期刊
THERAPEUTIC DRUG MONITORING
卷 40, 期 3, 页码 369-376出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/FTD.0000000000000509
关键词
anticoagulants; automated; serum; UHPLC-MS/MS
Background: Direct oral anticoagulants (DOACs) are prescribed for anticoagulation in patients with atrial fibrillation and venous thromboembolic disease. Fixed doses are recommended, but measuring their serum drug concentrations as a basis for dose adjustments may he useful in some clinical settings. Methods: An ultra-high-performance liquid chromatography-tandem mass spectrometry method for the analysis of the DOACs apixaban, dabigatran, edoxaban, and rivaroxaban in human serum was developed and validated. A 100-4 serum sample was handled using a pipetting robot. Protein precipitation was performed with 375 mu L of 1% formic acid in acetonitrile (vol/vol), and phospholipid removal was performed using a Waters Ostro 96-well plate. The injection volume was 1 mu L, and run time was 3.0 minutes. Results: The calibration range was 5-800 nmol/L. The betweenday precision relative SDs were in the range of 3.3%-10%. Recoveries ranged from 85% to 105%, and matrix effects from 88% to 102%, when corrected with internal standard. Edoxaban was, in contrast to the other DOACs, unstable when stored for more than 6 hours at 30 degrees C. The suitability of the method was demonstrated by analyzing routine samples from 345 patients undergoing anticoagulation treatment. Conclusions: The developed method fulfilled the set validation criteria, and its suitability was demonstrated in a routine setting. The instability of edoxaban may complicate the transport of routine samples to the laboratory.
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