continuity conditions due to the use of mixed variables.
ENGINEERING ANALYSIS WITH BOUNDARY ELEMENTS
(2023)
Article
Chemistry, Applied
Jiangwei Li, Liguang Dou, Yadi Liu, Yuan Gao, Xiucui Hu, Feng Yu, Jiacong Li, Shuai Zhang, Tao Shao
Summary: Low-carbon plasma-catalysis technology shows potential for converting CO2/CH4 into H2 and value-added oxygenates at low temperatures. However, efficiently regulating the product distribution and understanding the catalytic roles of active sites remains a challenge.
FUEL PROCESSING TECHNOLOGY
(2023)
Article
Automation & Control Systems
Ce Zhang, Yu Pan, Shuaihua Zhang, Wei Xu, Muhammad Dilawer Hayat, Jianzhuo Sun, Yun Zhang, Bowen Liu, Jiazhen Zhang, Xin Lu
Summary: Metal injection molding (MIM) is an effective method for preparing brittle TiAl alloy parts. The rheological properties, as-sintered microstructure, interstitial impurities, and mechanical properties of feedstocks using non-spherical powders were systematically studied. By optimizing raw materials and process control, Ti-48Al powder was prepared through self-propagation synthesis. The powder consists of TiAl, Ti3Al, and TiAl3, with an O content of 0.34 wt%. The pseudo-plastic flow behavior and powder loading of feedstock based on different binder systems (POM and PW) were studied. Sintering the non-spherical TiAl powder at 1450°C resulted in samples with a relative density of 96.4%, a tensile strength of 352 MPa, and an elongation of 0.32%, reaching the level of as-cast alloys. Furthermore, the microstructures of samples sintered at various temperatures were compared with the ingot metallurgy TiAl alloy.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2023)
Article
Optics
Liheng Sun, Biao Dong, Jiao Sun, Yue Wang, Rui Sun, Songtao Hu, Bingshuai Zhou, Wen Xu, Xue Bai, Lin Xu, Donglei Zhou, Hongwei Song
Summary: Lead-free double perovskite Cs(2)NaLnX(6) nanocrystals (NCs) with lanthanide elements as structural ions and emission centers exhibit high photoluminescence quantum yield (PLQY) and stability. The broadband emission of Cs(2)NaLnX(6) NCs is attributed to self-trapped excitons. These NCs demonstrate remarkable water stability, temperature stability, and light irradiation stability. The PLQY of these materials is higher than most double perovskite materials, reaching up to 70%. A white light-emitting diode device based on Cs2NaEuTbCl6 shows good color properties. Overall, Cs(2)NaLnX(6) NCs have great potential for future optoelectronic devices.
LASER & PHOTONICS REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Weichao Huang, Anagha Krishnan, Anastasija Plett, Michelle Meagher, Nicole Linka, Yongsheng Wang, Bijie Ren, Justin Findinier, Petra Redekop, Neda Fakhimi, Rick G. Kim, Devin A. Karns, Nanette Boyle, Matthew C. Posewitz, Arthur R. Grossman
Summary: In this study, the researchers identified CreTPT2 and CreTPT3, two proteins involved in the export of photoassimilates from the chloroplast in the green alga Chlamydomonas reinhardtii. They found that CreTPT3 plays a dominant role in this process and is essential for preventing oxidative stress and accumulation of reactive oxygen species in the cell. The study also suggests differences in the export of photoassimilates between Chlamydomonas and vascular plants. The triose phosphate transporter TPT3 is a major distributor of fixed carbon, fueling Chlamydomonas metabolism.
Letter
Computer Science, Information Systems
Fenning Liu, Yue Peng, Wenwu Xiao, Yan Liu, Xiao Yu, Genquan Han
SCIENCE CHINA-INFORMATION SCIENCES
(2023)
Article
Medicine, Research & Experimental
Yichi Zhang, Andres Ramirez-Martinez, Kenian Chen, John R. McAnally, Chunyu Cai, Mateusz Z. Durbacz, Francesco Chemello, Zhaoning Wang, Lin Xu, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
Summary: Mutations in genes encoding nuclear envelope proteins cause nuclear envelopathies, which are characterized by skeletal muscle and heart abnormalities, such as Emery-Dreifuss muscular dystrophy (EDMD). However, the tissue-specific role of the nuclear envelope in these diseases has not been well studied. This study showed that the muscle-specific nuclear envelope protein NET39 is crucial for normal skeletal muscle function and its deficiency leads to key features of EDMD. It was also found that NET39 protects against mechanical stress and DNA damage in muscle cells.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Chemistry, Multidisciplinary
Yefei Jiang, Zhiyong Zeng, Jianhua Yao, Ying Guan, Peipei Jia, Xiaoli Zhao, Lin Xu
Summary: Alzheimer's disease is a neurodegenerative disease characterized by the aggregation of β-amyloid (A,B). Although efforts have been made to control A,B aggregation, no effective medications have been developed. Photodynamic treatment, due to its excellent temporal and spatial selectivity, has been employed to intervene in the aggregation process with some success. This paper reviews the progress of small-molecule photosensitizers in recent years and outlines existing strategies and potential obstacles for enhancing the research and application of photodynamic therapy in Alzheimer's disease.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Physical
Yajing Li, Han Wu, Jinfeng Zhang, Qi Lu, Xiaopeng Han, Xuerong Zheng, Yida Deng, Wenbin Hu
Summary: The development of low-cost, high-performance, and earth-rich electrocatalysts is critical for Zn-air batteries (ZABs). This study provides a simple and quick method for synthesizing doped metal oxides as highly active catalysts in ZABs. By doping Fe, Mn, Ni, Zn, and Mg in metal oxides, the OER and ORR performance of CoO is significantly improved.
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.